Major Food Items Research Articles

Lipidomic perturbations of normal-weight adiposity phenotypes and their mediations on diet–adiposity associations

Background & aimsNormal-weight obesity (NWO) and normal-weight central obesity (NWCO) have been linked to higher cardiometabolic risks, but their etiological bases and attributable dietary factors remain unclear. In this study we therefore aimed to identify lipidomic signatures and dietary factors related to NWO and NWCO and to explore the mediation associations of lipids in diet–adiposity associations. MethodsUsing a high-coverage targeted lipidomic approach, we quantified 1245 serum lipids in participants with NWO (n = 150), NWCO (n = 150), or propensity-score-matched normal-weight controls (n = 150) based on the Regional Ethnic Cohort Study in Northwest China. Consumption frequency of 28 major food items was recorded using a food frequency questionnaire. ResultsProfound lipidomic perturbations of NWCO relative to NWO were observed, and 249 (dominantly glycerolipids) as well as 48 (dominantly glycerophospholipids) lipids were exclusively associated with NWCO or NWO. Based on strong lipidomic signatures identified by a LASSO model, phospholipid biosynthesis was the top enriched pathway of NWCO, and sphingolipid metabolism was the top pathway of NWO. Remarkably, sphingolipids were positively associated with NWO and NWCO, but lyso-phosphatidylcholines were negatively associated with them. Rice, fruit juice, and carbonated drink intakes were positively associated with the risk of NWCO. Both global and individual lipidomic signatures, including SE(28:1_22:6) and HexCer(d18:1/20:1), mediated these diet-NWCO associations (mediation proportion: 15.92%–26.10%). ConclusionsDifferential lipidomic signatures were identified for overall and abdominal adiposity accumulation in normal-weight individuals, underlining their core mediation roles in dietary contributions to adiposity deposition.

Development of Omani-branded food composition database for an electronic dietary assessment tool

Background: The food composition database provides a comprehensive information on the various nutrients present in the foods. At present, Oman lacks a food composition database (FCDB) of locally produced branded foods, which necessitates the development of such a database. Objectives: The aim of this study is to develop an FCDB for Omani-branded foods available for local consumption for an electronic dietary assessment tool. Methods: Back-of-pack (BOP) nutritional information of these branded foods available in our markets was gathered from manufacturer data. Food mapping was used to match the branded foods according to the BOP macronutrient data and food description to the appropriate generic food item from United States Department of Agriculture (USDA) or Canadian FCDBs. Results: The developed database is composed of 571 food items with associated 60 nutrients. The majority of food items (91%) were mapped to a single generic food item. The rest (9%) was mapped to multiple generic food items. Overall, 96% of food items were mapped to a single generic food, which was matched based on BOP macronutrients and item descriptions. However, the minority (4%) were mapped based on item description alone as either the BOP nutrients were implausible. Moreover, 91% of food items were individually mapped to within 10% agreement with the generic food item for energy. The fish and fish group has the largest mean of absolute percentage difference in energy between BOP and generic items (16%). Conclusion: This currently developed database would critically help to accurately assess the dietary intake of the Omani population once incorporated into an electronic dietary assessment tool, and it can be updated in the future on a regular basis.

Assessment of polychlorinated naphthalenes in Korean foods: Levels, profiles, and dietary intake

Concerns about dioxin-like compounds have increased; however, the monitoring of polychlorinated naphthalenes (PCNs) in food and the assessment of dietary intake remain limited. In this study, various foods were collected from Korean markets and analyzed for PCNs. Fishery products exhibited the highest mean concentration (48.0 pg/g ww) and toxic equivalent (TEQ) (0.0185 pg-TEQ/g ww). Agricultural products were the largest contributors (35.7%) to the total dietary intake of PCNTEQ, followed by livestock products (33.6%), fishery products (20.2%), and processed foods (10.5%). The mean intake of PCNTEQ for the Korean population was 0.901 pg-TEQ/day for males and 0.601 pg-TEQ/day for females. Generally, males and younger groups had higher daily intakes of PCNTEQ, but they did not exceed the tolerable weekly intakes. Nonetheless, it is important to manage potential health risks associated with PCNs and other dioxin-like compounds by identifying major food items contributing to PCN exposure and considering age and gender differences.

Effect of Heat Moisture Treatment, Annealing, and Gelatinization‐Retrogradation Modifications on Physico‐Chemical, Functional, and Structural Properties of Starch from Elephant Foot Yam (Amorphophallus paeoniifolius)

AbstractRoots and tuberous plants are vital food crops used as food supplements in many parts of the tropics. Being a major food item, starch is used in food products as thickeners, adsorbent, film forming agent. Starch, naturally, possess certain limitations & starch modifications can overcome the limitations, thereby increasing its versatility and meeting the needs of consumer. Current study focuses on comparative analysis between modified Elephant Foot Yam (Amorphophallus paeoniifolius) starch using physical methods (heat treatment method, annealing and gelatinization‐retrogradation) and to study the effect of modification on physico‐chemical, functional, structural and thermal properties. Results reveal that all physically modified starches positively altered the functional and physiological characteristics of native yam starch. However, maximum restriction in swelling power (2.54 %), solubility (2.47 %), moisture content (1.96 %), water absorption capacity (1.41 g/mL) is observed in heat moisture treated (HMT) starch. These HMT modified starches can be used in the manufacturing of baked and dairy products having reduced glycemic index, can be used as a packaging material for coating of fruits and vegetables. Henceforth, suitable and relevant application of modified starches of desired physical and functional features may be produced for industrial utilisation, or as additives fulfilling specific purposes in foods.

Food Insecurity Transition Among Agricultural Households In Nigeria

ABSTRACT: Hunger continues to be on the increase and food crises remain a developmental challenge. The incidence of food insecurity in the country has heightened in recent years due to unprecedented natural hazards, economic shocks and climate change, which have eroded livelihoods and worsened the country’s performance in food security-related indices. We therefore used the panel data from Nigeria to examine the food insecurity transition among agricultural households. The data covered four rounds. The food insecurity status of agricultural households was estimated using the Foster-Greer-Thorbecke measure. We explained the interaction among the states of food security of agricultural households across different periods using the food insecurity transition matrix. The determinants of food insecurity were explored using the dynamic probit model. The model addressed unobserved heterogeneity, state dependency, and serial correlation arising from the data and model. Findings revealed that the mean food expenditure increased across the period. Similarly, mean per capita food expenditure also increased across periods except for the 2012/2013 period where it dropped slightly. We observed a rising incidence of food insecurity across the period from 44.1% in 2010/2011 to 53.4% in 2018/2019 representing a 21.1% increase in food insecurity incidence. Widening inequality exists even among the food insecure. Our findings revealed that 87.6% of households are transitorily food insecure, 5.5% are chronically food insecure and 7% are food secured. The significant factors driving food insecurity across the period were the area of land under cultivation, the fall in the price of outputs, and the increase in the price of major food items. Coping strategies employed were eating less preferred foods, restriction in diversity of foods eaten, limiting food portions, and minimizing the frequency of meal consumption. It is therefore important that the government encourage the adoption of yield-enhancing technologies to ensure household food security. Rising prices of major food items and fall in output price gives a cause for concern over the macroeconomic environment in the country, which fuels rising food prices. Policymakers should exercise caution when implementing exchange rate policies that may affect food and output prices, given the impact they have on the livelihood and household security of smallholders.

Characterizing nutrient patterns of food items in adolescent diet using data from a novel citizen science project and the US National Health and Nutrition Examination Survey (NHANES).

A healthy diet is essential for promoting good health during adolescence and mitigating disease risks in adulthood. This underscores the need for improved nutrition education and increased access to healthier food choices. However, the accuracy of dietary data poses a significant challenge in nutritional research. We utilized and analyzed a novel dietary record dataset collected through a high school citizen science project to address this issue. We focused on nutrients rather than food groups to characterize adolescent dietary patterns. The same analyses were performed on the 2019-2021 National Health and Nutrition Examination Survey data for comparison. Based on the U.S. Food and Drug Administration's recommended daily value (DV) for nutrients, the majority of food items in our citizen science dataset are low (i.e., <5% DV) in lipids, fiber, potassium, calcium, iron, sugar, and cholesterol. Only a minority of items are high (i.e., >20% DV) in macro and micronutrients. The clustering analysis identified nine food clusters with distinct nutrient profiles that vary significantly in size. The analyses on the NHANES data yielded similar findings, but with higher proportions of foods high in energy, lipids, carbohydrates, sugar, iron, and sodium compared with those of the citizen science dataset. This study demonstrates the potential of citizen science projects in gathering valuable dietary data and understanding adolescent nutrient intake. Identifying critical nutrient gaps can guide targeted nutrition education and the provision of accessible healthier food options, leading to positive health outcomes during adolescence and beyond.

Food habits of the Red Fox Vulpes vulpes (Mammalia: Carnivora: Canidae) in Dachigam National Park of the Kashmir Himalaya, India

Food habits of the Red Fox Vulpes vulpes were studied in Dachigam National Park of the Kashmir Himalaya from December 2017 to November 2018 by scat analysis. A total of 246 scats were collected across seasons from different habitat types of the park. The diet of the Red Fox was characterized by a wider prey spectrum with small rodents, plants, and Himalayan Grey Langur Semnopithecus ajax as the major food items. The dietary behaviour showed slight seasonal variation with more invertebrates and plant material (fruits, berries, &amp; grasses) during spring and summer. The food niche of the canid was wider in the warm season than in the cold season. Diet analysis revealed substantial use of anthropogenic food sources (human refuse and livestock carrion) by the Red Fox. The frequency of occurrence of human refuse and livestock carrion in the scats of the canid species varied seasonally. The results show that the Red Fox is an opportunistic feeder, and capable of adapting to a variety of dietary items.

Food Purchasing Behavior in the Jakarta Metropolitan Area

This study investigates food purchasing behaviors in the Jakarta Metropolitan area of Indonesia. A quantitative study was carried out using a web questionnaire survey. Fifteen major food items purchased through E-commerce were selected. Participants were categorized to explore food purchasing behavior. Four main results were identified: (1) food purchases through E-commerce are likely to continue to grow; (2) highly educated consumers tend to purchase food items through E-commerce; (3) food purchase intention through E-commerce is not necessarily related to household expenditures; and (4) in the case of Muslim participants, religion affects their purchasing behavior of several food items.

Plankton Composition and Abundance in Semi Intensive Aquaculture Pond and their Preference by Nile tilapia

Plankton is major food item for Nile tilapia and their availability is one of the most important factors affecting its growth performance in an extensive and semi-intensive pond-based culture systems. Thus, this study aimed to examine plankton composition, abundance, and feeding preference by Nile tilapia reared under a semi-intensive pond-based production system. The experiment was carried out using ponds (10mx15mx1.7m size) fertilized with chicken manure at the rate of 100g/m2/week. Nile tilapia with 7.8g average body weight was stocked at a density of 2 fish/m2. Examination of plankton from both pond water and stomach samples of fish was done using qualitative assessment methods. The results showed that a total of 20 phytoplankton groups were identified following standard identification key. These include four types of Blue-green algae (Cyanophyceae), seven types of Green algae (Chlorophyceae), six Diatoms (Bacillariophyceae), one type of Dinophyceae, and two types of Euglenophyceae. Among the phytoplankton groups, Chlorophyceae was the dominant group with 56% followed by Bacillariophyceae (23%), Cyanophyceae (17%), and Euglenophyceae (3%) while the least was Dinophyceae (1%). The most frequently observed algal genus was Scenedesmus. In addition, three Zooplankton groups namely Copepods (43%), Rotifers (31%) and Cladocerans (26%) were also identified. The result indicated that, the most preferred planktonic groups used by Nile tilapia was green algae, particularly Chlorophyceae followed by Copepoda and Rotifers. The level of chlorophyll `a` was 62.3±2.0 μg/l concentration. The different water quality parameters together with the availability of different plankton were optimal for the growth of Nile tilapia which resulted in a 0.45 specific growth rate. In conclusion, Nile tilapia exhibit preference for green algae, blue-green algae from phytoplankton, and Copepods from zooplankton groups than other groups of plankton.

Socioeconomic determinants of nutritional behaviors of households in Fars Province, Iran, 2018.

IntroductionHouseholds' dietary habits are affected by their environment and socioeconomic status (SES). This study aims to investigate eating behaviors and determine the factors affecting nutritional status in households in Fars Province in 2018.MethodIn this cross-sectional study, urban and rural households were selected using the multistage sampling method. A questionnaire was employed to interview the mother or householder to record the demographic, SES, and dietary habits of the family for major food items commonly used. A logistic regression model was used to analyze the data. The p-value less than 0.05 was considered significant.ResultsIn total, 6,429 households participated in the study. The majority of households use traditional flatbread, low-fat milk, and liquid/cooking oil. Frying was the most prevalent method of cooking. Parents' level of education and SES were associated with type of consumed bread, milk and dairy, methods of food preparation, adding salt at the table, eating out, and fast-food usage. Parents' higher level of education was significantly associated with salt storage in optimal conditions and not using salt before tasting the meal.ConclusionMost of the households had healthy practices, especially in consumption of certain oils and methods of preparing their food as well as keeping salt in an optimal condition and using iodized salt. The most important unhealthy nutritional behavior was high consumption of fast food and outdoor food, especially in urban regions. Unhealthy dietary habits were more prevalent in households with low household and regional SES. Both households and regions with higher SES had better dietary habits.

Oral and Buccophryngeal Morphology of the Tadpoles of Polypedates maculatus with Notes on their Diet pattern

Oral morphological features and the diet of the tadpoles of Polypedates maculatus were described in this study. The tadpoles were collected from various water bodies of the state of Odisha over three consecutive breeding seasons (2018–2020). Oral and internal buccal characters were analysed independently for Gosner developmental stages 25 to 46. The upper labium has four labial tooth rows with a medial gap in the three rows proximal to the mouth, whereas the lower labium has three rows with a medial gap in the row proximal to the mouth. The order of length of denticle rows is A2>P1>P2>A1>P3>A3>A4 with a dental formula of A4(3)/P3(1). The food spectrum of the tadpoles included mostly phytoplanktons (represented by six classes and 51 genera) followed by zooplankton (two classes and four genera). Three Bacillariophyceae genera (Navicula, Pinnularia, and Synedra) contributed most of the gut contents. Scenedesmus was the major food items in Chlorophyceae family and had the highest numeric frequency (7.63%) among all the food items. Other prevalent food items included Zygnematophyceae, Cyanophyceae, Euglenophyceae, Rotifera, Ulvophyceae and Copepoda.

How Does Income Heterogeneity Affect Future Perspectives on Food Consumption? Empirical Evidence from Urban China.

China is undergoing a rapid dietary transition as well as a changing income distribution. In this paper, we examine the impacts of income heterogeneity on the prediction of food consumption using a dataset that covered 22,210 urban households in China’s 6 provinces. The two-stage Exact Affine Stone Index Implicit Marshallian Demand System (EASI demand system) model, which deals with the problem of censoring and endogeneity, is applied to estimate demand elasticity across income strata. Additionally, a dynamic simulation method considering income heterogeneity is conducted to predict future food consumption trends. The results reveal that income elasticity follows a decreasing trend with income growth. Furthermore, the results show that the consumption of major food items in the 15th period will increase by 7.9% to 42.0% over the base period. The growth potential of low-income groups is significantly higher than that of middle- and high-income groups. However, the prediction results may be overestimated if the differences in consumer behavior across income groups and the dynamic simulation procedure are not taken into account. Our study indicates that the consumption features of different income groups need to be included in food consumption forecasts. Moreover, the government should formulate food policies for different income groups to promote a sustainable food system transformation.

Dietary variability of western gorillas (Gorilla gorilla gorilla).

Spatial and temporal variability in the availability of food resources will lead to variation in a species’ diet, which can then influence patterns of space use, sociality, and life history characteristics. Despite such potential impacts, little information is available about dietary variability for some species with large geographical ranges. Here we quantify the diet and nutritional content of plants consumed by western lowland gorillas (Gorilla gorilla gorilla) in Loango National Park, Gabon over a 2.6 year period and make comparisons with two study sites located 800 km away. The major foods consumed by the Loango gorillas differed greatly from the other two study sites, but gorillas at all three locations spent a similar proportion of feeding time consuming herbaceous vegetation and tree leaves (~ 50%) and fruit (35%). The Loango gorillas spent approximately 10% of feeding time eating nuts, which were not consumed at the other two study sites. Gorillas at those sites spent about 5% of feeding time eating insects, which were not consumed by Loango gorillas. Even though the species composition of the diet differed among the three sites, the nutritional composition of the major food items differed very little, suggesting that western gorillas consume foods of similar nutritional values to meet their dietary needs. This study shows the flexibility of diet of a species with a wide geographic distribution, which has implications for understanding variation in life history characteristics and can be useful for conservation management plans.

Consumption Pattern and Elasticities of Demand of Major Food Items among Farm Households in Andhra Pradesh

We examined food consumption pattern of farm households in Andhra Pradesh. Income and price elasticities were also estimated for major food items using AIDS model. The Primary data were collected from 240 farm households spread over in different regions of Andhra Pradesh. The results revealed that monthly food expenditure for all the income groups was Rs.7450. The per centage of food expenditure was high for meat followed by pulses, cereals, vegetables, oil and fruits for all the income groups. The income elasticities of demand for major food items revealed that cereals had low elasticities for all the income groups when compared to elasticities of livestock and horticulture products such as milk, meat, fruits, vegetables etc. Crop diversification and milch animals rearing should be strengthened for regular income to the farm households which will reduce the food expenditure inequalities among farm households.

Price Volatility and Transmission: A case study of paddy and redgram markets in Karnataka

Price volatility of major agricultural commodities has attracted major attention in the last few years in India as well as in the world food markets. It is often observed that price changes of major staple food items have negative impact on the welfare of the producer farmers as well as consumers. This study tries to explore the nature and causes of the price changes of paddy and redgram in Karnataka. The secondary data of paddy and redgram prices from major markets were compiled and analysed using volatility index and co-integration techniques. Data from major markets by considering time series data for about two decades from2000-2018 were used to understand the trends, volatility and transmission. It was discovered that, prices were not particularly unpredictable, retail prices were more erratic than farm harvest prices, wholesale prices, and minimum support prices in Paddy whereas in redgram, farm harvest prices were comparatively erratic than other prices. The volatility may be related to agricultural production’s intrinsic instability as a result of unexpected and unpredictable exogenous shocks such as weather and poor price and income elasticities. And study on price transmission would help to know, how the prices have transmitted from one market to other market and reveals the relationship between prices over time between the markets considered.

Climate-related hazards and Indian food supply: Assessing the risk using recent historical data

Climate-related hazards can lead to agricultural losses and affect local and wider food supply via food trade. This study estimates the potential for adverse effects of climate hazards on food supply across Indian States and Union Territories (hereafter ‘states’) by quantifying climate hazard risks. Risks were estimated using the most recent data available on hazard presence, vulnerability, and volume of per capita food supply that is exposed to hazards. Historical (2000–2020) climatological and geological data sourced from meteorological stations and satellite imagery were used to estimate the state-level presence of eight climate-related hazards (droughts, forest fires, floods, extreme rainfall, landslides, cyclones, extreme temperatures, sea level rise). For each state and hazard type, we distinguished between risk to food supply produced in the state and the risk to food supply imported from other states. The source of food supply was estimated from a supply and demand balance model for 30 major food items that uses government data from 2011–12. We found that climate hazard risks to food supply vary across states and by hazard type. The largest climate hazard risks to state food supply are in Bihar, Madhya Pradesh, and Assam, where the majority of risk is to locally produced supply. Food supply in each state is at risk to all eight climate hazards via food imports from other Indian states. For 14 states, the climate hazard risk is greater for imported food supply than for locally produced supply. Just five states contribute to more than half of the climate hazard risk in interstate food trade. The findings indicate that climate-related hazards in Indian states could have potentially adverse effects on national food supply, affecting both local production and interstate trade. For policy-makers, these climate hazard risks identify potential priorities for enhancing food system resilience to mitigate impacts on local and national food security.

Evaluation of colour, texture and nutritional properties of Pigmented Rice Based Fermented Steamed Food- Idli

The white rice based fermented steamed food (idli) is a popular and major breakfast food item across the globe. The present study reports the effect of different pigmented rice cultivars (black/red) along with a white rice as check on the colour, texture and nutritional properties of idli. Distribution of different forms of phenolic acids and flavonoids and related antioxidant properties of these products is also examined. The pigmented rice- idli (PRI), although, had lower values for adhesiveness, cohesiveness and resilience, but was nutritionally superior to traditional white rice idli (WRI) in terms of protein(12.74 to 15.53%), amino acids(2.78 to 4.26%), total phenolic acids (0.25 to 0.49 mg/g), total flavonoids(0.36 to0.71 mg/g) content and antioxidant activity(0.16 to 0.46 mg phenolic acid/g). Insoluble bound form of phenolic acids and flavonoids formed the predominant fraction in all these products. PCA scatter plot disclosed MH-idli as a promising product.

The Relative Validity and Reproducibility of Food Frequency Questionnaires in the China Kadoorie Biobank Study

Background: Short versions of qualitative and quantitative food frequency questionnaires (FFQs) are widely used to assess usual food intake. However, fewer studies evaluated their relative validity and reproducibility in the Chinese population. Methods: This study compared 12-day 24-h dietary recalls with qualitative and quantitative FFQs designed by the China Kadoorie Biobank (CKB) study to assess the relative validity. Two FFQs were administered in the second and third seasons and compared to evaluate the reproducibility. Statistical tests included Spearman correlation coefficients, weighted kappa, and cross-classification. Results: A total of 432 participants were eligible after stratifying by age, sex, and four regions. In the validation of qualitative FFQ, adjusted Spearman coefficients were between 0.23 and 0.59, and weighted kappa coefficients ranged from 0.61 to 0.88, except for fresh vegetables. The percentage of correct classification was highest in fresh vegetables and lowest in fresh fruit, but the percentages of extreme classification were below 3.0%. Corresponding Spearman and kappa coefficients for the reproducibility were 0.17–0.56 and 0.62–0.90. Furthermore, the correct classification constituted between 35.6 and 93.3% of all participants. Regarding the relative validity of the quantitative FFQ, Spearman coefficients ranged from 0.14 to 0.69 in addition to dried vegetables and carbonated soft drinks. For items with more than two-thirds of total participants consumed, weighted kappa coefficients were from 0.57 to 0.79; correct classification percentages were between 34.6% and 67.5%. Spearman and kappa coefficients for the reproducibility of the quantitative FFQ were 0.15–0.71 and 0.60–0.86, respectively; correct classification percentages varied from 47.8% to 71.6%. Conclusion: Most food items from the qualitative FFQ showed acceptable or even good relative validity and reproducibility in the CKB study. Likewise, major food items in the quantitative FFQ were valid and reproducible, but poor performances of dried vegetables and carbonated soft drinks indicated the need for modification and validation in future research.

Impact of COVID-19 on food insecurity using multiple waves of high frequency household surveys

In response to the rapid spread of COVID-19, governments across the globe have implemented local lockdowns that have led to increased unemployment and have disrupted local and international transport routes and supply chains. Whilst such efforts aim to slow or stop the spread of the SARS-CoV-2 virus, they have also resulted in increased food insecurity, whether due to reduced incomes or increased food prices. This is the first paper to track food insecurity and its determinants during the pandemic using multi-country and multi-wave evidence. Using data from 11 countries and up to 6 waves of High-Frequency Phone Survey data (household-level surveys) on COVID-19 and its impacts, we use a fixed-effects linear probability model to investigate the socioeconomic determinants of food insecurity during the pandemic for each country using household-level data over multiple waves. We control for socioeconomic characteristics including gender and education of the household head; income and poverty status of the households during the pandemic; safety nets in the form of cash and food assistance; coping strategies adopted by households; and price effects of major food items. Our findings suggest that cash safety nets appear to have been more effective than food in terms of reducing food insecurity during the pandemic; and that those particularly hard hit are female headed-households (highest in Malawi: 0.541, 95% CI 0.516, 0.569; lowest in Cambodia: 0.023, 95% CI 0.022, 0.024), the less educated (highest in Djibouti: − 0.232, 95% CI − 0.221, − 0.244; lowest in Nigeria: 0.006, 95% CI − 0.005, − 0.007), and poorer households (highest in Mali: 0.382, 95% CI 0.364, 0.402; lowest in Chad: 0.135, 95% CI 0.129, 0.142). In line with the existing literature, our results show that, even controlling for income loss and poverty status, those households who had to borrow rather than rely on savings had a higher probability of suffering from food insecurity. Distinct differences in the efficacy of safety nets across the 11 countries, and the differential impact of the pandemic on different groups within societies, suggest in-depth country-specific studies are needed to understand why some countries have coped better than others. Our paper highlights the importance of improving household resilience to future systemic crises, and using evidence-based best practice in the design of relevant policy instruments.

Assessment of Dietary Choline Intake, Contributing Food Items, and Associations with One-Carbon and Lipid Metabolites in Middle-Aged and Elderly Adults: The Hordaland Health Study

BackgroundCholine is an essential nutrient for humans and is involved in various physiologic functions. Through its metabolite betaine, it is closely connected to the one-carbon metabolism, and the fat-soluble choline form phosphatidylcholine is essential for VLDL synthesis and secretion in the liver connecting choline to the lipid metabolism. Dietary recommendations for choline are not available in the Nordic countries primarily due to data scarcity. ObjectivesThe aim of this study was to investigate the dietary intake of total choline and individual choline forms, dietary sources, and the association of total choline intake with circulating one-carbon metabolites and lipids. MethodsWe included 5746 participants in the Hordaland Health Study, a survey including community-dwelling adults born in 1925–1927 (mean age 72 y, 55% women) and 1950–1951 (mean age 48 y, 57% women). Dietary data were obtained using a 169-item FFQ, and choline content was calculated using the USDA Database for Choline Content of Common Foods, release 2. Metabolites of the one-carbon and lipid metabolism were measured in a nonfasting blood sample obtained at baseline, and the association with total choline intake was assessed using polynomial splines. ResultsThe geometric mean (95% prediction interval) energy-adjusted total choline intake was 260 (170, 389) mg/d, with phosphatidylcholine being the main form (44%). The major food items providing dietary choline were eggs, low-fat milk, potatoes, and leafy vegetables. Dietary total choline was inversely associated with circulating concentrations of total homocysteine, glycine, and serine and positively associated with choline, methionine, cystathionine, cysteine, trimethyllysine, trimethylamine-N-oxide, and dimethylglycine. A weak association was observed between choline intake and serum lipids. ConclusionsPhosphatidylcholine was the most consumed choline form in community-dwelling adults in Norway. Our findings suggest that choline intake is associated with the concentration of most metabolites involved in the one-carbon and lipid metabolism.