Aim: The purpose of this study was to investigate the influence of residual endodontic sealers on the adhesion of post luting cements. For hence, dentinal tubule penetration and bonding ability of three different self-adhesive resin cements was compared. Panavia SA; Kuraray, Tokyo, Japan. Relyx U200; 3M ESPE, Seefeld, Germany. Maxcem Elite; Kerr, CA, USA Material and Method: 180 mandibular second premolar teeth roots were used. 45 roots which were instrumented and obturated with gutta-percha and AH Plus sealer which were labeled with 0.1% fluorescein dye. Forty-five roots were only instrumented with ProTaper, but left not-filled. The teeth were divided into two main groups: root canal filling FR and no root canal filling NFR . Then, these roots were cemented with three different self-adhesive resin cements.The specimens were divided into 6 groups.Group 1: Filled root FR / Panavia SA Group 2: Not-filled root NFR / Panavia SA; Group 3:FR/RelyX U200Group 4: NFT/RelyX U200Group 5: FR/Maxcem EliteGroup 6: NFR/Maxcem Elite. Before the insertion of the posts, cement was labeled with Rhodamin B. After cementation process of the fiber posts, the specimens were cross sectioned and the slices were submitted to the push-out test and dentin tubule penetration evaluation of the cements using confocal laser scanning microscopy. Results: FR/Maxcem Elite cement group presented the lowest bond strength value to dentin in the cervical third P 0.05 . Conclusion: The NFR groups showed better dentin tubule penetration values than FR groups