Introduction: The purpose of this in vitro study was to test the influence of the margin design of implant supported monolithic zirconia crowns and test if monolithic zirconia crowns with extra fine finish line thickness cemented on zirconia implant abutments can bare the maximum masticatory forces on molars and hence be used in the posterior region. Methods: Fourteen identical monolithic zirconia crowns cemented on zirconia implant abutments mounted on titanium bases and fixed on implant replicas embedded in PMMA resin were divided into two groups: group I designed with a 0.5 mm CFL (CFL) and group II with a feather-edge finish line (FEFL) of 0.3 mm. All specimens underwent static load until fracture in order to determine the break force. Break forces in N were recorded for each group. Results: The CFL group exhibited slightly higher fracture resistance (1879.14 ± 322.28 N) compared to the feather-edge finish line group (1685.00 ± 362.18 N). However, statistical analysis revealed that the observed difference between the two groups (194.14 ± 183.24; p = 0.310) was not significant. Conclusions: Within the limitations of this study, both monolithic zirconia crowns with 0.5 mm chamfer and 0.3 mm feather edge margins cemented on zirconia implant abutments can be used in the posterior region as their resistance to fracture is similar and surpasses the maximum masticatory force applied on molars.