Human Cancer Cell Line MCF-7 Research Articles

Au-TiO2NANOSPHERES CATALYZED ONE-POT-MCR SYNTHESIS, CHARACTERIZATION AND ANTIPROLIFERATION ACTIVITY OF INDOLYL-IMIDAZOLOPYRIDINES CARBOXYLIC ACID HYBRIDS

In this context, we describe the efficiency of this catalyst towards green organic synthesis has been studied by carrying out a mixture of 1-fluoro-4-isocyanobenzene (1), 6-aminonicotinic acid (2)and substituted 1H-indole-3- carbaldehyde (3a-i) in distilled water, porous Au/TiO2nanospheres were added at room temperature in H2O. It proceeds yielding the corresponding isubstituted-3-((4-fluorophenyl) amino)-2-(1H-indol-3-yl)imidazo [1,2- a]pyridine-6-carboxylic acid derivative in good to excellent yield viz one-pot multi-component reaction. All the title compounds were also screened for Anti- proliferation activity screened for their in vitro anticancer activity against four human cancer cell lines MCF-7, HeLa, HEK 293T and IMR 32. Among them 4a (having 4-OCH3 substituent) has shown promising activity with IC50 (µM) 16.74±1.86 (MCF-7), 40.68 ± 1.25(HeLa), 50.61±4.64 (HEK 293T) and 26.58±1.68 (IMR32) has shown IC50 (µM) and these results are close to that of standard drug Doxorubicin.

Anticancer activity of tin oxide and cerium-doped tin oxide nanoparticles synthesized from Ipomoea carnea flower extract

Background: The aim of the study is to investigate the anticancer potential of tin oxide (SnO2) and different concentrations (2%, 4%, 6%, and 8%) of cerium-doped tin oxide nanoparticles (Ce-SnO2 NPs) using Ipomoea carnea flower extract. The synthesized SnO2 and different concentrations (2%, 4%, 6%, and 8%) of Ce-SnO2 NPs was tested using a colorimetric-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay against MCF-7 human breast cancer cell line cells. Methods: Collection and preparation of plant extract is preliminarily carried out followed by the synthesis of Undoped and Cerium doped Tin oxide nanoparticles is achieved by standard protocol along with that its anticancer activity also studied in this research. Results: The anticancer activity increased in direct proportion to the cerium-dopant concentration. Experimental results demonstrated that 8% Ce-SnO2 NPs exhibited a potential anticancer effect compared with SnO2 and other concentrations of Ce-SnO2 NPs. Conclusion: According to the current findings, large-scale manufacturing of Ce-SnO2 NPs might be recommended to have effective anticancer agents against breast cancer cell lines.

In vitro cytotoxicity assay of Fucoidan extracted from Turbinaria conoides against cancer cell lines MCF7, A549, and normal cell line L929

The main aim of the study is to quantify the cytotoxic property of the Fucoidan extracted from the Turbinaria conoides using the MTT assay with the standard fucose. Fucoidan was extracted using the soaked water method and it was determined using the HPLC procedure the obtained Test sample Fucoidan extracted from the Turbinaria conoides and standard fucose was subjected to the cytotoxicity assay against the MCF7 Human breast cancer cell line, A549 lung cancer cell line, and L929 normal mouse fibroblast cell line. From the results it was found that the Test sample showed good IC50 value for MCF7 cell line then A549 with an increasing concentration 24 hours incubation at 37°C The IC50 for MCF7 was 115.21 µg/ml and A549 396.46µg/ml and the Fucoidan extract was checked for its cytotoxicity against the normal mouse fibroblast cell line L929, Fucoidan was found non-lethal to the L929 mouse fibroblast normal cell line. Standard fucose also gave a significant result towards MCF7 and against the L929. This indicates that the Fucoidan extracted from Tubinaria conoides shows better anticancer potential in it. Hence its application can be further extended in the pharmacological fields.

Chemical synthesis, spectral characterization and biological activities of new diphenylsulphone derived Schiff base ligand and their Ni(II) complexes

This work presents the preparation and spectral characterization of five diphenylsulphone derived Schiff base ligands (L1–L5). Using the aforementioned ligands, Ni(II) complexes were synthesized in 1:1 stoichiometric ratio. The synthesized ligands and their complexes were characterized by elemental analysis, 1H NMR, UV–Visible, FT-IR, ESI-MS, TGA analysis and magnetic susceptibility measurements.The results from the above analytical techniques revealed that the complexes are in an octahedral geometry. The antimicrobial activity of the synthesized Schiff base ligands and their metal complexes under study was carried out by using the agar well diffusion method. Further, the anticancer properties of the synthesized compounds are performed against MCF-7 cell line and human lung cancer cell line A-549 using Adriamycin as standard drug. The biological potency of the metal complexes were significant than their respective ligands.

GREEN SYNTHESIS AND EVALUATION OF In-vitro ANTICANCER (MCF-7) AND MOLECULAR DOCKING STUDIES OF V2+ AND CO2+ COMPLEXES OF SCHIFF BASE

New V2+and Co2+complexes of Schiff base derived from the isonicotinicacidhydrazide (INH) and 4-isopropyl benzaldehyde by Eco-friendly synthetic method. Present synthesis complies with the principle of Green Chemistry. The synthesized compound was structurally characterized by elemental analysis, metal estimation, magnetic moment, molar conductance, UV-visible, IR & Far-IR, and fluorescence emission spectra. Elemental analysis of Schiff base and its complexes were carried out and the results indicate the percentage of elements (C, H, N, and O) and stoichiometry of the complexes. The molar conductance of complexes was carried out and the result indicates the non-electrolytic nature of the complexes. The electrochemical properties of the Co2+ complex were carried out and confirmed a quasi-reversible one-electron transfer redox reaction. The magnetic moment measurement indicates that the complexes are paramagnetic. The octahedral geometry of vanadium and cobalt complexes, as well as the functional groups present in them, was determined using UV-vis and IR spectroscopy. The fluorescence spectra of the ligand and complexes show the fluorescence nature of the ligand. The DPPH free radical scavenging method was used to evaluate the antioxidant activity of Schiff base and its V2+ complex in vitro. The MTT assay was used to measure cytotoxicity, and the vanadium complex's in vitro anticancer effect on MCF-7 (human breast cancer) cell lines was studied. In breast cancer therapy, the human epidermal growth factor receptor 2 (HER2) is considered a validated target. In addition, the Agar disc diffusion model was used to evaluate the antibacterial activity of Staphylococcusaureus, Escherichia coli, and Pseudomonas aeruginous in vitro.

SYNTHESIS AND IN VITRO CYTOTOXIC EVALUATION OF NOVEL TRIAZOLE-BENZIMIDAZOLE EMBODIED PYRAZOLE DERIVATIVES AGAINST BREAST CANCER

A new series of novel triazole – benzimidazole containing pyrazole derivatives (10a-m) have been designed, synthesized, and characterized by their spectral data. These newly synthesized compounds (10a-m) were tested for their in vitro cytotoxic activity against MCF-7 human breast cancer cell lines. This shows that compound 10b proved to be the most prominent cytotoxic agent against MCF-7 cell lines.

Effect of biosynthesized silver nanoparticles by Garcinia mangostana extract against human breast cancer cell line MCF-7

Effect of biosynthesized silver nanoparticles by Garcinia mangostana extract against human breast cancer cell line MCF-7

Design and synthesis of novel imidazolidine-2,4-dione derivatives as InhA inhibitors: Spectral characterization, computational, and biological studies

Tuberculosis is a global disease caused by bacteria called Mycobacterium tuberculosis (M.tb) and is one of the top 10 causes of death from a single infectious agent. InhA (enoyl acylcarrier proteinreductase) from M.tb is the target that is involved in cell wall biosynthesis. The most effective drug to treat TB (tuberculosis) is INH (isoniazide) which is a prodrug, activated by KatG (catalase-peroxidaseenzyme) to generate isonicotinoyl radical that reacts with NAD (nicotinamide adenine dinucleotide) to form an INH-NADH complex, acting as a real InhA inhibitor. A series of novel imidazolidine-2,4-dione were designed by site identification and molecular docking using GLIDE. After synthesis, the compounds were characterized by 1H NMR, LC-MS, FT-IR, and elemental analysis. The compounds were found to be moderately active against M.tb. MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) assay was used to test the compounds for anticancer activity against human breast cancer cell line MCF-7.

Investigation of the Mechanism of hsa_circ_000 1429 Adsorbed miR-205 to Regulate KDM4A and Promote Breast Cancer Metastasis.

This study investigates the mechanism of hsa_circ_0001429 adsorbing miR-205 and regulating the expression of KDM4A to promote breast cancer metastasis and its mechanism. Mammary epithelial cells MCF-10A and human breast cancer cell lines BT474, SKBr-3, ZR-75-30, and MCF7 are cultured, and the mRNA expressions of hsa_circ_000 1429, miR-205, and KDM4A are detected by qRT-PCR; hsa_circ_000 1429 binds to miR-205, and miR-205 targets KDM4A. RIP verifies that hsa_circ_000 1429 binds to AGO2; RNA pull down results prove that hsa_circ_000 1429 binds to miR-205; MTT detects cell proliferation; transwell assay detects cell migration and invasion ability; flow cytometry detects cell apoptosis rate. The expressions of KDM4A, migration, and invasion-related factors, N-cadherin and MMP-9 protein, are detected by blot. hsa_circ_000 1429 may upregulate the KDM4A gene by adsorbing miR-205. Therefore, it will promote the proliferation, migration, and invasion of breast cancer cells and inhibit their apoptosis.

SYNTHESIS, ANTICANCER ACTIVITIES AND IN SILICO SCREENING OF 3-ACETYLCOUMARINHYDRAZONE SCAFFOLDS

3-acetylcoumarin hydrazone scaffolds, synthesized from 3-acetylcoumarin and substituted benzoic acid hydrazides are reported with structural characterization using IR, HRMS, 1H and 13C-NMR. The In vitro anticancer activities against three human cancer cell lines viz. MCF-7 (human breast cancer cell line), HeLa (human cervical cancer cell line) and SCC-40 (human oral squamous cell carcinoma) are carried out while the tumour selectivity of compounds are tested on the normal human peripheral blood mononuclear cells (PBMCs). The compounds 3ACOH, 3ACDH and 3ACMH exhibited higher sensitivity towards HeLa with GI50 values between 20.4 - 44.1μg/ml and this range of GI50 concentration of hydrazones showed no remarkable toxicity against normal PBMCs. Molecular docking studies revealed commendable binding interactions with cyclooxygenase enzyme (PDB ID 6COX). ADMET analysis shows the hydrazones are showing drug-likeness properties. The reported observations of 3-acetylcoumarin hydrazones suggest their possible role as promising new anticancer drug candidates.

Folate functionalized chitosan nanoparticles as targeted delivery systems for improved anticancer efficiency of cytarabine in MCF-7 human breast cancer cell lines

Anticancer drug cytarabine, has been widely used for treating haematological malignancies while it has minimal activity against solid tumours, which demands continuous infusion leading to high dose cytarabine toxicity. In this study, folate conjugated chitosan nanoparticles (FCCNP) were used for targeted delivery of cytarabine in breast adenocarcinoma cell lines by making use of the overexpressed folate receptors on the surface of MCF-7. Folate was conjugated to chitosan using carbodiimide. FCCNPs show spherical morphology with a size of<50 nm. Zeta potential of + 45.2 mV and PDI of 0.98 from DLS measurement confirms a stable monodisperse nanoformulation. Cytotoxicity was studied in folate receptor positive, MCF-7 and folate receptor negative, A-549 cell lines. Increased cellular uptake of the drug incorporated nanoparticles was confirmed in MCF-7 cells with fluorophore, squaraine 650 compared to A-549 cells. The relative fold of expression of genes involved in apoptosis such as bax, cyt c and cas 9 were upregulated. The present in vitro study confirms improved cytotoxicity of cytarabine folate conjugated chitosan nanoparticles in MCF-7 cells.

Structure Based Drug Discovery, Docking, Modelling, Synthesis and Anticancer Screening of Some Novel Quinoline Derivatives

A new series of (2-(substituted-phenyl) quinoline-4-yl) (3-(substituted phenyl)-5-phenyl-1H-pyrazol-1-yl) methanone derivatives was carried out docking modelling and synthesized. Purity was checked by TLC and chemical structures of synthesized compounds were elucidated by their IR, 1HNMR, MS analysis data. The synthesized compounds were screened for anticancer activity by using cell line MCF-7 (Human breast cancer cell line) correlate with docking modelling.

Three-dimensional prints from 3-dimensional cell culture aggregates of human cancer cell lines

Objectives: Three-dimensional (3D) printing has gained significance for human health-care applications in recent years. Some of these applications include obtaining models which mimic anatomical parts. One other parallel development in the biological research area is the development of 3D cell cultures. Such cultures are now becoming the material of choice for in vitro experiments, fast replacing the traditional adherent/monolayer 2D culture approaches. We present here, a method to obtain 3D prints of 3D aggregates of three human cancer cell lines. Such 3D prints can be useful models to understand solid tumor morphologies and also as effective teaching models. Materials and Methods: Photomicrographs of the 3D aggregates of the human cancer cell lines SiHa, MCF-7, and A549 (human cervical cancer, breast cancer, and non-small cell lung cancer cell lines, respectively) were obtained using inverted phase contrast microscopy. Conversion of normal jpeg images into 3D files was performed using the lithophane method and CAD files obtained. The CAD files thus generated were used to print the objects using the Stratasys Polyjet J750 3D Printer. Results: We could obtain 3D prints of SiHa, MCF-7, and A549 (human cervical cancer, breast cancer, and non-small cell lung cancer cell lines, respectively) 3D aggregates/spheroids. Conclusion: It is hoped that this approach will be useful for studying solid tumor morphologies in finer details. Furthermore, other benefits of such 3D prints would be in them being excellent models for teaching purposes.

Design, synthesis, biological screening and molecular docking studies of novel multifunctional 1,4-di (aryl/heteroaryl) substituted piperazine derivatives as potential antitubercular and antimicrobial agents

In this paper, two series of novel multifunctional 1, 4-di (aryl/heteroaryl) substituted piperazine derivatives (6a-d &7a-d) were synthesized, characterized, and evaluated for their antitubercular, antibacterial, and antifungal activities. A step-wise reduction, bromination and substitution reactions on various aldehydes resulted in alcohols (2a–d), bromides (3a–d), and titled novel compounds (6a–d &7a–d) in moderate to good yields (48–85%). The novel compounds were evaluated for their antitubercular and antimicrobial activities. Compound 7a exhibited promising antitubercular activity (MIC: 0.65 µg/mL) almost equal to the Rifampicin, while the rest of the compounds were moderately active against MTB H37Rv except 6b. Compounds 7a and 6b showed good activity against tested fungal pathogens. Compounds 7a and 7b were proven as the best bacterial agents. Molecular docking studies were in agreement with the in-vitro results. Docking analyses show that all the synthesized molecules bind to the target protein Mtb RNAP (PDB ID: 5UHC) fairly strongly. All the compounds were evaluated for their in vitro cytotoxicity effect using the MTT assay method against human cancer cell line MCF-7. The compounds demonstrated growth inhibitory effect on the cell line with significant IC50 values ranging between 8.20 and 34.45 µM. Most importantly, compound 7a displayed good binding affinity towards the tested protein with binding energy −7.30 kcal/mol and a stronger hydrogen bond distance of 2.2 Å with ASN-493 residue. Thus, the present research highlighted the potential role of novel piperazine derivatives as potential antitubercular, and antimicrobial candidates and further good research into optimization might result in the development of new antitubercular drug candidates.

Anlotinib inhibits the proliferation, migration and invasion, and induces apoptosis of breast cancer cells by downregulating TFAP2C.

The vascular endothelial growth factor receptor (VEGFR) network contributes to breast cancer pathogenesis and progression. Anlotinib is a highly potent multi-target tyrosine kinase inhibitor that has been previously shown to exert antitumor effects in various types of cancer. The aim of the present study is to investigate the effect of Anlotinib against breast cancer cells in vitro and uncover the possible underlying mechanisms. The human breast cancer cell line MCF-7 was treated with different concentrations of Anlotinib, before cell proliferation, migration, invasion and apoptosis were assessed using colony formation, wound healing, Transwell and TUNEL staining assays. In addition, the expression of transcription factor AP-2γ (TFAP2C) following Anlotinib stimulation was measured using reverse transcription-quantitative PCR and western blot analysis. TFAP2C was overexpressed in MCF-7 using transfection with a pcDNA3.1 vector, before the aforementioned experiments were repeated. The results revealed that Anlotinib impaired cell viability and colony formation, reduced proliferating cell nuclear antigen, Ki-67, MMP2, MMP9 and Bcl-2 expression levels, and inhibited cell migration and invasion. By contrast, the expression levels of tissue inhibitor of metalloproteinase 1, the frequency of apoptotic cells, the expression of Bax and the cleaved caspase-3/caspase-3 ratio increased in a concentration-dependent manner. Additionally, the expression of TFAP2C decreased after Anlotinib treatment. However, TFAP2C overexpression partially blocked the effects of Anlotinib on the proliferation, migration, invasion and apoptosis of MCF-7 cells. To conclude, Anlotinib suppressed proliferation, migration and invasion, whilst inducing apoptosis of MCF-7 cells, which may be partially dependent on the inhibition of TFAP2C expression.

Cytotoxic Activity, Apoptosis Induction and Cell Cycle Arrest in Human Breast Cancer (MCF7) Cells by a Novel Fluorinated Tetrahydro-[1,2,4]Triazolo[3,4-a]Isoquinolin Chalcones

A series of fluorinated chalcones (3a-f) was synthesized and confirmed by several spectral tools. The cytotoxic effect of this series was tested against a panel of different cancer cell lines (MCF7, A549, HCT116, and PC3). MTT assay revealed that chalcone 3f has the potent cytotoxic activity against all tested cancer cell lines except A549 cells. Chalcone 3f showed the least cytotoxic activity against the normal epithelial cell line RPE-1 and the lowest IC50 at 10.96 µM relative to the IC50 of doxorubicin at 12.8 µM against the human breast cancer cell line MCF7. Molecular docking studies showed a good interaction of chalcone 3f with the active site of histone demethylase (PLU-1/JARID1B) and Carboxy-terminal binding protein1 (CtBP1) proteins. Mechanistically, chalcone 3f induced cell cycle arrest at G2/M phase and apoptosis assessed by flow cytometry, as well as DNA fragmentation in MCF7 cells. Chalcone 3f upregulated mRNA expression levels of the apoptotic genes BAX, p53, and Caspase-7, Caspase-8, and Caspase-9, whereas mRNA expression levels of the antiapoptotic gene Bcl2, metastasis-related gene matrix metalloproteinase 1 (MMP1), and the autophagic markers ATG5 and LC3B were downregulated as quantified by qPCR. This study shows a cytotoxic effect of chalcone 3f against cancer cells and emerges as a promising therapeutic drug against breast cancer.

Naphthopyrone and anthraquinone derivatives from Comanthus delicata

• Fourteen pigments were isolated from the crinoid Comanthus delicata . • Five new napthopyrone derivatives, delicapyrons A − E ( 1 − 5 ), were elucidated. • 1 and 2 possess novel bisnaphthopyrone structures firstly described to date. • 7 significantly induced apoptosis in SK-Mel-2 cells and increased caspase 3 activity. Five new naphthopyrone derivatives, delicapyrons A − E ( 1 − 5 ), and nine known compounds were isolated from the MeOH extract of the Vietnamese crinoid Comanthus delicata . Their structures were elucidated by spectroscopic methods, including HRQTOFMS and 1D and 2D NMR experiments. The new compounds 1 and 2 possess novel bisnaphthopyrone structures containing linear and angular naphthopyrone units that were linked together via a methylene group and are first described herein. Among the isolated compounds, delicapyron B ( 2 ) exhibited significant and selective cytotoxicity against SK-Mel-2 and MCF7 human cancer cell lines, whereas delicapyron D ( 4 ), comaparvin ( 6 ), 6-methoxycomaparvin ( 7 ), and 5,8-dihydroxy-6,10-dimethoxy-2-methyl-4 H -naphtho[1,2- b ]pyran-4-one ( 8 ) showed this activity against the SK-Mel-2 cell line. In addition, compound 7 significantly induced cell death through apoptosis in SK-Mel-2 cells and increased caspase 3 activity at both 20 and 100 μM.

Synthesis, crystallography, DFT, MTT assay, and molecular docking studies of an exocyclic double-bonded crystalline chalcone

• Exocyclic double-bonded chalcone was synthesized by base-catalyzed Claisen-Schmidt reaction and crystallized using slow evaporation solution method. • C—C, C—O, C—H, H—H, and H—O interactions were identified from Hirshfeld surface analysis. • DFT/TD-DFT studies indicate π→π* transitions and intramolecular charge transfer. • The molecule shows promising anticancer activity against MCF-7 cancer cells. • Molecular docking studies indicate the inhibition of EGFR kinase protein via hydrogen bonding with methionine 769 amino acid residue. A bicyclic chalcone 2-(4-methoxybenzylidene)-3,4-dihydro- 2H -naphthalen-1-one (MDN) with an exocyclic double bond was synthesized using Claisen-Schmidt condensation reaction and subsequently crystallized through slow evaporation solution method. The newly synthesized chalcone was characterized using single-crystal X-ray diffraction, and FT-IR spectroscopy. The compound exhibits intermolecular interactions evident from Hirshfeld surface analysis. The bicyclic moiety has an IC 50 value of 0.59 mM against MCF-7 human breast cancer cell lines. The electronic structure of the compound, as obtained from DFT and TD-DFT calculations, reveals the presence of intramolecular charge transfer and π-π* electronic transitions. The in silico molecular docking study divulges the stabilizing interactions between the chalcone and EGFR receptor tyrosine kinase protein, namely hydrogen bonding and hydrophobic interactions. Overall, the synthesized chalcone could serve as a precursor for the functionalization of exocyclic double-bonded chalcone compounds.

Combined treatment with glucagon-like peptide-1 receptor agonist exendin-4 and metformin attenuates breast cancer growth.

Cancer is a major cause of death in patients with diabetes. Incretin therapy has received much attention because of its tissue-protective effects. We have previously reported an anti-breast cancer effect of glucagon-like peptide-1 receptor agonist exendin-4 (Ex-4). An anti-cancer effect of metformin is well recognized. Therefore, we examined the effect of combined treatment with Ex-4 and metformin in breast cancer cells. In human breast cancer cell lines MCF-7, MDA-MB-231, and KPL-1, 0.1-10mM metformin significantly reduced the cell number in growth curve analysis in a dose-dependent manner. Furthermore, combined treatment with 0.1mM metformin and 10nM Ex-4 additively attenuated the growth curve progression of breast cancer cells. In a bromodeoxyuridine (BrdU) assay, Ex-4 or metformin significantly decreased breast cancer cell proliferation and further reduction of BrdU incorporation was observed by combined treatment with Ex-4 and metformin, which suggested that Ex-4 and metformin additively decreased DNA synthesis in breast cancer cells. Although apoptotic cells were not observed among Ex-4-treated breast cancer cells, apoptotic cells were clearly detected among metformin-treated breast cancer cells by apoptosis assays. Furthermore, metformin decreased BCL-2 expression in MCF-7 cells. In vivo experiments using a xenograft model showed that Ex-4 and metformin significantly decreased the breast tumor weight and Ki67-positive proliferative cancer cells, and metformin reduced the serum insulin level in mice. These data suggested that Ex-4 and metformin attenuated cell proliferation and metformin induced apoptosis in breast cancer cells. Combined treatment of Ex-4 and metformin may be an optional therapy to inhibit breast cancer progression.

An In Vitro and In Silico Study of the Enhanced Antiproliferative and Pro-Oxidant Potential of Olea europaea L. cv. Arbosana Leaf Extract via Elastic Nanovesicles (Spanlastics).

The olive tree is a venerable Mediterranean plant and often used in traditional medicine. The main aim of the present study was to evaluate the effect of Olea europaea L. cv. Arbosana leaf extract (OLE) and its encapsulation within a spanlastic dosage form on the improvement of its pro-oxidant and antiproliferative activity against HepG-2, MCF-7, and Caco-2 human cancer cell lines. The LC-HRESIMS-assisted metabolomic profile of OLE putatively annotated 20 major metabolites and showed considerable in vitro antiproliferative activity against HepG-2, MCF-7, and Caco-2 cell lines with IC50 values of 9.2 ± 0.8, 7.1 ± 0.9, and 6.5 ± 0.7 µg/mL, respectively. The encapsulation of OLE within a (spanlastic) nanocarrier system, using a spraying method and Span 40 and Tween 80 (4:1 molar ratio), was successfully carried out (size 41 ± 2.4 nm, zeta potential 13.6 ± 2.5, and EE 61.43 ± 2.03%). OLE showed enhanced thermal stability, and an improved in vitro antiproliferative effect against HepG-2, MCF-7, and Caco-2 (IC50 3.6 ± 0.2, 2.3 ± 0.1, and 1.8 ± 0.1 µg/mL, respectively) in comparison to the unprocessed extract. Both preparations were found to exhibit pro-oxidant potential inside the cancer cells, through the potential inhibitory activity of OLE against glutathione reductase and superoxide dismutase (IC50 1.18 ± 0.12 and 2.33 ± 0.19 µg/mL, respectively). These inhibitory activities were proposed via a comprehensive in silico study to be linked to the presence of certain compounds in OLE. Consequently, we assume that formulating such a herbal extract within a suitable nanocarrier would be a promising improvement of its therapeutic potential.