Abstract
The aim of this study was to evaluate the potential cytotoxicity of self-adhesive resin cements with or without light irradiation on human periodontal ligament fibroblasts (HPDLFs) in vitro. Three self-adhesive resin cements (RelyX U200, Maxcem Elite and Multilink Speed) were cured with light or not. Cured cements were stored at 37°C for 24 h in water or Dulbecco's modified eagle medium (DMEM) medium. Their chromatographic analysis of water-based extract solution was made and then the DMEM-based extract solution was diluted in complete DMEM {1:5, 1:10, 1:20, 1:40, 1:80 (v/v)} for evaluating cell relative growth rate and cell apoptosis/necrosis rate of HPDLFs. The data was analyzed by one-way ANOVA and independent T test. Regardless of light irradiation, cell relative growth rate increased, and the apoptosis/necrosis rate of each resin cement decreased with the increase of gradient dilution. Regardless of gradient dilution, the cell relative growth rate and apoptosis/necrosis rate of RelyX U200 and Maxcem Elite with light irradiation were higher than those without light irradiation. Besides, without light irradiation, Multilink Speed showed higher cell relative growth rate and lower apoptosis/necrosis rate than other cements. Light irradiation and composition difference of self-adhesive resin cements could affect their cytotoxicity on HPDLFs.
Highlights
Self-adhesive resin cements, defined as cements based on filled polymers designed to adhere to tooth structure without the requirement of a separate adhesive or etchant, were introduced to dentistry within the past decade but have gained rapidly in popularity
The concentration of the acidic monomers in the selfadhesive resin cements should be sufficiently high to achieve an acceptable bonding to the dentin and enamel; as the acid functionality is consumed through reaction with calcium on the tooth and a variety of metal oxides from the ion-leachable filler, these materials become more hydrophobic [3]
Three resin cements without light irradiation showed higher absorption peak value than them with light irradiation
Summary
Self-adhesive resin cements, defined as cements based on filled polymers designed to adhere to tooth structure without the requirement of a separate adhesive or etchant, were introduced to dentistry within the past decade but have gained rapidly in popularity. The incorporation of acid functional monomer is a critical component in self-adhesive resin cements, which could not demineralize/dissolve the smear layer completely to obtain mechanical retention [1, 2], while could form effective chemical bonding to the tooth by the acid-base neutralization reaction like glass ionomer cement [3]. Other properties of self-adhesive resin cements such as solubility, absorption, and polymerization capability, could be related to their clinical behaviors. The concentration of the acidic monomers in the selfadhesive resin cements should be sufficiently high to achieve an acceptable bonding to the dentin and enamel; as the acid functionality is consumed through reaction with calcium on the tooth and a variety of metal oxides from the ion-leachable filler, these materials become more hydrophobic [3]. The excessive hydrophilic character of self-adhesive resin cement can cause swelling that in turn can compromise surface dimensional stability [9,10,11,12]
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