Major Block Research Articles

A Tomada de decisão em prevenção quaternária

Introdução: O processo de tomada de decisões na prática clínica pode ser analisado sobre diversos aspectos, que confluem em um caminho que tramita entre o conhecimento teórico e a sua aplicação na prática. Nas situações que envolvem bioética, as questões que envolvem as tomadas de decisão são ainda mais complexas, considerando que são momentos que tangenciam a subjetividade dos atores envolvidos. Objetivo: Verificar as tendências de justificativa bioética durante as tomadas de decisão que envolvem situações clínicas de prevenção quaternária entre residentes e preceptores de medicina de família e comunidade. Métodos: Qualitativo, utilizando como instrumento entrevistas projetivas individuais, com vinhetas clínicas fictícias como gatilho das perguntas norteadoras da entrevista. Após a transcrição das mesmas, foi realizado a técnica de análise temática do material. Resultados: Os participantes tiveram uma tendência em justificar suas escolhas de tomada de decisão através de três grandes blocos temáticos, que se relacionam entre si. O primeiro bloco foi a justificativa das tomadas de decisão pelos conhecimentos científicos, o segundo bloco foi baseado nos princípios biomédicos principialistas e o terceiro bloco foi um conteúdo relacionado a relação médico paciente. Conclusões: Pela discussão destes resultados foi possível verificar que existe uma trama entre objetividade clínica e subjetividade pessoal, que não demonstram ser concorrentes, mas sim simbióticas, para alcançar o fim único que é a beneficência do usuário.

Refashioning the EU Visa Policy: A New Turn of the Screw to Cooperation on Readmission and to Discrimination?

Abstract Since the establishment of the Schengen area, border management has been having momentum within the European Union (EU) and, one of its major building blocks is the common policy on visas. This allows to control who can have access to the Schengen area for short stays that do not exceed three months over any six-month period. This article investigates how the EU visa policy has been influenced by the ever-evolving migratory dynamics towards the EU. Focusing on the latest reform of the Common Code on Visa, which has entered into force in 2020, this article argues that the new approach to conditionality in EU migration law has consolidated the intertwining between the EU visa policy and cooperation on readmission. Over the years, the latter has constituted an incentive for the EU in order to offer visa facilitation regimes or specific visa waivers to nationals of third countries. However, this article criticizes the new mechanism, introduced by the recent reform, and providing the European Commission with the mandate to propose specific restrictive measures related to visa processing and visa fee in case of a lack of cooperation especially on readmission. In an attempt to shed light on the legal concerns raised by this mechanism, the paper concludes that it even emphasises the discriminatory effects of the EU visa policy and, more generally, it impacts on the cooperation with third countries within the EU General Approach to Migration and Mobility.

The Interaction between Grains during Columnar-to-Equiaxed Transition in Laser Welding: A Phase-Field Study

A phase-field model was applied to study CET (columnar-to-equiaxed transition) during laser welding of an Al-Cu model alloy. A parametric study was performed to investigate the effects of nucleation undercooling for the equiaxed grains, nucleation density and location of the first nucleation seed ahead of the columnar front on the microstructure of the fusion zone. The numerical results indicated that nucleation undercooling significantly influenced the occurrence and the time of CET. Nucleation density affected the occurrence of CET and the size of equiaxed grains. The dendrite growth behavior was analyzed to reveal the mechanism of the CET. The interactions between different grains were studied. Once the seeds ahead of the columnar dendrites nucleated and grew, the columnar dendrite tip velocity began to fluctuate around a value. It did not decrease until the columnar dendrite got rather close to the equiaxed grains. The undercooling and solute segregation profile evolutions of the columnar dendrite tip with the CET and without the CET had no significant difference before the CET occurred. Mechanical blocking was the major blocking mechanism for the CET. The equiaxed grains formed first were larger than the equiaxed grains formed later due to the decreasing of undercooling. The size of equiaxed grain decreased from fusion line to center line. The numerical results were basically consistent with the experimental results obtained by laser welding of a 2A12 Al-alloy.

Low-Jitter CMOS Digital PLL for the generation of the clock in synchronous serial communication systems

The major building block of linear systems is the Phased Locked-Loop(PLL). Contrasting to analog PLL, the clock period profile of digital PLL (DPLL) is often segmented. Hence the phase and/or frequency tracking of traditional PLL’s may suffer from large jitter when Voltage Controlled Oscillator (VCO) operating point shifts from one clock period segment to another. To address this issue, a Low-Jitter CMOS Digital PLL for the generation of the clock in synchronous serial communication systems is presented in this paper. The design of the PLL will be implemented by considering important CMOS parameters like propagation delay, jitter performance, and power dissipation. Designing of digital PLL includes the design of a phase detector, LPF, Error amplifier, and VCO. The VLSI realization of digital PLL is implemented using the TANNER EDA software tool using a 0.18μm CMOS process.

Cell transplantation to replace retinal ganglion cells faces challenges - the Switchboard Dilemma.

The mammalian retina displays incomplete intrinsic regenerative capacities; therefore, retina degeneration is a major cause of irreversible blindness such as glaucoma, age-related macular degeneration and diabetic retinopathy. These diseases lead to the loss of retinal cells and serious vision loss in the late stage. Stem cell transplantation is a great promising novel treatment for these incurable retinal degenerative diseases and represents an exciting area of regenerative neurotherapy. Several suitable stem cell sources for transplantation including human embryonic stem cells, induced pluripotent stem cells and adult stem cells have been identified as promising target populations. However, the retina is an elegant neuronal complex composed of various types of cells with different functions. The replacement of these different types of cells by transplantation should be addressed separately. So far, retinal pigment epithelium transplantation has achieved the most advanced stage of clinical trials, while transplantation of retinal neurons such as retinal ganglion cells and photoreceptors has been mostly studied in pre-clinical animal models. In this review, we opine on the key problems that need to be addressed before stem cells transplantation, especially for replacing injured retinal ganglion cells, may be used practically for treatment. A key problem we have called the Switchboard Dilemma is a major block to have functional retinal ganglion cell replacement. We use the public switchboard telephone network as an example to illustrate different difficulties for replacing damaged components in the retina that allow for visual signaling. Retinal ganglion cell transplantation is confronted by significant hurdles, because retinal ganglion cells receive signals from different interneurons, integrate and send signals to the correct targets of the visual system, which functions similar to the switchboard in a telephone network – therefore the Switchboard Dilemma.

Tracing Metabolic Fate of Mitochondrial Glycine Cleavage System Derived Formate In Vitro and In Vivo.

Folate-mediated one-carbon (1C) metabolism is a major target of many therapies in human diseases. Studies have focused on the metabolism of serine 3-carbon as it serves as a major source for 1C units. The serine 3-carbon enters the mitochondria transferred by folate cofactors and eventually converted to formate and serves as a major building block for cytosolic 1C metabolism. Abnormal glycine metabolism has been reported in many human pathological conditions. The mitochondrial glycine cleavage system (GCS) catalyzes glycine degradation to CO2 and ammonium, while tetrahydrofolate (THF) is converted into 5,10-methylene-THF. GCS accounts for a substantial proportion of whole-body glycine flux in humans, yet the particular metabolic route of glycine 2-carbon recycled from GCS during mitochondria glycine decarboxylation in hepatic or bone marrow 1C metabolism is not fully investigated, due to the limited accessibility of human tissues. Labeled glycine at 2-carbon was given to humans and primary cells in previous studies for investigating its incorporations into purines, its interconversion with serine, or the CO2 production in the mitochondria. Less is known on the metabolic fate of the glycine 2-carbon recycled from the GCS; hence, a model system tracing its metabolic fate would help in this regard. We took the direct approach of isotopic labeling to further explore the in vitro and in vivo metabolic fate of the 2-carbon from [2-13C]glycine and [2-13C]serine. As the 2-carbon of glycine and serine is decarboxylated and catabolized via the GCS, the original 13C-labeled 2-carbon is transferred to THF and yield methyleneTHF in the mitochondria. In human hepatoma cell-lines, 2-carbon from glycine was found to be incorporated into deoxythymidine (dTMP, dT + 1), M + 3 species of purines (deoxyadenine, dA and deoxyguanine, dG), and methionine (Met + 1). In healthy mice, incorporation of GCS-derived formate from glycine 2-carbon was found in serine (Ser + 2 via cytosolic serine hydroxy methyl transferase), methionine, dTMP, and methylcytosine (mC + 1) in bone marrow DNA. In these experiments, labeled glycine 2-carbon directly incorporates into Ser + 1, A + 2, and G + 2 (at C2 and C8 of purine) in the cytosol. It is noteworthy that since the serine 3-carbon is unlabeled in these experiments, the isotopic enrichments in dT + 1, Ser + 2, dA + 3, dG + 3, and Met + 1 solely come from the 2-carbon of glycine/serine recycled from GCS, re-enters the cytosolic 1C metabolism as formate, and then being used for cytosolic syntheses of serine, dTMP, purine (M + 3) and methionine. Taken together, we established model systems and successfully traced the metabolic fate of mitochondrial GCS-derived formate from glycine 2-carbon in vitro and in vivo. Nutritional supply significantly alters formate generation from GCS. More GCS-derived formate was used in hepatic serine and methionine syntheses, whereas more GCS-derived formate was used in dTMP synthesis in the bone marrow, indicating that the utilization and partitioning of GCS-derived 1C unit are tissue-specific. These approaches enable better understanding concerning the utilization of 1C moiety generated from mitochondrial GCS that can help to further elucidate the role of GCS in human disease development and progression in future applications. More studies on GCS using these approaches are underway.

Evidence from APOGEE for the presence of a major building block of the halo buried in the inner Galaxy

ABSTRACT We report evidence from APOGEE for the presence of a new metal-poor stellar structure located within ∼4 kpc of the Galactic Centre. Characterized by a chemical composition resembling those of low-mass satellites of the Milky Way, this new inner Galaxy structure (IGS) seems to be chemically and dynamically detached from more metal-rich populations in the inner Galaxy. We conjecture that this structure is associated with an accretion event that likely occurred in the early life of the Milky Way. Comparing the mean elemental abundances of this structure with predictions from cosmological numerical simulations, we estimate that the progenitor system had a stellar mass of ∼5 × 108 M⊙, or approximately twice the mass of the recently discovered Gaia-Enceladus/Sausage system. We find that the accreted:in situ ratio within our metal-poor ([Fe/H] < –0.8) bulge sample is somewhere between 1:3 and 1:2, confirming predictions of cosmological numerical simulations by various groups.

And… Cut!

AbstractAlong with wind energy, photovoltaics is one of the major building blocks of the energy transition. But it is also regarded as one of the greatest social challenges of our time. Twenty years ago, the production of crystalline silicon solar cells and thin‐film modules was still in its infancy. Much has changed since then as manufacturing in small batches has long since given way to mass production.

Delivery of Pebbles from the Protoplanetary Disk into Circumplanetary Disks

Abstract Small bodies likely existed in the late stage of planet formation either as remnants of the planetesimal formation stage or as fragments of larger planetesimals. Recent studies suggest that they may have played an important role in the formation of regular satellites of giant planets, but their delivery process into the circumplanetary disk has been poorly understood. Using orbital integration that incorporates the gas flow around the planet obtained by hydrodynamic simulation, we examine delivery of small bodies in the protoplanetary disk into circumplanetary disks. We find that large bodies can be captured when they experience strong gas drag near the midplane of the circumplanetary disk, while particles with Stokes number near unity tend to settle toward the midplane of the protoplanetary disk and can be captured near the outer edge of the circumplanetary disk. On the other hand, small particles coupled to the gas can be delivered into the circumplanetary disk with the vertically accreting gas and are captured near the surface of the circumplanetary disk over a wide radial region, if they are sufficiently stirred off the midplane of the protoplanetary disk. However, if the turbulence in the protoplanetary disk is not sufficiently strong, delivery of small particles by such a mechanism would not be efficient. Also, gas depletion in the vicinity of the planet’s orbit reduces the efficiency of the delivery. In these cases, larger bodies directly captured by gas drag from the circumplanetary disk would be the major building blocks of regular satellites.

Public electric vehicle charger access disparities across race and income in California

Widespread electric vehicle (EV) adoption is crucial for achieving California's climate goals. The inclusion of marginalized populations in this process is important and will require that they have access to charging infrastructure. Public EV charging stations may help reduce the EV adoption barriers affecting these populations. This study combines public charging station location data with American Community Survey data at the census block group level in California, finding that public charger access is lower in block groups with below-median household incomes and in those with a Black and Hispanic majority populations. These public charger access disparities are more pronounced in areas with a higher proportion of multi-unit housing, where they are critical for EV operation due to a lower likelihood of residential charger access. Controlling for distance to the nearest highway or freeway, multi-unit housing unit rate, and median household income, we find that Black and Hispanic majority block groups are the only race and ethnicity group that is significantly less likely to have access to any public charger in their block groups compared to the rest of the state. The odds of having public charger access for the group is 0.7-times that of the no majority reference group. The access gap is even larger for the publicly-funded charging stations where Black and Hispanic majority block groups are approximately half as likely as the no-majority reference group to have access. Hence directing a larger portion of the funding to underserved communities and further government involvement in filling the public charger access gap can be crucial in achieving widespread and equitable EV adoption.

Genetic regulation of gene expression of MIF family members in lung tissue

Macrophage migration inhibitory factor (MIF) is a cytokine found to be associated with chronic obstructive pulmonary disease (COPD). However, there is no consensus on how MIF levels differ in COPD compared to control conditions and there are no reports on MIF expression in lung tissue. Here we studied gene expression of members of the MIF family MIF, D-Dopachrome Tautomerase (DDT) and DDT-like (DDTL) in a lung tissue dataset with 1087 subjects and identified single nucleotide polymorphisms (SNPs) regulating their gene expression. We found higher MIF and DDT expression in COPD patients compared to non-COPD subjects and found 71 SNPs significantly influencing gene expression of MIF and DDTL. Furthermore, the platform used to measure MIF (microarray or RNAseq) was found to influence the splice variants detected and subsequently the direction of the SNP effects on MIF expression. Among the SNPs found to regulate MIF expression, the major LD block identified was linked to rs5844572, a SNP previously found to be associated with lower diffusion capacity in COPD. This suggests that MIF may be contributing to the pathogenesis of COPD, as SNPs that influence MIF expression are also associated with symptoms of COPD. Our study shows that MIF levels are affected not only by disease but also by genetic diversity (i.e. SNPs). Since none of our significant eSNPs for MIF or DDTL have been described in GWAS for COPD or lung function, MIF expression in COPD patients is more likely a consequence of disease-related factors rather than a cause of the disease.

A Review of Microelectronic Systems and Circuit Techniques for Electrical Neural Recording Aimed at Closed-Loop Epilepsy Control.

Closed-loop implantable electronics offer a new trend in therapeutic systems aimed at controlling some neurological diseases such as epilepsy. Seizures are detected and electrical stimulation applied to the brain or groups of nerves. To this aim, the signal recording chain must be very carefully designed so as to operate in low-power and low-latency, while enhancing the probability of correct event detection. This paper reviews the electrical characteristics of the target brain signals pertaining to epilepsy detection. Commercial systems are presented and discussed. Finally, the major blocks of the signal acquisition chain are presented with a focus on the circuit architecture and a careful attention to solutions to issues related to data acquisition from multi-channel arrays of cortical sensors.

High-K granites between the Archean Gavião and Jequié blocks, São Francisco Craton, Brazil: Implications for cratonization and amalgamation of the Rhyacian Atlantica continent

Precambrian terrane boundaries are sites of diverse rock associations and structural complexity. Motions along the boundary zones are commonly related to the construction of ancient large continental masses. This seems to be the case in the São Francisco Craton, Brazil, where three major blocks (Gavião, Jequié and Serrinha) collided in the Paleoproterozoic to form the northern part of the craton. This manuscript presents zircon U-Pb ages and whole-rock geochemistry for rock associations on the western border of the Jequié Block, which is defined by the north-trending left-lateral 600-km long Contendas-Jacobina fault zone. The studied rocks are granulite to amphibolite facies Neoarchean orthopyroxene-hornblende granite gneisses of the Jequié block, which also occur tectonically imbricated within Paleoproterozoic metavolcanosedimentary rocks of the Contendas-Mirante belt. The rocks are 2.65-2.63 Ga high-K ferroan, metaluminous to slightly peraluminous syeno-monzogranite in composition, with negative ƐNd(2650) and Nd model ages of 3.05-2.88 Ga. They are depleted in Sr and Ti, enriched in Th, Ba and Pb enrichment, and have marked negative Eu/Eu*. This geochemical signature coupled with the Nd-isotope data indicate that the parental granitic magma was generated by crustal reworking of felsic Mesoarchean crust distinct from the western-sitting >3.3 Ga Gavião Block. The investigated granitic gneisses are similar to other potassic granitoids that occur in the São Francisco Craton, which mark the thermal stabilization of the Archean lithosphere. The westward thrusting of the Neoarchean hornblende granitic gneiss and associated high-Mg tremolitite (meta-basalts) onto Paleoproterozoic volcano-sedimentary sequences establishes field evidence of Paleoproterozoic nappe stacking before lateral displacement during block collision. The crustal amalgamation is related to the assembly of the São Francisco-Congo Craton, a major landmass established before the Columbia supercontinent.

A Double Difference Tomography Study of the Washington Forearc: Does Siletzia Control Crustal Seismicity?

AbstractWe present new seismic tomography of the Washington forearc using a suite of manually picked regional earthquake phase arrivals. The recovery of similarly sampled P and S velocity permits the robust calculation of Poisson's ratio throughout the region. The seismological signature of Siletzia, an accreted oceanic plateau that crops out in Washington as the Crescent Formation, is evident in our models as a continuous high Poisson's ratio body that coincides with subsurface structures estimated from potential field maps. Relocated earthquakes preferentially locate in low Poisson's ratio regions in the forearc crust and, in particular, in a diffuse layer located at 15–25 km depth in the crust beneath relatively aseismic Siletzia. Our imaging of the Puget Sound is consistent with previous interpretations of the architecture of major faults and blocks, as evident by distinct Poisson's ratio signatures that distinguish sedimentary basins from mafic rocks of the Crescent Formation. We speculate that seismicity below the Puget Sound is promoted by slab‐derived fluids that are localized beneath Siletzia as a result of intrinsic low vertical permeability.

Hyperventilation-Apnoea Syndrome in a 79-year Old Woman with Congestive Heart Failure

We report the case of a 79-year woman admitted for daily episodes of sudden breath arrest abruptly occurred one month prior to hospital presentation. These episodes are preceded by hyperventilation due to physical activity, especially when working in bending forward position. She was diagnosed and treated for high blood pressure, coronary heart disease, major left bundle branch block, congestive heart failure, obesity, hypercholesterolaemia and hypothyroidism. Spirometry was normal, chest x-ray showed features of basal bilateral pulmonary fibrosis, overnight respiratory polygraphy revealed moderate obstructive sleep apnoea, while cerebral magnetic resonance imaging showed a right ventrolateral compression of the medulla oblongata – spinal cord junction by a dominant, sinuous right vertebral artery, and loss of foramen magnum cerebrospinal fluid due to low lying cerebellar tonsils tips, while cisterna magna is still visible. The rare breathing pattern of our patient is reported in the literature as hyperventilation-apnoea syndrome and we discuss the potential mechanisms underlying this condition.

Grad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichiacoli.

Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here, we performed Grad-seq to establish an RNA/protein complexome, reconstructing sedimentation profiles in a glycerol gradient for ∼85% of all E. coli transcripts and ∼49% of the proteins. These include the majority of small noncoding RNAs (sRNAs) detectable in this bacterium as well as the general sRNA-binding proteins, CsrA, Hfq and ProQ. In presenting use cases for utilization of these RNA and protein maps, we show that a stable association of RyeG with 30S ribosomes gives this seemingly noncoding RNA of prophage origin away as an mRNA of a toxic small protein. Similarly, we show that the broadly conserved uncharacterized protein YggL is a 50S subunit factor in assembled 70S ribosomes. Overall, this study crucially extends our knowledge about the cellular interactome of the primary model bacterium E. coli through providing global RNA/protein complexome information and should facilitate functional discovery in this and related species.

Brownian motion in trapping enclosures: steep potential wells, bistable wells and false bistability of induced Feynman–Kac (well) potentials

We investigate signatures of convergence for a sequence of diffusion processes on a line, in conservative force fields stemming from superharmonic potentials U(x) ∼ x m , m = 2n ⩾ 2. This is paralleled by a transformation of each mth diffusion generator L = DΔ + b(x)∇, and likewise the related Fokker–Planck operator L* = DΔ − ∇[b(x) ⋅], into the affiliated Schrödinger one . Upon a proper adjustment of operator domains, the dynamics is set by semigroups exp(tL), exp(tL*) and exp(−tĤ), with t ⩾ 0. The Feynman–Kac integral kernel of exp(−tĤ) is the major building block of the relaxation process transition probability density, from which L and L* actually follow. The spectral ‘closeness’ of the pertinent Ĥ and the Neumann Laplacian in the interval is analyzed for m even and large. As a byproduct of the discussion, we give a detailed description of an analogous affinity, in terms of the m-family of operators Ĥ with a priori chosen , when Ĥ becomes spectrally ‘close’ to the Dirichlet Laplacian for large m. For completness, a somewhat puzzling issue of the absence of negative eigenvalues for Ĥ with a bistable-looking potential has been addressed.

Spiral Hierarchical Superstructures from Twisted Ribbons of Self-Assembled Chiral Block Copolymers

Spiral hierarchical superstructures were found in the self-assembly of chiral block copolymers (BCPs*) composed of a chiral poly(l-lactide) (PLLA) and an achiral polystyrene (PS) as major and minor blocks, respectively. The PLLA helical chain with semiflexible rod-like character as compared to the random coil of PS results in self-assembly with a conformational asymmetry effect overwhelming the compositional one. Consequently, instead of the forming PS cylinder microdomains in the PLLA matrix, a smectic liquid-crystal-like bilayer sandwiched with PLLA and PS microdomains will be formed. Owing to twisting and bending due to the chiral cholesteric liquid-crystal-like force field combined with steric hindrance at the chiral interface, the forming bilayers (twisted ribbon) will develop into either a concentric lamellar texture from scrolling or roll-cake textures from spiraling. This study might bring a concept for the formation of spiral hierarchical superstructures from self-assembled bilayers for device application.

β-Lactoglobulin Adsorption Layers at the Water/Air Surface: 4. Impact on the Stability of Foam Films and Foams

The complexity and high sensitivity of proteins to environmental factors give rise to a multitude of variables, which affect the stabilization mechanisms in protein foams. Interfacial and foaming properties of proteins have been widely studied, but the reported unique effect of pH, which can be of great interest to applications, has been investigated to a lesser extent. In this paper, we focus on the impact of pH on the stability of black foam films and corresponding foams obtained from solutions of a model globular protein—the whey β-lactoglobulin (BLG). Foam stability was analyzed utilizing three characteristic parameters (deviation time, transition time and half-lifetime) for monitoring the foam decay, while foam film stability was measured in terms of the critical disjoining pressure of film rupture. We attempt to explain correlations between the macroscopic properties of a foam system and those of its major building blocks (foam films and interfaces), and thus, to identify structure-property relationships in foam. Good correlations were found between the stabilities of black foam films and foams, while relations to the properties of adsorption layers appeared to be intricate. That is because pH-dependent interfacial properties of proteins usually exhibit an extremum around the isoelectric point (pI), but the stability of BLG foam films increases with increasing pH (3–7), which is well reflected in the foam stability. We discuss the possible reasons behind these intriguingly different behaviors on the basis of pH-induced changes in the molecular properties of BLG, which seem to be determining the mechanism of film rupture at the critical disjoining pressure.

A method for quick capital cost estimation of biorefineries beyond the state of the art

AbstractThis paper presents the ‘process blocks build‐up estimating’ method for the capital cost estimation of biorefineries at Technology Readiness Levels 2 to 3 with a −50% to +100% accuracy, corresponding to a Class 5 estimate. Through systematic cost modeling, the method introduces a modular approach to cost estimation by breaking down a biorefinery into its major process blocks and using separate cost power functions. Suitable cost parameters (exponents and reference capital costs) for 11 biochemical, chemical and thermochemical biorefinery types are developed, as well as reference block efficiencies for estimating the costs of biorefineries within and beyond the state of the art. A framework for uncertainty modeling is also proposed to decide on contingencies for a biorefinery investment. The cost models are addressed to the biorefinery community, from experimental chemists to process engineers and decision makers, to assist them in selecting optimal pathways and budgeting new projects. The modular estimation approach is illustrated with a simple example on a biodiesel plant and a first‐of‐a‐kind polyhydroxybuturate (PHB) plant. © 2020 The Authors. Biofuels, Bioproducts and Biorefining published by Society of Industrial Chemistry and John Wiley & Sons Ltd