Maximum Quantum Yield Of Photosystem Research Articles

The Combination of Increased Temperatures and High Irradiation Causes Changes in Photosynthetic Efficiency.

Global warming and the associated climate change are imposing abiotic stress on plants. Abiotic factors are crucial for plant productivity, survival, and reproduction. Eight sunflower hybrids were tested in conditions of different water availability and with combinations of different temperatures and irradiation. The changes in the photosynthetic efficiency were measured in the morning (control conditions: 2013, 25.8 °C and 349.1 W m−2; 2014, 21.8 °C and 296.4 W m−2) and afternoon (the combination of increased temperatures and high irradiation: 2013, 34 °C and 837.9 W m−2; 2014, 29.4 °C and 888.9 W m−2) at a flowering stage in rainfed or irrigated conditions. The measurement time (morning and afternoon conditions) had a statistically significant effect on all the tested parameters. The performance index (PIABS) in 2013 and the maximum quantum yield of photosystem II (TR0/ABS) in 2014 are the only parameters significantly affected by the irrigation. As a result of the combined effect of increased temperatures and high irradiation, PIABS values decreased by 73–92% in rainfed conditions and by 63–87% in irrigated conditions in 2013, depending on the hybrid, while in 2014, the decrease varied between 70 and 86%. The TR0/ABS decrease was 7–17% in 2013, depending on the hybrid, and 6–12% in 2014, both in rainfed and irrigated conditions. The principal component analysis confirmed the effect of the combination of increased temperatures and high irradiation on hybrids, sorting them exclusively according to the time of measurement. All investigated parameters highly fluctuated between hybrids but without observable trends for the morning and afternoon conditions, as well as for irrigation. Plants’ reaction to the combination of increased temperatures and high irradiation manifested as a change in their photosynthetic efficiency, i.e., the photosynthetic apparatus’ functioning was impaired.

A Link Between Plant Stress and Hydrodynamics? Indications From a Freshwater Macrophyte

AbstractLive plants are increasingly used in hydraulic laboratories to investigate flow‐vegetation interactions. In such experiments, they are often exposed to stressful handling and storage that can cause strong physiological responses and modifications in plant biomechanics. Little is known about the potential effect of these impacts on the performance of plants during hydraulic experiments. In this multidisciplinary study with a freshwater macrophyte (Potamogeton natans) we assess whether the duration and the conditions in which plants are stored in a laboratory prior to testing can impact plant stress, biomechanics and hydrodynamics, and quantify this impact. Plant stress was evaluated using chlorophyll fluorescence analysis (and the maximum quantum yield of photosystem II as specific indicator). Plant hydrodynamics were assessed using the drag coefficient calculated from drag force measurements at two flow scenarios. The results show that different plant handling/storage procedures can have a significant impact on plant hydrodynamics even within a short time frame, with a variation of the mean drag coefficient of approximately 30% across groups, which is comparable to the variation found across different species of freshwater macrophytes in previous studies. Plants with the highest level of stress were also characterized by the lowest drag coefficient across the groups considered, suggesting a potential link between plant stress and hydrodynamics.

Overexpression of 7-hydroxymethyl Chlorophyll a Reductase from Cucumber in Tobacco Accelerates Dark-Induced Chlorophyll Degradation.

7-hydroxymethyl chlorophyll (Chl) a reductase (HCAR) plays critical roles in the Chl cycle and degradation during leaf senescence, however, its function in horticultural crops remains unknown. Here, we identified an HCAR gene (CsHCAR) from cucumber (Cucumis sativus L.) and investigated its roles in response to dark-induced Chl degradation. CsHCAR encoded 459 amino acids, which were orthologous to Arabidopsis HCAR, had the conserved domains, and localized in the chloroplast. Gene expression analysis showed that CsHCAR expression was the highest in senescent leaves and was responsive to different stresses and phytohormone treatments. Overexpression of CsHCAR in tobacco accelerated dark-induced Chl degradation through enhancing the expression of Chl catabolic genes. After 10 d of darkness treatment, the biomass of CsHCAR overexpression plants was reduced. Furthermore, the value of net photosynthetic rate, maximum quantum yield of photosystem II, and effective quantum yield of photosystem II in CsHCAR overexpression plants was significantly reduced in comparison to that in wild-type (WT) plants. The photosynthetic protein content, including Lhcb1, Lhcb2, Lhcb4, RbcS, and RbcL in CsHCAR overexpression plants exhibited a lower level as compared to that observed in WT plants. In addition, the expression of genes encoding these proteins in CsHCAR overexpression plants was significantly lower than that in WT plants. Moreover, CsHCAR overexpression plants inhibited the dark-induced accumulation of reactive oxygen species (ROS). These results indicate that CsHCAR affects the stability of photosynthetic proteins in chloroplasts, positively regulates Chl degradation, and plays an important role in maintaining ROS homeostasis in leaves.

Response of physiological traits, antioxidant enzymes and nutrient uptake of soybean to Azotobacter Chroococcum and zinc sulfate under salinity

Globally, one of the key restrictions on agricultural production is salinity and its impact is expected to increase. As a result, it is necessary to improve crop salinity tolerance. One promising approach is the use of plant nutrition technologies such as the application of zinc sulfate (Zn) and Azotobacter chroococcum (Az) which may improve plant survival under environmental stresses. A greenhouse experiment was conducted with three replications and included four levels of salinity (0, 40, 60 and 80 mM NaCl) in combination with four treatments [control, 2 mM zinc sulfate foliar application (Zn), inoculation with Azotobacter chroococcum (Az) and co-application of Az and Zn (Az+Zn)]. The results indicate that exposure to salinity increased soybean contents of anthocyanin, malondialdehyde (MDA), osmolytes, sodium ion (Na+), and the activities of antioxidant enzymes However, the amount of chlorophyll and carotenoid, relative water content (RWC), maximum quantum yield of photosystem II (PSII; Fv/Fm), membrane stability index (MSI), and ion contents decreased. Application of Zn and inoculation with Az enhanced the chlorophyll (a, b and a+b), carotenoid, RWC, Fv/Fm, MSI, anthocyanin, osmolytes, N, K+, P contents, and K+/Na+; the activities of CAT, SOD, POX, and APX as well as MDA and Na+ contents were reduced. Overall our results reveal that combined Zn and Az application was the most effective treatment for alleviating the negative effects of salinity stress in soybean.

The Effect of Photoperiod on Necrosis Development, Photosynthetic Efficiency and 'Green Islands' Formation in Brassica juncea Infected with Alternaria brassicicola.

The main goal of growing plants under various photoperiods is to optimize photosynthesis for using the effect of day length that often acts on plants in combination with biotic and/or abiotic stresses. In this study, Brassica juncea plants were grown under four different day-length regimes, namely., 8 h day/16 h night, 12 h day/12 h night, 16 h day/8 h night, and continuous light, and were infected with a necrotrophic fungus Alternaria brassicicola. The development of necroses on B. juncea leaves was strongly influenced by leaf position and day length. The largest necroses were formed on plants grown under a 16 h day/8 h night photoperiod at 72 h post-inoculation (hpi). The implemented day-length regimes had a great impact on leaf morphology in response to A. brassicicola infection. They also influenced the chlorophyll and carotenoid contents and photosynthesis efficiency. Both the 1st (the oldest) and 3rd infected leaves showed significantly higher minimal fluorescence (F0) compared to the control leaves. Significantly lower values of other investigated chlorophyll a fluorescence parameters, e.g., maximum quantum yield of photosystem II (Fv/Fm) and non-photochemical quenching (NPQ), were observed in both infected leaves compared to the control, especially at 72 hpi. The oldest infected leaf, of approximately 30% of the B. juncea plants, grown under long-day and continuous light conditions showed a ‘green island’ phenotype in the form of a green ring surrounding an area of necrosis at 48 hpi. This phenomenon was also reflected in changes in the chloroplast’s ultrastructure and accelerated senescence (yellowing) in the form of expanding chlorosis. Further research should investigate the mechanism and physiological aspects of ‘green islands’ formation in this pathosystem.

Drought stress recovery of hydraulic and photochemical processes in Neotropical tree saplings.

Climate models predict an increase in the severity and the frequency of droughts. Tropical forests are among the ecosystems that could be highly impacted by these droughts. Here, we explore how hydraulic and photochemical processes respond to drought stress and re-watering. We conducted a pot experiment on saplings of five tree species. Before the onset of drought, we measured a set of hydraulic traits, including minimum leaf conductance, leaf embolism resistance and turgor loss point. During drought stress, we monitored traits linked to leaf hydraulic functioning (leaf water potential (ψmd) and stomatal conductance (gs)) and traits linked to leaf photochemical functioning (maximum quantum yield of photosystem II (Fv/Fm) and maximum electron transport rate (ETRmax)) at different wilting stages. After re-watering, the same traits were measured after 3, 7 and 14days. Hydraulic trait values decreased faster than photochemical trait values. After re-watering, the values of the four traits recovered at different rates. Fv/Fm recovered very fast close to their initial values only 3 days after re-watering. This was followed by ETRmax, Ψmd and gs. Finally, we show that species with large stomatal and leaf safety margin and low πtlp are not strongly impacted by drought, whereas they have a low recovery on photochemical efficiency. These results demonstrate that πtlp, stomatal and leaf safety margin are a good indicators of plant responses to drought stress and also to recovery for photochemical efficiency.

Synergistic Effects of Melatonin and Gamma-Aminobutyric Acid on Protection of Photosynthesis System in Response to Multiple Abiotic Stressors

GABA (gamma-aminobutyric acid) and melatonin are endogenous compounds that enhance plant responses to abiotic stresses. The response of Vicia faba to different stressors (salinity (NaCl), poly ethylene glycol (PEG), and sulfur dioxide (SO2)) was studied after priming with sole application of GABA and melatonin or their co-application (GABA + melatonin). Both melatonin and GABA and their co-application increased leaf area, number of flowers, shoot dry and fresh weight, and total biomass. Plants treated with GABA, melatonin, and GABA + melatonin developed larger stomata with wider aperture compared to the stomata of control plants. The functionality of the photosynthetic system was improved in primed plants. To investigate the photosynthetic functionality in details, the leaf samples of primed plants were exposed to different stressors, including SO2, PEG, and NaCl. The maximum quantum yield of photosystem II (PS II) was higher in the leaf samples of primed plants, while the non-photochemical quenching (NPQ) of primed plants was decreased when leaf samples were exposed to the stressors. Correlation analysis showed the association of initial PIabs with post-stress FV/FM and NPQ. Stressors attenuated the association of initial PIabs with both FV/FM and NPQ, while priming plants with GABA, melatonin, or GABA + melatonin minimized the effect of stressors by attenuating these correlations. In conclusion, priming plants with both GABA and melatonin improved growth and photosynthetic performance of Vicia faba and mitigated the effects of abiotic stressors on the photosynthetic performance.

Upward LED Lighting from the Base Suppresses Senescence of Lower Leaves and Promotes Flowering in Indoor Rose Management

In indoor environments such as hotels, the light intensity is generally insufficient for managing plants, and flower buds often fail to open. Lamps placed above (downward lighting) take up space. We assessed the applicability of lighting from underneath (upward lighting) for the indoor management of roses. We grew plants indoors in dim light for 2 weeks under three conditions: 1) without supplemental lighting, 2) with downward light-emitting diode (LED) lighting, and 3) with LED lighting. We quantified photosynthetic components (chlorophyll and rubisco) and the maximum quantum yield of photosystem II (Fv/Fm, an indicator of plant health) to determine the effects of each treatment on the quality and photosynthetic abilities of the leaves. We determined the ratios of dead and opened flower buds to elucidate the effects of supplemental lighting on flower bud maturation. Management without supplemental lighting decreased the number of flowers and resulted in lower-leaf senescence. Downward LED lighting promoted blooming but also resulted in lower-leaf senescence. However, upward LED lighting promoted blooming and maintained the photosynthetic abilities of the leaves, including the lower leaves. This study shows a strong case for using upward LED lighting in appropriate settings for indoor plant management and LED-based horticulture.

Thermal Analysis of Stomatal Response under Salinity and High Light.

A non-destructive thermal imaging method was used to study the stomatal response of salt-treated Arabidopsis thaliana plants to excessive light. The plants were exposed to different levels of salt concentrations (0, 75, 150, and 220 mM NaCl). Time-dependent thermograms showed the changes in the temperature distribution over the lamina and provided new insights into the acute light-induced temporary response of Arabidopsis under short-term salinity. The initial response of plants, which was associated with stomatal aperture, revealed an exponential growth in temperature kinetics. Using a single-exponential function, we estimated the time constants of thermal courses of plants exposed to acute high light. The saline-induced impairment in stomatal movement caused the reduced stomatal conductance and transpiration rate. Limited transpiration of NaCl-treated plants resulted in an increased rosette temperature and decreased thermal time constants as compared to the controls. The net CO2 assimilation rate decreased for plants exposed to 220 mM NaCl; in the case of 75 mM NaCl treatment, an increase was observed. A significant decline in the maximal quantum yield of photosystem II under excessive light was noticeable for the control and NaCl-treated plants. This study provides evidence that thermal imaging as a highly sensitive technique may be useful for analyzing the stomatal aperture and movement under dynamic environmental conditions.

Effect of applying a calcined kaolin-based particle film on the photosynthetic capacity and growth of young eucalyptus plants

In scenarios of climatic change when increased global temperatures can be expected, it is essential to search for technologies that favor sapling survival and growth after planting and increase yield in the field. Kaolin-based particle films (KBPF) have been applied as barriers against the deleterious effects of high levels of solar radiation. The objective of the present study was to assess the effects of applying purified calcined kaolin-based particle film to young eucalyptus plants. Five treatments were carried out: 0% (just water), 3%, 5%, 7%, and 10% calcined kaolin applied to the adaxial part of the leaves. A complete randomized block design was used with five treatments and ten replicates. The plants were assessed for height, diameter at ground level, gas exchanges (net photosynthetic CO2 assimilation, stomatal conductance and transpiration), instantaneous (WUE) and intrinsic water use efficiency (IWUE), chlorophyll a fluorescence (maximum quantum yield of photosystem II (PSII) (Fv/Fm), concentration of active reaction centers in relation to the quantity of photons absorbed (RC/ABS) and the maximum ratio of quantum yields of photochemical and concurrent non-photochemical processes in PSII (Fv/F0), SPAD reading and leaf ontogeny. The 3% KBPF concentration showed the best responses in biometric assessments 80 days after planting (DAP) and were corroborated by the responses of the leaf, stem and shoot dry matter production as a whole. The leaf ontogeny assessments showed positive responses following KBPF application when considering leaf development, with 7% KBPF concentration resulting in the highest mean values. The mean specific leaf mass had negative response to high KBPF concentrations. At 60 DAP, the gas exchange variables during both assessment periods declined with an increase in KBPF concentration. Significant differences as a result of KBPF applications were found only at the start of the assessments (34 DAP) for both WUE and for IWUE. Leaf ‘greenness’ (SPAD reading) at 47 days showed a quadratic relationship in both periods. The variables of chlorophyll fluorescence showed a linear response at 34 DAP and a quadratic response 60 DAP. KBPF application increased height and diameter growth in plants treated with 3% KBPF but this response was not associated with photochemical efficiency and photosynthetic carbon assimilation values on a single-leaf basis. The best performance among the variables was provided by 3% KBPF application.

Salinity Stress Affects Photosynthesis, Malondialdehyde Formation, and Proline Content in Portulaca oleracea L.

In this investigation, the effect of salt stress on Portulaca oleracea L. was monitored at salinity levels of 100 and 300 mM NaCl. At a concentration of 100 mM NaCl there was a decrease in stomatal conductance (gs) simultaneously with an increase in CO2 assimilation (A) at the beginning of salt exposure (day 3). However, the leaf water potential (ψw), the substomatal concentration of CO2 (Ci), the maximum quantum yield of photosystem II (Fv/Fm), and the proline and malondialdehyde (MDA) content remained unchanged. Exposure to 300 mM NaCl caused a decrease in gs from day 3 and a decrease in water potential, CO2 assimilation, and Fv/Fm from day 9. There was a large increase in proline content and a significantly higher MDA concentration on days 6 and 9 of salt stress compared to the control group. After 22 days of exposure to 300 mM NaCl, there was a transition from the C4 cycle to crassulacean acid metabolism (CAM), manifested by a rapid increase in substomatal CO2 concentration and negative CO2 assimilation values. These results document the tolerance of P. oleracea to a lower level of salt stress and the possibility of its use in saline localities.

Foliar applications of urea and melatonin to alleviate waterlogging stress on photosynthesis and antioxidant metabolism in sorghum seedlings

Waterlogging represents a serious abiotic stress for plants that retards crop growth and reduces production. Therefore, exploration of effective ways to alleviate the impacts of waterlogging has important theoretical and practical applications. This study investigated the responses of seedlings of two sorghum cultivars with different waterlogging tolerances to exogenous foliar treatment with melatonin (MT) and nitrogen (N) under waterlogged conditions. The chlorophyll fluorescence, photosynthetic capabilities, and antioxidant enzyme activities of these seedlings were measured after treatments with waterlogging and either exogenous application of MT, N, or both. The results showed that waterlogging severely limited sorghum growth and reduced dry matter accumulation in both cultivars. However, the negative effects of waterlogging were mitigated by spaying MT and N on leaves, especially of the waterlogging sensitive cultivar. Notably, exogenous foliar spraying of MT and N enhanced the chlorophyll content and improved the maximal quantum yield of photosystem II photochemistry (Fv/Fm), resulting in higher rates of photosynthesis. In addition, exogenous spraying of MT and N markedly decreased the malondialdehyde (MDA) content by upregulating antioxidant enzyme activities. The results also show that the combined effects of MT and N were better than the application of either alone and that exogenous foliar spraying of MT and N significantly increased the soluble protein content of leaves of sorghum. Overall, these results indicate the beneficial roles of MT and N in alleviating waterlogging stress in sorghum and show that their application may represent a strategy to mitigate waterlogging stress during agricultural production.

Biochemical and physiological changes in maize plants supplied with silicon and infected by Exserohilum turcicum

AbstractNorthern leaf blight (NLB), caused by Exserohilum turcicum, is one of the most important diseases affecting maize production worldwide. The effect of silicon (Si) on maize resistance against NLB was investigated through an in‐depth analysis of physiological and biochemical changes (measurements of gas exchange and chlorophyll (Chl) a fluorescence parameters, activities of host defence enzymes and those involved in the antioxidative metabolism, and the determination of other metabolites) on plants non‐supplied (−Si) or supplied (+Si) with Si and inoculated with E. turcicum. Higher foliar Si concentration contributed to reducing the foliar NLB symptoms in +Si plants. Both antioxidant (ascorbate peroxidase, catalase, glutathione reductase and peroxidase) and host defence (chitinase, β‐1,3‐glucanase, polyphenoloxidase and phenylalanine ammonia‐lyase) enzymes had their activities greater for +Si plants compared to −Si plants. Lower levels of hydrogen peroxide and malondialdehyde were obtained for +Si plants in contrast to −Si plants. Net CO2 assimilation rate, stomatal conductance to water vapour, transpiration rate and internal CO2 concentration had their values significantly increased for +Si plants compared to −Si plants from 5 to 20 days after inoculation. The maximum quantum yield of photosystem II, effective quantum yield of the photosystem, quantum yield of regulated energy dissipation and quantum yield of non‐regulated energy dissipation were less impacted on the leaves of +Si plants compared to −Si plants. The Chl a concentration was kept higher for +Si plants than for −Si plants. Taken together, this study brings biochemical and physiological pieces of evidence that a more robust antioxidative metabolism, great activities of host defence‐related enzymes and the preservation of the photosynthetic machinery were all potentiated by Si contributing, therefore, for less NLB symptoms.

Role of flavonoids and proline in the protection of photosynthetic apparatus in Paulownia under salt stress

The effects of different NaCl concentrations (0–150 mM) for different periods of time (10 days and 25 days) on Paulownia tomentosa x fortunei and Paulownia elongata x elongata were analyzed. The decrease of the pigment composition, an inhibition of the photochemistry of both photosystems as well as an increase of the antioxidant activity (FRAP assay), the antiradical activity (DPPH assay), the total flavonoids and the proline levels were affected only at short salt treatment (10 days) with high NaCl concentration (150 mM). These changes correspond with some decrease of the maximum quantum yields of photosystem II (Fv/Fm), the effective quantum yield of photochemical energy conversion (ФPSII) and the photochemical quenching (qP) as a result of a restriction of electron flow from QA to QB as well as an influence on the oxidation reduction properties of P700. The role of the carotenoids and levels of flavonoids and proline during the first days of salt stress for the prevence of the functions of the photosynthetic apparatus and adaptation of plants to different high salt contents are dicussed.

Effects of nitrogen fertilization on the growth and on photochemical efficiency in plants of Handroanthus heptaphyllus

Handroanthus heptaphyllus is a tree species with wood of high economic value, used in the manufacture of furniture and civil construction. When grown in soils with low availability of natural nitrogen (N), it demonstrates low growth potential. Thus, the study aimed to define the growth and physiological responses in H. heptaphyllus plants subjeted to N fertilization after planting. Five rates of N (0, 45, 90, 180 and 270 kg N ha−1) were tested at three vegetative phases of the plants (6, 12 and 18 months after planting). At each age, the height, stem diameter, concentration of N and photosynthetic pigments in leaves, chlorophyll a fluorescence and total N content in soil and tissue were determined. The addition of N increased total N content in the soil and the leaves, the concentration of photosynthetic pigments, the use of light energy and the maximum quantum yield of photosystem II, resulting in greater plant growth. In the period between the 6th and the 18th month after planting, the plants showed an increase of 135 cm in height. The concentrations of N and photosynthetic pigments in the leaves decreased over time, because of the effect of dilution and remobilization of N. The use of 260.0 kg N ha−1 kg divided in 35% of the dose added in small holes in the soil at planting; 32.5% three months after the first application; and the remainder in the sixth month after planting increased growth in height and stem diameter at 18 months after planting.

Optimizing antioxidant activity in Agarophyton vermiculophyllum for functional packaging

The value of macroalgae in a healthy human diet is becoming increasingly recognized and supported throughout Europe. Macroalgae provide a rich source of vitamins, minerals, proteins, fatty acids, and antioxidants that also support the functionality of macroalgae in other industries, including cosmeceuticals, pharmaceuticals, and more recently, packaging. Sustainable aquaculture of macroalgae will be necessary to supply the increasing demand for macroalgae as a functional material, considering that natural harvests are limited and cannot keep up with demand. Different methods can be used to cultivate macroalgae, including flow-through systems or recirculating aquaculture systems (RAS) with natural or artificial seawater. The latter provides strict control over the growth conditions and water quality in order to provide a high quality and traceable product. Additionally, environmental conditions such as salinity, temperature, and light can be modified to optimize the concentration of functional ingredients in macroalgae. While most research efforts have focused on seasonal and geographic trends in concentrations of functional ingredients in wild macroalgae, there is less information available on optimizing these functional ingredients in aquaculture. Therefore, we performed controlled experiments to optimize the activity of antioxidants in Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N.Norris et Fredericq comb. nov. (formerly Gracilaria vermiculophylla) grown in RAS with artificial seawater and commercial fertilizer. We show that the free radical scavenging activity could be increased by 13% via high salinity, and up to 34% by increasing the light intensity, but not daily light dose, for a period of 7 days. We also monitored growth rates and the maximum quantum yield of photosystem II (Fv/Fm) and show that the conditions for optimizing antioxidant activity are not optimal for growth or photosynthesis. We therefore suggest an optimization period of 4–7 days exposure to high light on a 6:18 hour light:dark cycle prior to harvesting in order to increase antioxidant activity.

High nitrogen inhibits photosynthetic performance in a shade-tolerant and N-sensitive species Panax notoginseng.

Nitrogen (N) is a primary factor limiting leaf photosynthesis. However, the mechanism of high-N-driven inhibition on photosynthetic efficiency and photoprotection is still unclear in the shade-tolerant and N-sensitive species such as Panax notoginseng. Leaf chlorophyll (Chl) content, Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) activity and content, N allocation in the photosynthetic apparatus, photosynthetic performance and Chl fluorescence were comparatively analyzed in a shade-tolerant and N-sensitive species P. notoginseng grown under the levels of moderate nitrogen (MN) and high nitrogen (HN). The results showed that Rubisco content, Chl content and specific leaf nitrogen (SLN) were greater in the HN individuals. Rubisco activity, net photosynthetic rate (Anet), photosynthetic N use efficiency (PNUE), maximum carboxylation rate (Vcmax) and maximum electron transport rate (Jmax) were lower when plants were exposed to HN as compared with ones to MN. A large proportion of leaf N was allocated to the carboxylation component under the levels of MN. More N was only served as a form of N storage and not contributed to photosynthesis in HN individuals. Compared with the MN plants, the maximum quantum yield of photosystem II (Fv/Fm), non-photochemical quenching of PSII (NPQ), effective quantum yield and electron transport rate were obviously reduced in the HN plants. Cycle electron flow (CEF) was considerably enhanced in the MN individuals. There was not a significant difference in maximum photo-oxidation P700+ (Pm) between the HN and MN individuals. Most importantly, the HN individuals showed higher K phase in the fast chlorophyll fluorescence induction kinetic curve (OJIP kinetic curve) than the MN ones. The results obtained suggest that photosynthetic capacity might be primarily inhibited by the inactivated Rubisco in the HN individuals, and HN-induced depression of photoprotection might be caused by the photodamage to the donor side of PSII oxygen-evolving complex.

Roles of Si and SiNPs in Improving Thermotolerance of Wheat Photosynthetic Machinery via Upregulation of PsbH, PsbB and PsbD Genes Encoding PSII Core Proteins

Photosystem II is extremely susceptible to environmental alterations, particularly high temperatures. The maintenance of an efficient photosynthetic system under stress conditions is one of the main issues for plants to attain their required energy. Nowadays, searching for stress alleviators is the main goal for maintaining photosynthetic system productivity and, thereby, crop yield under global climate change. Potassium silicate (K2SiO3, 1.5 mM) and silicon dioxide nanoparticles (SiO2NPs, 1.66 mM) were used to mitigate the negative impacts of heat stress (45 °C, 5 h) on wheat (Triticum aestivum L.) cv. (Shandawelly) seedlings. The results showed that K2SiO3 and SiO2NPs diminished leaf rolling symptoms and electrolyte leakage (EL) of heat-stressed wheat leaves. Furthermore, the maximum quantum yield of photosystem II (Fv/Fm) and the performance index (PIabs), as well as the photosynthetic pigments and organic solutes including soluble sugars, sucrose, and proline accumulation, were increased in K2SiO3 and SiO2NPs stressed leaves. At the molecular level, RT-PCR analysis showed that K2SiO3 and SiO2NPs treatments stimulated the overexpression of PsbH, PsbB, and PsbD genes. Notably, this investigation indicated that K2SiO3 was more effective in improving wheat thermotolerance compared to SiO2NPs. The application of K2SiO3 and SiO2NPs may be one of the proposed approaches to improve crop growth and productivity to tolerate climatic change.

Enhancement of Photosynthetic Iron-Use Efficiency Is an Important Trait of Hordeum vulgare for Adaptation of Photosystems to Iron Deficiency.

Leaf iron (Fe) contents in Fe-deficiency-tolerant plants are not necessarily higher than that in Fe-deficiency-susceptible ones, suggesting an unknown mechanism involved in saving and allowing the efficient use of minimal Fe. To quantitatively evaluate the difference in Fe economy for photosynthesis, we compared the ratio of CO2 assimilation rate to Fe content in newly developed leaves as a novel index of photosynthetic iron-use efficiency (PIUE) among 23 different barley (Hordeum vulgare L.) varieties. Notably, varieties originating from areas with alkaline soil increased PIUE in response to Fe-deficiency, suggesting that PIUE enhancement is a crucial and genetically inherent trait for acclimation to Fe-deficient environments. Multivariate analyses revealed that the ability to increase PIUE was correlated with photochemical quenching (qP), which is a coefficient of light energy used in photosynthesis. Nevertheless, the maximal quantum yield of photosystem II (PSII) photochemistry, non-photochemical quenching, and quantum yield of carbon assimilation showed a relatively low correlation with PIUE. This result suggests that the ability of Fe-deficiency-tolerant varieties of barley to increase PIUE is related to optimizing the electron flow downstream of PSII, including cytochrome b6f and photosystem I.

New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics

Coastal hypoxia/anoxia is a major emerging threat to global coastal ecosystems. Macroalgae blooms of tens of kilometers are often observed in open waters. These blooms not only cause a lack of oxygen, but also benthic light limitation. We explored the physiological responses of Zostera marina L. to anoxia under darkness. After exposing Z. marina to anoxia under darkness for 72 h, we measured the elongation of leaves and the decrease in maximal quantum yield of photosystem II (Fv/Fm), and investigated the transcriptomic and metabolomic responses to anoxic stress based on RNA-sequencing and liquid chromatography-mass spectrometry (LC-MS) technology. The results showed that anoxic stress significantly reduced the leaf Fv/Fm, and had a significant negative effect on the photosynthesis and growth of Z. marina. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of up-regulated differentially expressed genes (DEGs) showed that glycolysis was the most significant enrichment pathway (p < 0.001), and most of the important products in glycolysis were significantly up-regulated. This indicated that the glycolysis process of anaerobic respiration is promoted under anoxia. The metabolite results also showed that glyceraldehyde 3-phosphate in the glycolysis pathway was significantly up-regulated. Moreover, three genes encoding sucrose synthase (gene-ZOSMA_310G00150, gene-ZOSMA_81G00980, and gene-ZOSMA_8G00730) and one gene encoding alpha-amylase (gene-ZOSMA_95G00270) were significantly up-regulated, providing the sugar basis for the subsequent increase in glycolysis. Furthermore, gene-encoding oxoglutarate dehydrogenase, the rate-limiting step of the tricarboxylic acid (TCA) cycle, was significantly down-regulated, indicating that this cycle was inhibited under anoxia. Metabolomic results showed that L-tryptophan, L-phenylalanine, and DL-leucine were significantly up-regulated. Only significantly decreased glutamate and non-significantly decreased glutamine, substances consumed in alanine and γ-aminobutyric acid (GABA) shunt mechanisms, were detected in the leaves, while GABA and alanine were not detected. The results of this study show that anoxic stress induces a programmed transcriptomic and metabolomic response in seagrass, most likely reflecting a complex strategy of acclimation and adaptation in seagrass to resist anoxic stress.