Delivery Of Material Research Articles

Sustainable valorization of waste plastic into nanostructured materials for environmental, energy, catalytic and biomedical applications: A review

The extensive production and utilization of plastic products are inevitable in the current scenario. However, the non-degradable nature of waste plastic generated after use poses a grave concern. Comprehensive efforts are being made to find viable technological solutions to manage the escalating challenge of waste plastic. This review focuses on the progress made in transformation of waste plastic into value-added nanomaterials. An overview is provided of the waste plastic issue on a global level and its ecological impacts. Currently established methodologies for waste plastic management are examined, along with their limitations. Subsequently, state-of-the-art techniques for converting waste plastic into nanostructured materials are presented, with a critical evaluation of their distinct merits and demerits. Several demonstrated technologies and case studies are discussed regarding the utilization of these nanomaterials in diverse applications, including environmental remediation, energy production and storage, catalytic processes, sensors, drug delivery, bioimaging, regenerative medicine and advanced packaging materials. Moreover, challenges and prospects in the commercial level production of waste plastic-derived nanomaterials and their adoption for industrial and practical usage are highlighted. Overall, this work underscores the potential of transforming waste plastic into nanostructured materials for multifaceted applications. The valorization approach presented here offers an integration of waste plastic management and sustainable nanotechnology. The development of such technologies should pave the way toward a circular economy and the attainment of sustainable development goals.

The Faustini Permanently Shadowed Region on the Moon

Faustini crater (41 km diameter) hosts a large (664 km2) permanently shadowed region (PSR) with a high potential to harbor water-ice deposits. One of the 13 candidate Artemis III landing areas contains a portion of the crater rim and proximal ejecta. The ShadowCam instrument aboard the Korea Pathfinder Lunar Orbiter provides detailed images of the PSR within Faustini. We characterize the terrain and thermal environment within the Faustini PSR from ShadowCam images, Lunar Reconnaissance Orbiter thermal measurements and laser ranging, and thermal modeling. Our mapping revealed three distinct areas of the floor of Faustini based on elevations, slopes, and surface roughness. These units broadly correlate with temperatures; thus, they may be influenced by variations in volatile sublimation. Crater retention and topographic diffusion rates appear to be asymmetric across the floor, likely due to differences in maximum and average temperatures. Several irregular depressions and a pronounced lobate-rim crater are consistent with subsurface ice. However, differences in the thicknesses of deposited materials on the floor may also explain the asymmetry. Additionally, zones of elevated surface roughness across Faustini appear to result from overprinted crater ray segments, possibly from Tycho and Jackson craters. Mass wasting deposits and pitting on opposite sides of the crater wall may have resulted from the low-angle delivery of material ejected by the Shackleton crater impact event, suggesting that the Artemis III candidate landing region named “Faustini Rim A” will contain material from Shackleton.

Present State in the Development of Aerogel and Xerogel and their Applications for Wastewater Treatment: A Review

Abstract: This comprehensive analysis investigates the current state of development and emerging applications of aerogels and xerogels in wastewater treatment. Aerogels and xerogels, which are characterized by their distinctive porosity architectures and extraordinary material qualities (low density and high surface area), have received much interest in recent years for their potential to transform the field of wastewater treatment. In this study, we present a complete overview of the synthesis processes and structural properties of these materials, highlighting current advancements and innovations. As adsorbents, catalysts, thermal insulation materials, or drug delivery matrices, they have been employed in a number of different disciplines. Aerogels and xerogels have demonstrated their adsorption capability by effectively collecting a wide spectrum of pollutants contained in wastewater. These include the removal of potentially hazardous and deleterious components such as metal ions and organic dyes, which are prevalent in wastewater streams, as well as other organic compounds. Our analysis not only covers the synthesis and applications of aerogels and xerogels, but it also highlights eco-friendly synthesis alternatives, in line with the growing demand for sustainable material preparation methods. Against the backdrop of rising global water concerns, this analysis highlights the promising potential of these materials to play a crucial role in providing sustainable wastewater treatment solutions, thereby establishing a critical future goal.

Delivery of DART Impact Ejecta to Mars and Earth: Opportunity for Meteor Observations

NASA’s Double Asteroid Redirection Test (DART) and ESA’s Hera missions offer a unique opportunity to investigate the delivery of impact ejecta to other celestial bodies. We performed ejecta dynamical simulations using 3 million particles categorized into three size populations (10 cm, 0.5 cm, and 30 μm) and constrained by early postimpact LICIACube observations. The main simulation explored ejecta velocities ranging from 1 to 1000 m s−1, while a secondary simulation focused on faster ejecta with velocities from 1 to 2 km s−1. We identified DART ejecta orbits compatible with the delivery of meteor-producing particles to Mars and Earth. Our results indicate the possibility of ejecta reaching the Mars Hill sphere in 13 yr for launch velocities around 450 m s−1, which is within the observed range. Some ejecta particles launched at 770 m s−1 could reach Mars's vicinity in 7 yr. Faster ejecta resulted in a higher flux delivery toward Mars and particles impacting the Earth Hill sphere above 1.5 km s−1. The delivery process is slightly sensitive to the initial observed cone range and driven by synodic periods. The launch locations for material delivery to Mars were predominantly north of the DART impact site, while they displayed a southwestern tendency for the Earth–Moon system. Larger particles exhibit a marginally greater likelihood of reaching Mars, while smaller particles favor delivery to Earth–Moon, although this effect is insignificant. To support observational campaigns for DART-created meteors, we provide comprehensive information on the encounter characteristics (orbital elements and radiants) and quantify the orbital decoherence degree of the released meteoroids.

Novel drug delivery materials: Chitosan polymers conjugated with Spondias pinnata phytocompounds for enhanced anti‐microbial and anti‐cancer properties

AbstractThe current study aimed to investigate the drug delivery potential of chitosan‐conjugated Spondias pinnata phytocompounds for anticancer and antibacterial applications. The phytochemical composition of the aqueous extract of S. pinnata plant leaves revealed seven major compounds, including stearic acid, 2H‐Indol‐2‐one, beta amyrin, oleic acid, octadecanoic acid, 7‐hexadecenoic acid, and phytol. Additionally, five minor compounds were identified through GC–MS analysis. SEM analysis of chitosan‐conjugated S. pinnata phytocompounds revealed amorphous particles. This demonstrates the attainment of optimized larger crystallites, which differ in size and shape extensively. The antioxidant potential of both the chitosan‐conjugated S. pinnata phytocompounds and S. pinnata leaf extracts was evaluated via DPPH and ABTS assays, and the results revealed that the chitosan‐conjugated S. pinnata phytocompounds exhibited significant scavenging activity, with IC50 values of 18.20 and 33.15 μg/mL, respectively. Chitosan‐conjugated S. pinnata phytocompounds also demonstrated antibacterial activity against four clinically significant infections, with zones of inhibition ranging from 16 ± 0.07, 19 ± 0.10, 17 ± 0.09, and 19 ± 0.11 mm against Escherichia coli (MTCC 452), Salmonella typhi (MTCC 733), Klebsiella pneumonia (MTCC 39), and Pseudomonas aeruginosa (MTCC 1688), respectively. Furthermore, the cytotoxicity of the chitosan‐conjugated S. pinnata phytocompounds was assessed against A549 lung cancer cells, and the results revealed a significant reduction in cell viability (33.85) at higher concentrations of 150 μg/mL. The IC50 values of S. pinnata leaf extract (149.2 mg/mL) and chitosan‐conjugated S. pinnata (126.4 mg/mL) toward A549 lung cancer cells were recorded. Overall, the results of the present study highlight the therapeutic applications of chitosan‐conjugated S. pinnata phytocompounds, particularly in the context of their anticancer and antibacterial activities.

Metode Flipped Classroom dalam Pembelajaran Maharoh Istima': Studi Kasus dan Hasil

This article deals with the application of flipped classroom methods in learning istima' maharoh (listening skills) through case studies. Maharoh istima' is an important aspect of language skills that enables students to understand intonation, pronunciation, and meaning in oral texts. However, traditional teaching methods are often inadequate due to challenges such as lack of authentic material and time constraints in the classroom. Flipped classroom offers solutions by moving the delivery of basic material outside the classroom, through video and online tasks, so that class time can be focused on interactive activities and in-depth practice. This study revealed positive results from the application of this method, with an average increase in listening test scores of 20% after students followed the flipped classroom method. Students showed more active participation and greater confidence in classroom discussions, as well as improved understanding of the material. The findings suggest that the flipped classroom method can significantly improve the effectiveness of istima maharoh learning, by providing additional advantages in the development of self-learning skills. Further research is needed to evaluate other factors that affect the effectiveness of this method and how its application can be adapted in different educational contexts.

Targeted colorectal cancer treatment: In vitro anti-cancer effects of carnosine nanoparticles supported by agar and magnetic iron oxide

The usage of peptides in the colorectal cancer (CRC) treatment promises to be a new anti-cancer therapy with improved treatment efficacy. Carnosine, a natural dipeptide molecule, has been demonstrated to be a potential anti-cancer drug. Nonetheless, it shows an exhibition of high-water solubility and is quickly degraded by carnosinase. Meanwhile, agar and magnetic iron oxide are the most used materials for drug delivery due to some of their advantages such as the low cost and the larger biocompatibility feature. The purpose of this study was to investigate the anti-cancer ability of agar-encapsulated carnosine nanoparticles (AgCa-NPs) and agar-encapsulated carnosine nanoparticles-coated magnetic iron oxide nanoparticles (AgCaN-MNPs) in human CRC cells, HCT-116. We evaluated the effects of AgCa-NPs and AgCaN-MNPs with a variety of concentrations (0, 5, 10, 15, 30, 40, or 50 mM) on HCT-116 cells after 72 h and 96 h by using MTT assay and observation cell morphology. We then analyzed the cell cycle progression and assessed the expression changes of genes related to apoptosis, autophagy, necroptosis, and angiogenesis after treatment for 96 h. The results showed that AgCa-NPs and AgCaN-MNPs in vitro study decreased HCT-116 cells viability. This effect was attributed to arrest of cell cycle, induction of programmed cell death, and suppression of angiogenesis by AgCa-NPs and AgCaN-MNPs. These findings revealed the antitumor efficacy of AgCa-NPs or AgCaN-MNPs for CRC treatment.

A fuzzy multi-objective programming model for the delivery and distribution of humanitarian relief materials

Disaster relief presents many unique logistics challenges, including damaged transportation infrastructure, multiple conflicting goals, and secondary disasters caused by spontaneous disaster relief. The delivery and distribution of humanitarian relief materials faced by nonprofit organizations (NPOs) poses a critical challenge due to the highly unpredictable nature of disasters, conflicting missions, and the need to navigate government regulations in disaster management. In this paper, we propose a novel multi-objective disaster relief modeling system for NPOs, considering (1) prioritization (providing medical services to seriously injured victims), (2) effectiveness (degree of satisfaction), (3) efficiency (operational costs), and (4) equity (cost of maintaining order). To incorporate the practical constraints of disaster relief operations, we include factors such as road damage, transportation capacity limitations, and route flow saturation as part of the model formulation. We then utilize fuzzy multi-objective programming to optimize the proposed disaster relief model, considering two relief distribution schemes: a common NPOs' spontaneous relief scheme and a newly designed traffic control scheme with government regulation. Based on the real-world scenario of the 2008 Wenchuan earthquake, we demonstrate that the designed traffic control scheme exhibited remarkable improvement among the NPOs' four objectives compared to the spontaneous relief scheme. Moreover, in the process of solving fuzzy multi-objective programming problems, artificial intelligence technology is employed to handle the fuzziness in multi-objective and automatically adjust parameters, making the solution results better and in line with practical situations. And we also validate the proposed model can be solved in polynomial time, meeting the engineering practice requirements, and can be easily extend to most disaster relief distribution scenarios.

Edukasi Bahaya Formalin, Cara Identifikasi dan Upaya Penurunan Risiko pada Produk Pangan di MAN 3 Pontianak

The use of formalin as a food additive is prohibited according to Indonesian Minister of Health Regulation No. 033 of 2012 concerning Food Additives (BTP). Due to its easy availability and its ability to extend shelf life, formalin is often misused as a food preservative. Given its carcinogenic properties, the use of formalin in food poses health risks. The purpose of this community service is to provide education on the dangers of formalin, identification techniques for formalin, and de-formalization efforts in food products. The community service was conducted on July 25, 2024, with 30 students from MAN 3 Pontianak participating. The service was carried out using an outreach method at MAN 3 Pontianak, including pre-tests, material delivery, demonstrations, and post-tests. The results from the pre-test and post-test show an average knowledge increase of 19% regarding formalin risks and reduction efforts. Following the education through material presentation and demonstrations, the number of students with good knowledge increased to 26 (86.7%), while those with insufficient knowledge decreased to 4 (13.3%). The attitude assessment revealed that all students at MAN 3 Pontianak had a positive attitude towards the dangers of formalin and efforts to reduce formalin risks in food (100%).

Pengabdian Masyarakat dengan “Pendampingan Posyandu Remaja sebagai Upaya Preventif Kenakalan Remaja di Posyandu Remaja Kelurahan Banyurip Kota Pekalongan”

Adolescence is a transition period which is a period of transition in a child's development between childhood and adulthood which generally begins at the age of 12 or 13 years and ends at the age of 21 years. Adolescence is a vulnerable period because of the inability of teenagers to solve their own problems. Many teenagers ultimately find that many of the solutions do not match their expectations. advances in science and technology always result in social change, with increasingly sophisticated communications technology, transportation and information systems, changes in society are accelerating more rapidly. In facing such situations, teenagers often have a more sensitive soul, which in the end, quite a few teenagers fall into things that are contrary to moral values, religious norms, social norms and norms of life in society. The aim of this service is to provide assistance to youth posyandu as an effort to prevent juvenile delinquency. The method used in this community service activity begins with an opening and introduction of the resource persons, then continues with the delivery of health education material, a question and answer session to the participants and finally closing. With this activity, the posyandu felt helped because it was provided with assistance and information in preventive efforts to prevent juvenile delinquency.

A Comprehensive Review of the Factors and Strategies to Mitigate Construction Projects Delays in Saudi Arabia

Background The construction industry in Saudi Arabia faces numerous challenges that hinder its progress due to frequent and prolonged project delays. Objective The specific objectives of this study are (1) (1) To analyze the factors contributing to construction project delays in Saudi Arabia, (2) To identify the risk of factors contributing to construction project delays, and (3) To propose strategies to mitigate construction project delays in Saudi Arabia. Methods The study reviewed 16 articles to identify and suggest solutions for construction project delays in Saudi Arabia. Results There are 140 factors of delay found and seven pose unacceptable risks including delays due to material delivery, delays in approval of submittals, design drawings, shop drawings, and sample materials (making decisions), delays related to subcontractors work, inflation and escalation of material prices (changes in price), lack of coordination with contractors, shortage of construction materials (non-availability of material), and equipment shortages. The risk assessment can be conducted using the Bowtie diagram, scenario analysis, MRM, and MCS separately or collectively to get a new and improved model. Conclusion This review will contribute to the development of strategies to enhance the performance of ongoing and future construction projects in the region. Additionally, decision-makers can utilize the outcomes to strategically allocate resources and improve construction project success.

Metal-ion-binding properties of glycyrrhiza polysaccharide extracted from Licorice: Structural characterization and potential application in drug delivery

Licorice is not only a widely used food, but also a classic tonic Chinese medicine, which mainly contains glycyrrhiza polysaccharides (GP) and flavonoids with excellent anti-inflammatory and antioxidant pharmacological activities. In this study, a neutral homogeneous polysaccharide (GP1–2) was isolated from Glycyrrhiza uralensis Fisch. However, its gelation behavior and properties have yet to be comprehensively studied. In this study, a Ca2+ cross-linked physical hydrogel based on neutral GP1–2 (GP1–2-Ca2+) is fabricated. The ability of metal ions to cross-linked gelation with GP1–2 is explored with respect to the polysaccharide concentrations, ion species, and pH environments. The pH range of Ca2+ cross-linked with GP1–2 to form hydrogel is 8 to 10, and the gelation concentration ranges from 20.0 % to 50.0 % w/v. Subsequently, the properties of the GP1–2-Ca2+ hydrogels are investigated using rheological measurements, scanning electron microscopy, free radical scavenging, MTT assays, healing capability, and enzyme-linked immunosorbent assays. The results reveal that the structure of GP1–2 presents an irregular porous structure, however, the physical gel formed after cross-linking with Ca2+ microscopically showed a globular porous structure with uniform distribution, suggesting that this structure characteristic may be used as a carrier material for drug delivery. Meanwhile, the GP1–2-Ca2+ hydrogel also possessed extraordinary viscoelasticity, cytocompatibility, antioxidant properties, anti-inflammatory activity, and ability to promote wound healing. Furthermore, the potential of GP1–2-Ca2+ hydrogels as drug delivery materials was validated by using rhein as a model drug for encapsulation, it is demonstrated that its cumulative release behavior of GP1–2-Ca2+ is pH-dependent. All in all, this study reveals the potential application of natural polysaccharides in drug delivery, highlighting its dual roles as carrier materials and bioactive ingredients.

Addition of an audience response system in a biomedical science course improves learning environment and student performance.

The effectiveness of incorporating an audience response system (ARS) in improving the learning environment and student performance was assessed in a didactic biomedical science course at a large US dental school. Instructors and students were surveyed for their experiences of using Top Hat-based ARS in a multi-disciplinary biomedical science course. Average exam scores and grade distribution for challenging sections on immunology and developmental biology were compared before and after incorporating ARS. Pearson's chi-squared test, likelihood ratio test, Student's t-test and Fisher's exact test using beta regression model were used to assess statistical significance (p < 0.05). Student survey results with 78% (82/105) response rate, indicated that incorporating ARS improved student engagement, reinforced lecture material, and prepared them better for exams (p < 0.0001). Sixty seven percent of student respondents recommended continued integration of ARS questions in lectures (p < 0.0001). Although faculty survey data (n = 5, 100% response rate) were not statistically significant, the majority of faculty agreed that real-time feedback through ARS allowed them to appropriately pace lecture delivery and restructure forthcoming material. The average exam scores and grade distribution for challenging sections showed modest improvement after incorporation of ARS (p < 0.05). There was no significant correlation between final course grades and ARS grades for participation or correctness. Incorporating the ARS in a multi-disciplinary biomedical science course is perceived by faculty and students as an effective instructional approach that improves the learning environment, teaching strategies, and student performance.

Peran Perguruan Tinggi Keagamaan Islam (PTKIS) Dalam Pendidikan Perspektif Gender Menuju Indonesia Emas 2045

This paper reviews the role of Islamic Higher Education Institutions (HEIs) in gender perspective education as a key component towards Indonesia Emas 2024. Gender education involves an understanding of gender roles and human rights. HEIs have a responsibility to integrate religious values with gender education, shaping leaders who are aware of gender equality, and able to address complex social challenges. HEIs can play a major role in addressing this. By understanding the concept of gender education, integrating it in the curriculum, and involving lecturers in the delivery of gender materials, PTKIS can create an inclusive and civilised educational environment. Through gender inclusivity policies, prevention of gender-based violence, and active student participation in gender equality, PTKIS has the potential to shape a more inclusive, just, and competitive society, in accordance with the vision of Indonesia Emas 2024 that reflects the values of justice, equality, and sustainable progress.

Personalization of lipid-based oral dosage forms via filament-based 3D-printing

While filament-based 3D-printing (3DP) is the most utilized 3DP technology in the pharmaceutical field, it has not been demonstrated for processing of drug-loaded lipid-based formulations. This work exploits hexa-glycerol ester of palmitic acid (Pg6-C16-P) as an advanced lipid material, loaded with felodipine as a poorly soluble model drug, for fabricating novel oral solid dosage forms (OSDFs) via filament-based 3D-printing. After material melt-blending, the formulation was extruded using the liquid feeding approach to obtain a mechanically manageable, and hence 3D-printable, drug-loaded lipid filament. The fabrication of geometries with variable infill densities was demonstrated. The extent of infill density was found to significantly impact the optimal printing parameters required to achieve the desired shape. The solid-state analysis confirmed the amorphous state of felodipine after 3DP. The release rate of the drug was studied via in vitro dissolution test and showed to be tunable based on the tablet geometry. It was also possible to tailor the design of the dosage form to perform similarly to a commercial product. The formulation was evidenced as safe via in vitro toxicity studies with improved felodipine solubility. This study establishes filament-based 3DP as an alternative platform viable for fabricating advanced lipid-based OSDFs, and concurrently, promotes Pg6-C16-P as a promising and high performing 3DP lipid material for drug delivery.

Fabrication of Hybrid Coated Microneedles with Donepezil Utilizing Digital Light Processing and Semisolid Extrusion Printing for the Management of Alzheimer's Disease.

Microneedle (MN) patches are gaining increasing attention as a cost-effective technology for delivering drugs directly into the skin. In the present study, two different 3D printing processes were utilized to produce coated MNs, namely, digital light processing (DLP) and semisolid extrusion (SSE). Donepezil (DN), a cholinesterase inhibitor administered for the treatment of Alzheimer's disease, was incorporated into the coating material. Physiochemical characterization of the coated MNs confirmed the successful incorporation of donepezil as well as the stability and suitability of the materials for transdermal delivery. Optical microscopy and SEM studies validated the uniform weight distribution and precise dimensions of the MN arrays, while mechanical testing ensured the MNs' robustness, ensuring efficient skin penetration. In vitro studies were conducted to evaluate the produced transdermal patches, indicating their potential use in clinical treatment. Permeation studies revealed a significant increase in DN permeation compared to plain coating material, affirming the effectiveness of the MNs in enhancing transdermal drug delivery. Confocal laser scanning microscopy (CLSM) elucidated the distribution of the API, within skin layers, demonstrating sustained drug release and transcellular transport pathways. Finally, cell studies were also conducted on NIH3T3 fibroblasts to evaluate the biocompatibility and safety of the printed objects for transdermal applications.

Teknologi dan Personalisasi Pembelajaran Pendidikan Islam untuk Generasi Z

This study discusses the integration of technology and personalized learning in Islamic education for Generation Z. The research employs a qualitative approach with literature review as the main method. The findings indicate that technology can enhance learning effectiveness by providing interactive tools and platforms accessible anytime and anywhere. Technology allows for more engaging material delivery and helps students understand Islamic concepts through visualization and simulation. Personalized learning allows curriculum and teaching methods to be tailored to individual student needs, which can increase their motivation and learning outcomes. Generation Z, who have grown up in a digital environment, greatly benefit from this approach. However, challenges such as the digital divide, negative impacts of technology on social and spiritual interactions, and the substantial resources required for personalized learning need to be addressed. A balanced strategy between the use of technology and the preservation of Islamic values is necessary to ensure that Islamic education remains relevant and effective in the digital era. Developing technological infrastructure, training programs for teachers and students, and relevant curricula are essential to achieving these goals.

Sodium ampicillin release from biocompatible hydrogel with enhanced antibacterial characteristics

The goal of this research is to develop an intelligent carrier that can control the release of medications in response to particular triggers. A novel carrier was created by combining different amounts of chitosan and polyvinylalcohal along with specific amount of cinnamon extract to enhance the antibacterial response of the prepared hydrogels. A non-hazardous crosslinker 3-Aminopropyl(diethoxy)methylsilane (3-APDEMS) was used to study its effect on the physical and chemical properties of the produced injectable hydrogels as well as the controlled drug release behaviour. The hydrogels swelling behaviour was assessed in a range of solvent environments and the results indicated that the swelling ratio decreased as the concentration of 3-APDEMS rose. The hydrogels showed the most swelling at acidic pH values and the least swelling at basic and neutral pH values. They are appropriate for use in injectable controlled medication delivery systems due to their unique pH-sensitive characteristic at pH 7.To evaluate its release profile in this particular setting, ampicillin sodium salt was effectively added to the best hydrogel formulation (which contained 100 μL cinnamon extract and 150 μL 3-APDEMS).The results showed that the release of ampicillin sodium (AMS) was regulated in PBS (7.4), reached up to 98.236 % release over 90 min.The utilisation of these hydrogels as a cutting-edge material for physiological pH-controlled injectable medication delivery and other biological applications is supported by these results.

Cytomegalovirus inhibitors of programmed cell death restrict antigen cross-presentation in the priming of antiviral CD8 T cells.

CD8 T cells are the predominant effector cells of adaptive immunity in preventing cytomegalovirus (CMV) multiple-organ disease caused by cytopathogenic tissue infection. The mechanism by which CMV-specific, naïve CD8 T cells become primed and clonally expand is of fundamental importance for our understanding of CMV immune control. For CD8 T-cell priming, two pathways have been identified: direct antigen presentation by infected professional antigen-presenting cells (pAPCs) and antigen cross-presentation by uninfected pAPCs that take up antigenic material derived from infected tissue cells. Studies in mouse models using murine CMV (mCMV) and precluding either pathway genetically or experimentally have shown that, in principle, both pathways can congruently generate the mouse MHC/H-2 class-I-determined epitope-specificity repertoire of the CD8 T-cell response. Recent studies, however, have shown that direct antigen presentation is the canonical pathway when both are accessible. This raised the question of why antigen cross-presentation is ineffective even under conditions of high virus replication thought to provide high amounts of antigenic material for feeding cross-presenting pAPCs. As delivery of antigenic material for cross-presentation is associated with programmed cell death, and as CMVs encode inhibitors of different cell death pathways, we pursued the idea that these inhibitors restrict antigen delivery and thus CD8 T-cell priming by cross-presentation. To test this hypothesis, we compared the CD8 T-cell responses to recombinant mCMVs lacking expression of the apoptosis-inhibiting protein M36 or the necroptosis-inhibiting protein M45 with responses to wild-type mCMV and revertant viruses expressing the respective cell death inhibitors. The data reveal that increased programmed cell death improves CD8 T-cell priming in mice capable of antigen cross-presentation but not in a mutant mouse strain unable to cross-present. These findings strongly support the conclusion that CMV cell death inhibitors restrict the priming of CD8 T cells by antigen cross-presentation.

Pelatihan Pembuatan PMT Nugget dan Sempol Sehat Berbasis Pangan Lokal untuk Mencegah Diare dan Stunting di Desa Mandiro, Kabupaten Bondowoso

Diarrhea can cause disruption of nutrient absorption in the body, which can result in growth failure if not treated immediately. When toddlers experience diarrhea, they will experience a decrease in appetite so that food intake is not optimal. In an effort to prevent diarrhea and stunting in Mandiro Village, the PPK Ormawa Team of HIMA Gizi FKM UNEJ provided training in making PMT based on local food, namely moringa leaf carrot chicken nuggets and cassava leaf chicken sempol to Posyandu Cadres and mothers of toddlers in Mandiro Village. The delivery of training materials is carried out using expository and demonstration methods. Monitoring and evaluation of the activities are conducted using questionnaires in the form of pre-tests and post-tests. The results of the pre-tests and post-tests indicate an increase in respondents' knowledge related to Supplementary Feeding (PMT).