Nanoindentation was used to evaluate the effect of three surface preparation techniques—mechanical polishing, electropolishing, and ion polishing—on experimental measurements of incipient plasticity in commercially pure Ni 200. Surface preparation techniques are linked to defect densities, estimated with image quality (IQ) and kernel average misorientation (KAM) data obtained from electron backscatter diffraction patterns and the Taylor relation. Minimum yield pressures are insensitive to surface preparation, while mean yield pressure depends on dislocation density, and the maximum yield pressure is likely influenced by defects other than dislocations. KAM coupled with IQ may be a useful non-destructive parameter to relate surface defect density to the resulting changes in the spatial variability of incipient plasticity during a nanoindentation experiment. This analysis makes the assumption that geometrically necessary dislocation density is proportional to total dislocation density; in cases where this condition is not satisfied, the KAM analysis may not be valid.