An efficient polarization beam splitter (PBS) based on an optofluidic ring resonator (OFRR) is proposed and experimentally demonstrated. The PBS relies on the large effective refractive index difference between transverse-electric (TE) and transverse-magnetic (TM) polarization states, since the silica-microcapillary-based OFRR possesses a slab-like geometry configuration in the cross section through which the circulating light travels. To the best of our knowledge, this is the first OFRR-based PBS. In our work, the maximum polarization splitting ratio of up to 30 dB is achieved. Besides, water and ethanol are pumped into the core of the silica microcapillary respectively, and the maximum wavelength tuning range of 7.02 nm is realized when ethanol flows through the core, verifing the tuning principle of the PBS effectively. With such a good performance and simple scheme, this OFRR-based PBS is promising for applications such as tunable optical filters, demultiplexers, and routers.