Based on ancestral knowledge towards health beneficial effect, the current study led to investigate the content of bioactive phytochemical(s) in the scented rice (variety “joha”) grain indigenous to the NER-India and their effects on the enzyme involved in metabolic disorders and cellular glucose uptake efficacy. Results of both α-glucosidase inhibitory activity and the glucose uptake assay-guided fractionation based on column purification followed by spectral data analysis confirmed the presence of two major bioactive compounds as linoleic (ω-6-fatty-acid) and linolenic (ω-3-fatty-acid) acid. Quantitative analysis showed that linolenic acid content in scented rice verities was 2.1±0.3 mg per gram, which is ~1.6 fold higher than its content in non-scented rice variety from the same region. The relative ratio (ω-6/ω-3) of these two essential unsaturated fatty acids content in the scented rice variety seeds was calculated as 23.5±0.6 while, in non-scented rice variety seeds, it was 31.6. Chemical fingerprinting by GCMS and HPLC of methanolic extract of selected rice samples revealed the presence of various fatty-acid along with other volatile and polar compounds. In conclusion the scented rice seeds content a better ratio of two essential unsaturated fatty acid compare to non-scented rice seed, which may be an effective choice as diet towards controlling metabolic disorder.