Macular Pigment Optical Density Research Articles

Evaluation of Macular Pigment Optical Density in Healthy Eyes Based on Dual-Wavelength Autofluorescence Imaging in South Indian Population.

PurposeTo estimate macular pigment optical density (MPOD) values across different age groups in the South Indian population across various spatial profiles using dual-wavelength autofluorescence.MethodsSixty eyes of 31 healthy subjects underwent MPOD measurement with Spectralis HRA+OCT. The average MPOD and macular pigment optical volume (MPOV) at 1°, 2°, and 6° radii, the mean MPOD in the classical Early Treatment Diabetic Retinopathy Study (ETDRS) grid, and the spatial profiles of two different age groups across 12 plots covering the radial sectors were recorded.ResultsThe mean age was 39.1 ± 12.7 years. The mean MPOD and MPOV values were 0.38 ± 0.11 and 787.95 ± 225.13 at 1° eccentricity, 0.23 ± 0.08 and 2000 ± 708.24 at 2° eccentricity, and 0.05 ± 0.02 and 4335 ± 2007.71 at 6° eccentricity, respectively. In the ETDRS grid, the mean MPOD was found to be highest in the central sector and lowest in the inferior peripheral ring. We also found that along the radial sectors the lower quadrants tended to have low MPOD as compared to the upper quadrants. Subjects 40 years of age or older had significantly higher averaged MPOD in certain areas (–15° to 15° and 75° to 105°) along the radial sectors than subjects less than 40 years of age.ConclusionsThis study establishes a reference value for future studies of diseased eyes in the South Indian population.Translational RelevanceOur study is unique in that it reports MPOD among the South Indian population across different age groups, as well as the distribution of MPOD in all nine zones of the classical ETDRS grid and various spatial profiles covering the 30° radial sectors centered on the fovea.

Changes in Macular Pigment Optical Density and Visual Acuity after Lutein Supplementation in Adults in Their 20s

Changes in Macular Pigment Optical Density and Visual Acuity after Lutein Supplementation in Adults in Their 20s

Effect of an antioxidant supplement containing high dose lutein and zeaxanthin on macular pigment and skin carotenoid levels

The effect of a high dose lutein/zeaxanthin supplement on macular pigment optical density (MPOD) and skin carotenoid (SC) levels in healthy subjects was investigated. This is a prospective, single-arm, open-label study. Subjects were 16 Japanese, age 26–57 years. Subjects took a supplement containing 20 mg/day of lutein, 4 mg/day of zeaxanthin, and other antioxidants (vitamin C, vitamin E, zinc, copper) for 16 weeks. MPOD levels were measured by a two-wavelength autofluorescence imaging technique. SC levels were measured by reflection spectroscopy. Total volume of MPOD within 9° eccentricity significantly increased by week 8 and continued to increase until week 16 (p < 0.0001, two-way factorial ANOVA). The increase rate of MPOD was significantly higher in subjects with body mass index (BMI) less than 25 kg/m2 (n = 13) compared to those of 25 kg/m2 and higher (n = 3). SC levels increased significantly by week 4 and continued to increase until week 16 (p < 0.0001, two-way factorial ANOVA). All subjects completed the study without any serious adverse events. These results demonstrated the effectiveness of a high dose lutein/zeaxanthin supplement for MPOD volume and SC levels without serious adverse events.

Feasibility Study of a Docosahexaenoic Acid-Optimized Nutraceutical Formulation on the Macular Levels of Lutein in a Healthy Mediterranean Population

Introduction: Macular pigment optical density (MPOD) plays a pivotal role in maintaining macular structure and functioning. Research shows that daily consumption of lutein reduces the risk of eye diseases such as age-related macular degeneration. Objective: This study analyzes the influence of a supplementation containing lutein and antioxidant vitamins either with or without docosahexaenoic acid (DHA), with the main objective of identifying MPOD changes in both eyes at the end of the follow-up using the Visucam®retinograph. The secondary end point was to determine variation in the lutein and DHA levels in plasma and red blood cell membranes (RBCMs), respectively. Methods: One hundred healthy participants (200 eyes) aged 40–70 years (mean age 49.3 years, SEM = 13.7) were randomized in a 1:1 ratio to receive daily one of the following supplements for 3 months: lutein group (LT-G, n = 49) and lutein plus DHA group (LT/DHA-G, n = 51). The MPOD was measured at baseline and end of the follow-up by retinography (Visucam®retinograph). Lutein in plasma was determined by HPLC, and DHA in RBC membranes was analyzed by gas chromatograph/mass spectrometer. Results: From baseline, MPOD showed significantly higher values in the LT/DHA-G than in the LT-G at the end of the study (p < 0.0001). Significantly higher lutein in plasma (p < 0.0001) and DHA (p < 0.0001) levels in the RBC membrane were seen in the LT/DHA-G than in the LT-G at the 3-month follow-up. Conclusion: Lutein supplementation improves MPOD in healthy subjects from a Mediterranean population being significantly increased in the presence of DHA. Therefore, our findings highlight the relevance of the adjunctive role of DHA for better lutein availability.

Auditory and Visual Working Memory Are Differentially Related to Carotenoid Status in Preadolescent Children

Abstract Objectives Carotenoids are plant pigments known to deposit in neural tissues including the hippocampus, a brain substrate that supports several memory forms. However, there is a dearth of knowledge regarding carotenoid status and working memory function in children. Accordingly, this study aimed to understand the relationship between macular and skin carotenoids to visual and auditory working memory (WM) function. Methods Seventy preadolescent children (7–12 years, 32 males) were recruited from the East-Central Illinois area. Auditory working memory was assessed using the story recall subtest of the Woodcock-Johnson IV Test of Cognitive Abilities. A subsample (N = 61, 27 males) completed a visual working memory task and reaction time was quantified to determine speed of memory processing at set sizes of 1 to 4 items. Macular pigment optical density (MPOD) was assessed using customized heterochromatic flicker photometry. Skin carotenoids were assessed using reflection spectroscopy (Veggie Meter). Hierarchical linear regressions were conducted to assess the relationship between carotenoid status and WM function, while controlling for age, sex, income, and whole-body % fat (DXA). Results Auditory WM was positively associated with skin carotenoids (b = 0.263, P = 0.039) but not MPOD (b = −0.044, P = 0.380). In contrast, MPOD was significantly associated with faster visual WM speed at set size 3 (b = −0.253, P = 0.039) and trending at set sizes of 1 (b = −0.225, P = 0.051), 2 (b = −0.171, P = 0.121), and 4 (b = −0.230, P = 0.055). Interestingly, skin carotenoids were not related to visual WM performance at either set size (all P’s &amp;gt; 0.300). Conclusions These results indicate that auditory and visual WM may be differentially related to carotenoids. While skin carotenoids encompass all carotenoids consumed in diet, lutein and zeaxanthin are the only carotenoids which deposit in the macula. Given that MPOD was only related to visual WM, this suggests lutein plays a larger role in these neural functions relative to auditory WM. Interestingly, MPOD's relationship with visual WM increased in strength with the more difficult trial type (i.e., increasing set size), indicating MPOD is related at higher levels of WM capacity. Funding Sources This study was funded by the Egg Nutrition Center.

Effects of Goji Berry Intake on Macular Pigment Optical Density and Skin Carotenoids in Healthy Adults

Abstract Objectives Zeaxanthin (Z) and lutein (L) are xanthophyll carotenoids that function in part as macular pigments. Goji berries (Lycium Barbarum) are rich in zeaxanthin, and contain lutein, β-carotene, and β-cryptoxanthin. High intakes of Z and L have been associated with reduced risk of age-related macular degeneration. We assessed the impact of 3 months of 5 times per week intake of 28 g goji berry (GB), or a supplement providing 4 mg of Z and 6 mg of L (ZL) on macular pigment optical density (MPOD) and skin carotenoids (SC) in healthy adults aged 45–65 y old. Methods A randomized, open-labeled, parallel-arm pilot study was conducted at UC Davis. Study visits at baseline (SV1), and at 45 d (SV2) and 90 d (SV3) used heterochromatic flicker photometry to measure MPOD at 0.25, 0.5, 1, and 1.75 retinal eccentricities (RE), and Raman spectroscopy for SC. Between group changes within each treatment group were assessed with one-way Repeated Measures ANOVA with the Holm-Sidak post-hoc method. Results To date, 6 males and 13 females have been assessed. SC was not significantly different between the groups at SV1. The GB group showed a significant increase in SC at 45 d and 90 d compared to baseline (350.6 ± 138.4 SV1 vs. 400.6 ± 131.5 SV2 vs. 409.6 ± 156.6 SV3, P &amp;lt; 0.001, n = 11). No significant changes in SC were found in the ZL group (P = 0.2, n = 8). MPOD did not differ between any RE at any time point for either group. MPOD was positively and significantly correlated (Pearson's or Spearman's correlation) with SC at 0.25 (r = 0.49, P = 0.004), 0.5 (rho = 0.48, P = 0.005) and 1 (rho = 0.45, P = 0.008) RE in the GB group. SC was also significantly correlated with MPOD in the ZL group at 0.25 (rho = 0.43, P = 0.038) and 1 (r = 0.41, P = 0.047) RE. Conclusions We report preliminary results that goji berry intake increases skin carotenoids. These data confirm that Raman spectroscopy is a useful tool for the relationship of skin carotenoids with dietary carotenoid intake, as well as changes in skin carotenoids with macular pigment optical density. Funding Sources UC Davis, Department of Nutrition.

Macular Carotenoids Are Associated with Neural Efficiency in Children

Abstract Objectives Macular pigmentation has been previously related to benefits for behavioral and neuroelectric aspects of selective attention across the lifespan. The relationship between accumulation of carotenoids beyond the central nervous system and selective attention is less understood, particularly amongst children. Therefore, this study aimed to assess the differential impacts of retinal and skin carotenoid accumulation and behavioral and neuroelectric indices of selective attention. Methods Children between 7–12 years (N = 60) participated in the study. Macular pigment optical density (MPOD) was assessed using heterochromatic flicker photometry and skin carotenoids were assessed using reflection spectroscopy at the fingertip using the Veggie meter. Body Mass Index adjusted for age and sex (BMI%) and general intelligence as assessed using the Woodcock Johnson IV test were used as covariates. Behavioral performance (accuracy and reaction time) and neuroelectric indices (event-related brain potentials [ERPs]) of attentional inhibition were assessed during a modified Eriksen Flanker task. Specifically, amplitude of the P3 waveform was used to index attentional resource allocation. Results After controlling for covariates, MPOD was selectively associated with lower peak amplitude of the P3 waveform during congruent (β = −0.36, P = 0.01) and incongruent task trials (β = −0.39, P &amp;lt; 0.01). No significant relationships were observed between the P3 and skin carotenoids. Skin carotenoids were associated with higher accuracy on the incongruent trials of the Flanker task (β = 0.36, P = 0.02), while no relationships were observed between MPOD and behavioral performance. Conclusions In this study, we were able to replicate previous findings demonstrating that higher accumulation of retinal carotenoids is associated with neural efficiency during a selective attention task. Skin carotenoids were associated with behavioral performance but were not related to neuroelectric indices of selective attention, suggesting site-specific benefits of carotenoid accumulation on cognitive health in childhood. Funding Sources This work is funded by the Egg Nutrition Center.

Differential Relationships Between Serum Xanthophylls and Macular Pigment and Retinal Morphology

Abstract Objectives Lutein and zeaxanthin are xanthophyll carotenoids that comprise macular pigment in the eye and disproportionately accumulate in brain tissue. While the link between serum xanthophylls and macular pigment has received considerable investigation, comparatively less is known regarding the relationship between circulating xanthophylls and retinal morphology. This knowledge gap is significant since deterioration of the morphometric measures of the retina (e.g., retinal nerve fiber layer, RNFL) is a feature of age-neurodegenerative diseases. This project aimed to understand the relationship between serum xanthophylls and retinal morphology. Methods Subjects included 84 individuals (30 males) with overweight and obesity (BMI ≥ 25 kg/m2) between 25–45 years. Macular pigment optical density (MPOD) was measured using heterochromatic flicker photometry. Optical coherence tomography (OCT) was used to assess RNFL, ganglion cell layer (GCL), and inner plexiform layer (IPL). Venous blood draws were used to assess serum lutein and zeaxanthin. Covariates assessed included demographic factors, adiposity (DXA), and dietary intake (7d food records). Results Serum lutein (r = 0.32, P &amp;lt; 0.01) and zeaxanthin (r = 0.23, P = 0.01) concentrations were associated with greater MPOD. There were no significant associations between serum xanthophyll concentrations and morphological values. Similarly, there was no influence of adiposity or dietary lutein and zeaxanthin on retinal morphological measures. However, overall diet quality, assessed using Healthy Eating Index (HEI) was positively associated with IPL (r = 0.35, P &amp;lt; 0.01) and GCL (r = 0.32, P &amp;lt; 0.01) thickness. Conclusions Although circulating carotenoids were associated with macular pigment, this relationship did not extend to morphological measures in the retina. However, overall dietary quality was significantly associated with retinal morphological measures. These findings suggest that macular pigment and retinal morphology may be influenced by nutritional factors. Funding Sources Hass Avocado Board.

Efficacy Evaluation and Dose Prediction of a Novel Botanical Formula on Eye Fatigue and Dry Eye to Provide a Personalized Nutrition Solution

Abstract Objectives With the frequent use of video display units, eye fatigue is more and more common globally. We aimed to evaluate the protective effect of a novel botanical combination of lutein ester, zeaxanthin, extracts of black currant, chrysanthemum and goji berry on adults with eye fatigue in a randomized controlled trial, and predict personalized dose for each individual in real-life consumption. Methods 360 participants were randomized to receive our formula with 3 different doses (containing 6 mg, 10 mg, and 14 mg of lutein, respectively) or placebo once daily for 90 days. Each participant had a total of 3 visits at baseline (V1), 45 days (V2) and 90 days (V3) throughout the study. Results The formula intervention improved individual score of eye fatigue symptoms, including eye soreness, photophobia, blurred vision, dry eye, foreign body sensation and tearing. Compared with placebo, the formula with all 3 doses significantly decreased the total score of eye fatigue symptoms and increased the visuognosis persistence time at both V2 and V3. For Schirmer test, the formula with 10 mg and 14 mg of lutein improved the tear secretion at V3. For Keratography, first tear break time, average tear break time and tear meniscus height were significantly increased after formula intervention. The intervention of formula with all 3 doses significantly increased macular pigment optical density at V2 and V3, while optical coherence tomography showed that there was no significant difference in retinal thickness and retinal volume among all groups at both visits. Different machine-learning algorithms were used to predict personalized dose based on the eye-related indexes and other features, such as anthropometrics, physical activity, dietary habit, and blood biomarkers under 3 different dosages. Among them, XGBoost performed best with R = 0.649 for training set, R = 0.638 for test set, and R = 0.685 for external validation set. Conclusions The formula improves eye fatigue, dry eye, and macular function without changing the structure, providing a nutritional alternative strategy. XGBoost could successfully predict dose to provide a personalized nutrition solution. Funding Sources This study was supported by National Key R&amp;D Program of China and Nutrition Research Foundation of Chinese Nutrition Society.

Efficacy of Commercially Available Nutritional Supplements: Analysis of Serum Uptake, Macular Pigment Optical Density and Visual Functional Response.

Purpose: To compare the change in serum carotenoids, macular pigment optical density (MPOD) and visual function with the intake of two commercially available nutritional supplements. Methods: Participants were given a 24-week supply of a lipid-based micronized liquid medical food, Lumega-Z™ (LM), containing 28 mg of the macular carotenoids lutein (L), zeaxanthin (Z) and meso-zeaxanthin (MZ), or given PreserVision™ AREDS 2 Formula (gel-caps; PV) containing 12 mg of the macular carotenoids L and Z, but no reported MZ. Serum levels of L, Z and MZ were obtained at baseline and after 12 weeks. Macular pigment optical densities (MPOD) and visual function were assessed at baseline and after 24 weeks. Results: Average blood serum concentrations of L, Z and MZ in the two groups at baseline were similar. The increases in L, Z and MZ were 0.434, 0.063 and 0.086 µmol/L vs. 0.100, 0.043 and 0.001 µmol/L, respectively, in the LM vs. PV group. From baseline to week 24, average MPOD in the LM-group increased by 0.064 from 0.418 to 0.482, whereas in the PV-group, it was essentially unchanged (0.461 to 0.459;). Although log-contrast sensitivity was improved in all groups under three conditions (photopic, mesopic and mesopic with glare), the change in log-contrast sensitivity was not statistically significant. Conclusion: Despite only a 2.3-fold higher carotenoid concentration than PV, LM supplementation provides approximately 3–4-fold higher absorption, which leads to a significant elevation of MPOD levels.

Macular Pigment Optical Density and Photoreceptor Outer Segment Length as Predisease Biomarkers for Age-Related Macular Degeneration.

To explore predisease biomarkers, which may help screen for the risk of age-related macular degeneration (AMD) at very early stages, macular pigment optical density (MPOD) and photoreceptor outer segment (PROS) length were analyzed. Thirty late AMD fellow eyes, which are at high risk and represent the predisease condition of AMD, were evaluated and compared with 30 age-matched control eyes without retinal diseases; there was no early AMD involvement in the AMD fellow eyes. MPOD was measured using MPS2® (M.E. Technica Co. Ltd., Tokyo, Japan), and PROS length was measured based on optical coherence tomography images. MPOD levels and PROS length in the AMD fellow eyes were significantly lower and shorter, respectively, than in control eyes. MPOD and PROS length were positively correlated in control eyes (R = 0.386; p = 0.035) but not in AMD fellow eyes. Twenty (67%) AMD fellow eyes met the criteria of MPOD < 0.65 and/or PROS length < 35 μm, while only five (17%) control eyes did. After adjusting for age and sex, AMD fellow eyes more frequently satisfied the definition (p < 0.001; 95% confidence interval, 3.50–60.4; odds ratio, 14.6). The combination of MPOD and PROS length may be a useful biomarker for screening predisease AMD patients, although further studies are required in this regard.

Исследование зависимости между толщиной хориоидеи и оптической плотностью макулярного пигмента у молодых пациентов с миопической рефракцией

Исследование зависимости между толщиной хориоидеи и оптической плотностью макулярного пигмента у молодых пациентов с миопической рефракцией

Effects of Lutein Supplementation in Japanese Patients with Unilateral Age-Related Macular Degeneration: The Sakai Lutein Study

This prospective randomized double-masked study investigated the effects of 20 mg lutein supplementation with two different capsules (beeswax or glycerol fatty acid esters) for 6 months on the fellow eyes of 39 Japanese patients with unilateral age-related macular degeneration, and assessed the factors associated with baseline plasma lutein concentration via lifestyle interviews. Macular pigment optical density (MPOD), determined with the two-wavelength autofluorescence method, increased over time in the beeswax group (ANOVA, p = 0.0451), although the increase from 3 months to 6 months was only marginally significant. No significant increase was observed in the glycerol fatty acid esters group (ANOVA, p = 0.7396). Plasma lutein concentrations significantly increased at 3 and 6 months from baseline in both groups (both p < 0.01). In a multiple regression model, age was negatively associated with higher plasma lutein concentration (p = 0.0305), while consumption of green vegetables was positively associated with baseline plasma lutein concentration (p = 0.0322). In conclusion, a significant increase in MPOD was not fully confirmed with 6 months intake duration despite a significant increase in plasma lutein concentrations. Consumption of green vegetable was confirmed to be associated with plasma lutein concentration after adjusting for other potential factors including age.

Comparison of Macular Pigment Optical Density in Glaucoma Patients and Healthy Subjects - A Prospective Diagnostic Study.

PurposeTo evaluate the relationship between macular pigment optical density (MPOD) and glaucoma.MethodsForty-three patients with an established glaucoma diagnosis (25 females, 18 males, mean age 70 (range 34–84)) and 43 healthy controls (28 females, 15 males, mean age 62 (range 30–87)) were included in this prospective diagnostic case-control study. All subjects underwent detailed eye examination including ophthalmoscopy, best-corrected visual acuity, biomicroscopy, measurement of the axial length of the eye, objective refraction, lens status, central foveal thickness on spectral-domain optical coherence tomography (SD-OCT). In all glaucoma patients, a visual field assessment and a measurement of the retinal nerve fibre layer (RNFL) on SD-OCT were done. MPOD was determined using the macula pigment module of the Spectralis HRA+OCT (Heidelberg Engineering GmbH, Heidelberg, Germany) at 0.51°, 1.02° and 1.99° retinal eccentricity using two-wavelength autofluorescence imaging.ResultsIn the glaucoma group, the median of the visual field mean defect was 5.1 db (quartiles 3.0 and 13.5) and the mean RNFL-thickness global was 65.9 µm (SD ± 16.1). Median MPOD measured at 0.51°, 1.02° and 1.99° retinal eccentricity in the glaucoma group was 0.42 DU, 0.34 DU and 0.13 DU, in the control group 0.40 DU, 0.35 DU and 0.12 DU respectively. There was no statistically significant difference of median MPOD between glaucomatous and control eyes (p=0.510, 0.735, 0.481). No significant relation between MPOD at 1.02 retinal eccentricity and the presence of glare symptoms was found (p=0.948). However, age seems to correlate with median MPOD measured at 1.02 retinal eccentricity (p=0.017).ConclusionThere was no evidence for lower MPOD levels in our glaucoma patients; lower MPOD was not related to the presence of glare symptoms. However there seems to be a positive correlation between age and MPOD at 1.02° retinal eccentricity. To further investigate the relation between glare reported by glaucoma patients and glare disability linked to lower MPOD levels, additional studies are necessary that include both detailed inquiry of the quality of glare and a glare quantification and precise analysis of MPOD levels in glaucoma patients.

Correlation between Macular Pigment Optical Density and Neural Thickness and Volume of the Retina.

Macular pigment (MP), which is composed of lutein/zeaxanthin/mezo-zeaxanthin, is concentrated in the central part of the retina, the macula. It protects the macula by absorbing short-wavelength light and suppressing oxidative stress. To evaluate whether MP levels are related to retinal neural protection and resulting health, we analyzed the association between the MP optical density (MPOD), and the macular thickness and volumes. Forty-three eyes of 43 healthy adult volunteers (21 men and 22 women; age: 22–48 (average 31.4 ± 1.1) years) were analyzed. Highly myopic eyes (<-6 diopters) were excluded. MPOD was measured using MPS2®, and the neural retinal thickness and volume were measured using optical coherence tomography. The mean MPOD was 0.589 ± 0.024, and it positively correlated with the central retinal thickness (P = 0.017, R = 0.360) and retinal volume of the fovea (1-mm diameter around the fovea; P = 0.029, R = 0.332), parafovea (1–3-mm diameter; P = 0.002, R = 0.458), and macula (6-mm diameter; P = 0.003, R = 0.447). In the macular area (diameter: 6 mm), MPOD was correlated with the retinal neural volume of the ganglion cell layer (P = 0.037, R = 0.320), inner plexiform layer (P = 0.029, R = 0.333), and outer nuclear layer (P = 0.020, R = 0.353). Thus, MPOD may help in estimating neural health. Further studies should determine the impact of MP levels on neuroprotection.

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer.

The macular pigment reflectometer (MPR) objectively measures the overall macular pigment optical density (MPOD) and further provides the lutein optical density (L-OD) and zeaxanthin optical density (Z-OD) in the central 1 degree of the fovea. A modification of the technique was developed to evaluate in vivo carotenoid density eccentric to the fovea. An adjustable track system with red LED lights was placed 6.1 m away from the participant to facilitate ocular fixation. Lights were spaced appropriately to create increments of 1 degree retinal disparity during the reflectometry measurements. All reflectometry measurements were obtained with pupillary dilation. The mean MPR-MPOD value for the central measurement was 0.593 (SD 0.161) with an L-OD to Z-OD ratio of 1:2.61. The MPR-MPOD value at 1 degree was 0.248 and the mean MPR-MPOD value at 2 degrees in the parafoveal region was 0.143. The L-OD to Z-OD ratio at 1 degree and 2 degrees off center was 1.38:1.0 and 2.08:1.0, respectively. The results demonstrate that MPOD measurements obtained using the MPR decrease as a function of retinal eccentricity and that there is a higher concentration of zeaxanthin centrally compared to lutein. The L-OD to Z-OD ratio changes with foveal eccentricity, with twice more lutein than zeaxanthin at 2 degrees off center. Our technique successfully provides a quick in vivo method for the measurement of macular pigment optical density at various foveal eccentricities. The results agree with prior published in vivo and in vitro xanthophyll carotenoid density distribution measurements.

Spatial distribution of macular pigment estimated by autofluorescence imaging in elderly Japanese individuals

To determine the spatial distribution types of macular pigment (MP) in elderly Japanese individuals and to consider their origin. Observational case series. Local MP optical density (MPOD) at some eccentricities and MP volume were measured using the MPOD module of a MultiColor Spectralis in 96 pseudophakic eyes of 96 participants (age range, 52-86years; mean age, 72.8 ± 8.3years). The MP distribution types were determined from the MP spatial profiles. The retinal thickness (RT) at the foveal center, at both 0.5° and 0.9° eccentricities, and the foveal width were measured using optical coherence tomography. The mean local MPOD at the foveal center was 0.79. Spatial distribution was classified into four types: central peak (24.0%), ring-like (40.6%), intermediate (22.9%), and central dip (12.5%). The ring-like type was the most frequent in these Japanese participants. The central-peak type showed lower MPOD than did the other types in the area outside 0.9°. The ring-like type occurred frequently in eyes with small RT at 0.5° and wider foveal width. A rough contour of the Müller cell cone was found more frequently in the central-dip type than in the other types. The present characteristics of the different distribution patterns could be explained by the hypothesis that MP presents mainly in the Müller cell cone within 0.5° and in Müller cells in the outer and inner plexiform layers in the area outside 0.5°. The anatomic characteristics of Müller cells at the fovea and parafovea likely affect the MP distribution.

Macular Pigment Optical Density in the Korean Population: a Cross Sectional Study.

BackgroundTo evaluate the macular pigment optical density (MPOD) with age in the Korean population using the Macular Pigment Screener II (MPSII®).MethodsOne hundred and twenty-six eyes were retrospectively reviewed. MPOD was measured using MPSII®, which uses a heterochromatic flicker photometry method, and the estimated values were analyzed. Spearman's correlation test was used to evaluate correlations between MPOD and age. The association between MPOD and age was determined using a simple linear regression analysis. MPODs among the four groups were compared via the post hoc analysis with Bonferroni correction, MPODs between the age-related macular degeneration (AMD) group and aged-matched healthy subjects were compared via the Mann-Whitney U test. Other risk factors for AMD were identified via a logistic regression analysis.ResultsEstimated MPOD decreased significantly with increasing age in the general population. In the simple regression analysis, a statistically significant linear regression model was observed, and the estimated values of MPOD decreased by −0.005 as age increased by 1 year. Aged (> 50 years) showed lower MPOD than younger (30–49 years) subjects. But, in the healthy population, the estimated MPOD values exhibited a decreasing trend with age, but there were no significant differences according to age, after excluding patients with AMD. MPOD was significantly lower in patients with AMD than in aged healthy controls. Furthermore, hypertension, dyslipidemia, and smoking were identified as risk factors for AMD.ConclusionMPOD measured with MPSII® reflects the MP density in healthy individuals and patients with dry AMD. Aging was not significantly associated with low MPOD in healthy population, but the presence of dry AMD was significantly associated with low MPOD. Then, low MPOD may be a risk factor for development of dry AMD. Furthermore, routine screening with MPS II® for ages 50 and older is thought to help detect early low MPOD and identify individuals who should take supplements.

Differences in macular pigment optical density across four ethnicities: a comparative study.

Objective:The aim of this study is to compare macular pigment optical density levels across four different ethnicities and study its influence on ganglion cell layer and retinal nerve fibre layer thickness across these ethnicities.Methods:Consenting adults visiting the ophthalmology and optometry clinics for a routine eye examination without any ocular comorbidity were enrolled. Participants underwent optical coherence tomography for macular thickness, retinal nerve fibre layer thickness and ganglion cell layer thickness. The macular pigment optical density levels were determined in the dominant eye using the QuantifEye device by trained observers.Results:In total, 336 eyes of 336 participants with a mean age of 39.2 ± 14.4 years were included of which 103 (30%) were Caucasians, 111 (33%) were African Americans, 29 (9%) were South Asian Indians and 94 (28%) were Hispanics. The mean macular pigment optical density value across the entire study population was 0.47 ± 0.15. South Asian Indians (0.58 ± 0.16) and Hispanics (0.52 ± 0.15) had significantly higher mean macular pigment optical density values compared with Caucasians (0.41 ± 0.16) and African Americans (0.38 ± 0.15). Linear regression analysis showed that there was a significant association between ethnicities and macular pigment optical density values when adjusted for age (β coefficient = 0.31, 95% confidence interval = 0.029–0.58, p < 0.001 for South Asian Indian and Hispanic ethnic groups compared with African Americans). There were no differences in the retinal nerve fibre layer and ganglion cell layer thickness across ethnic groups. Linear regression analysis also did not reveal any significant association between macular pigment optical density levels and retinal nerve fibre layer or ganglion cell layer thickness.Conclusion:Caucasians and African Americans have lower macular pigment optical density compared with South Asian Indians and Hispanics. There is no clinically significant association between macular pigment optical density levels and retinal nerve fibre layer and ganglion cell layer thickness in healthy individuals across races.

Practical application of ophthalmic nutraceuticals in patients with dry form of age-related macular degeneration

To conduct a placebo-controlled clinical monitoring of visual functions in patients with dry AMD while taking a drug Complex that corresponds to the AREDS 2 formula. The study included 90 people in 3 equivalent parallel groups, two of which were represented by patients with AMD who took the Complex for 3-6 months or Placebo for 3 months. The third group included healthy subjects of the same age. In addition to standard eye examination, participants underwent evaluation of the macular pigment optical density (MPOD), spatial-frequency contrast sensitivity (CS), photostress test, and reading parameters on the Salzburg Reading Desk device. The changes of blood plasma antioxidant activity (AOA) were also evaluated in the main group. By day 90 of the study, patients of the main group showed statistically significant (p<0.001) growth of MPOD from 0.24±0.13 to 0.32±0.1, reduction of photostress recovery time from 68.2±17.8 to 57.3±17.5 seconds, improvement of high-spatial-frequency CS - from 25.0±8.2 to 30.6±6.8 dB, increase of near visual acuity logMAR from 0.22±0.04 to 0.19±0.03 and reading speed, and decrease of reading mistakes. Statistically significant (p<0.05) reduction of in AOA was seen during the observation period from 1.52±0.16 to 1.68±0.16. Patients of the main group who received the Complex for 180 days demonstrated additional improvements of visual functions. The drug Complex corresponding to the AREDS 2 formula contributes to a significant increase in MPOD, improvements of visual functions and overall antioxidant status of patients, which confirms the feasibility of its use in patients with dry AMD.