The urgency of researching the issue of investment and innovation activities of transport and logistics companies in today's changing business environment is due to a significant increase in competition in the transport market. The article examines the main types of innovations that can be implemented by domestic transport and logistics companies, in particular: modernization of the company's fleet, the transition to more economical and environmentally friendly fuels for rolling stock, maintenance of rolling stock in good working order, transportation of goods in multimodal and intermodal communications, use of bar codes, GPS-navigators, systems of tracking the location and movement of goods, telepathic technologies, electronic signatures. The innovative approach is used to address the issues of packaging, handling, loading and unloading, and transportation of non-standard and rare goods. The use of a customer service system allows you to constantly communicate with customers and speed up the response to the request. The implementation of innovations is carried out through investment. Transport and logistics companies can use different sources of funding, which include public, private and foreign investment resources. The main stages of making effective management decisions on the implementation of innovative projects are analyzed. At the preparatory stage, it is important to assess the state and potential of the enterprise, as well as institutional, meso- and macroeconomic factors that may affect the innovation and investment activities of transport and logistics companies. For selected investment projects, their strategic assessment of compliance with business development directions and long-term strategic goals of the company should be carried out. Further, the possibilities of the investment project implementing should be checked, as well as cash flow forecasting for the project should be carried out. At the next stage, based on the analysis of the balance sheet and report on the financial results of the enterprise, the calculation of financial ratios is carried out and a conclusion about the financial capacity of the project is made.The final stage is evaluation of the economic, social and ecologic effectiveness of selected projects. It is substantiated that at the level of the business entity it is necessary to clearly define the priority of investment and innovation development and its impact on performance indicators.