Currently, heavy metal-contaminated groundwater is an environmental concern. This study investigated the use of bamboo biochar, chitosan-impregnated biochar, and iron-impregnated biochar for arsenic, iron, and manganese removal from groundwater. Isotherms of arsenic, iron, and manganese adsorption by bamboo derived biochar were compared with those of commercial activated carbon in simulated groundwater composed of single and trinary heavy metal solutions. The binding of heavy metals by virgin and loaded bamboo biochar and activated carbon was also investigated by sequential extraction. Chitosan and iron-impregnated biochar had enhanced arsenic adsorption, but these sorbents turned the pH of solution acidic, while it was alkaline for activated carbon. Adsorption equilibrium times of arsenic and iron were faster for single than trinary heavy metal systems because less ion competition occurred at active sites. The Langmuir model fitted the adsorption data well. The maximum adsorption capacities of arsenic, iron, and manganese by bamboo biochar in trinary heavy metal system were 2.2568, 0.6393, and 1.3541 mg g−1, respectively. The main mechanism for arsenic removal was precipitation with iron. Bamboo biochar bound iron in organic and sulfide fractions and manganese with iron-oxide. Bamboo biochar can replace activated carbon as a more efficient and sustainable carbonaceous sorbent material for removal of mixed heavy metals from groundwater within acceptable pH ranges.