The article deals with the current issue of development of the transport and logistics system and transport and logical clusters on the territory of the Russian Federation on the basis of network interaction. The subject of the study is the transport and logistics clusters of Russian regions, their importance in the development of the transport and logistics complex and economy of Russian regions. Scientific and theoretical concepts on the research problem and their hierarchy in the transport and logistics complex of the region are considered. The economic essence of the transport and logistics cluster and its structure elements are described, and the economic characteristics of various groups of enterprises depending on the degree of participation in the main business process are presented. A comparative assessment of the state and functioning of transport and logistics clusters on the territory of Russia with the European network of transport and logistics clusters formed during the strengthening of integration of European economies into a single space is carried out and proposals for their development based on network interaction are made. The main research methods are formal logical, system and situational approaches, and statistical analysis. The need to support the formation of transport and logistics clusters through state programs and strategies, forms of public-private partnership in economy, activation of integration ties and integrated development based on basic enterprises is identified. The indicators of functioning of transport and logical clusters on the example of Belgorod region, Primorye Krai, including the level of development of the regional infrastructure, the degree of involvement of the economic power of the region, regions position on major domestic and global transport corridors are analyzed and studied. Based on the results of the work, proposals for the development of network interaction in the transport and logistics complex of the regions were formulated, prospects and directions for improving the economic efficiency of transport enterprises were determined, and the percentage of losses in the industry was reduced. The application of the results is useful in the development of regional socio-economic and transport development programs.