GREEN SYNTHESIS AND EVALUATION OF In-vitro ANTICANCER (MCF-7) AND MOLECULAR DOCKING STUDIES OF V2+ AND CO2+ COMPLEXES OF SCHIFF BASE

Abstract

New V2+and Co2+complexes of Schiff base derived from the isonicotinicacidhydrazide (INH) and 4-isopropyl benzaldehyde by Eco-friendly synthetic method. Present synthesis complies with the principle of Green Chemistry. The synthesized compound was structurally characterized by elemental analysis, metal estimation, magnetic moment, molar conductance, UV-visible, IR & Far-IR, and fluorescence emission spectra. Elemental analysis of Schiff base and its complexes were carried out and the results indicate the percentage of elements (C, H, N, and O) and stoichiometry of the complexes. The molar conductance of complexes was carried out and the result indicates the non-electrolytic nature of the complexes. The electrochemical properties of the Co2+ complex were carried out and confirmed a quasi-reversible one-electron transfer redox reaction. The magnetic moment measurement indicates that the complexes are paramagnetic. The octahedral geometry of vanadium and cobalt complexes, as well as the functional groups present in them, was determined using UV-vis and IR spectroscopy. The fluorescence spectra of the ligand and complexes show the fluorescence nature of the ligand. The DPPH free radical scavenging method was used to evaluate the antioxidant activity of Schiff base and its V2+ complex in vitro. The MTT assay was used to measure cytotoxicity, and the vanadium complex's in vitro anticancer effect on MCF-7 (human breast cancer) cell lines was studied. In breast cancer therapy, the human epidermal growth factor receptor 2 (HER2) is considered a validated target. In addition, the Agar disc diffusion model was used to evaluate the antibacterial activity of Staphylococcusaureus, Escherichia coli, and Pseudomonas aeruginous in vitro.