Sediment transport remains a significant challenge for researchers due to the intricate nature of the physical processes involved and the diverse characteristics of watercourses worldwide. A type of watercourse that is of particular interest for study is the ephemeral streams, found primarily in semiarid and arid regions. Due to their unique nature, a new measurement algorithm was created and a modified bed load sampler was built. Measurement of the bed load transport rate and calculation of the water discharge were conducted in an ephemeral stream in Northeastern Greece, where the mean calculated streamflow rate ranged from 0.019 to 0.314 m3/s, and the measured sediment load transport rates per unit width varied from 0.00001 to 0.00213 kg/m/s. The sediment concentration was determined through various methods, including nonlinear regression equations and formulas developed by Yang, with the coefficients of these formulas calibrated accordingly. The results demonstrated that the equations derived from Yang’s multiple regression analysis offered a superior fit compared to the original equations. As a result, two modified versions of Yang’s stream sediment transport formulas were developed and are presented to the readership. To assess the accuracy of the modified formulas, a comparison was conducted between the calculated total sediment concentrations and the measured total sediment concentrations based on various statistical criteria. The analysis shows that none of Yang’s original formulas fit the available data well, but after optimization, both modified formulas can be applied to the specific ephemeral stream. The results indicate also that the formulas derived from the nonlinear regression can be successfully used for the determination of the total sediment concentration in the ephemeral stream and have a better fit compared to Yang’s formulas. The correlation from the nonlinear regression equations suggests that total sediment transport is primarily influenced by water discharge and rainfall intensity, with the latter showing a high correlation coefficient of 0.998.