Background: Migrant mothers’ knowledge, attitude and practice (KAP) are predictors for children’s vaccination status. Mothers are the main decision makers for childhood health. The objective of this study was to develop a reliable and valid questionnaire to assess the immunization KAP of migrant mothers. Methods: A cross-sectional survey was conducted among migrant mothers by using a questionnaire which was developed by all co-authors according to the objective of this study. Internal consistency was assessed by using Cronbach’s α coefficient. The test-retest reliability was assessed by using Spearman’s rank correlation coefficient. The construct validity was determined through the known-group validity technique. Kruskal-Wallis and Mann-Whitney tests were applied to examine the disparities of immunization KAP of migrant mothers with different socialdemographic characteristics. Results: The mean ± standard deviation (SD) of the knowledge score, attitude score and practice score were 7.8 ± 1.4, 7.2 ± 2.2, 6.2 ± 2.3, respectively. Good internal consistency was found for KAP items with Cronbach’s α coefficient of 0.881, 0.755 and 0.783, respectively. The test-retest reliability showed a satisfactory reliability with a Spearman’s rank correlation coefficient value of 0.817. Migrant mothers with ‘fully immunized’ children showed better KAP scores than mothers who did not, suggesting a good construct validity. Mothers’ age, maternal education level, number of children in household, latest continuous living time, and monthly household income per capita were significantly associated with the immunization KAP among migrant mothers. Conclusions: This study showed an optimal reliability and validity of the questionnaire for measuring the KAP on immunization among migrant mothers in Yiwu and therefore this version can be used in future research.
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