The normal anatomy of mandibular canines presents with 1 root and 1 root canal. Two roots are found in approx. 2% of cases, and a bilateral configuration is even rarer. Canines with 2 root canals are found in around 15% of cases. Cone-beam computed tomography (CBCT) enables the detailed visualization of the teeth. The present study aimed to evaluate the prevalence of two-rooted mandibular canines and one-rooted mandibular canines with 2 root canals in a Polish population by using CBCT. A total of 300 consecutive CBCT scans, taken for different clinical indications, were examined to assess permanent mandibular canine anatomy. The study group included 182 females and 118 males aged 12-86 years (mean age: 31.7 years). Among 600 cases, 27 two-rooted teeth were found (4.5%), and there were only 6 cases of onerooted mandibular canines with 2 root canals (1.0%). Six cases of two-rooted canines had this configuration bilaterally, all in females. Five cases of canines with 2 root canals were found on the left side (83.3%). The predominance of the occurrence of two-rooted canines in females (81.5%) was strongly emphasized. The prevalence of two-rooted mandibular canines in a Polish population, evaluated by means of CBCT, was higher, while the presence of 2 root canals was lower than in recent literature reports. There was no side predilection of two-rooted mandibular canines, although their occurrence was higher in females.