Male Population Research Articles

Rehabilitating a 44-year-old Chronically Traumatized Parapalegic Woman: a Case Study

Spinal cord injury (SCI) resulting from trauma imposes major and permanent life changes on the predominantly young male population who receive these injuries. Paralysis of the muscle below the level of injury can lead to limited and altered mobility, self-care and ability to participate in valued social activities. In addition to the musculoskeletal system, many other body systems are also impaired. The psychosocial impact of the SCI can be just as great as the physical impact. Rehabilitation is an important element toward achieving a fulfilling and active life after an SCI. Physical therapist play a key role in rehabilitation process. A detailed neurological assessment of a patient was done and the severity of the injury was graded according to ASIA impairment scale. A systematic treatment plan was made and altered according to the patient’s recovery and needs.

Sensitivity of magnetic resonance elastography to extracellular matrix and cell motility in human prostate cancer cell line-derived xenograft models

Prostate cancer (PCa) is a significant health problem in the male population of the Western world. Magnetic resonance elastography (MRE), an emerging medical imaging technique sensitive to mechanical properties of biological tissues, detects PCa based on abnormally high stiffness and viscosity values. Yet, the origin of these changes in tissue properties and how they correlate with histopathological markers and tumor aggressiveness are largely unknown, hindering the use of tumor biomechanical properties for establishing a noninvasive PCa staging system.To infer the contributions of extracellular matrix (ECM) components and cell motility, we investigated fresh tissue specimens from two PCa xenograft mouse models, PC3 and LNCaP, using magnetic resonance elastography (MRE), diffusion-weighted imaging (DWI), quantitative histology, and nuclear shape analysis. Increased tumor stiffness and impaired water diffusion were observed to be associated with collagen and elastin accumulation and decreased cell motility. Overall, LNCaP, while more representative of clinical PCa than PC3, accumulated fewer ECM components, induced less restriction of water diffusion, and exhibited increased cell motility, resulting in overall softer and less viscous properties. Taken together, our results suggest that prostate tumor stiffness increases with ECM accumulation and cell adhesion - characteristics that influence critical biological processes of cancer development.MRE paired with DWI provides a powerful set of imaging markers that can potentially predict prostate tumor development from benign masses to aggressive malignancies in patients. Statement of significanceXenograft models of human prostate tumor cell lines, allowing correlation of microstructure-sensitive biophysical imaging parameters with quantitative histological methods, can be investigated to identify hallmarks of cancer.

Toward clinical exomes in diagnostics and management of male infertility

Infertility, affecting ∼10% of men, is predominantly caused by primary spermatogenic failure (SPGF). We screened likely pathogenic and pathogenic (LP/P) variants in 638 candidate genes for male infertility in 521 individuals presenting idiopathic SPGF and 323 normozoospermic men in the ESTAND cohort. Molecular diagnosis was reached for 64 men with SPGF (12%), with findings in 39 genes (6%). The yield did not differ significantly between the subgroups with azoospermia (20/185, 11%), oligozoospermia (18/181, 10%), and primary cryptorchidism with SPGF (26/155, 17%). Notably, 19 of 64 LP/P variants (30%) identified in 28 subjects represented recurrent findings in this study and/or with other male infertility cohorts. NR5A1 was the most frequently affected gene, with seven LP/P variants in six SPGF-affected men and two normozoospermic men. The link to SPGF was validated for recently proposed candidate genes ACTRT1, ASZ1, GLUD2, GREB1L, LEO1, RBM5, ROS1, and TGIF2LY. Heterozygous truncating variants in BNC1, reported in female infertility, emerged as plausible causes of severe oligozoospermia. Data suggested that several infertile men may present congenital conditions with less pronounced or pleiotropic phenotypes affecting the development and function of the reproductive system. Genes regulating the hypothalamic-pituitary-gonadal axis were affected in >30% of subjects with LP/P variants. Six individuals had more than one LP/P variant, including five with two findings from the gene panel. A 4-fold increased prevalence of cancer was observed in men with genetic infertility compared to the general male population (8% vs. 2%; p= 4.4×10-3). Expanding genetic testing in andrology will contribute to the multidisciplinary management of SPGF.

Repeated sexual intercourse as a coping strategy for men with premature ejaculation.

Patients with premature ejaculation (PE) are often concerned and distressed about their sexual performance. Hence, they may be more willing to exploit their refractory period to employ sexual coping strategies in order to improve their unsatisfactory sexual intercourse compared with patients without PE. The study sought to verify the sexual coping strategies of patients with PE in the daily sexual activities. We included both patients with PE and individuals without PE and analyzed their sexual behaviors and attitudes by means of detailed interviews and questionnaires. The main outcomes were perceived intravaginal ejaculatory latency time recording, Premature Ejaculation Diagnostic Tool score, and sexual frequency, attitudes, and behavior log. A total of 182 young patients with PE (age 31.2 ± 6.2 years) and 92 individuals without PE (age 30.7 ± 5.1 years) were included in the study. A total of 53.3% of patients with PE vs 17.4% of individuals without PE reported engaging in multiple sexual intercourse sessions within a single day in the past 4 weeks. PE patients who engaged in multiple intercourse sessions displayed better performance during the second attempt but performed poorly compared with individuals without PE. Scores for the first attempt in PE vs second attempt in individuals with PE vs without PE were the following: intravaginal ejaculatory latency time, 2.4 ± 1.6 vs 4.8 ± 5.7 vs 9.9 ± 9.4 (P < .001); Premature Ejaculation Diagnostic Tool, 14.9 ± 3.1 vs 12.7 ± 4.8 vs 5.2 ± 2.5 (P < .001); satisfaction, 2.9 ± 1.0 vs 3.1 ± 0.8 vs 3.7 ± 1.4 (P < .001). A total of 57.1% of patients held a negative attitude toward precoital masturbation, for reasons such as a reduced sexual desire (21.2%), the belief that masturbation is harmful (17.6%), concerns about erectile function (15.7%), fatigue (9.8%), and other mixed reasons (35.3%). Engaging in multiple intercourse sessions within a day is more common among the young PE population, and using precoital masturbation as a coping strategy is not universally applicable among patients with PE. This is the first study to explore symptom-coping strategies in patients with PE compared with individuals without PE. However, the conclusions cannot be generalized to the entire male population. Patients with PE, compared with individuals without PE, are more inclined to engage in multiple sexual intercourse sessions within a single sexual session, likely in an attempt to compensate for their first unsatisfactory sexual encounter. Moreover, the majority of patients with PE here studied hold a negative attitude toward using precoital masturbation as a coping strategy for symptoms.

Effect of neuromuscular injury prevention strategies on injury rates in adolescent males playing sport: a systematic review protocol.

This review will assess the effectiveness of neuromuscular injury prevention strategies on injury rates among adolescent males playing sports. Adolescent athletes are predisposed to injuries during this period of growth. Growth-related injury risk factors can be mitigated by implementing appropriate neuromuscular injury prevention strategies. This review will include all sporting disciplines in summarizing the components and assessing the effectiveness of injury prevention strategies in the adolescent male population. Randomized controlled trials investigating adolescent males, between the ages of 13 and 18 years, participating in organized sports, in any setting and level of participation, will be included. Studies that evaluate neuromuscular injury prevention strategies (eg, balance, proprioceptive, plyometric, agility, strength, weight, conditioning and sport-specific exercises and training, warm up, cool down, stretches, neuromuscular control) vs no intervention or standard training and competition exposure will be included. The outcomes of interest are injury incidence and prevalence rates. Databases searched will include MEDLINE (PubMed), CINAHL Complete (EBSCOhost), ClinicalKey, SPORTDiscus (EBSCOhost), Physiotherapy Evidence Database (PEDro), Scopus, ScienceDirect, MasterFILE Premier (EBSCOhost), Academic Search Complete (EBSCOhost), Cochrane Central Register of Controlled Trials (CENTRAL) and ClinicalTrials.gov. Gray literature and unpublished studies will be searched via Health and Medical Complete (ProQuest Dissertations & Theses). Study screening and selection against inclusion criteria will be performed. Data extraction and critical appraisal will be performed using the standardized JBI templates and checklists for qualitative research. All stages will be performed by 2 independent reviewers, with conflicts resolved by a third reviewer. PROSPERO CRD42022327047.

A Horizontal and Longitudinal Study on the Changes of Aging Thyroid Function in Elderly Male Population.

There are few follow-up studies on thyroid function in the same group for many years. Therefore, the purpose of this study was to retrospectively analyze the changes of thyroid function in a group of people for 8 years and to explore the changes of thyroid function in elderly men with normal thyroid function with age. Reviewing the records of elderly men who underwent physical examination in the Beijing Hospital physical examination center from 2013 to 2020, 354 subjects were included in the study. According to age, they are divided into 4 groups. The differences in thyrotropin (TSH), anti-triiodothyronine (rT3), free triiodothyronine (FT3), and free thyroid hormone (FT4) among different age groups in initial time (2013) were compared. Longitudinal comparison of changes of thyroid function in the same age group for 8 years was compared too. At the initial time, age was negatively correlated with FT3 (r = 0.349, p < 0.001), positively correlated with rT3 and TSH (r = 0.182, p < 0.001, r = 0.212, p < 0.001), but not correlated with FT4. The results of eight years of analysis show that, for TSH, during the whole follow-up period, the TSH of the >80 years group was higher than that of the <60 years and 60-69 years groups, and the difference was statistically significant. The 70-79 age group was higher than the <60 years group at different time points, except for the age group <60 years. The other three groups showed an increasing trend with age, especially in the group of ≥80 years. For FT3, in 2013, the age ≥80 years group was significantly lower than that of the 70-79 years, 60-69 years, and <60 years old groups (p < 0.05). The analysis results at different time points in each age group showed a downward trend and then an upward trend. For FT4, there was no significant difference in FT4 among different age groups in 2013. Still, during the follow-up period, the age group ≥80 was lower than other age groups in 2019 and lower than the <60 years groups in 2014, 2015, 2019, and 2020, and the difference was statistically significant. The change rule of FT4 with the increase of age was not clear. For rT3, during the whole follow-up period, the rT3 of the >80 years group was higher than that of the <60 years and 60-69 years groups, and the difference was statistically significant. The analysis results at different time points in each age group showed a trend of rising first, then falling, and finally rising. After 2017, the rT3 of the 70-79 years and ≥80 years groups increased with age. The thyroid function index of elderly men changes with age. In transverse analysis, the value of TSH is the highest, and FT3 is the lowest in the group ≥80 years old. There are differences between the changes in the longitudinal analysis and the results of the horizontal analysis. Therefore, the law of thyroid function changing with age in different individuals is not the same as that of the same individual with age, which should be paid more attention in medical research and clinical diagnosis and treatment.

Организационные и клинические факторы выживаемости пациентов при раке предстательной железы в Свердловской области.

Prostate cancer is included in the list of the most common forms of malignant neoplasms in men. The survival rate of the patients with prostate cancer depends on clinical and organizational factors. The network of express-diagnostic urological offices (EDUO) has been deployed in medical organizations of the Sverdlovsk region, designed for early diagnosis of urological and oncourological diseases, and determination of patient routing schemes. The purpose of the study: to determine the organizational and clinical factors of prostate cancer survival based on the data form the Sverdlovsk regional segment of the Russian oncological information system “ONCOR». Materials and methods. Data on all the 20087 newly identified cases of prostate cancer in the Sverdlovsk region in 2005–2022 were exported from the ONCOR database. One- and five-year tumor-specific survival rates were estimated by the life tables. The cumulative survival function was calculated by the Kaplan-Meyer method. Simple and multiple Cox regression analysis was used to find the relative risks of death of patients with prostate cancer depending on the clinical and organizational prognosis factors. Results. In 2005–2022, in the Sverdlovsk region, the level of primary morbidity rate of the male population with prostate cancer increased by +445.8%; 42.3% – 49.5% of cases were newly identified on the III and IV stages depending on the patient’s place of residence. The five–year tumor–specific survival rate of patients with I stage of prostate cancer was 87.4%, II stage – 77.4%, at the third III stage – 65.1%, IV stage – 23.6% (p &lt; 0.001). The five–year tumor–specific survival rate of patients with prostate cancer confirmed in EDUO was 78.8%, in other medical organizations – 57.7%. The relative risk of death of the patient with prostate cancer depended on the place of residence, age, stage of the disease, as well as the availability of EDUO in the regional medical organization. Findings The results of the study confirm the effectiveness of the oncourological care model implemented in the Sverdlovsk region for patients with the prostate cancer.

Epidemiology of Spinal Trauma and Related Complication.

Traumatic spinal injury is leading cause of mortality and morbidity among the people of productive age group. This study aim to find the cause of spinal injury, site ,and mode of injury, treatment option given so that a preventive measures and create awareness among people of this region. This is a prospective observation study done in Karnali Academy of Health Sciences from December 2021 to January 2023. Performa was filled to collect data. Data were entered in excel sheet and transported to SPSS 16.0 and statistical analysis was done . Out of 117 patients male population had higher incidence of spinal trauma (69.2%) with average age 43.9 years . Fall injury was the commonest mode of injury. 65.8% had injury at the thoracolumbar junction followed by lumbar, thoracic and cervical respectively. Cervical injury patients had higher incidence of neurological deficit. The average duration of trauma to hospital presentation was 10.9±11.2 hours. 19.7 % were operated and 6.8 % of patients were referred to other center. Fall injury being the commonest mode of injury in this art of world, prevention and awareness should be raised about the spinal trauma and its consequences. Adequate equipment with health facilities to the distant hospital may reduce the referral rate and duration of presentation to the hospital which ultimately prevent the further damage to the cord.

Epidemiology of Stress Fracture in Police Trainees.

Military recruits and athletes have high occurrence of stress fractures, with very high incidence among military recruits. Symptomatic stress fractures can be disabling in some people. This study aims at identifying pattern and distribution of such fractures in Nepal Police trainee and assess the risk factors and demographics that may help to develop the local guidelines. This study analysed 65 police trainees who presented to the orthopaedic Out Patient Department at Nepal Police and Province Police Hospital diagnosed as a case of stress fracture from 29 December, 2020 to 29 December, 2021. Ethical approval was obtained and different variables analysed were age, sex, Body Mass Index, location of fracture, duration of pain, method of treatment, time to heal the fracture and time to pain free mobilisation of patient. There were 65 trainees with 86 sites of fracture at different bones with 50.8% (33) of male population. The majority of fracture was located at tibia (58.1%) followed by pubic rami (33.7%) with potentially debilitating fracture neck of femur seen in 4.6% subjects. Mean duration of pain was 20.7±14.2 days with mean time for pain free mobilisation 42.2±17.7 days after presentation. 96.5% of those recovered with conservative treatment (activity restriction or cast). The location of stress fracture depends upon the type of training or activities. Modification of activities in early phase of training with early visit for medical care in case of trainees with increasing pain may decrease morbidities and complications requiring operative treatment.

Exposure to benzotriazoles and benzothiazoles in Czech male population and its associations with biomarkers of liver function, serum lipids and oxidative stress.

Benzotriazoles and benzothiazoles (BTs) are high-production volume chemicals as well as widely distributed emerging pollutants with potential health risk. However, information about human exposure to BTs and associated health outcomes is limited. We aimed to characterise exposure to BTs among Czech men, including possible occupational exposure among firefighters, its predictors, and its associations with liver function, serum lipids and oxidative stress. 165participants (including 110 firefighters) provided urine and blood samples that were used to quantify the urinary levels of 8 BTs (high-performance liquid chromatography-tandem mass spectrometry), and 4 liver enzymes, cholesterol, low-density lipoprotein, and 8-hydroxy-2'-deoxyguanosine. Linear regression was used to assess associations with population characteristics and biomarkers of liver function, serum lipids and oxidative stress. Regression models were adjusted for potential confounding variables and false discovery rate procedure was applied to account for multiplicity. The BTs ranged from undetected up to 46.8ng/mL. 2-hydroxy-benzothiazole was the most predominant compound (detection frequency 83%; median 1.95ng/mL). 1-methyl-benzotriazole (1M-BTR) was measured in human samples for the first time, with a detection frequency 77% and median 1.75ng/mL. Professional firefighters had lower urinary 1M-BTR compared to non-firefighters. Urinary 1M-BTR was associated with levels of γ-glutamyl transferase (β = -17.54%; 95% CI: -26.127,-7.962). This is the first study to investigate BT exposure in Central Europe, including potentially exposed firefighters. The findings showed a high prevalence of BTs in the study population, the relevance of 1M-BTR as a new biomarker of exposure, and an urgent need for further research into associated adverse health outcomes.

Use of Community Health Workers Among U.S. Male Latino Population: A Scoping Review.

Community Health Workers (CHWs) are an effective strategy to address the health needs of specified communities. The purpose of this scoping review was to explore the use of CHWs to address the health needs of the Latino male population. This project used specific search terms to identify relevant articles from PubMed, PsycINFO, and Google Scholar. Eligible articles included studies, conducted in the U.S. and in English from 2010 to 2022, that assessed the use of CHWs among a predominantly male (≥ 50%) Latino population. Twenty articles consisting of 13 interventions were identified for review and were further categorized into male-only participant samples (n = 10) and mixed-gender but predominantly-male participant samples (n = 10) for synthesis. Male-only participant samples focused on occupational health and participant's social support and networks. Predominantly-male, mixed-gender participant sample interventions were disease-centered and emphasized the longitudinal support of CHWs as well as social networks. Of the 13 interventions reviewed, almost all (n = 10) utilized existing social networks for engagement and nearly half (n = 6) employed community-based participatory research (CBPR) principles. Findings suggest that recruiting CHWs from within existing social networks and using CBPR are important factors for successful health promotion among male Latinos.

THE COVID-19 PANDEMIC IN THE PLEVEN REGION FOR A THREE-YEAR PERIOD (MARCH 2020 – MARCH 2023)

The COVID-19 pandemic had a major impact on the capacity of health systems to continue the delivery of essential health services. While health systems were being challenged by increasing demand for care of COVID-19 patients, it was critical to maintain preventive and curative services, especially for vulnerable populations. Materials and methods: A retrospective analysis was conducted on epidemiological and demographic characteristics of the COVID-19 pandemic in the Pleven region (March 2020 – March 29 2023). Data were obtained from annual reports of the National Statistical Institute and annual epidemiological analyses of Regional Health Inspectorate–Pleven and were analyzed using statistical software (IВМ SPSS Statistics 19.0). Results: There were 36,308 confirmed cases of COVID-19 (medical workers – 2,176 confirmed cases), with 1,895 deaths. The cases during the years were 5,646 (2020), 16,258 (2021), 15,225 (2022). The incidence of COVID-19 in the region was 2,389; 6,964, and 6,668 at 100,000 populations in 2020, 2021 and 2022, respectively. The prevalence of the disease was 93%, 98% and 94% of all registered respiratory transmitted cases (p&lt;0.0005). There were four waves with the highest incidence (November 2020, March 2021, October 2021, and January 2022, in concordance with registered for Bulgaria). The highest mortality of COVID-19 (15% of deaths) was registered during 2021 – 456 and 368 at 100,000 population for males and females, respectively (16% and 15% of deaths, respectively; 81% – at ages 65+). Conclusion: The global implementation of different interventions had a significant impact on slowing down the community transmission of SARS-CoV-2. Health care and resources must be focused on the vulnerable populations.

Polypill as a means of population pharmacological prevention of cardiovascular diseases

Non-communicable diseases, among which arterial hypertension and ischemic heart disease are the predominant contributors to disability and mortality, have long been identified as a strategic target of preventive intervention in our country. The situation is aggravated by covid-19 associated mortality, which is characterized by delayed dynamics and is largely related to cardiovascular. Excess mortality, in the structure of which cardiovascular diseases are leading, requires new solutions. One of them is population pharmacoprevention, based on the use of complex fixed combinations of drugs targeting different risk factors, as arterial hypertension and dyslipidemia, as most important. This concept, which logically follows from modern realities, is in contradiction with the existing approach of personalized therapy in high/very high risk groups, because of its complexity, leading to patients noncompliance. The new system of cardiovascular risk stratification SCORE 2 introduced in the Russian Federation significantly contributes to the spread of the concept of polypills as a unified approach primarily in the most vulnerable population of males 50 years and older.

Serial mediating effects of childhood trauma and conduct behaviors on the impact of family history among patients with alcohol use disorder

Family history (FH) of alcoholism increases the risk of alcohol use disorder (AUD); however, the contribution of childhood trauma (CT) in this respect remains unclear. This study investigated the relationship between FH and AUD-related clinical characteristics (social onset, antisocial tendency, and severity of problematic alcohol consumption) through the mediating effects of childhood trauma (CT) and conduct behaviors (CB) in a Korean male population with AUD. A total of 304 patients hospitalized for AUD at 16 psychiatric hospitals completed standardized questionnaires, including self-rated scales. Mediation analyses were performed using the SPSS macro PROCESS. Individuals with positive FH (133, 44%) had greater CT and CB and more severe AUD-related clinical characteristics than those without FH (171, 56%). In the present serial mediation model, FH had significant direct and indirect effects on AUD-related clinical characteristics through CT and CB. Indirect effects were 21.3% for social onset, 46.3%, antisocial tendency, and 37.9% for problematic drinking. FH directly contributed to AUD-related clinical characteristics, and CT and CB played mediating roles. This highlights the importance of careful intervention and surveillance of adverse childhood experiences and conduct disorder to prevent and mitigate alcohol-related problems in individuals with FH of AUD.

Isolation, identification, and molecular characterization of Hepatitis B and C viruses in patients from Bahawalpur, Pakistan

HBV and HCV are globally linked to chronic liver diseases and spread mainly through parenteral, vertical, and sexual routes. The epidemiologies of HBV and HCV infections in Bahawalpur, Pakistan was poorly understood. This study aimed to evaluate epidemiological dynamics and risk factors in Bahawalpur, Pakistan. A total of 263 samples were detected with HCV and HBV among various age groups between 21-80 years via molecular techniques (ELISA and PCR). Results for disease prevalence showed a higher rate for HCV as compared to HBV. Further, the gender-wise prevalence of HBV and HCV was also assessed. PCR revealed 50.1% prevalence rate in the male population as compared to 46% in females whereas, ELISA measured 34.5% and 17.4%. Moreover, results also confirmed the higher infection rate of Hepatitis in males with moderate age as compared to young and older age. This study concluded a higher prevalence rate of hepatitis C in the population of Bahawalpur, having moderate age and causing a huge loss of economy to the community as well as Pakistan. Our findings improve understanding of HBV and HCV infection prevalence in Bahawalpur City, aiding government efforts to minimize associated losses.

Length frequency distribution, length-weight relationship and condition factors of Hilsa Shad, Tenualosa ilisha from Bangladesh

Hilsa shad, Tenualosa ilisha, a cherished and economically vital species, thrives in the waters of Bangladesh. It holds a prominent role in bolstering the nation's food security and influencing the ecological dynamics of aquatic ecosystems along the Bangladeshi coast and estuaries. This comprehensive study encompasses data collection efforts on the Hilsa fish species, conducted at 12 commercial landing centers throughout Bangladesh over the course of 2020. The study describes some fundamental components, including length frequency distribution, length-weight relationships, and condition factors, offers invaluable insights into the growth, health, and overall well-being of Hilsa population. Notably, the length-weight relationship analysis revealed size ranges of 11.5-59.2 cm and 11.0-49.0 cm total length for females and males, respectively. The observed b values indicated positive allometric growth (b &gt; 3.00) for female population and negative allometric growth (b &lt; 3.00) for male population in the length-weight relationship. Among the condition factors scrutinized, Fulton's condition factor emerged as the most reliable indicator for assessing the health and condition of the Hilsa population. This study offers valuable insights for biologists, aiding in the assessment of the species' status and providing essential information for fishery biologists and conservation biologists. Such insights are instrumental in the management and ultimate conservation of this iconic species in Bangladesh.

Production of fertile YY pseudofemales in Nile tilapia with LG23 as the sex chromosome

Nile tilapia display conspicuous differences in growth rates between genders, as males tend to outpace and outgrow females. The production of all male Nile tilapia populations using genetically male tilapia (GMT) technology represents the most efficient and scientifically grounded approach. Most of the Nile tilapia stocks in aquaculture use LG23 as sex chromosome. The crux of GMT lies in the acquisition of YY supermales and YY pseudofemales (pF). Nonetheless, the utilization of GMT is impeded by the lower fertility and even infertility of feminized YY-pF. In this study, we found that YY-pF exhibited male-like genital papillae, and exhibited significantly higher androgen levels and lower E2 levels compared with that of fertile XY-pF. YY-pF carries two copies of Amhy but lacks Amh, and XY-pF possesses one Amhy and one Amh. In XY-pF, the expression of Amh/Amhy was only observed in granulosa cells of phase I and II follicles, while in YY-pF, Amhy expression persisted from phase I to IV. On the contrary, Cyp19a1a was found to be highly expressed in granulosa cells of phase III and IV follicles in XY-pF, but significantly reduced in granulosa cells of phase III and IV follicles in YY-pF, exhibiting a reciprocal expression pattern with Amhy. Consistently, YY-pF with one copy of Amhy mutation (YY-Amhy+/−-pF) were fertile. These results indicated that prolonged expression of Amhy led to a decrease in the expression of Cyp19a1a, followed by a decrease in serum E2 levels and an increase in androgen levels. Based on these data, we treated YY-pF with both E2 and trilostane (androgen synthetase inhibitors) simultaneously at the vitellogenic stage to make it fertile. The acquisition of fertile YY-pF laid the foundation for the widespread application of GMT.

Acute physiological responses and muscle recovery in females: a randomised controlled trial of muscle damaging exercise in hypoxia

BackgroundStudies have investigated the effects of training under hypoxia (HYP) after several weeks in a male population. However, there is still a lack of knowledge on the acute hypoxic effects on physiology and muscle recovery in a female population.MethodsThis randomized-controlled trial aimed to investigate the acute effects of muscle damaging exercise, performed in HYP and normoxia (CON), on physiological responses and recovery characteristics in healthy females. Key inclusion criteria were recreationally active female participants between the age of 18 to 35 years without any previous surgeries and injuries, whilst key exclusion criteria were acute pain situations, pregnancy, and medication intake. The females conducted a muscle-damaging protocol, comprising 5 × 20 drop-jumps, in either HYP (FiO2: 12%) or CON (FiO2: 21%). Physiological responses, including capillary oxygenation (SpO2), muscle oxygenation (SmO2), heart rate (HR), core- (Tcore) and skin- (Tskin) temperature were assessed at the end of each exercise set. Recovery characteristics were quantified by taking venous blood samples (serum creatine-kinase [CK], C-reactive protein [CRP] and blood sedimentation rate [BSR]), assessing muscle swelling of the quadriceps femoris muscle, maximum voluntary isometric contraction (MVIC) of the knee extensor muscles, countermovement jump (CMJ) performance and muscle soreness ratings (DOMS) at 24-, 48- and 72-hrs post-exercise.ResultsSpO2 (HYP: 76.7 ± 3.8%, CON: 95.5 ± 1.7%, p < 0.001) and SmO2 (HYP: 60.0 ± 9.3, CON: 73.4 ± 5.8%, p = 0.03) values were lower (p < 0.05) in HYP compared to CON at the end of the exercise-protocol. No physiological differences between HYP and CON were observed for HR, Tcore, and Tskin (all p > 0.05). There were also no differences detected for any recovery variable (CK, CRP, BSR, MVIC, CMJ, and DOMS) during the 72-hrs follow-up period between HYP and CON (all p > 0.05).ConclusionIn conclusion, our results showed that muscle damaging exercise under HYP leads to reduced capillary and muscle oxygenation levels compared to normoxia with no difference in inflammatory response and muscle recovery during 72 h post-exercise.Trial registrationNCT04902924, May 26th 2021.

Abstract 4851: Burden and inequality of early-onset tracheal bronchus, and lung cancer in US, China, and India: A trend and comparison analysis

Abstract Background: For tracheal, bronchus, and lung (TBL) cancer, the leading cause of cancer morbidity and mortality worldwide, guaranteeing quality health care for all populations is a critical component of achieving universal health coverage. Little is known about the burden and inequality of early-onset TBL cancer in countries with different economic development levels. This study aimed to compare the trends of TBL cancer burden among adults in the United States (US), China and India. Methods: Early-onset TBL cancer age was defined as 15-49 years old. We used Joinpoint regression to evaluate the average annual percentage change (AAPC) and its 95% confidence intervals (CIs) to quantify trends in incidence, mortality, and disability-adjusted life years (DALY) based on the Global Burden of Disease 2019 database. We also used the Gini coefficient (GC) to measure the age-standardized DALY rate (ASDR) inequalities across three countries. Results: The incidence, mortality, and DALY rates for early-onset TBL cancer among Americans. population from 1990 to 2019 declined from 7.81/105 to 4.23/105, 5.95/105 to 2.96/105, and 270.54/105 to 133.41/105, respectively. However, among the Chinese and Indian populations, the incidence (China: 5.30/105 to 7.47/105; India: 0.99/105 to 1.38/105), mortality (China: 4.73/105 to 5.82/105; India: 0.90/105 to 1.23/105) and DALY (China: 227.94/105 to 270.26/105; India: 42.89/105 to 58.64/105) rate of early-onset TBL cancer show upward trend from 1990 to 2019. The AAPC in incidence (China: 1.14, 95%CI: 0.87 to 1.40; India: 1.17, 95%CI: 0.85 to 1.49), mortality (China: 0.68, 95%CI: 0.45 to 0.91; India: 1.17, 95%CI: 0.81 to 1.52) and DALY ( China: 0.50, 95%CI: 0.18 to 0.86; India: 1.11, 95%CI: 0.78 to 1.43) rates for early-onset TBL cancer from 1990 to 2019 in both Chinese and Indian populations showed an increasing trend and the increasing trend was higher than the 50-74 age group. While the AAPC in American populations presented a decreasing trend of early-onset TBL cancer (incidence: -1.76, 95%CI: -2.14 to -1.37, mortality: -2.33, 95%CI: -2.56 to -2.10, DALY: -2.35, 95%CI: -2.59 to -2.12). The AAPC in incidence mortality and DALY rates for early-onset TBL cancer from 1990 to 2019 in both the Chinese and Indian female populations showed a higher trend than the male population. The GC value of ASDR for TBL cancer showed a downward trend, from 0.32 in 1990 to 0.24 in 2019; but for early-onset TBL cancer, it showed an upward trend, from 0.28 in 1990 to 0.31 in 2019, across three countries. Conclusions: Compared to the US, early-onset TBL cancer burden in China and India has been rising annually. The inequality in ASDR for early-onset TBL cancer remains high across all three countries. Redistributing attention and resources to help adults in low or middle socioeconomic-level countries, especially the female population, may help hold back the expanding inequality for TBL cancer. Citation Format: Wenxuan Li, Guojin Si, Yacong Zhang, Zhangyan Lyu, Kexin Chen. Burden and inequality of early-onset tracheal bronchus, and lung cancer in US, China, and India: A trend and comparison analysis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4851.

Sexual Dysfunctions among Diabetic and Non-diabetic People Attending in Tribhuvan University Teaching Hospital, Kathmandu Nepal.

Sexual dysfunctions including erectile dysfunction among men, a widespread sexual health issue, pose challenges to sexual satisfaction. This cross-sectional study aimed to assess the prevalence and determinants of sexual dysfunctions in both diabetic and non-diabetic individuals aged 30-70. A study at Tribhuvan University Teaching Hospital in Kathmandu, Nepal, surveyed 350 participants (176 men, 174 women), aged 30-70, with 52.6% having diabetes. The structured interviews and validated questionnaires like IIEF-5 for men and FSFI-6 for women to assess the prevalence and factors associated with erectile dysfunction were used. Statistical tools were employed to measure the associations of different variables with Sexual dysfunctions. Overall, the prevalence of sexual dysfunction was 73.7% (95% CI: 72.4- 73.7) with higher rates in men 83.9% (95% CI: 83.1- 84.7) than women 63.6% (95% CI: 62.0 - 65.2). Individuals with diabetes experienced an 81.5% prevalence of sexual dysfunction (95% CI: 80.6-82.4), whereas non-diabetic individuals exhibited a 65.1% prevalence (95% CI: 63.5-66.7). In the diabetic male population, the prevalence of sexual dysfunction was 97.5% (95% CI: 97.4-97.6), while diabetic females had a prevalence of 68.9% (95% CI: 67.5-70.3). Among non-diabetic men, the percentage of erectile dysfunction was 72% (95% CI: 70.7-73.3), and among non-diabetic women, sexual dysfunction remained 56.2% (95% CI: 54.4-58.0). Among individuals with diabetes, those who used tobacco exhibited a sexual dysfunction prevalence of 93.8% (95% CI: 93.5-94.1), while non-tobacco users had a prevalence of 74.8% (95% CI: 73.6-76.0). In non-diabetic individuals, obesity was associated with a higher prevalence of sexual dysfunctions, reaching 84.6% (95% CI: 83.8-84.6). High blood pressure showed a strong association with sexual dysfunctions in both diabetic (83% with 95% CI: 81.9-83.4) and non-diabetic (70% with 95% CI: 67.7-70.1) groups. Individuals with diabetes for more than five years had a higher rate of sexual dysfunction as 87.8% (95% CI: 86.6-89.0) with 100% in men and 79% in women. However, there was no significant difference in the prevalence of sexual dysfunctions related to obesity and alcohol consumption between diabetics and non-diabetics. The research highlights a noteworthy association of sexual dysfunctions with individuals with diabetes, male sex, tobacco use, and hypertension. The observed high prevalence of sexual dysfunctions in both diabetic and non diabetic people is a public health concern, emphasizing the need for culturally tailored approaches to address the sexual health of the affected individuals.