Fitness Measures Research Articles

When does antimicrobial resistance increase bacterial fitness? Effects of dosing, social interactions, and frequency dependence on the benefits of AmpC β-lactamases in broth, biofilms, and a gut infection model.

One of the longstanding puzzles of antimicrobial resistance is why the frequency of resistance persists at intermediate levels. Theoretical explanations for the lack of fixation of resistance include cryptic costs of resistance or negative frequency-dependence but are seldom explored experimentally. β-lactamases, which detoxify penicillin-related antibiotics, have well-characterized frequency-dependent dynamics driven by cheating and cooperation. However, bacterial physiology determines whether β-lactamases are cooperative, and we know little about the sociality or fitness of β-lactamase producers in infections. Moreover, media-based experiments constrain how we measure fitness and ignore important parameters such as infectivity and transmission among hosts. Here, we investigated the fitness effects of broad-spectrum AmpC β-lactamases in Enterobacter cloacae in broth, biofilms, and gut infections in a model insect. We quantified frequency- and dose-dependent fitness using cefotaxime, a third-generation cephalosporin. We predicted that infection dynamics would be similar to those observed in biofilms, with social protection extending over a wide dose range. We found evidence for the sociality of β-lactamases in all contexts with negative frequency-dependent selection, ensuring the persistence of wild-type bacteria, although cooperation was less prevalent in biofilms, contrary to predictions. While competitive fitness in gut infections and broth had similar dynamics, incorporating infectivity into measurements of fitness in infections significantly affected conclusions. Resistant bacteria had reduced infectivity, which limited the fitness benefits of resistance to infections challenged with low antibiotic doses and low initial frequencies of resistance. The fitness of resistant bacteria in more physiologically tolerant states (in biofilms, in infections) could be constrained by the presence of wild-type bacteria, high antibiotic doses, and limited availability of β-lactamases. One conclusion is that increased tolerance of β-lactams does not necessarily increase selection pressure for resistance. Overall, both cryptic fitness costs and frequency dependence curtailed the fitness benefits of resistance in this study.

Reinforcement learning informs optimal treatment strategies to limit antibiotic resistance

Antimicrobial resistance was estimated to be associated with 4.95 million deaths worldwide in 2019. It is possible to frame the antimicrobial resistance problem as a feedback-control problem. If we could optimize this feedback-control problem and translate our findings to the clinic, we could slow, prevent, or reverse the development of high-level drug resistance. Prior work on this topic has relied on systems where the exact dynamics and parameters were known a priori. In this study, we extend this work using a reinforcement learning (RL) approach capable of learning effective drug cycling policies in a system defined by empirically measured fitness landscapes. Crucially, we show that it is possible to learn effective drug cycling policies despite the problems of noisy, limited, or delayed measurement. Given access to a panel of 15 [Formula: see text]-lactam antibiotics with which to treat the simulated Escherichia coli population, we demonstrate that RL agents outperform two naive treatment paradigms at minimizing the population fitness over time. We also show that RL agents approach the performance of the optimal drug cycling policy. Even when stochastic noise is introduced to the measurements of population fitness, we show that RL agents are capable of maintaining evolving populations at lower growth rates compared to controls. We further tested our approach in arbitrary fitness landscapes of up to 1,024 genotypes. We show that minimization of population fitness using drug cycles is not limited by increasing genome size. Our work represents a proof-of-concept for using AI to control complex evolutionary processes.

Optimal homeostatic stress to maximize the homogeneity of adaptations to interval interventions in soccer players.

This study examined the uniformity of adaptations in cardiorespiratory fitness and bio-motor abilities by analyzing individual responses to measures representing the mentioned qualities. Twenty-four male well-trained soccer players (Age = 26 ± 4years; stature = 181 ± 3.8; Weight = 84 ± 6.1) were randomized to two groups performing short sprint interval training [sSIT (3 sets of 10 × 4s all-out sprints with 20s of recovery between efforts and 3min of rest intervals between sets)] or a time-matched small-sided game [SSG (3 sets of 3 v 3 efforts in a 20 × 15m area with 3min of relief in-between)]. Before and after the 6-week training period, aerobic fitness indices, cardiac hemodynamics, and anaerobic power were assessed through a graded exercise test utilizing a gas collection system, noninvasive impedance cardiography, and a lower-body Wingate test, respectively. Also, sport-specific bio-motor abilities were determined by measuring linear speed, change of direction, and jumping ability. Comparing inter-individual variability in the adaptive changes by analyzing residuals in individual adaptations indicated that sSIT induces more uniform changes in the first and second ventilatory threshold (VT1 & VT2), stroke volume, and peak power output across team members than SSG. SSG also yielded lower proportions of responders in , VT1, VT2, peak, and average power output compared to sSIT. Additionally, the coefficient of variation in mean group changes in measures of aerobic fitness and bio-motor abilities in response to sSIT were lower than in SSG. Short sprint interval training induces more homogenized adaptations in measures of cardiorespiratory fitness and anaerobic power than small-sided games across team members.

Are physical activity levels, cardiorespiratory fitness and peak power associated with Parkinson's disease severity?

IntroductionIncreased physical activity (PA) may slow Parkinson's disease (PD) progression. Associations between markers of PA and PD severity could justify further studies evaluating interventions increasing PA levels in PD. The objectives of the present study were to assess associations between PA, cardiorespiratory fitness (VO2-max), and muscle peak power and measures of the Movement Disorder Society-sponsored revision of the Unified Parkinson's Disease Rating Scale (MDS-UPDRS), the Parkinson's disease questionnaire-39 (PDQ-39), and the four PD hallmark motor symptoms (rigidity, bradykinesia, postural instability, and tremor). MethodsData from 105 people with PD were used. PA was measured for seven consecutive days using accelerometers. Peak power was measured with a linear encoder during a chair rise test, while VO2-max was directly assessed during a graded bicycle test. Analyses included simple and multiple linear regression and hurdle exponential regression. ResultsPA was weakly to moderately associated with MDS-UPDRS II + III, rigidity, bradykinesia, and postural instability, as well as PDQ-39 mobility and activities of daily living sub-scores. VO2-max and peak power were weakly to moderately associated with MDS-UPDRS III, bradykinesia, and postural instability, while peak power was further weakly associated with the MDS-UPDRS II. Lastly, VO2-max was associated with PDQ-39 mobility and activities of daily living sub-scores. ConclusionPA, VO2-max, and peak power were associated with PD severity, thus highlighting the potential benefits of a physically active lifestyle. Furthermore, PA and VO2-max were associated with PDQ-39 sub-scores. This calls for confirmation of the potential effect of PA on quality of life in PD.

Effects of a horizontal speed deceleration training programme on measures of physical fitness in youth male handball players

ABSTRACT This study examined the effects of an 8-week horizontal speed deceleration training (HSDT) programme in combination with regular handball-specific training as compared with handball-specific training only in measures of physical fitness in male youth handball players. Thirty-nine players were randomly assigned to either an HSDT group (n = 18; 15.55 ± 0.24 years) or an active-control group (CG; n = 21; 14.59 ± 0.23 years). The results showed significant and large between-group differences at post-test in countermovement jump, change-of-direction speed, and repeated sprint ability (RSA) (all p < 0.01; d = 2.04 and 1.37, 1.39, 1.53, and 1.53 for the CMJ, 505 CoD, RSAbest, RSAaverage, and RSAtotal performances, respectively). The post-hoc-analysis demonstrated significant and large improvements in all measures of physical fitness in the HSDT group (∆2.49% to 16,25%; d = 1.01 to 1,70; all p < 0,01). The CG, however, failed to reach any significant difference in all measures of physical fitness ((∆0.31% to 1.98%; d = 0.15 to 0.22; p = 0.379; p > 0.05). To summarise, an 8-week in-season HSDT programme alongside regular handball-specific training yielded positive effects on various performance measures including jumping ability, CoD speed, and RSA, when compared to handball-specific training alone. These results highlight the potential benefits of integrating HSDT into the training regimen of youth handball athletes during the competitive season.

Reliability in Novel Field-Based Fitness Measurements and Postexercise Scores from a Physical Fitness Test Battery in Older Adults

Introduction: Physical fitness is strongly associated with daily physical function, health, and longevity in older adults. Field-based tests may provide a reasonable alternative compared to advanced laboratory testing. Separating postexercise test scores from reactivity measurements requires sufficient test-retest reliability. Postexercise test scores with reliability analyses of field-based fitness tests in older adults are lacking. The present study aimed to examine the test-retest reliability of some novel easily accommodated fitness test measurements and compare pretest scores with postexercise results in these tests along with other field-based fitness tests in older adults. Methods: Totally 1,407 community-dwelling older adults (69% female), x̄ = 71.5 ± 5.0 (65–84 years), performed twelve field-based fitness tests at pretest 1, pretest 2 and a posttest after an 8-week exercise period (twice weekly 1 h of combined strength and aerobic training). T tests, intra-class correlation, limits of agreement, standard error of measurement, and coefficient of variance were performed between pre-1 and pre-2 tests, and repeated measures ANOVA and partial eta squared effect size for postexercise differences for men and women in 5-year age groups ranging from 65 to 84 years. Results: Between pre-1 and pre-2 tests a significant difference was noted in some of the novel fitness test measurements but generally not, e.g., in isometric trunk flexion and step-up height on either leg among all sex and age groups. In most of these novel fitness test measurements, no significant differences occurred between the two pretests. Examples of results from the pre-2 test to the posttest were isometric trunk flexion 45° endurance and isometric trunk extension endurance improved significantly for both sexes in age groups 65–74 years. Women, but not men, improved the maximal step-up height for both legs in most age groups. The speed in the 50 sit-to-stand improved significantly for most age groups in both sexes. Six-min walk distance improved significantly for most age groups in women but among men only in 65–69 years. In the timed-up-and-go test, significant improvements were seen for all age groups in women and in men 70–79 years. No postexercise improvements were generally observed for grip strength or balance. Conclusions: In most of the novel fitness test measures, no significant difference was noted between the two pretests in the assessed sex and age groups. Results after the 8-week exercise period varied between sex and age groups, with significant improvements in several of the twelve studied fitness tests. These findings may be valuable for future projects utilizing easily accommodated physical fitness tests in older adults.

Feeding Drosophila gut microbiomes from young and old flies modifies the microbiome

It is becoming increasingly evident that the myriad of microbes in the gut, within cells and attached to body parts (or roots of plants), play crucial roles for the host. Although this has been known for decades, recent developments in molecular biology allow for expanded insight into the abundance and function of these microbes. Here we used the vinegar fly, Drosophila melanogaster, to investigate fitness measures across the lifetime of flies fed a suspension of gut microbes harvested from young or old flies, respectively. Our hypothesis was that flies constitutively enriched with a ‘Young microbiome’ would live longer and be more agile at old age (i.e. have increased healthspan) compared to flies enriched with an ‘Old microbiome’. Three major take home messages came out of our study: (1) the gut microbiomes of young and old flies differ markedly; (2) feeding flies with Young and Old microbiomes altered the microbiome of recipient flies and (3) the two different microbial diets did not have any effect on locomotor activity nor lifespan of the recipient flies, contradicting our working hypothesis. Combined, these results provide novel insight into the interplay between hosts and their microbiomes and clearly highlight that the phenotypic effects of gut transplants and probiotics can be complex and unpredictable.

Maximal cardiopulmonary exercise testing in glioblastoma patients undergoing chemotherapy: assessment of feasibility, safety, and physical fitness status.

Maximal cardiopulmonary exercise testing (max. CPET) provides the most accurate measurement of cardiorespiratory fitness. However, glioblastoma (GBM) patients often undergo less intensive tests, e.g., 6-min walk test or self-rating scales. This study aims to demonstrate feasibility and safety of max. CPET in GBM patients, concurrently evaluating their physical fitness status. Newly diagnosed GBM patients undergoing adjuvant chemotherapy were offered participation in an exercise program. At baseline, max. CPET assessed cardiorespiratory fitness including peak oxygen consumption (VO2peak), peak workload, and physical work capacity (PWC) at 75% of age-adjusted maximal heart rate (HR). Criteria for peak workload were predefined based on threshold values in HR, respiratory quotient, respiratory equivalent, lactate, and rate of perceived effort. Data were compared to normative values. Adverse events were categorized according to standardized international criteria. Further, self-reported exercise data pre- and post-diagnosis were gathered. All 36 patients (median-aged 60; 21 men) met the predefined criteria for peak workload. Mean absolute VO2peak was 1750 ± 529 ml/min, peak workload averaged 130 ± 43 W, and mean PWC was 0.99 ± 0.38 W/kg BW, all clinically meaningful lower than age- and sex-predicted normative values (87%, 79%, 90%, resp.). Only once (3%) a minor, transient side effect occurred (post-test dizziness, no intervention needed). Self-reported exercise decreased from 15.8 MET-h/week pre-diagnosis to 7.2 MET-h/week post-diagnosis. Max. CPET in this well-defined population proved feasible and safe. GBM patients exhibit reduced cardiorespiratory fitness, indicating the need for tailored exercise to enhance health and quality of life. CPET could be essential in establishing precise exercise guidelines.

Investigating the Effectiveness of Six-Week Plyometric Training Intervention on Speed-Strength Fitness Abilities of Male Team Handball Players

Background and study aim: Plyometric training is widely acknowledged as an effective option for athletes to attain improved physical performance. A variety of exercises involving the lower limb's stretch-shortening cycle can be used for plyometric training. Numerous studies have examined the impact of plyometric training on various outcomes in various populations over the past few decades. Objective: This study set out to ascertain how a six-week plyometric training programme affected the development of speed-strength fitness measurements in male team handball athletes. Material and methods: Total of thirty intercollegiate level team handball athletes were randomly assigned to 2 groups i.e. plyometric training group with an average age of 20.7 ± 0.7 years, height of 175.3 ±4.2, weight of 75.1 ± 4.1 and BMI of 24.43 ± 0.97; control group with an average age of 20.8 ± 0.7, height of 175.1 ± 4., weight of 74.9 ± 3.1 and BMI of 24.47 ± 1.37. The linear sprint (10 m and 30 m), standing long jump (SLJ), and change of direction sprint (CODS) were evaluated before and after the intervention for thirty male team handball athletes who were randomly assigned to either the plyometric training group (PTG) (n = 15) or the control group (CG) (n = 15). Results: Within group changes were analyzed using paired t-test. The significant differences obtained between the tests in all dependent variables. Conclusion: When it comes to linear sprints, standing long jumps, and change of direction sprints, plyometric training works better than CG. Consequently, adding either plyometric training or sport-specific training to an athlete's regimen may help male team handball players achieve greater speed-strength fitness.

Responsiveness of respiratory function in Parkinson's Disease to an integrative exercise programme: A prospective cohort study.

Respiratory disorders are the most common cause of death in Parkinson's Disease (PD). Conflicting data exist on the aetiology of respiratory dysfunction in PD and few studies examine the effects of exercise-based interventions on respiratory measures. This study was conducted to better understand respiratory dysfunction in PD and to identify measures of dysfunction responsive to an integrative exercise programme. The objectives were to compare baseline respiratory measures with matched, published population norms and to examine immediate and longer-term effects of a 12-week integrated exercise programme on these measures. Twenty-three people with mild PD (median Hoehn & Yahr = 2) self-selected to participate in this exploratory prospective cohort study. Evaluation of participants occurred at three time points: at baseline; following the 12-week exercise programme and at 4-month follow-up. Outcome measures included: Forced Vital Capacity (FVC), Forced Expiratory Volume in 1 second (FEV1), FEV1/FVC ratio, Peak Expiratory Flow (PEF), Inspiratory Muscle Strength (MIP), Expiratory Muscle Strength (MEP), Peak Cough Flow (PCF), and Cardiovascular Fitness measures of estimated VO2 max and 6-Minute Walk Test (6MWT). Compared to published norms, participants had impaired cough, reduced respiratory muscle strength, FEV, FVC, PEF and cardiovascular fitness. Post exercise intervention, statistically significant improvements were noted in MEP, cardiovascular fitness, and PEF. However only gains in PEF were maintained at 4-month follow-up. Significant respiratory dysfunction exists, even in the early stages of PD. Metrics of respiratory muscle strength, peak expiratory flow and cardiovascular fitness appear responsive to an integrative exercise programme.

Adolescent Cardiorespiratory Fitness and Future Work Ability

Although research indicates that low fitness in youth is associated with a higher risk of chronic disability in men, the association of fitness in adolescence with work ability in working men and women remains unknown. To examine the associations of adolescent health-related physical fitness with future work ability. This 45-year observational cohort study, conducted in Finland, examined the direct and indirect longitudinal associations of objectively measured health-related fitness in adolescence (in 1976) with self-reported work ability and sickness absence in early middle age (in 2001) and the Work Ability Index in late middle age (in 2021). A countrywide stratified random baseline sample included fitness measurements for cardiorespiratory fitness (CRF) (running 1.5 km for girls and 2 km for boys), musculoskeletal fitness (MF; standing broad jump and sit-ups for both sexes, pull-ups for boys, and flexed-arm hang for girls), and height and weight, from which body mass index (BMI) was calculated. Structural equation modeling-based path analysis, adjusted for age and sex at baseline and for educational level, work-related physical strain, and leisure-time physical activity in late middle age, was conducted. Data analysis was performed from January to July 2023. Self-reported work ability was measured with structured questions in early middle age and with the validated Work Ability Index in late middle age. The final sample from longitudinal analyses (1207 individuals; 579 [48%] male individuals) consisted of participants with fitness measurement from age 12 to 19 years, and work ability assessment from age 37 to 44 years and/or age 57 to 64 years. Higher adolescent CRF was associated with higher work ability (839 participants; β = 0.12; 95% CI, 0.01 to 0.22; P = .03) and lower sickness absence (834 participants; β = -0.07; 95% CI, -0.12 to -0.02; P = .004) in early middle age and, indirectly, mediated by work ability in early middle age, with a higher work ability at the end of working age (603 participants; β = 0.04; 95% CI, 0.001 to 0.08; P = .04). The results remained consistent in both sexes and after adjustment for the confounders. Neither MF nor BMI was associated with work ability (MF, 1192 participants; β = -0.07; 95% CI, -0.17 to 0.03; BMI, 1207 participants, β = 0.09; 95% CI, -0.004 to 0.19) or sickness absence (MF, 1185 participants, β = 0.02; 95% CI, -0.03 to 0.06; BMI, 1202 participants, β = -0.03; 95% CI, -0.09 to 0.03) in early middle age or with late middle age work ability, mediated by work ability in early middle age (MF, 603 participants, β = -0.02; 95% CI, -0.06 to 0.01; BMI, 603 participants, β = 0.03; 95% CI, -0.004 to 0.07). These findings suggest that low CRF in youth is associated with poor work ability at the middle and end of working life, which highlights the informative and prognostic value of CRF assessment early in youth. Enhancing CRF in the first decades of life might contribute to better work capacity and productivity in the labor force, which would have implications for health, quality of life, society, and the economy.

Benefits of Pair-Bond Duration on Reproduction in a Lifelong Monogamous Cooperative Passerine.

AbstractLong-term social and genetic monogamy is rare in animals except birds, but even in birds it is infrequent and poorly understood. We investigated possible advantages of monogamy in a colonial, facultative cooperatively breeding bird from an arid, unpredictable environment, the sociable weaver (Philetairus socius). We documented divorce and extrapair paternity of 703 pairs over 10 years and separated effects of pair duration from breeding experience by analyzing longitudinal and cross-sectional datasets. Parts of the colonies were protected from nest predation, thereby limiting its stochastic and thus confounding effect on fitness measures. We found that 6.4% of sociable weaver pairs divorced and 2.2% of young were extrapair. Longer pair-bonds were associated with more clutches and fledglings per season and with reproducing earlier and later in the season, when snake predation is lower, but not with increased egg or fledgling mass or with nestling survival. Finally, the number of helpers at the nest increased with pair-bond duration. Results were similar for protected and unprotected nests. We suggest that long-term monogamy is associated with a better capacity for exploiting a temporally unpredictable environment and helps to form larger groups. These results can contribute to our understanding of why long-term monogamy is frequently associated with unpredictable environments and cooperation.

Effects of Fitlight training on cognitive-motor performance in élite judo athletes

AimsThe aims of this study were to verify if a 5-week cognitive-motor training (CMT) using FitlightsTM induced changes in young adult judo athletes compared to a non-intervention group. Specifically, it was verified if CMT influenced executive functions (EFs), physical fitness and brain-derived neurotrophic factor (BDNF) levels. Additionally, athletes’ competitive results were compared between groups. MethodTwenty-seven athletes (14 males and 13 females; age = 19.5 ± 2.0 years) were assigned to the Fitlight (FG) and control (CG) groups which performed 5 weeks of CMT, respectively, including 25 min per day of Fitlight training or traditional judo practice. All participants performed cognitive (flanker task and forward/backward digit span) and fitness tests (counter movement jump, handgrip test, dynamic and isometric chin up). In addition, BDNF was collected by saliva sampling and competitive results after the intervention period were considered. ResultsRM-ANOVA showed significant differences in FG for the accuracy of flanker (p = 0.028) and backward digit span (p < 0.001). Moreover, significant differences in FG were found for relative dynamic chin up (p = 0.027) and counter movement jump (p = 0.05). In addition, a significant difference in FG was found for competitive results after the intervention period (p < 0.01).No significant differences were found for BDNF and other cognitive and fitness measures (p > 0.05). ConclusionA 5-week judo-specific CMT improved EFs and motor performance in élite judo athletes. It seems that CMT with Fitlight™ could be considered an additional support to coaches during the training period.

Off-Pump Coronary Artery Bypass Grafting Does Not Confer Superior Outcomes Among Frail Patients

Off-pump coronary revascularization (OPCAB) has been proposed to benefit patients who are at a greater surgical risk because it avoids the use of extracorporeal circulation. Although, historically, older patients were considered high-risk candidates, recent studies implicate frailty as a more comprehensive measure of perioperative fitness. Yet, the outcomes of OPCAB in frail patients have not been elucidated. Thus, using a national cohort of frail patients, we assessed the impact of OPCAB relative to on-pump coronary revascularization (ONCAB). Patients who underwent first-time elective coronary revascularization were tabulated from the 2010 to 2020 Nationwide Readmissions Database. Frailty was assessed using the previously-validated Johns Hopkins Adjusted Clinical Groups indicator. Multivariable models were used to consider the independent associations between OPCAB and the key outcomes. Of ∼26,529 frail patients, 6,322 (23.8%) underwent OPCAB. After risk adjustment and compared with ONCAB, OPCAB was linked with similar odds of in-hospital mortality but greater likelihood of postoperative cardiac arrest (adjusted odds ratio [AOR] 1.53, confidence interval [CI] 1.13 to 2.07) and myocardial infarction (AOR 1.44, CI 1.23 to 1.69). OPCAB was further associated with greater odds of postoperative infection (AOR 1.22, CI 1.02 to 1.47) but decreased need for blood transfusion (AOR 0.68, CI 0.60 to 0.77). In addition, OPCAB faced a +0.86-day increase in length of stay (CI 0.21 to 1.51) but similar costs (β $1,610, CI -$1,240 to 4,460) relative to ONCAB. Although OPCAB was associated with no difference in mortality compared with ONCAB, it was linked with greater likelihood of postoperative cardiac arrest and myocardial infarction. Our findings demonstrate that ONCAB remains associated with superior outcomes, even in the growing population of frail patients who underwent coronary revascularization.

Field-based fitness measures improve via an immersive virtual reality exergaming platform: a randomized controlled trial

While there has been a recent onslaught of traditional lab-based fitness measures in immersive virtual reality (IVR) exergaming research, there remains a paucity in the field-based fitness domain, which refers to assessments made outside a formal laboratory setting which are easier, cheaper, and have more practical application. This study aimed to assess changes in field-based fitness tests including the 1-mile run, 20-m dash, multiple single-leg hop-stabilization test, Abalakov jump, and 5-10-5 Pro Agility test during a 1-month workout protocol and to compare differences between groups assigned to either an IVR machine-directed exergaming platform or a traditional, self-directed cable-resistance training control (SELF). Eighteen (7 females) college-aged participants with little resistance training experience were randomized to IVR or SELF and worked out thrice weekly for 4 weeks (12 sessions). Wilcoxon rank-sum tests were performed for continuous variables to assess significance. Compared to SELF, the IVR group had significantly better performance improvements in 20 m dash (−0.1s vs. 0.0s, p = 0.022), 5-10-5 Pro Agility Test (−0.1s vs. −0.0s, p = 0.003), Abalakov Jump (5.8 cm vs. 2.0 cm, p = 0.0013), 1-Mile Run (−11.0s vs. −2.0s, p = 0.008), and Multiple Single-Leg Hop-Stabilization Test with their dominant (−9.0s vs. 1.0s, p = 0.0015) and non-dominant (−8.0s vs. 1.0s, p = 0.003) legs. This training study demonstrates that IVR exergaming, more so than those that traditionally resistance train (SELF), can improve many field-based fitness components including agility, balance and stability, speed/acceleration, cardiovascular endurance, and lower-body power.

Association of Dietary Fiber and Measures of Physical Fitness with High-Sensitivity C-Reactive Protein.

Dietary fiber intake and physical fitness are independently associated with high-sensitivity C-reactive protein (hs-CRP) levels. Nevertheless, the association between dietary fiber intake, measures of physical fitness, and hs-CRP levels has not yet been fully evaluated. We investigated the influence of a combination of dietary fiber intake and measures of physical fitness, including hand grip strength, resistance training, and metabolic equivalents of tasks, on hs-CRP levels. Data collected from the Korea National Health and Nutrition Examination Survey (KNHANES) spanning 2015 to 2018 were used in this study. A total of 16,934 participants (7434 men and 9500 women aged ≥19 years) were included in this study. After adjusting for confounding factors (age, education, income, marital status, smoking status, drinking habits, total energy intake, and aerobic physical activity), we employed a multivariable logistic model to examine the association of dietary fiber intake and measures of physical fitness with hs-CRP levels. Among women, the odds of high hs-CRP levels were lower in those with the highest dietary fiber intake and superior grip strength compared to in women with the lowest dietary fiber intake and weaker grip strength (odds ratio [OR] = 0.40, 95% confidence interval [CI] = 0.24-0.68). The highest dietary fiber intake who participated in resistance exercise at least three times per week had a reduced odds of high hs-CRP levels compared with those with the lowest dietary fiber intake who did not engage in resistance exercise in both men and women (OR = 0.53, 95% CI = 0.32-0.89; OR = 0.40, 95% CI = 0.19-0.84, respectively). Our findings indicate that dietary fiber intake and high levels of physical fitness were associated with reduced odds of elevated hs-CRP levels.

Standard methods to estimate strength parameters, flight activity, comb construction, and fitness of Apis mellifera colonies 2.0

This paper describes methods for estimating honey bee (Apis mellifera) colony strength by which mean population measures of adult bees and brood are obtained. Additionally, secondary measures, such as the quantity of stored honey and pollen, brood pattern, flight activity, comb construction, and the expression of visible disease or parasite symptoms are addressed. There are generally two contexts in which an investigator wishes to measure colony strength: (1) at the beginning of a study as part of manipulations to produce uniform colonies and reduce experimental error, and (2) as a response variable during or at the end of an experiment. Moreover, there are two general modes for measuring colony strength: (1) an objective mode that uses quantitative measures, and (2) a subjective mode that relies on visual estimates by one or more observers. Other parameters that do not directly measure colony strength are described because they give important indicators of colony state. These parameters include flight activity at the hive entrance, comb construction, and two proxy measures of colony fitness: production of queen cells and drone brood.

Longitudinal Evaluation of Physical Fitness in Adults with Cystic Fibrosis in the Era of CFTR-Modulating-Therapies

ABSTRACT Purpose: Maintaining physical fitness plays an important role in the management of people with cystic fibrosis (pwCF). Longitudinal data on the course of physical fitness and the potential impact of the introduction of highly effective CFTR modulator therapy with elexacaftor/tezacaftor/ivacaftor (ETI) in adult pwCF are scarce. Methods: Health-related and skill-related components of physical fitness were assessed using an incremental cycle test (Wpeak), plus forward bend (FB), prone bent knee hip extension (HE), plank leg raise (PLR), standing long jump (SLJ), and standing on one leg (OLS). Relevant disease-specific clinical data (body mass index [BMI] and forced expiratory volume in 1 second [FEV1]) were recorded. Results: Twenty-eight adult pwCF (age 26.0 ± 7.8 years) were followed over 5.6 ± 0.9 years; 21 started ETI therapy during this period. Significant improvements from baseline were noted in BMI (p < 0.001) and health-related fitness components (HE, p = 0.002; PLR, p = < 0.001), whereas Wpeak and FB remained stable over time (all p > 0.05). Skill-related components (SLJ, OLS) showed no change (all p > 0.05). Subgroup analysis revealed significant improvements in BMI, FEV1, and health-related fitness measures of muscular strength and endurance (HE, p = 0.009; PLR, p < 0.001) only in pwCF using ETI. Conclusion: Despite the improvements, the impact of ETI on the individual parameters was small. Other factors than implementation of ETI alone need to be considered on the way to a high level of physical fitness in adult pwCF.

Adaptive threshold optimisation for online feature selection using dynamic particle swarm optimisation in determining feature relevancy and redundancy

In the era of data-driven decision-making, managing dynamic data streams characterised by evolving data distributions and high dimensionality presents a formidable challenge for online feature selection. This research addresses the challenge by developing innovative solutions in optimising Online Feature Selection (OFS) to manage feature irrelevancy and redundancy and rigorously validating the proposed method in high-dimensional dynamic data streams. The research employs a structured methodology, introducing a novel method: a Dynamic Particle Swarm Optimisation (PSO)-based Threshold Optimisation method. Dynamic PSO, injected on three benchmark methods of OFS (i.e., Online Streaming Feature Selection (OSFS), Fast-OSFS, and Scalable and Accurate Online Feature Selection for Big Data (SAOLA)), enabled the global best position to be dynamically adjusted to exploit the best solution found in streaming data. This research presents two optimisation variants of the proposed method: RedundantPSO, which optimises redundant features, and IrrelevantPSO, which optimises irrelevant features. Unlike the traditional PSO method on feature selection that uses feature encoding representation, the proposed method is underpinned by two contributions: adaptive threshold particle representation of particle swarm optimisation and enhanced fitness function using minimisation of mean absolute deviation of dependency among feature subsets. Adaptive threshold particle representation combines the feature encoding part with a novel aspect that defines a threshold value of significance level ranging from 0.01 to 0.1. This unique contribution sets the research apart in the field where it enables the adaptive adjustment of the threshold value based on incoming features. Next, the adaptation of Mean Absolute Deviation (MAD) was integrated into the fitness evaluation of PSO to gain a more accurate and reliable measure of fitness for feature selection. During the experiment phase, we analysed various benchmark datasets with highly redundant and relevant behaviour. Our analysis concluded that selecting the appropriate threshold values significantly improved model performance for high-redundancy datasets, highlighting the need for careful threshold selection. The experimental evaluations have revealed that integrating RedundantPSO with OSFS (OSFS+RedundantPSO) resulted in a remarkable enhancement of the OSFS method's accuracy, achieving an impressive average accuracy rate of 76.8%. This substantial improvement includes occasional spikes of up to 3.8% over the baseline OSFS accuracy, showcasing OSFS+RedundantPSO as the top-performing combination. Furthermore, Fast-OSFS + RedundantPSO outperformed Fast-OSFS by a slight margin, reaching an average accuracy of 72.7%, while SAOLA + RedundantPSO exhibited a substantial 3.1% increase in average accuracy over SAOLA, reaching 74.0%. It is noteworthy to highlight that the threshold value searched in the proposed method also significantly impacted the identification of the behaviour of the dataset, either high relevancy or redundancy, even in the absence of prior domain knowledge. A higher threshold signifies the evaluation of a more redundant feature space. In conclusion, the results demonstrated the significant contributions of the method in enhancing model accuracy, adapting to evolving data distributions, and optimising feature subsets with acceptable runtime. The research aims to advance the field of data science, such as cybersecurity, finance, healthcare and more, while empowering end-users to make informed decisions under changing data stream circumstances.

Evolutionary origins of Fibonacci phyllotaxis in land plants

Fibonacci phyllotaxis is commonly seen in all major groups of land plants. While a precise correlation is found between the internal pattern of the primary vascular system and the external pattern of appendages on the stem surface, it remains a big question how this regularity of Fibonacci phyllotaxis came into being in the course of evolution. Here I address this problem with a model describing phylogenetic and ontogenetic changes in vascular phyllotaxis based on two hypotheses. The first is that the Fibonacci pattern of vascular connection is uniquely determined by the primary arrangement of incipient primordia, the sources of the primary signal system in vascular tissue differentiation. The second is that the surface-area-to-volume ratio of primary vascular tissues serves as a measure of fitness in evolution. The model explains the empirical rule on the manner in which vascular connection is reconfigured during ontogeny, especially during juvenile development. Fossil and phylogenetic evidence suggests that Fibonacci phyllotaxis appeared shortly after the innovation of indefinite lateral organ initiation in a regular sequence.