Makerspace is an important practical platform for "mass entrepreneurship and innovation". Based on the entrepreneurial ecosystem, this paper analyzes the structure, characteristics and operation mechanism of makerspace, and concludes that the system structure of makerspace mainly consists of five interrelated elements, namely maker culture, maker ecosystem, capital ecosystem, resources ecosystem and support platform, and maintains ecological equilibrium through the three major operation mechanisms: resource acquisition and integration mechanism, dynamic competition mechanism and value exchange mechanism. Finally, in response to the current problems in the Makerspaces, development strategies for the makerspace are proposed from four aspects: promoting the application of modern information technology, coordinating planning and layout, strengthening policy implementation, and cultivating and promoting the maker culture.