Mean Density Research Articles

Hypospadias Reconstruction Training: Development of an Ex-Vivo Model for Objective Evaluation of Surgical Skills

To objectively evaluate technical skill acquisition in hypospadias repair procedures during surgical training using noninvasive wearable sensor technology. We combined subjective video evaluations with objective electromyography (EMG) measurements in a hands-on hypospadias training course. Surgeons wore wireless EMG and accelerometer sensors on their dominant hand while performing tasks on ex-vivo cadaveric calf penises. The study focused on 4 skills as follows: urethral mobilization, dorsal inlay graft harvest/implantation, meatal-based flap urethroplasty, and dorsal plication. Machine learning techniques analyzed muscle activation patterns and attributes for assessing surgical precision. The course included 18 participants (10 female, 8 males; average age 40.18±8.46 years) categorized as novice (n=10, <3 years' experience), intermediate (n=5, 3-5 years), and expert (n=3, >5 years). Video evaluations did not reveal significant differences due to short-term training. However, EMG measurements showed significant reductions in average EMG power, total time, dominant frequency, and cumulative muscle workload after training. Additionally, the mean power spectral density of the EMG signal decreased notably post-training. This study presents a structured approach for hypospadias training and highlights the effectiveness of wearable sensor technology for objective skill assessment. While video evaluations did not detect significant changes, EMG data provided measurable differences in skill acquisition, suggesting that wearable sensors could enhance objective evaluations of surgical proficiency in residency programs.

A retrospective comparative study of extensive sinus membrane perforations repairing using collagen membranes with pouch and nonpouch techniques

ObjectivesTo evaluate the clinical and radiological outcomes of employing collagen membranes with pouch and non-pouch techniques to repair extensive maxillary sinus membrane perforation. Study DesignPatients who experienced extensive maxillary sinus membrane perforation (>10 mm) during lateral window maxillary sinus floor elevation (LWMSFE) were included and treated with either the pouch or non-pouch techniques. ResultsThe number of patients treated with the pouch technique and those treated with the non-pouch technique was 10 each. For the pouch and non-pouch techniques, the mean vertical bone height gain were 17.04±1.64mm and 16.42±2.41mm; the mean bone density were 720.10±20.58HU and 692.85±40.03HU; and the mean bone augmentation volume were 2720.07±680.58mm3 and 3238.84±781.02mm3. The mean vertical bone height gain, bone density and bone augmentation volume were not statistically different between two techniques (P > 0.05). No implant was observed for loss during the follow-up period of 8-12 months after crown placement. ConclusionsThe success of the pouch and non-pouch techniques in repairing extensive membrane perforation is evident in terms of clinical and radiographic outcomes. Regardless of whether the pouch or non-pouch technique is used, secure and stable implant restoration results can be achieved after the maxillary sinus membrane perforation is repaired.

Direct numerical simulation of fully-developed supersonic turbulent channel flows with dense vapors

This work aims to investigate the impact of the molecule-complexity effect and the non-ideal effect on wall-bounded turbulent flows by applying direct numerical simulation (DNS) to fully-developed channel flows of two typical organic vapors: R1233zd(E) and octamethyltrisiloxane (MDM). For each vapor, three thermodynamic states are analyzed: one in the dilute-gas region, one near the saturation line, and one in the supercritical region. For mean flow fields, it is found that, due to smaller Prandtl and Eckert numbers, both the molecule-complexity effect and the non-ideal effect reduce the mean temperature rise from the cold wall to the channel center. Meanwhile, the molecule-complexity effect weakens the mean density drop, while the non-ideal effect strengthens the drop. Furthermore, once the density and viscosity variations are considered, the mean streamwise velocity profiles of dense vapors are practically the same as the ideal gas. For turbulent fluctuations, it is found that the correlations between T′, p′, and ρ′ in dense vapors are more complicated than the ideal gas: for the ideal gas, fluctuations are dominated by “vorticity mode”; hence, ρ′ and T′ are strongly related to u′ but independent of p′; however, for dense vapors, “acoustic mode” can also play an important role. A newly derived equation illustrates that, through the “acoustic mode,” the molecule-complexity effect obviously enhances the positive correlation between ρ′ and p′, while the non-ideal effect can enhance the positive correlation between T′ and p′. Further analysis of instantaneous flow fields shows that p′ is isotropic. The isotropic character affects fluctuation magnitudes but has limited effect on the specified wall-direction turbulent transport. Consequently, Walz's equation and Reynolds analogy in terms of enthalpy are still valid. Finally, a comparison between the DNS energy budget and k equation of Reynolds-averaged Navier–Stokes (RANS) model has been carried out. Results show that obvious deviation happens on the production term in spite of the careful selection of eddy viscosity model.

Sediment Types with Alternation of Sandy and Rocky Shores Influence the Distribution of Clams in an Area Characterized by High-Energy Hydrodynamic Conditions

To identify short-term changes (14–69 days) in the adult abundance of two closely related shallow-burrowing bivalves (Ruditapes spp.), a series of observations and displacement assessments were made during the 2014–2018 period. This study was initiated to estimate the natural displacement of clams in a high-energy hydrodynamic tidal regime along the western coast of Cotentin in Normandy, France (western basin of the English Channel, northeastern Atlantic). The raking of several different surfaces and sediment types at successive periods separated by about one month shows clam displacement in most of the selected quadrats. The mean abundance displacement derived from all the observations carried out in 2014, 2016 and 2018 is estimated at 1.8 ind·m−2 mo−1, a value that should be compared with the mean density of between 2.0 and 12.5 ind·m−2 along the western coast of Cotentin. These displacements are confirmed with experiments using clams marked with an inox metal washer and detected with a Minelab Sovereign GT multi-frequency metal detector. During the course of the experiments, about 20% of the clams show a displacement. Most of the displacements are moderate, being less than 2 m, but some clams could be displaced by more than 20 m.

Poly(ether ketone ketone)/hollow silica filler substrates and application for sixth generation communication

AbstractThe influence of density and shape of hollow silicas (silica hollow tubes (SHT) or hollow glass microspheres (HGM)) on dielectric and heat‐resisting characteristics of poly(ether ketone ketone) (PEKK)/SHT and PEKK/HGM films was systematically investigated. The dielectric characteristics of PEKK/SHT or PEKK/HGM films decrease to a minimum, as their SHT or HGM contents approach 8 or 20 wt%, respectively, and the minimum dielectric constant (εr) and dielectric loss (tan δ) of PEKK/SHT or PEKK/HGM films decrease visibly with decreasing SHT densities. By filling with 0.46 g cm−3 mean density of SHT or HGM fillers, the minimum εr and tan δ of PEKK/SHT films are somewhat smaller than those of PEKK/HGM films. The linear coefficient of thermal expansion (LCTE) values of PEKK/SHT or PEKK/HGM films reduce and their onset degradation temperature values increase gradually with increasing SHT and HGM contents. Low εr/tan δ (2.11/0.0022 and 2.2/0.0027 at 1 MHz), LCTE (35.5 × 10−6 and 31.2 × 10−6 °C−1) and excellent heat‐resisting properties favorable for sixth generation (6G) ultrarapid communication are realized for appropriately prepared PEKK/SHT and PEKK/HGM substrate films. The free‐volume‐hole characteristics of PEKK/HGM or PEKK/SHT films approach a maximum as SHT or HGM contents reach an optimum value of 8 or 20 wt%, respectively. © 2024 Society of Chemical Industry.

Trinitroglycerin-loaded chitosan nanogels accelerate angiogenesis in wound healing process

Trinitroglycerin (TNG) with remarkable angiogenic, antibacterial, and antioxidative activity is a promising candidate to govern wound healing capacity. However, its clinical administration is limited due to associated complications and NO short half-life. In the current study, TNG-loaded chitosan nanogels (TNG-Ngs) were examined in-vitro and in-vivo to gain insight into their clinical application. We prepared TNG-Ngs and characterized their physiochemical properties. The potential of TNG-Ngs was assessed using biocompatibility, scratch assay, and a full-thickness skin wounds model, followed by histopathological and immunohistochemistry examinations. TNG-Ngs particle size 96 ± 18 and definite size distribution histogram. The loading capacity (LC) and encapsulation efficiency (EE) of prepared TNG-Ngs were 70.2 % and 2.1 %, respectively. The TNG-Ngs samples showed enhanced migration of HUVECs with no apparent cytotoxicity. The topical use of TNG-Ngs200 on the wounds revealed a complete wound closure ratio, skin component formation, less scar width, remarkable granulation tissue, promoted collagen deposition, and enhanced the relative mean density of α-SMA and CD31. TNG-Ngs accelerated wound healing by promoting collagen deposition and angiogenic activity, as well as reducing inflammation. The findings indicated that TNG-Ngs is expected to be well vascularized in the wound area and to be more effective in topical therapy.

Dormancy of a specialist herbivore, Anthonomus rufipennis (Coleoptera: Curculionidae), in a dry tropical forest.

The life history aspects of dormancy of the weevil Anthonomus rufipennis LeConte (Coleoptera: Curculionidae) were studied a 57-month period in a seasonally dry tropical forest of central Mexico. Weevil populations and their physiological status were monitored on both the reproductive host tree, Senna polyantha (Collad.) H.S: Irwin & Barneby (Fabales: Fabaceae) and the highly favored refuge host, Tillandsia recurvata L. (Poales: Bromeliaceae) or "ball moss." During the dry season, weevils were only found on the refuge host with a mean total density of 1.014 ± 2.532 individuals/ball moss (N = 1,681). Weevil densities on T. recurvata between early and late dry seasons were not significantly different, suggesting that dry season survival was relatively high. Weevils collected during these seasons revealed little reproductive development and relatively high-fat accumulation in both sexes. During 5 of 6 yr, densities of the weevils in T. recurvata dropped significantly during the early rainy seasons, when the reproductive host trees leafed out and began producing oviposition sites (flower buds). At this time, more males than females initially moved to vegetative trees and showed significant signs of reproductive development. Recolonization of ball moss by weevils began during the late rainy season when oviposition sites (flower buds) were still available. A proportion of the weevils remained on the reproductive host, suggesting that A. rufipennis is facultatively multivoltine. The methodologies and results of the study can serve as a model system for future studies of the dormancy of other insects in dry tropical forests and provide insight into the dormancy of other anthonomine weevils of economic importance.

Understanding the effect of Prandtl number on momentum and scalar mixing rates in neutral and stably stratified flows using gradient field dynamics

Recently, direct numerical simulations (DNS) of stably stratified turbulence have shown that as the Prandtl number ( $Pr$ ) is increased from 1 to 7, the mean turbulent potential energy dissipation rate (TPE-DR) drops dramatically, while the mean turbulent kinetic energy dissipation rate (TKE-DR) increases significantly. Through an analysis of the equations governing the fluctuating velocity and density gradients we provide a mechanistic explanation for this surprising behaviour and test the predictions using DNS. We show that the mean density gradient gives rise to a mechanism that opposes the production of fluctuating density gradients, and this is connected to the emergence of ramp cliffs. The same term appears in the velocity gradient equation but with the opposite sign, and is the contribution from buoyancy. This term is ultimately the reason why the TPE-DR reduces while the TKE-DR increases with increasing $Pr$ . Our analysis also predicts that the effects of buoyancy on the smallest scales of the flow become stronger as $Pr$ is increased, and this is confirmed by our DNS data. A consequence of this is that the standard buoyancy Reynolds number does not correctly estimate the impact of buoyancy at the smallest scales when $Pr$ deviates from 1, and we derive a suitable alternative parameter. Finally, an analysis of the filtered gradient equations reveals that the mean density gradient term changes sign at sufficiently large scales, such that buoyancy acts as a source for velocity gradients at small scales, but as a sink at large scales.

Deep-learning based analysis of in-vivo confocal microscopy images of the subbasal corneal nerve plexus’ inferior whorl in patients with neuropathic corneal pain and dry eye disease

PurposeTo evaluate and compare subbasal corneal nerve parameters of the inferior whorl in patients with dry eye disease (DED), neuropathic corneal pain (NCP), and controls using a novel deep-learning-based algorithm to analyze in-vivo confocal microscopy (IVCM) images. MethodsSubbasal nerve plexus (SNP) images of the inferior whorl of patients with DED (n = 49, 77 eyes), NCP (n = 14, 24 eyes), and controls (n = 41, 59 eyes) were taken with IVCM and further analyzed using an open-source artificial intelligence (AI)-based algorithm previously developed by our group. This algorithm automatically segments nerves, immune cells, and neuromas in the SNP. The following parameters were compared between groups: nerve area density, average nerve thickness, average nerve segment tortuosity, junction point density, neuroma density, and immune cell density. Results160 eyes of 104 patients (63 % females), aged 56.8 ± 15.4 years, were included. The mean nerve area density was significantly lower in the DED (P = 0.012) and NCP (P < 0.001) groups compared to the control group. The junction point density was lower in the NCP group compared with control (P = 0.001) and DED (P = 0.004) groups. The immune cell density was higher in the DED group compared with controls (P < 0.001). ConclusionsDeep-learning-based analysis of IVCM images of the corneal SNP inferior whorl distinguished a decreased mean nerve area density in patients with DED and NCP compared with controls and an increased immune cell density in patients with oGVHD- and SS-associated DED. These findings suggest that the inferior whorl could be used as landmark to distinguish between patients with DED and NCP.

Statistics and Models of the Electron Plasma Density From the Van Allen Probes

AbstractWe use the full NASA Van Allen Probes mission (2012–2019) to extract the electron plasma density from the Electric and Magnetic Field Instrument Suite and Integrated Science (EMFISIS) and Electric Field and Waves (EFW) instruments and discuss the evolution of the plasmasphere. We generate new statistics including mean and standard deviations of the plasma density with respect to L‐shell, magnetic local time (MLT), and various geomagnetic indices. These statistics are generated to be applied in radiation belt physics and space weather codes (with fits provided). The mean plasmasphere is circular around Earth with respect to MLT for Kp &lt; 1. The mean 100 cm−3 level line is above L = 5 and mean 10 cm−3 level expands above the Van Allen Probes apogee for Kp &lt; 1. The outer electron belt lies within the plasmasphere for 60% of all times. As activity increases (Kp &gt; 2), a gradual MLT asymmetry forms with higher mean density in the afternoon sector due to plumes expanding outward. Conversely, the mean density decreases on the dawn and night sectors. The mean density is between ∼500 and ∼50 cm−3 between L ∼ 4 and L ∼ 6 during quiet and moderately active times (Kp &lt; 3), representing ∼80% of all times. Statistics in regions of high density below L = 2 are underdefined for intense activity. The highest standard deviation of density represents a factor 2.5 to 3 times the mean above L = 5 and for active times. We find the percent difference between the EFW and EMFISIS densities is bounded by ±20% for quiet and moderate activity (Kp &lt; 5) and goes up to ±100% for extreme activity.

A phase 2a study investigating the effects of ritlecitinib on brainstem auditory evoked potentials and intraepidermal nerve fiber histology in adults with alopecia areata.

Reversible axonal swelling and brainstem auditory evoked potential (BAEP) changes were observed in standard chronic (9-month) toxicology studies in dogs treated with ritlecitinib, an oral Janus kinase 3/tyrosine kinase expressed in hepatocellular carcinoma family kinase inhibitor, at exposures higher than the approved 50-mg human dose. To evaluate the clinical relevance of the dog toxicity finding, this phase 2a, double-blind study assessed BAEP changes and intraepidermal nerve fiber (IENF) histology in adults with alopecia areata treated with ritlecitinib. Patients were randomized to receive oral ritlecitinib 50 mg once daily (QD) with a 4-week loading dose of 200 mg QD or placebo for 9 months (placebo-controlled phase); they then entered the active-therapy extension and received ritlecitinib 50 mg QD (with a 4-week loading dose of 200 mg in patients switching from placebo). Among the 71 patients, no notable mean differences in change from baseline (CFB) in Waves I-V interwave latency (primary outcome) or Wave V amplitude on BAEP at a stimulus intensity of 80 dB nHL were observed in the ritlecitinib or placebo group at Month 9, with no notable differences in interwave latency or Wave V amplitude between groups. The CFB in mean or median IENF density and in percentage of IENFs with axonal swellings was minimal and similar between groups at Month 9. Ritlecitinib treatment was also not associated with an imbalanced incidence of neurological and audiological adverse events. These results provide evidence that the BAEP and axonal swelling finding in dogs are not clinically relevant in humans.

Ashwagandha's Multifaceted Effects on Human Health: Impact on Vascular Endothelium, Inflammation, Lipid Metabolism, and Cardiovascular Outcomes-A Review.

Withania somnifera, commonly known as Ashwagandha, has been popular for many years. Numerous studies have shown that the extract of this plant, due to its wealth of active substances, can induce anti-inflammatory, neuroprotective, immunomodulatory, hepatoprotective, cardioprotective, anti-diabetic, adaptogenic, anti-arthritic, anti-stress, and antimicrobial effects. This review examines the impact of Ashwagandha extract on the vascular endothelium, inflammation, lipid metabolism, and cardiovascular outcomes. Studies have shown that Ashwagandha extracts exhibit an anti-angiogenic effect by inhibiting vascular endothelial growth factor (VEGF)-induced capillary sprouting and formation by lowering the mean density of microvessels. Furthermore, the results of numerous studies highlight the anti-inflammatory role of Ashwagandha extract, as the action of this plant causes a decrease in the expression of pro-inflammatory cytokines. Interestingly, withanolides, present in Ashwagandha root, have shown the ability to inhibit the differentiation of preadipocytes into adipocytes. Research results have also proved that W. somnifera demonstrates cardioprotective effects due to its antioxidant properties and reduces ischemia/reperfusion-induced apoptosis. It seems that this plant can be successfully used as a potential treatment for several conditions, mainly those with increased inflammation. More research is needed to elucidate the exact mechanisms by which the substances contained in W. somnifera extracts can act in the human body.

A low-mass sub-Neptune planet transiting the bright active star HD 73344

Context. Planets with radii of between 2 and 4 R⊕ closely orbiting solar-type stars are of significant importance for studying the transition from rocky to giant planets, and are prime targets for atmospheric characterization by missions such as JWST and ARIEL. Unfortunately, only a handful of examples with precise mass measurements are known to orbit bright stars. Aims. Our goal is to determine the mass of a transiting planet around the very bright F6 star HD 73344 (Vmag = 6.9). This star exhibits high activity and has a rotation period that is close to the orbital period of the planet (Pb = 15.6 days). Methods. The transiting planet, initially a K2 candidate, is confirmed through TESS observations (TOI 5140.01). We refined its parameters using TESS data and rule out a false positive with Spitzer observations. We analyzed high-precision radial velocity (RV) data from the SOPHIE and HIRES spectrographs. We conducted separate and joint analyses of K2, TESS, SOPHIE, and HIRES data using the PASTIS software. Given the star’s early type and high activity, we used a novel observing strategy, targeting the star at high cadence for two consecutive nights with SOPHIE to understand the short-term stellar variability. We modeled stellar noise with two Gaussian processes: one for rotationally modulated stellar processes, and one for short-term stellar variability. Results. High-cadence RV observations provide better constraints on stellar variability and precise orbital parameters for the transiting planet: a radius of Rb = 2.88−0.07+0.08 R⊕ and a mass of Mb = 2.98−1.90+2.50 M⊕ (upper-limit at 3σ is &lt;10.48 M⊕). The derived mean density suggests a sub-Neptune-type composition, but uncertainties in the planet’s mass prevent a detailed characterization. In addition, we find a periodic signal in the RV data that we attribute to the signature of a nontransiting exoplanet, without totally excluding the possibility of a nonplanetary origin. This planetary candidate would have a minimum mass of about Mc sin ic = 116.3 ± −13.0+12.8 M⊕ and a period of Pc = 66.45−0.25+0.10 days. Dynamical analyses confirm the stability of the two-planet system and provide constraints on the inclination of the candidate planet; these findings favor a near-coplanar system. Conclusions. While the transiting planet orbits the bright star at a short period, stellar activity prevented us from precise mass measurements despite intensive RV follow-up. Long-term RV tracking of this planet could improve this measurement, as well as our understanding of the activity of the host star. The latter will be essential if we are to characterize the atmosphere of planets around F-type stars using transmission spectroscopy.

Fungal trait‐environment relationships in wood‐inhabiting communities of boreal forest patches

Abstract Fungal traits can provide a mechanistic understanding of how wood‐inhabiting fungi interact with their environment and how that influences community assembly in deadwood. However, fungal trait exploration is relatively new and almost no studies measure fungal traits in their environment. In this study we tested species‐ and trait‐environment relationships in reproducing fungal communities inhabiting 571 Norway spruce (Picea abies) logs in 55 isolated forest patches (0.1–9.9 ha) of different naturalness types, located in Northern boreal Sweden. The studied patches were (1) semi‐natural set‐aside patches within highly managed landscapes, or (2) old‐growth natural patches located in an unmanaged landscape. We tested species and trait relationships to deadwood substrate and forest patch variables. We measured mean fruit body size and density for each of the 19 species within communities. Traits assembled in relation to log decay stage and forest patch naturalness, illustrating the important role of deterministic environmental filtering in shaping reproducing wood‐inhabiting fungal communities. Early decay stage communities had larger, less dense, annual fruiting bodies of half‐resupinate type and were more often white‐rot fungi. Species rich mid‐decay stage communities had mixed trait assemblages with more long lived perennial fruit bodies of intermediate size, and both brown‐ and white‐rot fungi equally represented. Finally, late decay stage communities had smaller, denser and perennial fruit bodies, more often of the brown‐rot type. The relationships between the studied traits and decay stages were similar in both set‐aside and natural patches. However, set‐aside semi‐natural patches in highly managed landscapes more frequently supported species with smaller, perennial and resupinate fruit bodies compared to natural patches in an unmanaged landscape. Synthesis. We found that log decay stage was the primary driver of fungal community assembly of species and traits in isolated forest patches. Our results suggest that decay stage filters four reproduction traits (fruit body density, size, lifespan and type) and one resource‐use trait (white or brown rot). Our results highlights, for the first time, that communities with diverse fungal reproductive traits are maintained foremost across all deadwood decay stages under different forest naturalness conditions. Read the free Plain Language Summary for this article on the Journal blog.

Long-Term Surgical Outcomes of Scleral Flap versus Scleral Pocket Technique for Sutureless Intrascleral One-Piece Lens Fixation.

Objectives: This study compared long-term surgical outcomes of the scleral flap versus scleral pocket technique for sutureless intrascleral one-piece intraocular lens (IOL) fixation. Methods: A retrospective comparative study was conducted at a single center, involving consecutive patients undergoing sutureless intrascleral one-piece IOL implantation, between January 2020 and May 2022. Eyes were divided into two groups based on the surgical technique: group 1 underwent scleral flap (n = 64), and group 2 received scleral pocket technique (n = 59). Visual acuity, refractive outcomes, and complications were assessed over a minimum 24-month follow-up period. Results: Both groups showed improvements in best-corrected visual acuity (BCVA), increasing from 0.84 ± 0.56 logMAR at baseline to 0.39 ± 0.23 logMAR (p = 0.042) at 24 months in group 1 and from 0.91 ± 0.63 logMAR at baseline to 0.45 ± 0.38 logMAR (p = 0.039) at 24 months in group 2. No significant differences in BCVA were observed between the groups at baseline (p = 0.991), 12 (p = 0.496) and 24 months (p = 0.557). Mean spherical equivalent (-0.73 ± 1.32 D in group 1 and -0.92 ± 0.99 D in group 2, p = 0.447), refractive prediction error (-0.21 ± 1.1 D in group 1 and -0.35 ± 1.8 D in group 2, p = 0.377), and surgically induced astigmatism (0.74 ± 0.89 D in group 1 and 0.85 ± 0.76 in group 2, p = 0.651) were comparable between the two groups. An IOL tilt of 5.5 ± 1.8 and 5.8 ± 2.0 degrees (p = 0.867) and an IOL decentration of 0.41 ± 0.21 mm and 0.29 ± 0.11 mm (p = 0.955) were obtained, respectively, in group 1 and group 2 at 24 months. Mean endothelial cell density remained stable at 24 months in both groups (p = 0.832 in group 1 and p = 0.443 in group 2), and it was 1747.20 ± 588.03 cells/mm2 in group 1 and 1883.71 ± 621.29 cells/mm2 in group 2 (p = 0.327) at baseline, 1545.36 ± 442.3 cells/mm2 in group 1 and 1417.44 ± 623.40 cells/mm2 in group 2 (p = 0.483) at 24 months. No cases of endophthalmitis were observed. Conclusions: The scleral pocket technique for sutureless intrascleral one-piece IOL fixation is comparable to the traditional scleral flap technique in terms of long-term visual outcomes and safety. The scleral pocket technique offers a simplified approach and a viable option even for less experienced surgeons.

Combined use of niraparib enhanced the inhibitory effect of Anti-GD2 antibody on osteosarcoma cells

PurposeThis study aims to investigate the effect of Niraparib in combination with an Anti-GD2 Antibody on osteosarcoma cells.MethodsScratch test was utilized to assess cell migration capacity, while the Transwell experiment was utilized to evaluate cell invasion potential. Cell proliferation was measured using the CCK8 experiment. The affinity between the anti-GD2 antibody and its antigen was determined via ELISA. Tumor growth was evaluated through animal experiments. Western blotting, QRT-PCR, and histological analysis were conducted to examine the expression of relevant proteins and mRNAs.ResultsMG63 cell line was used for an example. The scratch test showed that the migration rate of osteosarcoma cells in Niraparib + Anti-GD2 group was 1.07 ± 0.04 after 48 h, and 0.34 ± 0.04 in the Control group. Transwell experiment showed that the invasion ability of osteosarcoma cells in Niraparib + Anti-GD2 group was 21.0 ± 1.5, and that in Control group was 87.7 ± 2.9. CCK8 experiment showed that the absorbance value of Niraparib + Anti-GD2 group was 0.16 ± 0.10 on day 5, and that of the Control group was 0.76 ± 0.09. Western blotting showed that the expression levels of BALP and CICP in Niraparib + Anti-GD2 group were 0.751 ± 0.135 and 1.086 ± 0.115, respectively, and those in Control group were 1.025 ± 0.143 and 1.216 ± 0.168, respectively. QRT-PCR results showed that the absorbance values of Niraparib + Anti-GD2 group were 0.173 ± 0.065 and 0.170 ± 0.078 on day 14. The results of animal experiments showed that on day 5, the tumor volume of the Control group was 2433 ± 391, and that of the Niraparib + Anti-GD2 group was 1137 ± 148. Histological analysis showed that the mean density values of Niraparib + Anti-GD2 group were 0.19 ± 0.08 and 0.22 ± 0.07, and those of Control group were 0.26 ± 0.09 and 0.29 ± 0.10.ConclusionThe combination of Niraparib and Anti-GD2 antibody significantly inhibits Osteosarcoma cells.

A 260 pc resolution ALMA map of HCN(1-0) in the galaxy NGC 4321

The property of star formation rate (SFR) is tightly connected to the amount of dense gas in molecular clouds. However, it is not fully understood how the relationship between dense molecular gas and star formation varies within galaxies and in different morphological environments. Most previous studies have typically been limited to kiloparsec-scale resolution such that different environments could not be resolved. In this work, we present new ALMA observations of HCN(1$-$0) at 260\,pc scale to test how the amount of dense gas and its ability to form stars varies with environmental properties. Combined with existing CO(2$-$1) observations from ALMA and from MUSE, we measured the HCN/CO line ratio, a proxy for the dense gas fraction, and SFR/HCN, a proxy for the star formation efficiency of the dense gas. We find a systematic $&gt; dex $ increase (decreases) of HCN/CO (SFR/HCN) towards the centre of the galaxy, and roughly flat trends of these ratios (average variations $&lt; dex $) throughout the disc. While spiral arms, interarm regions, and bar ends show similar HCN/CO and SFR/HCN, on the bar, there is a significantly lower SFR/HCN at a similar HCN/CO. The strong environmental influence on dense gas and star formation in the centre of NGC\,4321, suggests either that clouds couple strongly to the surrounding pressure or that HCN emission traces more of the bulk molecular gas that is less efficiently converted into stars. Across the disc, where the ISM pressure is typically low, SFR/HCN is more constant, indicating a decoupling of the clouds from their surrounding environment. The low SFR/HCN on the bar suggests that gas dynamics (e.g. shear and streaming motions) can have a large effect on the efficiency with which dense gas is converted into stars. In addition, we show that HCN/CO is a good predictor of the mean molecular gas surface density at 260\,pc scales across environments and physical conditions.

Factors affecting lymph node yield and density in neck dissection

Background Studies suggest that neck dissections with a minimum of 16–18 yielded nodes are associated with better overall survival compared to neck dissections with lower yields. Aims We aimed to identify factors affecting the lymph node yield and density in patients with oral cavity cancer undergoing elective neck dissection levels 1–3. Materials and methods Using prospectively registered data, we conducted a population-based cohort study on all patients surgically treated for oral cavity cancer including levels 1–3 neck dissection at our institution from 2018 to 2022. Uni and multivariate analyses were performed to identify factors associated with lymph node yields. Results In total, 221 patients were included. The mean lymph nodes yield and density were 19 (95%CI 18–20) and 0.12 (95%CI 0.09–0.16), respectively. In multivariate analysis, increasing body weight (p = .034) was positively and previous radiotherapy (p = .006) were negatively correlated with the number of yielded lymph nodes. Lymph node density was positively correlated with body weight (p = .011) and body mass index (p = .032) in univariate analysis. Conclusions and significance Increasing body weight was positively and previous radiotherapy was negatively correlated to lymph node yield. These factors should be taken into consideration when interpreting the lymph node yield as an indicator of neck dissection quality.

Biodiversity and functioning of mesozooplankton in a changing Ross Sea

Literature on Ross Sea zooplankton is limited, although it is the most productive system and has the highest biomass of phytoplankton in the Southern Ocean. Energy transfer within the food web and response of continental shelf food web to climate change depend on the knowledge of the density and distribution of zooplankton. We evaluated its density, composition, spatial distribution and their potential relations with environmental factors and specific water mass presence. Particular attention was given to copepods. Mesozooplankton samples were collected between 14 January and February 11, 2017 from 14 stations in the western Ross Sea and Terra Nova Bay, while other micronekton samplings were completed at 6 stations. Results highlighted three pools of stations: one inside Terra Nova Bay with the highest densities, one on the platform with lower mean density, and the third represented by a N–S transept at 175°E with the lowest mean density. This partitioning of the region fit with the grouping of stations according to a ternary plot based on the different percentages of water masses in each station and reflected the similarity of zooplankton. The presence of specific water masses and their contribution in the upper 200 m influences zooplankton biodiversity and density. A phylogenetic tree of the zooplankton was constructed to analyse the distance among the observed taxa. The area located near the coast exhibited the highest degree of phylogenetic overdispersion. In coastal waters, typical copepod species constituted the main part of the neritic zooplankton of Terra Nova Bay and Ross Sea. Oithona similis was the most abundant species both in coastal and offshore sectors, followed by pteropods, polychaetes and euphausiids.

Enhancing the accuracy and efficiency of Pacific walrus (Odobenus rosmarus divergens) surveys: A comparison of visual and aerial imagery-based counts at coastal haulouts.

This study presents a semi-automated approach utilizing unoccupied aerial vehicle (UAV) surveys to accurately estimate the abundance of Pacific walruses at large coastal haulouts in Chukotka, Russia. Seven major haulout sites were surveyed during the summers and falls of 2017-2019. Walrus counts were performed using three distinct methods: traditional visual land-based counts, complete head counts utilizing georeferenced UAV imagery, and counting walruses within model polygons within the haulout outline and employing various extrapolation techniques to predict walrus abundance across the haulout area. The results indicated that traditional visual counts neither yielded consistent results nor allowed for uncertainty estimation, unlike the site- and date-specific direct extrapolation method and the non-specific linear regression model. These latter methods consistently provided estimates, on average, within 5% of the "true" abundance determined through complete photo-based head counts. Beside yielding accurate estimates, these semi-automated methods significantly reduced counting time by at least 63%, in contrast to complete head counts. The non-specific model, which allowed the estimation of walrus abundance based on the type of the terrain and the haulout area was less accurate compared with site and date specific estimates, but provided a tool to estimate abundance when no field visits are conducted, e.g., by using high-resolution satellite imagery to measure haulout area. This model revealed that the haulouts located on flat sandy beaches exhibited mean walrus densities approximately 30.5% times higher than those on rocky shores surrounded by cliffs: 0.879 (SD = 0.1302) and 0.648 (SD = 0.1753) walrus per m2 correspondingly. The estimated daily walrus abundance at major Chukotkan haulouts in 2017-2019 ranged between 15 and 94,660 (mean = 10,397, SD = 14,477) walruses with the maximum seasonal abundances reported at Cape Serdtse-Kamen as 94,960 on 10-Oct-2017, 26,850 on 10-Oct-2018, and 87,595 on 10-Oct-2019.