Biomedical Materials Research Research Articles

Comparative In Vivo Biocompatibility of Cellulose-Derived and Synthetic Meshes in Subcutaneous Transplantation Models.

Despite the increasing interest in cellulose-derived materials in biomedical research, there remains a significant gap in comprehensive in vivo analyses of cellulosic materials obtained from various sources and processing methods. To explore durable alternatives to synthetic medical meshes, we evaluated the in vivo biocompatibility of bacterial nanocellulose, regenerated cellulose, and cellulose nanofibrils in a subcutaneous transplantation model, alongside incumbent polypropylene and polydioxanone. Notably, this study demonstrates the in vivo biocompatibility of regenerated cellulose obtained through alkali dissolution and subsequent regeneration. All cellulose-derived implants triggered the expected foreign body response in the host tissue, characterized predominantly by macrophages and foreign body giant cells. Porous materials promoted cell ingrowth and biointegration. Our results highlight the potential of bacterial nanocellulose and regenerated cellulose as safe alternatives to commercial polypropylene meshes. However, the in vivo fragmentation observed for cellulose nanofibril meshes suggests the need for measures to optimize their processing and preparation.

Synthesis and in vitro study of biodegradable and thermoresponsive polymer based on polysuccinimide for drug delivery

Hydrogels, defined as polymer networks with a high water content and insoluble in water, have emerged as pivotal materials in biomedical research due to their resemblance to the natural extracellular matrix. The aim of this study is to develop an injectable, thermoresponsive, and biodegradable hydrogel. The hydrogel (PSI-t) was derived from poly (aspartic acid) (PSI) by grafting hydrophobic dodecylamine (DA) and hydrophilic ethanolamine (EA) groups onto its backbone. PSI-t was found to exhibit a unique sol state at room temperature, which facilitates facile injection, and undergoes rapid gelation at physiological temperatures, highlighting its potential as an injectable thermosensitive hydrogel for targeted drug delivery. In vitro tests confirmed the biocompatibility of the thermosensitive hydrogel, and sustained drug release studies using the model drug, acetaminophen (AP), underscored the efficacy of the hydrogel as a controlled release vehicle. Furthermore, the hydrogel was found to be easily eliminated through bio-hydrolysis, thus enabling prolonged drug effects while mitigating any potential toxicity. These findings suggest that PSI-t is a promising candidate for an innovative and injectable drug delivery system in biomedical applications.

Rare-earth nanocrystalline scintillators for biomedical application: A review

Rare-earth scintillating materials have been widely utilized in nuclear image technique, security screening and high energy physics research. Researchers have devoted extensive efforts to size design, surface modification, and drug assembly to transform them into prospective nanomaterials for diagnostics and cancer treatment. This review endeavors to summarize the advanced preparation technology and design concept of rare-earth nanocrystalline scintillators (RENSs), and offers fundamental guidance for the application of various materials in biomedical research. Specifically, it focuses on RENSs with excellent biocompatibility, outstanding stability, uniform size distribution, and strong drug loading capacity to meet subsequent practical applications. Additionally, this review reports on the application of RENSs in the biomedical field. Specifically, bioimaging and antitumor therapy are the most prevalently utilized and promising areas. X-ray excited optical imaging (XEOL) is combined with advanced imaging techniques (CT, MRI, etc.) to obtain comprehensive information about deep tissue. The combination of X-ray radiation therapy and photodynamic therapy technology can realize safe anti-tumor therapy under the condition of low-dose irradiation. Furthermore, this review also enumerates several recent highlight research strategies and the prospects of RENSs in the biomedical application in the future.

Utilizing AI-Enhanced Multi-Omics Integration for Predictive Modeling of Disease Susceptibility in Functional Phenotypes

With the continuous development of machine learning technology, the scientific research of biomedical materials is gradually shifting to a data-driven direction. The rise of this trend stems from the widespread use of Bio sequencing technology, which provides entirely new methods and insights for testing and evaluating the biological function of biomedical materials. The performance and performance of biomedical materials have a wide range of applications in medical applications, drug delivery, biosensors and other fields, so it is important to further optimize them. However, with the accumulation and increasing complexity of data, there is a need for more intelligent and efficient ways to process and analyze this heterogeneous scientific data. Therefore, the establishment of an open, shared infrastructure for storing heterogeneous scientific data from different research fields will be the cornerstone of cross-disciplinary joint analysis. This infrastructure will not only accelerate the collection and integration of data, but will also provide opportunities for collaboration and innovation across disciplines. This paper highlights a new trend in biomedical materials research, namely a data-driven approach, and the key role of Bio sequencing technology in this process. At the same time, we call for the establishment of an open data storage and sharing platform to promote multidisciplinary cooperation, accelerate the optimization and innovation of biomedical materials, and open up broader prospects for future biomedical applications. This effort is expected to push scientific research in the medical field to new heights, providing safer and more effective treatments and medical programs for patients.

308. Injectable Scaffold-based Vaccination For Prevention of Periprosthetic Joint Infection

Abstract Background There are currently no vaccines available to prevent periprosthetic joint infection (PJI) and existing vaccine platforms have failed against Staphylococcus aureus in clinical trials. We have developed a novel injectable scaffold-based vaccine for PJI. In this study, we evaluated vaccine immune response and bacterial burden in a murine model of PJI. Methods Vaccines consisting of mesoporous silica rods (scaffold) and demethylated CpG (adjuvant) were prepared using S. aureus-derived pathogen-associated molecular patterns (PAMPs) as described previously (Super and Doherty et al., Nat Biomed Eng 2022). Mice were either unvaccinated or vaccinated by subcutaneous scaffold injection containing different amounts of PAMP antigen: 7.5, 30, or 150 units (n=5-8 per group). Mice received a second scaffold injection on Day 14 (same dose as Day 0) as booster. Sera was collected on Day 28. For a challenge study, mice vaccinated on Days 0 and 14 with scaffold loaded with 30 U of PAMPs (“Vaccinated”) underwent implantation of a stainless-steel wire in the distal femur protruding into the joint space on Day 35 (n=8). The implant was inoculated with 1000 CFU S. aureus Xen29, a bioluminescent strain. Infected mice without vaccination (“Unvaccinated”) and without infection (“Uninfected”) also underwent implant insertion (n=5). Mice underwent bioluminescent imaging, collection of sera and tissue, and euthanasia with implant harvest on Day 49. Results Vaccination increased anti-S. aureus antibodies in a dose-dependent manner. Vaccination with Xen29 PAMPs resulted in antibodies with cross-reactivity against other S. aureus strains (RN4220, RN4220ΔSpa, and BAA1717). Vaccinated mice had increased CD4+ and CD8+ T cells in the adjacent draining lymph node. Vaccination induced higher IgG1 and IgG2b whereas infection only increased IgG2b (Fig. 1A). Vaccinated animals had bioluminescent similar to uninfected animals by Day 45 (Fig. 1B). Vaccinated animals had significantly less implant bacteria than unvaccinated animals and 25% of vaccinated animals did not have any detectable bacteria at euthanasia (Fig. 1C).Figure 1.A) Anti-S. aureus immunoglobulins pre- (Day 28) and post-infection (Day 49). B) Representative bioluminescent images at Day 42 and quantification. C) Implant bacterial burden at euthanasia (Day 49). * = p<0.05. Conclusion Scaffold-based vaccination increased generalized anti-S. aureus antibody production in an antigen dose-dependent manner and decreased bacterial burden in a murine model of PJI. Disclosures David Mooney, Ph.D., Advanced Healthcare Materials: Board Member|Agnovos: Stocks/Bonds|Attivare: Stocks/Bonds|Boston Scientific: Advisor/Consultant|Cartesian Therapeutics: Advisor/Consultant|IVIVA: Stocks/Bonds|Johnson & Johnson: Advisor/Consultant|Journal of Biomedical Materials Research: Board Member|Lightning Bio: Stocks/Bonds|Lyell: Stocks/Bonds|Medicenna: Advisor/Consultant|Norvartis: Advisor/Consultant|Norvartis: Grant/Research Support|Norvartis: IP Royalties|Revela: Stocks/Bonds|Samyang: Advisor/Consultant

Global trends in the research of biodegradable biomedical magnesium-based materials: a bibliometric analysis

Background: In recent years, there has been a notable surge in the interest surrounding biodegradable materials, particularly in the context of biomedical applications. This has led to a significant rise in the number of research studies focusing on the utilization of biodegradable magnesium-based materials in the field of biomedicine. However, a dearth of comprehensive assessment exists regarding the body of research concerning biodegradable biomedical magnesium-based materials. In this study, a bibliometric approach was used to illustrate the current state of research and global trends pertaining to biodegradable magnesium-based materials for biomedical applications.Methods: We conducted a search of the Web of Science core collection database for the past decade (2013–2022). VOSviewer software and the bibliometric online analysis platform were employed for bibliometric analysis and visualization.Results: Correspondingly, 1267 documents were retrieved. We discovered that the number of papers in the field of degradable biomedical magnesium-based materials research has increased annually. In addition, China and the Chinese Academy of Sciences have published the largest number of papers in the field of biodegradable biomedical magnesium-based materials. Papers related to biodegradable magnesium-based materials for biomedical use were mainly published in acta biomaterialia, materials science and engineering c-materials for biological applications and materials journals. Keyword co-occurrence analysis showed that “corrosion"and “mechanical-properties” appear more frequently. The top 10 common keywords include corrosion, mechanical-properties, microstructure, biocompatibility, behavior, magnesium, magnesium alloys, degradation magnesium alloy, in vitro.Conclusion: Research on biodegradable magnesium-based materials for biomedical use continues to increase steadily. China maintains a leading position in the world, and the Chinese Academy of Sciences represents a notable contribution to the research of biodegradable magnesium-based materials for biomedical use. Subsequently, “corrosion” and “mechanical-properties” were identified as the current research hotspots in the area of biodegradable biomedical magnesium-based materials.

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Journal of Biomedical Materials Research Part AVolume 111, Issue 8 p. 1091-1095 ISSUE INFORMATIONFree Access Issue Information First published: 16 June 2023 https://doi.org/10.1002/jbm.a.37404AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Volume111, Issue8August 2023Pages 1091-1095 RelatedInformation

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 111, Issue 7 p. 1337-1341 ISSUE INFORMATIONFree Access Issue Information First published: 09 May 2023 https://doi.org/10.1002/jbm.b.35094AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Volume111, Issue7July 2023Pages 1337-1341 RelatedInformation

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Journal of Biomedical Materials Research Part AVolume 111, Issue 6 p. 741-745 ISSUE INFORMATIONFree Access Issue Information First published: 13 April 2023 https://doi.org/10.1002/jbm.a.37402AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Volume111, Issue6Special Issue: 2022 Emerging Scholars in BiomaterialsJune 2023Pages 741-745 RelatedInformation

Properties of Poly (Lactic-co-Glycolic Acid) and Progress of Poly (Lactic-co-Glycolic Acid)-Based Biodegradable Materials in Biomedical Research.

In recent years, biodegradable polymers have gained the attention of many researchers for their promising applications, especially in drug delivery, due to their good biocompatibility and designable degradation time. Poly (lactic-co-glycolic acid) (PLGA) is a biodegradable functional polymer made from the polymerization of lactic acid (LA) and glycolic acid (GA) and is widely used in pharmaceuticals and medical engineering materials because of its biocompatibility, non-toxicity, and good plasticity. The aim of this review is to illustrate the progress of research on PLGA in biomedical applications, as well as its shortcomings, to provide some assistance for its future research development.

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Journal of Biomedical Materials Research Part AVolume 111, Issue 5 p. 575-579 ISSUE INFORMATIONFree Access Issue Information First published: 16 March 2023 https://doi.org/10.1002/jbm.a.37401AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume111, Issue5May 2023Pages 575-579 RelatedInformation

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 111, Issue 5 p. 981-986 ISSUE INFORMATIONFree Access Issue Information First published: 03 March 2023 https://doi.org/10.1002/jbm.b.35092AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume111, Issue5May 2023Pages 981-986 RelatedInformation

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 111, Issue 3 p. 499-504 ISSUE INFORMATIONFree Access Issue Information First published: 18 January 2023 https://doi.org/10.1002/jbm.b.35090AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume111, Issue3March 2023Pages 499-504 RelatedInformation

Knowledge domain and hotspots concerning photosensitive hydrogels for tissue engineering applications: A bibliometric and visualized analysis (1996-2022).

Objective: The aim of tissue engineering (TE) is to replace the damaged tissues or failed organs, or restore their missing functions. The important means to achieve this aim is to integrate biomaterials and life elements. Hydrogels are very attractive biomaterials in the field of TE. In particular, engineering extracellular matrices (ECMs) formed by photosensitive hydrogels have captivated much attention, because photopolymerization has many advantages over traditional polymerization approaches, such as rapidity of reaction, spatiotemporal controllability of polymerization process, and operability at physiological temperature, especially it can realize the fabrications of engineering ECMs in the presence of living cells. There have been many excellent reviews on the applications of photosensitive hydrogels in TE in recent years, however, it is inevitable that researchers may have left out many important facts due to exploring the literature from one or a few aspects. It is also a great challenge for researchers to explore the internal relationships among countries, institutions, authors, and references from a large number of literatures in related fields. Therefore, bibliometrics may be a powerful tool to solve the above problems. A bibliometric and visualized analysis of publications concerning the photosensitive hydrogels for TE applications was performed, and the knowledge domain, research hotspots and frontiers in this topic were identified according to the analysis results. Methods: We identified and retrieved the publications regarding the photosensitive hydrogels for TE applications between 1996 and 2022 from Web of Science Core Collection (WoSCC). Bibliometric and visualized analysis employing CiteSpace software and R-language package Bibliometrix were performed in this study. Results: 778 publications meeting the eligibility criteria were identified and retrieved from WoSCC. Among those, 2844 authors worldwide participated in the studies in this field, accompanied by an average annual article growth rate of 15.35%. The articles were co-authored by 800 institutions from 46 countries/regions, and the United States published the most, followed by China and South Korea. As the two countries that published the most papers, the United States and China could further strengthen cooperation in this field. Univ Colorado published the most articles (n = 150), accounting for 19.28% of the total. The articles were distributed in 112 journals, among which Biomaterials (n = 66) published the most articles, followed by Acta Biomaterialia (n = 54) and Journal of Biomedical Materials Research Part A (n = 42). The top 10 journals published 47.8% of the 778 articles. The most prolific author was Anseth K (n = 33), followed by Khademhosseini A (n = 29) and Bryant S (n = 22). A total of 1443 keywords were extracted from the 778 articles and the keyword with the highest centrality was "extracellular matrix" (centrality: 0.12). The keywords appeared recently with strong citation bursts were "gelatin", "3d printing" and "3d bioprinting", representing the current research hotspots in this field. "Gelma", "3d printing" and "thiol-ene" were the research frontiers in recent years. Conclusion: This bibliometric and visualized study offered a comprehensive understanding of publications regarding the photosensitive hydrogels for TE applications from 1996 to 2022, including the knowledge domain, research hotspots and frontiers in this filed. The outcome of this study would provide insights for scholars in the related research filed.

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 111, Issue 1 p. 1-6 ISSUE INFORMATIONFree Access Issue Information First published: 01 November 2022 https://doi.org/10.1002/jbm.b.35088AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Volume111, Issue1January 2023Pages 1-6 RelatedInformation

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 110, Issue 12 p. 2589-2594 ISSUE INFORMATIONFree Access Issue Information First published: 17 October 2022 https://doi.org/10.1002/jbm.b.34880AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume110, Issue12December 2022Pages 2589-2594 RelatedInformation

Better governance starts with better words: why responsible human tissue research demands a change of language

The rise of precision medicine has led to an unprecedented focus on human biological material in biomedical research. In addition, rapid advances in stem cell technology, regenerative medicine and synthetic biology are leading to more complex human tissue structures and new applications with tremendous potential for medicine. While promising, these developments also raise several ethical and practical challenges which have been the subject of extensive academic debate. These debates have led to increasing calls for longitudinal governance arrangements between tissue providers and biobanks that go beyond the initial moment of obtaining consent, such as closer involvement of tissue providers in what happens to their tissue, and more active participatory approaches to the governance of biobanks. However, in spite of these calls, such measures are being adopted slowly in practice, and there remains a strong tendency to focus on the consent procedure as the tool for addressing the ethical challenges of contemporary biobanking. In this paper, we argue that one of the barriers to this transition is the dominant language pervading the field of human tissue research, in which the provision of tissue is phrased as a ‘donation’ or ‘gift’, and tissue providers are referred to as ‘donors’. Because of the performative qualities of language, the effect of using ‘donation’ and ‘donor’ shapes a professional culture in which biobank participants are perceived as passive providers of tissue free from further considerations or entitlements. This hampers the kind of participatory approaches to governance that are deemed necessary to adequately address the ethical challenges currently faced in human tissue research. Rather than reinforcing this idea through language, we need to pave the way for the kind of participatory approaches to governance that are being extensively argued for by starting with the appropriate terminology.

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 110, Issue 3 p. 501-506 ISSUE INFORMATIONFree Access Issue Information First published: 20 January 2022 https://doi.org/10.1002/jbm.b.34871AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume110, Issue3March 2022Pages 501-506 RelatedInformation

Injectable Magnetic-Responsive Short-Peptide Supramolecular Hydrogels: Ex Vivo and In Vivo Evaluation.

The inclusion of magnetic nanoparticles (MNP) in a hydrogel matrix to produce magnetic hydrogels has broadened the scope of these materials in biomedical research. Embedded MNP offer the possibility to modulate the physical properties of the hydrogel remotely and on demand by applying an external magnetic field. Moreover, they enable permanent changes in the mechanical properties of the hydrogel, as well as alterations in the micro- and macroporosity of its three-dimensional (3D) structure, with the associated potential to induce anisotropy. In this work, the behavior of biocompatible and biodegradable hydrogels made with Fmoc-diphenylalanine (Fmoc-FF) (Fmoc = fluorenylmethoxycarbonyl) and Fmoc–arginine–glycine–aspartic acid (Fmoc-RGD) short peptides to which MNP were incorporated was studied in detail with physicochemical, mechanical, and biological methods. The resulting hybrid hydrogels showed enhance mechanical properties and withstood injection without phase disruption. In mice, the hydrogels showed faster and improved self-healing properties compared to their nonmagnetic counterparts. Thanks to these superior physical properties and stability during culture, they can be used as 3D scaffolds for cell growth. Additionally, magnetic short-peptide hydrogels showed good biocompatibility and the absence of toxicity, which together with their enhanced mechanical stability and excellent injectability make them ideal biomaterials for in vivo biomedical applications with minimally invasive surgery. This study presents a new approach to improving the physical and mechanical properties of supramolecular hydrogels by incorporating MNP, which confer structural reinforcement and stability, remote actuation by magnetic fields, and better injectability. Our approach is a potential catalyst for expanding the biomedical applications of supramolecular short-peptide hydrogels.

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Journal of Biomedical Materials Research Part B: Applied BiomaterialsVolume 109, Issue 7 p. 933-937 ISSUE INFORMATIONFree Access Issue Information First published: 13 May 2021 https://doi.org/10.1002/jbm.b.34652AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Volume109, Issue7July 2021Pages 933-937 RelatedInformation