Manufacture Of Products Research Articles

Implementasi E-Katalog IKM Berbasis Website Pada Dinnakerind Di Kabupaten Demak

In this digital era, information technology is very important to develop small and medium industries (SMEs). The Department of Labor and Industry of Demak Regency seeks to improve the competitiveness of SMEs through a website-based e-catalog. The aim of this research is to create and implement an e-ICM catalogue that can be accessed online by the public as well as to evaluate how effective the catalogue is in promoting local products. Needs analysis, system design, implementation, and testing are the measures used in this research. This research aims to design and implement an SMEs e-catalog that can be accessed online by the community, and evaluate its effectiveness in promoting local products. The research method includes needs analysis, system design, implementation, and testing. The result is an e-catalog website that displays complete information about SMEs products in Demak Regency, including product descriptions, images, and manufacturer contacts. This website also has a search feature and product categories to facilitate users. Using the black-box testing method, the test was conducted with a usability survey of 22 respondents, which yielded a score of 86.8%. It is expected that the system will help develop small and medium-sized industries (SMEs) in Demak district, improve product visibility, and support the local economy.

Expanding our food supply: underutilized resources and resilient processing technologies.

Many underutilized food resources have been traditionally used by regional and poor communities. The history of their consumption makes them potential new food sources for incorporation into the wider food supply. The ability to tap the potential of undervalued and underutilized food sources will reduce the world's reliance on a limited number of food sources and improve food security and sustainability. The expansion of the food diversity of the food supply to include underutilized food resources will require overcoming challenges in the efficient and profitable production of the raw material, application of suitable postharvest handling procedures to maintain the quality of perishable produce, and the use of appropriate traditional and emerging food processing technologies for conversion of the raw material into safe, nutritious and consumer-acceptable foods. Improvement of food processing technologies, particularly resource-efficient resilient food processes, are required to ensure the safety, quality and functionality of the whole food or extracts, and to develop ingredient formulations containing new foods for manufacture of consumer food products. Factors that help facilitate the social acceptance of new underutilized foods include increasing consumer knowledge and understanding of the contribution of new underutilized food resources to diet diversity for good nutrition, confidence in the safety and value of new foods, and their low environmental impact and importance for future sustainable food. The introduction of new underutilized food resources will increasingly require collaboration along the whole food value chain, including support from government and industry. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Risk-Based Approach for Defining Retest Dates for Active Pharmaceutical Ingredients and Excipients.

Drug substances and excipients must be stored in recommended storage conditions and should comply with their specifications during the retest period for their use in the manufacture of drug products. The ICH (International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use) and WHO (World Health Organization) regulatory guidelines mandate that after the retest period, the drug substances must be retested for compliance with the specification and then used immediately in the manufacture of the finished product. Although these substances can be retested multiple times, an emphasis is placed on immediate use following a retest and compliance with standards. The phrase "used immediately" is ambiguous and is left for interpretation. In this article, we will look at the various processes that must be completed to determine the retest date. In addition, we present a risk-based method for establishing retest dates and the time during which material can be used.

Effects of l‐arginine/l‐lysine on physicochemical properties of sodium‐reduced Cantonese sausage without exogenous phosphate addition

SummaryThe study inquired the influences of l‐arginine and l‐lysine on the physicochemical properties (including weight loss rate, water activity, texture, colour, lipid and protein oxidation, residual nitrite content and total volatile basic nitrogen (TVB‐N) content) of low‐sodium and low‐phosphate Cantonese sausage during the 1‐ and 7‐day storage. The results showed that the addition of l‐arginine or l‐lysine reduced the weight loss rate, TBARS content, carbonyl group content and residual nitrite level, slightly decreased water activity, and enhanced L*, a* and b* values. Compared to the control, 0.3% l‐arginine or l‐lysine declined hardness and chewiness, while 0.6% l‐arginine or l‐lysine elevated these two parameters. However, the addition of l‐arginine or l‐lysine also led to an increase in TVB‐N content. Overall, l‐arginine and l‐lysine improved the qualities of low‐sodium and low‐phosphate Cantonese sausage, which might be attractive to meat product manufacturers.

Modelling of dynamic mastication physics of cheese/casein gels as function of milk-fat distribution and intensity of thermo-mechanical treatment

Pasta filata-style cheese products are among the world’s most famous cheese varieties. Thermo-mechanical processing of cheese curd results in stringy, fibrous, and anisotropic structures with pleasing texture attributes. A recent area of research focuses on improving yield during the manufacturing of pasta filata-type cheese products by homogenizing the milk. This process reduces the size of fat droplets, leading to better retention of milk fat during curd plasticization. As this sometimes results in texture deficits, this study aims to investigate the impact of thermo-mechanical processing on curd from homogenized and non-homogenized milk. The hypothesis is that increased thermo-mechanical processing, leading to more anisotropic structural elements, may offset texture deficits caused by homogenization. To assess textural and structural changes due to homogenization and thermo-mechanical processing, mechanical tests including rheology and texture analysis were conducted, along with confocal-laser-scanning microscopy. Additionally, sensory evaluation involving panelists consuming the samples and recording mastication properties such as muscle activity and jaw movement was carried out. Dynamic data modeling was used to derive connections between structure and texture. Results showed that homogenization alone did not yield significant differences between the samples, but plasticization and texturization properties differed significantly. Non-homogenized samples developed a distinct fibrous structure, and muscle activities and jaw movements increased significantly (p < 0.01) with longer thermo-mechanical processing.

Analysis and selection of composite material for the manufacture of products during the design preparation stage

Analysis and selection of composite material for the manufacture of products during the design preparation stage

Взаимодействие коммерческих и государственных цифровых платформ: преимущества и эффекты

In the context of digital transformation in economic development, the attention of business entities is largely focused on the processes of exchanging knowledge, skills, and experience between market participants, while the priority is not the production technologies themselves but the processes of managing them. This task of developing information and communication infrastructures has been successfully solved with the help of digital platforms and digital ecosystems created both in business structures and in the fields of public and municipal administration. Multifunctional digital platforms with a complex system of interactions between their various participants, which combine several types of operational activities (development, manufacture of products, including complementary ones, bringing platform products to the consumer, etc.), are the most effective since they form the processes of sharing company assets and joint value creation. Functional differences between commercial and government digital platforms were shown, as were problems arising in this regard in their interaction in the digital environment and ways to solve them. The analysis of the practical use of digital platforms created with government participation, as exemplified by the ecosystems of PJSC Sberbank and PJSC Gazprom, is carried out. During the development of new digital platforms, oligopolistic competition arises in business, which results in the monopolization of network effects and pricing policies by the creators of the platforms. Interaction in the field of digital business between commercial and government structures makes it possible to coordinate these processes and regulate macroeconomic proportions across the country as a whole. The possible effects of the implementation of commercial digital platforms in public administration functions are determined, and the interdependencies of public and private platforms and ecosystems formed on their basis are established. The possible effects of interaction between the state and business in the course of the development of new digital platform solutions and the prospects and advantages of their joint use are revealed. The interaction of the government and business in the course of the development of new digital platform solutions allows for the growth of citizens’ solvency and creates conditions for the growth of the public product of the state as a whole. The growth of public value from the introduction of government digital platforms is essential, as is their interaction with commercial digital platforms.

Total Cost of Ownership of Electric Car and Internal Combustion Engine Car with Performance Normalization

In the transportation sector, motorized vehicles are currently a contributor to pollution in Indonesia, producing CO2 emissions. In an effort to achieve environmentally friendly and low-carbon motorized vehicles, promoting electric cars to the public is an important strategy that is being pursued by countries throughout the world. The large target of converting ICE cars to electric cars requires a series of analyzes to accelerate efforts to convert the use of electric cars from various angles which will ultimately be successful as a promotional medium for potential electric car users. To carry out this analysis, there is one calculation method, namely calculating the total cost of ownership (TCO) of an electric car. This method has been studied in countries that are concerned with promoting the use of electric cars. In this research, we will present a calculation model for the TCO of electric cars and the TCO of conventional internal combustion engine (ICE) cars as a comparison. The cars analyzed in this research consist of several electric cars and ICE cars according to their respective performance levels, where for each electric car an analysis of several battery ownership schemes is also attempted, namely those owned by the car owner, rental batteries and those owned by the car owner. However, some components can be repaired. The calculation model will consist of capital and operational costs, namely the purchase price, resale value, government subsidies, retailer discounts, battery replacement costs, maintenance, insurance, vehicle tax and energy consumption costs. All data was obtained from literature studies and information from vehicle distributors in Indonesia. The TCO model is analyzed based on the average distance traveled, namely 20,000 km/year for 10 years of driving. The research results show that the TCO of BEVs with a rental battery scheme is cheaper than ICEs for cars with 93, 167 &amp; 210 hp, namely 2,961; 6,227; &amp; 5,633 (USD) and the TCO of ICE is cheaper than BEV with a rental battery scheme on cars with 40 &amp; 95 hp, namely 796 &amp; 5,793 USD. Several components that make up TCO costs will later be proposed to be considered for adjustment so that vehicle purchases can grow significantly. Knowledge from this research can be useful for consumers, product manufacturers, planners, and government policy makers.

Innovative concepts in diet therapies in disorders of gut-brain interaction.

Diet therapy in disorders of gut-brain interaction (DGBI) is rapidly advancing, with accumulating evidence to support two innovative therapies-manipulation of dietary fibers and enzyme supplementation-that target specific DGBI pathophysiology and modulate digestion. Dietary fibers escape digestion in the upper gastrointestinal tract and can influence gut function by impacting digestion, improving laxation, and interacting with the microbiota. A more nuanced understanding of different fiber types and their ability to impact gut function in highly specific ways has shown that fibers can impact distinct gut symptoms and pathophysiology. By considering their functional characteristics of bulking, gel-forming, and fermentability, restriction or supplementation of specific fibers can offer clinical value in DGBI. Similarly to fiber specificity, emerging evidence suggests that supplemental digestive enzymes may be targeted to known food triggers with consideration that enzymes are substrate specific. Limited evidence supports use of lactase to target lactose, and α-galactosidase to target galacto-oligosaccharides. Application of enzymes during manufacturing of food products may prove to be an additional strategy, although evidence is scant. Both innovative therapies may be utilized in isolation or in combination with other diet and nondiet therapies. Implementation can be guided by the principles that fiber modulation can be targeted to specific symptomology or requirement for alterations to gut function, and digestive enzymes can be targeted to known food triggers. This review aims to summarize recent literature of these two innovative concepts and provide practical suggestions for their implementation in clinical practice.

CHOICE OF THERMAL INSULATION MATERIAL PRESS FORM FOR MANUFACTURING PRODUCTS FROM COMPOSITE MATERIALS

Purpose. The development of a method of thermal calculation of molds for the manufacture of products from composite materials, which allows you to choose an insulating material that ensures effective savings in heat consumption. Research methods. A theoretical analysis of the existing thermal calculations of the molds was carried out; an analysis of the theory of heat exchange based on the theory of similarity was carried out, using dimensionless criteria of Nusselt, Grashof and Prandtl; an analysis of reference data on the properties of insulating materials was carried out. Results. In the course of the work, the processes of convective heat transfer from the side surface of the mold were clarified and described; a method of calculating energy losses through heat transfer at a certain temperature on the outer surface of an uninsulated mold was developed; it was established that the temperature of the wall, from which the heat transfer is actually carried out, plays a decisive role in the selection of the insulating material; a method of reducing energy consumption is determined by applying a layer of heat-insulating material on the heat transfer surface of the mold, reaching a given temperature on the isolated surface of the mold; on the example of a mold of specific dimensions, the optimal materials were selected and their effectiveness in reducing heat consumption was determined. Scientific novelty. Developed methods for evaluating the effectiveness of heat-insulating material by calculating the temperature on the insulated surface of the mold; in this connection, the reduction of heat loss through heat transfer is achieved with the help of the selected optimal heat-insulating material, which has the appropriate thermophysical and technological properties. Practical value. Information on the impact of the design of the mold matrix and reference information on the thermal conductivity of heat-insulating materials are provided; the proposed calculation method can be used in the design of energy-saving molds for the manufacture of products from composite materials.

Enhancing sustainability in medical product supply chains: The role of remanufacturing and government subsidies

This study explores the impact of government subsidies on the production dynamics within the medical product supply chain, particularly focusing on the remanufacturing of medical goods. Amidst the backdrop of the COVID-19 pandemic, which has underscored the critical shortages in medical supplies, our research delves into the adoption of remanufacturing practices by medical product manufacturers as a strategic response to these shortages and environmental concerns. We investigate how government subsidies influence the production volumes of original manufacturers and remanufacturers and examine the competitive interplay between newly manufactured and remanufactured medical products. Through the development of three production game models—Scenario B (manufacturers produce both new and refurbished products), Scenario N (separate production of new and refurbished products by manufacturers and remanufacturers, respectively), and Scenario C (similar to Scenario N but includes a certification fee paid by remanufacturers to original manufacturers)—we analyze the strategies that could mitigate supply deficiencies during medical crises. Our findings indicate that the certification strategy (Scenario C) not only yields the highest total production of medical products but also offers a viable solution to enhance the sustainability of the entire medical production system by alleviating supply chain disruptions. Furthermore, we discuss the managerial implications of our results, emphasizing the potential of a joint remanufacturing strategy to stabilize the supply chain and foster environmental conservation. Lastly, we highlight our study's limitations and suggest future research directions, particularly concerning the variability in product quality and the reliance on government subsidies. This research contributes to a nuanced understanding of green remanufacturing within the pharmaceutical supply chain, offering insights for manufacturers, remanufacturers, and policymakers aiming for sustainable industry practices.

An ecosystem orchestration framework for the design of digital product passports in a circular economy

AbstractDigital product passports are considered key levers for a circular economy. By encouraging intraorganizational and interorganizational exchange of data and information, they enable informed life cycle decision‐making to circulate products and materials at their highest utility. This is contingent on the engagement of supply chain actors to contextualize the industrial implications in the span between regulatory compliance and additional value‐generation. Through the lens of ecosystem orchestration, this multiple case study draws upon industrial insights from three product manufacturers as well as a sample of their respective suppliers, service partners, customers, and third‐party recycling companies to identify 16 practices and five orchestration mechanisms. These unfold at different stages of ecosystem maturity. Combined, they construct a framework for the adaptation of digital product passports in industrial ecosystems. This provides guidance for practitioners throughout the contextualization and utilization of digital product passports and extends the scientific debate in the direction of operationalization. As digital product passports are still in their infancy, avenues for further research are identified, particularly concerning the operational foundations, including the adherent driving and inhibiting factors toward the implementation of digital product passports.

Влияние технологических факторов на плотность и прочность зологазобетона

One of the promising areas of fly ash utilization is the production of cellular concretes. The experience of using cellular concrete products in construction has shown that they can successfully compete with most traditional materials combining high thermal insulation and structural properties. The paper examines the influence of technological factors: grinding, composition, water temperature and water solid ratio on the density and strength of the gas and gold cement. Purpose of the study. Determination of the influence of technological factors of production on the density and strength of ash gas concrete. Materials and methods. Portland cement, fly ash from Ekibastuz GRES-2, bassanite, and aluminum powder were used as starting materials. The composition of ash gas concrete with an average density of 700, 800 and 900 kg/m3 was developed according to the technical requirements of CH 277-80 – Instructions for the manufacture of cellular concrete products. The physical and mechanical properties of cellular concrete were determined in accordance with the technical requirements of the current standards. The result s and discussion. On the basis of the basic composition, the influence of technological factors on the density and strength of ash gas concrete has been studied. As a result of the research, it was found that the strength of samples with an average density of 700 kg/m3 reaches 3.5 MPa, and with an average density of 800 and 900 kg/m3 – 5.1 and 7.6 MPa, respectively. These indicators confirm that the samples obtained from mechanically activated gold cement binder according to the technical requirements of GOST 25485-2019 correspond to the structural and thermal insulation properties of cellular concrete. Resume. The physical and technical properties of raw materials, concrete mixtures and cellular concretes were studied using Ekibastuz GRES-2 ash as a fine filler. The study showed that production technological factors such as the dispersion and composition of the mixture, the water-solid ratio and the temperature of the mixing water have a significant effect on the density and strength of the ash gas concrete. Optimization of these parameters makes it possible to achieve the specified values of density and strength in the resulting material. Proposals for practical application and direction of future research. The possibility and expediency of using the ash of Ekibastuz GRES-2 as an additive in the manufacture of ash gas concrete has been established.

Research on Decision Optimization of Supply Chain Quality Information Disclosure Considering Stigma Level

With the frequent occurrence of online public opinion events, the problem of product stigma is becoming increasingly serious. Enterprises must use effective quality information disclosure strategies to reduce losses affecting market sales and profit. Therefore, this paper aims to address the supply chain structure composed of one product manufacturer and one component manufacturer under the influence of stigma. It constructs a decision optimization model under three scenarios: no information disclosure, the product manufacturer disclosures information, and the component manufacturer disclosures information, and uses Stackelberg game theory to solve and analyze the model. Furthermore, we use numerical examples to verify the model results, and provide management suggestions for enterprises. The research results show that enterprises suffering from product stigma should actively implement information disclosure strategies to reduce their profit losses, and the lower the stigma level, the better the effect of information disclosure will be; when the stigma level becomes more serious, enterprises should take timely steps to reduce the sales price of products, the sales price of components, and the efforts to disclose information; for industries that value confidentiality of product information, although the implementation of information disclosure by the component manufacturer can require less effort for information disclosure, the two enterprises will suffer higher economic losses.

Assessment of neuro-pulmonary crosstalk in asthmatic mice: effects of DiNP exposure on cellular respiration, mitochondrial oxidative status and apoptotic signaling

Human health is becoming concerned about exposure to endocrine disrupting chemicals (EDCs) emanating from plastic, such as phthalates, which are industrially employed as plasticizers in the manufacturing of plastic products. Due to some toxicity concerns, di(2-ethylhexyl) phthalate (DEHP) was replaced by diisononyl phthalate (DiNP). Recent data, however, highlights the potential of DiNP to interfere with the endocrine system and influence allergic responses. Asthma affects brain function through hypoxia, systemic inflammation, oxidative stress, and sleep disturbances and its effective management is crucial for maintaining respiratory and brain health. Therefore, in DiNP-induced asthmatic mice, this study investigated possible crosstalk between the lungs and the brain inducing perturbations in neural mitochondrial antioxidant status, inflammation biomarkers, energy metabolizing enzymes, and apoptotic indicators. To achieve this, twelve (n = 12, 20–30 g) male BALB/c mice were divided into two (2) experimental groups, each with five (6) mice. Mice in group II were subjected to 50 mg/kg body weight (BW) DiNP (Intraperitoneal and intranasal), while group I served as the control group for 24 days. The effects of DiNP on neural energy metabolizing enzymes (Hexokinase, Aldolase, NADase, Lactate dehydrogenase, Complex I, II, II & IV), biomarkers of inflammation (Nitric oxide, Myeloperoxidase), oxidative stress (malondialdehyde), antioxidants (catalase, glutathione-S-transferase, and reduced glutathione), oncogenic and apoptotic factors (p53, K-ras, Bcl, etc.), and brain histopathology were investigated. DiNP-induced asthmatic mice have significantly (p < 0.05) altered neural energy metabolizing capacities due to disruption of activities of enzymes of glycolytic and oxidative phosphorylation. Other responses include significant inflammation, oxidative distress, decreased antioxidant status, altered oncogenic-apoptotic factors level and neural degeneration (as shown in hematoxylin and eosin-stained brain sections) relative to control. Current findings suggest that neural histoarchitecture, energy metabolizing potentials, inflammation, oncogenic and apoptotic factors, and mitochondrial antioxidant status may be impaired and altered in DiNP-induced asthmatic mice suggesting a pivotal crosstalk between the two intricate organs (lungs and brain).

Formation of Environmental Culture of Younger School Students in the Content of Technological Education

The article examines the problem of environmental education of the younger generation, in particular, focuses on the importance of the formation of the environmental culture of younger schoolchildren, and reviews scientific research on the issues of environmental culture of the individual and the features of its formation at the younger school age. It is noted that the formation of the ecological culture of younger schoolchildren can take place through the study of educational material of the technological educational field, in particular, ecologically oriented content. The author examines the importance of using secondary materials for the manufacture of products in design and technology classes, offers the topic of environmental projects aimed at increasing students' environmental awareness and their desire to protect the environment. The article offers topics of practical works that help students better understand the impact of their actions on nature and educate them in a responsible attitude towards the environment. The author provides recommendations to teachers on the use of effective teaching methods aimed at forming the ecological culture of junior high school students in design and technology lessons.

Support medium development for 3D printing natural rubber latex via direct ink writing in the support bath technique

The direct manufacturing of rubber products from natural rubber latex through 3D printing, particularly extrusion in air, faces challenges in creating intricate shapes. Research suggests that utilizing 3D printing with extrusion in a support medium, known as direct ink writing (DIW), is effective for crafting complex-shaped rubber products. However, few studies have explored 3D printing natural rubber as a support medium. This study focuses on formulating a support medium for the DIW printing of natural rubber by incorporating triethanolamine (TEA) and alcohol in varying proportions. Key characteristics, such as viscosity, were assessed for each formulation, along with essential printing parameters, such as speed and flow rate. A suitable support liquid consisting of TEA (2.5 g), alcohol (160 g), Carbopol (1.5 g), and water (200 g) was determined for DIW printing natural rubber. The optimal settings were determined to be a nozzle size of 0.85 mm, a speed of 30 mm/s, and a flow rate of 30 mm3/s. Comparative results from the forming process indicate that 3D-printed rubber specimens exhibit poorer mechanical properties than traditionally molded specimens, owing to material uniformity. The vulcanized rubber system with the EV pattern exhibited superior mechanical characteristics. The developed support medium for DIW printing shows potential for use in intricate natural rubber products; however, further exploration of additional parameters is crucial for advancing complex-shaped natural rubber manufacturing using 3D printers.

Analysis of Energy and Material Consumption for the Manufacturing of an Aeronautical Tooling: An Experimental Comparison between Pure Machining and Big Area Additive Manufacturing.

Additive manufacturing (AM) has been fully incorporated into both the academic and the industrial world. This technology has been shown to lower costs and environmental impacts. Moreover, AM-based technologies, such as wire arc additive manufacturing (WAAM), have been proven suitable for the manufacturing of large products with significant mechanical requirements. This study examines the manufacture of two aeronautical toolings: first, using conventional techniques, and second, using a big area additive manufacturing (BAAM) process, specifically WAAM technology, followed by second-stage hybrid machining. Both toolings can be considered interchangeable in terms of design and performance. Energy and material consumption were analysed and compared throughout both tooling procedures. The results show the important optimisation of both procedures in manufacturing WAAM tooling, encompassing the additive process and second-stage hybrid machining. Nevertheless, the time required for WAAM tooling manufacturing increased significantly compared to conventional manufacturing tooling. Moreover, based on metrology data from the AM process, a theoretical study was conducted to assess different design optimisations for WAAM tooling manufacturing and determine their influence on material and energy consumption. These theoretical results improve those already obtained regarding energy and raw material savings.

ENERGY CROPS – OUTLOOK FOR ENVIRONMENTAL FUTURE FOR HUMANITY AND THE PLANET EARTH

The growing risk of irreversible depletion of fossil energy sources like petrol, natural gas and coal, and the emissions of gasses consequence from their combustion, is causing the necessity of the introduction of energy crops for the manufacture of energy products. These include annual or perennial plants, fast-growing tree types – hybrid willows, poplars and others, and water crops – red algae, giant kelp, seaweed etc. Most preferred in Europe and the US are Paulownia sp. (P. elongata и P. tomentosa), giant reed (Arundo donax), elephant grass (Miscanthus giganteus), weeping willow (Salix babylonica) and wicker (Salix viminalis). So far in Bulgaria are preferred herbaceous energy crops such as corn, canola, sorghum, etc., but also were introduced some kind of timber. The global development of the sector would help to address the environmental problems associated with the release of huge quantities of greenhouse gasses. In the processing and utilization of energy crops, the amount of waste products is reduced to near zero, thereby avoiding environmental pollution.

Adeno-associated virus vector-based gene therapy for hereditary diseases: current problems of application and approaches to solve them

INTRODICTION. Currently, gene therapy based on adeno-associated virus (AAV) vectors faces a number of barriers, both biomedical and technological, which require studying and overcoming for further development of this gene therapy technology.AIM. This study aimed to analyse the use of gene therapy for a range of hereditary diseases, taking into account the barriers associated with its side effects and insufficient efficacy, the determination of the therapeutic window, and individual characteristics relevant to a particular hereditary disease; additionally, the study aimed to review the approaches to lifting these barriers and increasing the availability of gene therapy through the improvement of technological approaches to production and the reduction of production costs.DISCUSSION. The authors reviewed the experience accumulated for gene therapy products that were approved or undergoing clinical trials. The study included a gene therapy applicability assessment using several hereditary diseases as a case study. The assessment showed that correct determination of the therapeutic window for a medicinal product and timely diagnosis of a hereditary disease were essential for effective and safe gene therapy. The study considered the strategies used to reduce the risks of adverse events and increase the effectiveness of AAVbased gene therapy. The authors assessed technological advancements in the manufacturing of AAV-based gene therapy products. The most perspective directions were the transition to suspension culture systems, the improvement of bioreactors, the use of new methods and materials for the purification of viral particles, the improvement of transfection systems, and the creation of new host cell lines. Ultimately, this can lead to lower production costs and an increased availability of gene therapy.CONCLUSION. Currently, gene therapy is used only for a small range of hereditary diseases. Significant barriers to its use are due to insufficient efficacy, risks of adverse events, and high costs for treatment. Ongoing biomedical and technological development should lift many of these barriers and increase access to gene therapy.