Main Subclusters Research Articles

CLASH-VLT: DISSECTING THE FRONTIER FIELDS GALAXY CLUSTER MACS J0416.1-2403 WITH ∼800 SPECTRA OF MEMBER GALAXIES

ABSTRACT We present VIMOS-Very Large Telescope (VLT) spectroscopy of the Frontier Fields cluster MACS J0416.1-2403 (z = 0.397). Taken as part of the CLASH-VLT survey, the large spectroscopic campaign provided more than 4000 reliable redshifts over ∼600 arcmin2, including ∼800 cluster member galaxies. The unprecedented sample of cluster members at this redshift allows us to perform a highly detailed dynamical and structural analysis of the cluster out to ∼2.2 r 200 (∼4 Mpc). Our analysis of substructures reveals a complex system composed of a main massive cluster (M 200 ∼ 0.9 × 1015 M ⊙ and σ V,r200 ∼ 1000 km s−1) presenting two major features: (i) a bimodal velocity distribution, showing two central peaks separated by ΔV rf ∼ 1100 km s−1 with comparable galaxy content and velocity dispersion, and (ii) a projected elongation of the main substructures along the NE–SW direction, with a prominent sub-clump ∼600 kpc SW of the center and an isolated BCG approximately halfway between the center and the SW clump. We also detect a low-mass structure at z ∼ 0.390, ∼10′ south of the cluster center, projected at ∼3 Mpc, with a relative line-of-sight velocity of ΔV rf ∼ −1700 km s−1. The cluster mass profile that we obtain through our dynamical analysis deviates significantly from the “universal” NFW, being best fit by a Softened Isothermal Sphere model instead. The mass profile measured from the galaxy dynamics is found to be in relatively good agreement with those obtained from strong and weak lensing, as well as with that from the X-rays, despite the clearly unrelaxed nature of the cluster. Our results reveal an overall complex dynamical state of this massive cluster and support the hypothesis that the two main subclusters are being observed in a pre-collisional phase, in agreement with recent findings from radio and deep X-ray data. In this article, we also release the entire redshift catalog of 4386 sources in the field of this cluster, which includes 60 identified Chandra X-ray sources and 105 JVLA radio sources.

Comparative molecular epidemiology of two closely related coronaviruses, bovine coronavirus (BCoV) and human coronavirus OC43 (HCoV-OC43), reveals a different evolutionary pattern

Bovine coronaviruses (BCoVs) are widespread around the world and cause enteric or respiratory infections among cattle. The current study includes 13 samples from BCoVs collected in Normandy during an 11-year period (from 2003 to 2014), 16 French HCoV-OC43s, and 113 BCoVs or BCoVs-like sequence data derived from partial or complete genome sequences available on GenBank. According to a genotyping method developed previously for HCoV-OC43, BCoVs and BCoVs-like are distributed on three main sub-clusters named C1, C2, and C3. Sub-cluster C1 includes BCoVs and BCoVs-like from America and Asia. Sub-cluster C2 includes BCoVs from Europe. Sub-cluster C3 includes prototype, vaccine, or attenuated BCoV strains. The phylogenetic analyses revealed the monophyletic status of the BCoVs from France reported here for the first time. Moreover, BCoV exhibits a relative genetic stability when compared to HCoV-OC43 we previously described from the same region. The numerous recombination detected between HCoV-OC43 were much less frequent for BCoV. The analysis points thus to the influence of different evolutive constraints in these two close groups.

The structure of Abell 1351: a bimodal galaxy cluster with peculiar diffuse radio emission

We aim to review the internal structure and dynamics of the Abell 1351 cluster, shown to host a radio halo with a quite irregular shape. Our analysis is based on radial velocity data for 135 galaxies obtained at the Telescopio Nazionale Galileo. We combine galaxy velocities and positions to select 95 cluster galaxy members and analyse the internal dynamics of the whole cluster. We also examine X-ray data retrieved from Chandra and XMM archives. We measure the cluster redshift, <z>=0.325, the line-of-sight (LOS) velocity dispersion, \sigma_v~1500 km/s, and the X-ray temperature, kT~9 keV. From both X-ray and optical data independently, we estimate a large cluster mass, in the 1--4 $10^{15}$ M$_\odot$ range. We attribute the extremely high value of \sigma_v to the bimodality in the velocity distribution. We find evidence of a significant velocity gradient and optical 3D substructure. The X-ray analysis also shows many features in favour of a complex cluster structure, probably supporting an ongoing merger of substructures in Abell 1351. The observational scenario agrees with the presence of two main subclusters in the northern region, each with its brightest galaxy (BCG1 and BCG2), detected as the two most important X-ray substructures with a rest-frame LOS velocity difference of \Delta v~2500 km/s (in the rest frame) and probably being in large part aligned with the LOS. We conclude that Abell 1351 is a massive merging cluster. The details of the cluster structure allow us to interpret the quite asymmetric radio halo as a `normal' halo plus a southern relic, strongly supporting a previous suggestion based only on inspection of radio and preliminary X-ray data.

The Festuco-Brometea Grasslands on Sandstone and Marl-Clay-Sandstone Substrata in Tuscany (Northern-Central Italy)

Abstract The grasslands dominated by Bromus erectus and/or Brachypodium rupestre cover large areas on sandstone and marl-clay-sandstone substrata (limestone is excluded), of the Apennines and pre-Apennines between Pistoia and Arezzo provinces (Tuscany, Central Italy). Our investigation was focused on to 71 unpublished releves and 45 literature releves from Tuscany and surrounding areas, reporting the original releves of Astragalo monspessulani-Brometum erecti, Centaureo bracteatae-Brometum erecti and Ononido masquillerii-Brometum erecti. The releves were submitted to exploratory multivariate analysis, allowing to detect nine distinct groups. Their consistence was verified by mean of NMDS against Ellenberg/Pignatti indicator values, and CCA constrained against chorotypes and growth forms. Diagnostic species of the definitive groups resulting from the analysis were chosen according to species fidelity, based on the φ coefficient of association. The analysis splits the data set in two main subclusters; the first one (A) includes few releves of low altitude, thermophilous conenoses, interpreted as a transition between the submediterranean aspects of Festuco-Brometea class and other Mediterranean herbaceous and shrubby classes; the second subcluster (B) includes most of the data set and can be clearly subdivided in pioneering, mesoxerophilous communities (B1 and B2a groups) and mesophilous communities (B2b group). The releves of clusters B1 and B2a are referred to Coronillo minimae-Astragaletum monspessulanii association and to three other groups: Plantago argentea-Carex caryophyllea communities, Tragopogon samaritani-Bromus erectus communities, Festuco trachyphyllae-Brometum erecti ass. nova. The mesophilous group (B2b) includes the original Centaureo bracteatae-Brometum erecti and Ononido masquillerii-Brometum erecti releves, together with a slightly differentiated community; due to the non-relevant differences between these grassland types from floristic, ecological and chorological perspectives, we propose herewith to treat them as three subassociations of Centaureo bracteatae-Brometum erecti (typicum, ononidetosum masquillerii and linetosum cathartici). Post-cultural grasslands in human-disturbed areas were also detected. All these communities can be attributed to Polygalo mediterraneae-Bromenion erecti suballiance (Bromion alliance), even if a clear subdivision between the mesoxerophilous pioneer aspects and the more mesophilous and evolved ones can be noted.

The galaxy population of the complex cluster system Abell 3921

We present a spectrophotometric analysis of the galaxy pop. in the area of the merging cluster Abell 3921 at redshift 0.093. We investigate the impact of the complex cluster environment on galaxy properties such as morphology or star formation rate. We combine multi-object spectroscopy from the 2dF spectrograph with optical imaging taken with the ESO WFI. We carry out a redshift analysis and determine cluster velocity dispersions using biweight statistics. Applying a Dressler-Shectman (DS-)test we seek evidence for cluster substructure. Cluster and field galaxies are investigated with respect to [OII] and H{\alpha} equivalent width, SFR and morphological descriptors such as concentration index and Gini coefficient. We study these cluster galaxy properties as a function of clustercentric distance and investigate the spatial distribution of various galaxy types. Applying the DS-test we find a 3rd component (A3921-C) in addition to the two main subclusters (A3921-A and A3921-B) already known. The re-determined mass ratio between the main components A and B is approx. 2:1. Similar to prev. studies of galaxy clusters, we find that a large fraction of the disk galaxies close to the cluster core show no detectable star formation. These are likely systems that are quenched due to ram pressure stripping. We also find quenched spirals at rather large distances of 3 to 4 Mpc from the cluster core. A3921-C might be a group of galaxies falling onto the main cluster components. We speculate that the unexpected population of quenched spirals at large clustercentric radii in A3921-A and A3921-B might be an effect of the ongoing cluster merger: shocks in the ICM might give raise to enhanced ram pressure stripping and at least in part be the cause for the quenching of star formation. These quenched spirals might be an interm. stage in the morphological transformation of field spirals into cluster S0s.

Molecular epidemiological characteristics of HIV-1 B'/C strains in Beijing

To analyze the molecular epidemiological characteristic of HIV-1 B'/C strains prevalent in Beijing. Plasma samples were collected from 200 newly diagnosed HIV-1 B'/C individuals reported during 2006 to 2010 in Beijing. The gag gene fragments were amplified from RNA template extracted from plasma with reverse transcription (RT) and nested polymerase chain reaction (PCR). And the sequences were analyzed by phylogenetic methods and Entropy analysis. A total of 159 sequences were successfully amplified from the gag genes of which 147 was CRF07_BC and 12 CRF08_BC. There were 3 main sub-clusters in CRF07_BC phylogenetic tree and they were named as sub-cluster IDU-Max (89 sequences), sub-cluster IDU-Min (22 sequences) and sub-cluster MSM (34 sequences) based on transmission.No international reference strain was closely related with these three sub-clusters except for one strain identified in Taiwan. All CRF07_BC recombinant strains were remarkable for their low interpatient diversity in gag genes (3.7%, 3.3% and 2.0% for isolates from IDU-Max, IDU-Min and MSM respectively).When compared with sub-cluster IDU-Max, there were 32 and 41 significantly different sites of nucleotide polymorphism compositions in sub-clusters IDU-Min and MSM. This is the first report of describing the existence of three main epidemic sub-clusters in CRF07_BC strains prevalent in Beijing. And IDU-Max sub-cluster is the dominant strain. The CRF07_BC in Beijing are less diverse than other strains and may be derived from a common ancestor.

Genetic Relationships of Cassava Genotypes That are Susceptible or Tolerant to Cassava Brown Streak Disease in Uganda

A total of 99 cassava genotypes whose field reaction to cassava brown streak disease (CBSD) was known, were assayed with 30 simple sequence repeat (SSR) markers to establish their genetic parallels. Two categories of CBSD reaction were considered: CBSD-susceptible genotypes (characterized by > 60% root and foliar CBSD incidence), and CBSD-tolerant genotypes (characterized by < 15% root and foliar CBSD incidence). DNA was extracted from leaf samples using the miniprep method and genotyped using ABI 3730 DNA sequencer. The test genotypes at 0.02 similarity coefficient, CBSD-tolerant and CBSD-susceptible genotypes clustered into 5 main sub-clusters. When data were subjected to principle component analysis (PCA) irrespective of the CBSD reaction grade, the first three principal components accounted for 68% of the total genetic variation. Despite having different number of individuals, the observed heterozygosity (Ho) for CBSD-susceptible (Ho = 0.58) and CBSD-tolerant (Ho = 0.66) were comparable and provide scope for long-term CBSD breeding and/or gene tapping.

Structure of Abell 1995 from optical and X-ray data: a galaxy cluster with an elongated radio halo

Abell 1995 is a puzzling galaxy cluster hosting a powerful radio halo, but it has not yet been recognized as a obvious cluster merger, as usually expected for clusters with diffuse radio emission. We aim at an exhaustive analysis of the internal structure of Abell 1995 to verify if this cluster is really dynamically relaxed, as reported in previous studies. We base our analysis on new and archival spectroscopic and photometric data for 126 galaxies in the field of Abell 1995. The study of the hot intracluster medium was performed on X-ray archival data. Based on 87 fiducial cluster members, we have computed the average cluster redshift <z>=0.322 and the global radial velocity dispersion ~1300 km/s. We detect two main optical subclusters separated by 1.5 arcmin that cause the known NE-SW elongation of the galaxy distribution and a significant velocity gradient in the same direction. As for the X-ray analysis, we confirm that the intracluster medium is mildly elongated, but we also detect three X-ray peaks. Two X-ray peaks are offset with respect to the two galaxy peaks and lie between them, thus suggesting a bimodal merger caught in a phase of post core-core passage. The third X-ray peak lies between the NE galaxy peak and a third, minor galaxy peak suggesting a more complex merger. Simple analytical arguments suggest a merging scenario for Abell 1995, where two main subsystems are seen just after the collision with an intermediate projection angle. The high mass of Abell 1995 and the evidence of merging suggest it is not atypical among clusters with known radio halos. Interestingly, our findings reinforce the previous evidence for the peculiar dichotomy between the dark matter and galaxy distributions observed in this cluster.

Molecular Epidemiology of Staphylococcus epidermidis Clinical Isolates from U.S. Hospitals

The epidemiology of Staphylococcus epidermidis in U.S. hospitals remains limited. This study aimed to address the genetic backgrounds of linezolid-susceptible and -resistant S. epidermidis strains (isolated in 2010), including cfr-carrying strains. In addition, the antimicrobial susceptibility profiles and linezolid resistance mechanisms among clonal lineages were assessed. A total of 71 S. epidermidis isolates were selected, and linezolid-resistant strains were screened for cfr and mutations in 23S rRNA, L3, and L4. All isolates were subjected to multilocus sequence typing (MLST), and the results were analyzed by eBURST. Overall, 27 sequence types (STs) were detected, and ST5 (21.1%) and ST2 (16.9%) predominated. The majority (62/71; 87.3%) of STs belonged to clonal complex 2 (CC2), which was mostly comprised of subclusters CC2-II (41/62; 66.1%) and CC2-I (21/62; 33.9%). Other STs were grouped within CC23 or CC32 or were singletons. CC2-I strains were more likely to display a methicillin (95.2% versus 33.3 to 70.7%), a linezolid (47.6% versus 0.0 to 7.3%), or a multidrug (81.0% versus 33.3 to 36.6%) resistance phenotype. Among linezolid-resistant isolates, cfr was noted only within CC2 strains, and it was detected equally in the CC2-I (3/10; 30.0%) and CC2-II (1/3; 33.3%) subclusters. 23S rRNA mutations (G2576 [seven strains] and C2534 [one strain]) were observed only among CC2-I (8/10; 80.0%) isolates. Strains showing a G2576 alteration also had M156 (7/7; 100.0%) and/or H146 (6/7; 85.7%) L3 modifications. This study provides an overview of the S. epidermidis clonal distribution and reports higher resistance rates among CC2-I strains. The results show that cfr may be acquired and expressed by both CC2 main subclusters, while 23S rRNA mutations appeared more often within CC2-I strains. Interestingly, these 23S rRNA mutants also had L3 alterations, which may act synergistically or in a compensatory manner to minimize the fitness cost while providing survival advantages under selective pressure.

Clusters of PMS stars in the Magellanic Clouds: the case of LH95

AbstractWe present a study of the pre-main-sequence (PMS) population in the stellar association LH95 in the LMC, based on the deepest HST/ACS photometry ever taken in a region of the Magellanic Clouds. This association hosts ~ 2500 PMS stars, distributed in three main subclusters. We isolate this population by subtracting the LMC field, study the reddening distribution which peaks at AV ~ 0.5 mag, and assign masses, down to 0.2 M⊙, to each member using a newly derived conversion of evolutionary models for the LMC metallicity and the ACS photometric system. We derive the first IMF in the subsolar regime ever measured outside our Galaxy, complete down to 0.4 M⊙. It presents a flattening at 1 M⊙, changing its slope from x = 2.05 for intermediate-mass stars to x = 1.05 in the subsolar regime. Correcting for unresolved binarity, it is compatible with the Galactic IMF. We do not find evidence of spatial variations of the IMF in the region. We study the ages of the PMS members using maximum-likelihood methods based on the location of the stars in the color–magnitude diagram with respect to 2D isochrones obtained by applying intrinsic and observational biases (differential extinction, variability, confusion, unresolved binarity) to modeled simple stellar populations. We find a most likely global age of ~ 10 Myr, and demonstrate that neglecting the aforementioned biases leads to an underestimation of the cluster age of up to 50%. We find that the observed luminosity spread is more than twice larger than can be explained by such biases, confirming that the star formation in LH95 lasted a few Myr.

Large-scale mRNA analysis of female skeletal muscles during 60 days of bed rest with and without exercise or dietary protein supplementation as countermeasures

Microgravity has a dramatic impact on human physiology, illustrated in particular, with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for defining efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on the transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n = 8), and the potential beneficial impact of protein supplementation (BRN group, n = 8) and of a combined resistance and aerobic training (BRE group, n = 8). Gene expression profiles were obtained using a customized microarray containing 6,681 muscles-relevant genes. A two-class statistical analysis was applied on 2,103 genes with consolidated expression in BRC, BRN, and BRE groups. We identified 472 and 207 mRNAs whose expression was modified in SOL and VL from BRC group, respectively. Further clustering analysis, identifying relevant biological mechanisms and pathways, reported five main subclusters. Three are composed of upregulated mRNAs involved mainly in nucleic acid and protein metabolism, and two made up of downregulated transcripts encoding components involved in energy metabolism. Exercise countermeasure demonstrated drastic compensatory effects, decreasing the number of differentially expressed mRNAs by 89 and 96% in SOL and VL, respectively. In contrast, nutrition countermeasure had moderate effects and decreased the number of differentially-expressed transcripts by 40 and 25% in SOL and VL. Together, these data present a systematic, global and comprehensive view of the adaptive response of female muscle to long-term atrophy.

Gene expression profiling reveals different pathways related to Abl and other genes that cooperate with c-Myc in a model of plasma cell neoplasia

BackgroundTo elucidate the genes involved in the neoplastic transformation of B cells, global gene expression profiles were generated using Affymetrix U74Av2 microarrays, containing 12,488 genes, for four different groups of mouse B-cell lymphomas and six subtypes of pristane-induced mouse plasma cell tumors, three of which developed much earlier than the others.ResultsUnsupervised hierarchical cluster analysis exhibited two main sub-clusters of samples: a B-cell lymphoma cluster and a plasma cell tumor cluster with subclusters reflecting mechanism of induction. This report represents the first step in using global gene expression to investigate molecular signatures related to the role of cooperating oncogenes in a model of Myc-induced carcinogenesis. Within a single subgroup, e.g., ABPCs, plasma cell tumors that contained typical T(12;15) chromosomal translocations did not display gene expression patterns distinct from those with variant T(6;15) translocations, in which the breakpoint was in the Pvt-1 locus, 230 kb 3' of c-Myc, suggesting that c-Myc activation was the initiating factor in both. When integrated with previously published Affymetrix array data from human multiple myelomas, the IL-6-transgenic subset of mouse plasma cell tumors clustered more closely with MM1 subsets of human myelomas, slow-appearing plasma cell tumors clustered together with MM2, while plasma cell tumors accelerated by v-Abl clustered with the more aggressive MM3-MM4 myeloma subsets. Slow-appearing plasma cell tumors expressed Socs1 and Socs2 but v-Abl-accelerated plasma cell tumors expressed 4–5 times as much. Both v-Abl-accelerated and non-v-Abl-associated tumors exhibited phosphorylated STAT 1 and 3, but only v-Abl-accelerated plasma cell tumors lost viability and STAT 1 and 3 phosphorylation when cultured in the presence of the v-Abl kinase inhibitor, STI-571. These data suggest that the Jak/Stat pathway was critical in the transformation acceleration by v-Abl and that v-Abl activity remained essential throughout the life of the tumors, not just in their acceleration. A different pathway appears to predominate in the more slowly arising plasma cell tumors.ConclusionGene expression profiling differentiates not only B-cell lymphomas from plasma cell tumors but also distinguishes slow from accelerated plasma cell tumors. These data and those obtained from the sensitivity of v-Abl-accelerated plasma cell tumors and their phosphorylated STAT proteins indicate that these similar tumors utilize different signaling pathways but share a common initiating genetic lesion, a c-Myc-activating chromosome translocation.

XMM‐NewtonObserves Cl J0152.7−1357: A Massive Galaxy Cluster Forming at Merger Crossroads atz = 0.83

We present an analysis of a 50 ks XMM-Newton observation of the merging galaxy cluster Cl J0152.7-1357 at z = 0.83. In addition to the two main subclusters and an infalling group detected in an earlier Chandra observation of the system, XMM-Newton detects another group of galaxies possibly associated with the cluster. This group may be connected to the northern subcluster by a filament of cool (1.4 keV) X-ray-emitting gas and lies outside the estimated virial radius of the northern subcluster. The X-ray morphology agrees well with the projected galaxy distribution in new K-band imaging data presented herein. We use detailed spectral and imaging analysis of the X-ray data to probe the dynamics of the system and find evidence that another subcluster or group has recently passed through the northern subcluster. Cl J0152.7-1357 is an extremely dynamically active system, with mergers at different stages occurring along two perpendicular merger axes.

Study of the Structure of the Coma Cluster Based on a Hierarchical Force Clustering Method

Six subclusters in the Coma cluster have been selected on the basis of a hierarchical clustering method that takes the gravitational interaction among galaxies into account. Of these, three main subclusters around the galaxies NGC 4889, NGC 4874, and NGC 4839 have been singled out. We have used the objective statistical criterion applied by Vennik and Anosova in studies of close groups of galaxies to evaluate each member included in a subcluster with a high probability. Galaxies with a significant deficit of hydrogen HI, including objects from the Bravo-Alfaro list,have been identified with members of the subclusters, with the greatest number of them in the subclusters around NGC 4874 and NGC 4839. A quantitative estimate of the hydrogen deficit using the HI index in the RCG3 catalog reveals a statistically significant excess value for those galaxies that are members of the subclusters compared to the field galaxies with a hydrogen deficit in the studied area of Coma cluster. A substantial number of the spiral galaxies with a hydrogen deficit in the subclusters turned out to be radio galaxies as well.

Molecular analyses on the genetic diversity and inheritance of (−)-α-bisabolol and chamazulene content in tetraploid chamomile ( Chamomilla recutita (L.) Rausch.)

The study aims to analyse (i) the genetic diversity of cultivated chamomile germplasm, (ii) to possibly discriminate chemotypes and (iii) to develop molecular markers for the (−)-α-bisabolol and chamazulene content by using RAPD and AFLP marker analysis. By analysing di- and tetraploid chamomile ( Chamomilla recutita (L.) Rausch.) accessions using 18 RAPD primers and 16 EcoRI + 3/ MseI + 3 AFLP primer combinations genetic similarity (GS D) was estimated in a range of 0.68–0.95 (RAPDs) and 0.74–0.88 (AFLPs), respectively. UPGMA-cluster analysis revealed two main groups and additional sub-clusters according to the origin of germplasms but not to the phytochemical composition. In lines selected for the development of segregating populations for marker development a high level of genetic homogeneity within (GS D ≈ 0.9; H′ ≈ 0.1) but diversity between lines (GS D ≈ 0.7; H′ ≈ 0.5) was detected which facilitated the selection of contrasting parental genotypes with respect to (−)-α-bisabolol and chamazulene content as well as genome composition. Segregation analysis of 841 F 2-individuals revealed a monogenic recessive mode of inheritance of the (−)-α-bisabolol and chamazulene content. By bulked segregant analysis three AFLP-markers linked to the (−)-α-bisabolol locus and one RAPD and 17 AFLP markers for the chamazulene content were detected. At least the markers for the chamazulene content are suited for pre-flowering marker based selection procedures.

XMM observation of the dynamically young galaxy cluster CL 0939+4713

We present an XMM observation of the distant galaxy cluster CL 0939+4713. The X-ray image shows pronounced substructure, with two main subclusters which have even some internal structure. This is an indication that the cluster is a dynamically young system. This conclusion is supported by the temperature distribution: a hot region is found between the two main subclusters indicating that they are at the beginning of a major merger, and that they will probably collide in a few hundreds of Myr. The intra-cluster gas of CL 0939+4713 shows inhomogeneities in the metal distribution, with the optically richer subcluster having a higher metallicity.

Psychrobacters and Related Bacteria in Freshwater Fish

Three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (Salmo trutta and Esox lucius) and farmed (Oncorhynchus mykiss) freshwater fish. Using diagnostic tables and computer-assisted identification, the isolates were Psychrobacter (64 strains), Acinetobacter (24 strains), Moraxella (6 strains), Chryseobacterium (5 strains), Myroides odoratus (2 strains), Flavobacterium (1 strain), Empedobacter (1 strain), and unidentified (3 strains). Overall similarities of all strains were determined for 108 characters by numerical analysis (simple matching coefficient of similarity [S] and clustering by unweighted pair group average linkage [UPGMA]). At the 77% similarity level, 92 strains formed nine major clusters (3 or more strains) and four small clusters (2 strains). Cluster 1 (25 isolates divided into two main subclusters) could be assigned to Psychrobacter phenylpyruvicus, clusters 2 and 3 (26 isolates) were designated as Psychrobacter immobilis, and clusters 4 (3 isolates) and 7 (4 isolates) were identified as Psychrobacter urativorans and Psychrobacter spp., respectively. Clusters 5 (five isolates), 6 (three isolates), and 9 (five isolates) were labeled as Acinetobacter spp., Acinetobacter johnsonii, and Acinetobacter lwoffii, respectively. Cluster 8 (12 isolates), with a high resemblance to Thornley's phenon 4 (a heterogeneous group of bacteria isolated from poultry and related to Acinetobacter), remained unnamed. The restriction pattern was identical for strains grouped into clusters 2 and 3 (P. immobilis) but was different for the remaining Psychrobacter isolates. A large proportion of isolates belonging to the family Moraxellaceae were closely related. Psychrobacters and A. johnsonii were present in freshly caught fish and river water. In the latter stages of storage, P. phenylpyruvicus and acinetobacters tended to decrease, whereas P. immobilis increased.

A Three‐dimensional Wavelet Analysis of Substructure in the Coma Cluster: Statistics and Morphology

Evidence of clustering within the Coma Cluster is found by means of a multiscale analysis of the combined angular-redshift distribution. We have compiled a catalog of 798 galaxy redshifts from published surveys of the region of the Coma Cluster. We examine the presence of substructure and of voids at different scales ranging from ~1 to ~16 h-1 Mpc, using subsamples of the catalog ranging from cz = 3000 km s-1 to cz = 28,000 km s-1. Our substructure detection method is based on the wavelet transform and on segmentation analysis. The wavelet transform allows us to find structures at different scales, and the segmentation method allows a quantitative statistical and morphological analysis of the sample. From the whole catalog, we select a subset of 320 galaxies, with redshifts between cz = 5858 km s-1 and cz = 8168 km s-1, which we identify as belonging to the central region of Coma and upon which we have performed a deeper analysis, on scales ranging from 180 h-1 kpc to 1.44 h-1 Mpc. Our results are expressed in terms of the number of structures or voids and their sphericity for different values of the threshold detection and at all the scales investigated. According to our analysis, there is strong evidence of multiple hierarchical substructure, on scales ranging from a few hundreds of kiloparsecs to about 4 h-1 Mpc. The morphology of these substructures is rather spherical. On a scale of 720 h-1 kpc we find two main subclusters that have been found before, but our wavelet analysis shows even more substructures, whose redshift position is approximately marked by the following bright galaxies: NGC 4934 and 4840, NGC 4889, NGC 4898 and 4864, NGC 4874 and 4839, NGC 4927, and NGC 4875.

The phylogeny of the spirochetes

Summary A number of species of Spirochaeta, Treponema, Leptospira and Borrelia have been characterized by the method of ribosomal RNA oligonucleotide cataloging, in order to determine their phylogenetic relationships. The group so formed is phylogenetically very deep, with a major division occurring between the leptospiras on the one side and the remaining spirochetes on the other. At a comparably deep level the greater group also encompasses the recently recognized group of obligate anaerobic halophiles. The main cluster within the greater group comprises three sublines: (1) that containing the bulk of the spirochetes and treponemes examined, (2) that represented by B. hermsii, and (3) that represented by T. hyodysenteriae. The first of these in turn divides into two main subclusters, represented by S. halophila on the one hand and S. stenostrepta on the other.

Application of PCR-Based DNA Fingerprinting Techniques for the Genetic Differentiation of Vibrio cholerae Non-01/Non-0139 Isolates from Kuching, Sarawak

Fifty-four Vibrio cholerae non-01/non-0139 isolates were evaluated for their genetic diversity via randomly amplified polymorphic DNA-PCR (RAPD-PCR), enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and BOX-PCR assays. Based on the various PCR fingerprints, the V. cholerae isolates were successfully differentiated into 39 types by RAPD-PCR, 43 types by ERIC-PCR, and 38 types by the BOX PCR, with the overall average polymorphic distances observed to be at 0.593, 0.527 and 0.504, respectively. The Simpson’s index of diversity of the isolates based on the fingerprinting analyses indicated that these DNA fingerprinting methods have high discriminatory power 0.986 (RAPD-PCR), 0.992 (ERIC-PCR) and 0.983 (BOX-PCR), and could be used independently or as supplements to other methods for the epidemiological investigation of the V. cholerae from water and seafood sources. The dendrograms constructed also showed that the V. cholerae isolates were clustered into several main clusters and sub-clusters, suggesting that different strains were circulating in the water environment and in the seafood sources. We conclude that molecular genotyping of V. cholerae isolates from surface water and seafood samples in Kuching, Sarawak (Malaysia) enabled high level observation of clonal diversity within V. cholerae isolates, and is directly applicable for the molecular epidemiological studies of the V. cholerae isolates.