Macropus Giganteus Research Articles

Multiplex PCRs for the specific identification of marsupial and deer species from faecal samples as a basis for non-invasive epidemiological studies of parasites

BackgroundThe specific identification of animals through the analysis of faecal DNA is important in many areas of scientific endeavour, particularly in the field of parasitology.MethodsHere, we designed and assessed two multiplex PCR assays using genetic markers in a mitochondrial cytochrome b (cytb) gene region for the unequivocal identification and discrimination of animal species based on the specific amplification of DNA from faecal samples collected from water catchment areas in Victoria, Australia. One of these assays differentiates three marsupial species (eastern grey kangaroo, swamp wallaby and common wombat) and the other distinguishes three deer species (fallow, red and sambar deer). We tested these two assays using a total of 669 faecal samples, collected as part of an ongoing programme to monitor parasites and microorganisms in these animals.ResultsThese two PCR assays are entirely specific for these animal species and achieve analytical sensitivities of 0.1–1.0 picogram (pg). We tested 669 faecal samples and found that some previous inferences of species based on faecal morphology were erroneous. We were able to molecularly authenticate all of the 669 samples.ConclusionsWe have established PCR assays that accurately distinguish the faecal samples of some of the prominent large mammalian herbivores found within a water catchment system in the state of Victoria, Australia. The multiplex assays for marsupials and deer produce amplicons that are easily differentiable based on their size on an agarose gel, and can be readily sequenced for definitive species authentication. Although established for marsupials and deer, the methodology used here can be applied to other host-parasite study systems to ensure data integrity.

Predators, fire or resources: What drives the distribution of herbivores in fragmented mesic forests?

AbstractTrophic interactions and disturbance events can shape the structure and function of ecosystems. However, the effects of drivers such as predation, fire and climatic variables on species distributions are rarely considered concurrently. We used a replicated landscape‐scale predator management experiment to compare the effects of red foxVulpes vulpescontrol, time‐since‐fire, vegetation type and other environmental variables on native herbivore distributions. Occurrence data for four native herbivores and an invasive predator – the red fox – were collected from 240 sites across three baited (for lethal fox control) and three unbaited forest blocks (4659–9750 ha) in south‐western Victoria, Australia, and used to build species distribution models. The herbivore taxa were as follows: red‐necked wallabyMacropus rufogriseus, black wallabyWallabia bicolour, grey kangarooMacropus fuligenosusandMacropus giganteusand common brushtail possumTrichosurus vulpecula. Fox control and fire had little effect on herbivore occurrence, despite the literature suggesting it can influence abundance, while climate, proximity to farmland and topography were more influential. This may be because the region’s high productivity and agricultural pastures subsidise food resources for both predators and prey within the forest blocks and so dampen trophic interactions. Alternatively, these drivers may affect herbivore abundance, but not herbivore occurrence. Understanding the drivers of herbivore distributions is an important step in predicting the effects of herbivory on other species, particularly after management interventions such as predator control and prescribed burns.

Not all urban landscapes are the same: interactions between urban land use and stress in a large herbivorous mammal.

Urbanization significantly impacts the health and viability of wildlife populations yet it is not well understood how urban landscapes differ from non-urban landscapes with regard to their effects on wildlife. This study investigated the physiological response of eastern grey kangaroos (Macropus giganteus) to land use at a landscape scale. Using fecal glucocorticoid metabolites (FGM) we compared stress levels of kangaroo populations in urban and non-urban environments. We modeled FGM concentrations from 24 kangaroo populations against land use (urban or non-urban) and other anthropogenic and environmental factors, using a linear modeling approach. We found that land use was a significant predictor of FGM concentrations in eastern grey kangaroos with significant differences in concentrations between urban and non-urban populations. However, the direction of the relationship differed between northern and southern regions of Australia. In the northern study sites, kangaroos in urban areas had significantly higher FGM levels than their non-urban counterparts. In contrast, in southern sites, where kangaroos occur in high densities in many urban areas, urban kangaroos had lower FGM concentrations than non-urban kangaroos. Rainfall and temperature were also significant predictors of FGM and the direction of the relationship was consistent across both regions. These results are consistent with the contrasting abundance and persistence of kangaroo populations within the urban matrix between the two study regions. In the northern region many populations have declined over the last two decades and are fragmented, also occurring at lower densities than in southern sites. Our study indicates that it is the characteristics of urban environments, rather than the urban environment per se, which determines the extent of impacts of urbanization on kangaroos. This research provides insights into how the design of urban landscapes can influence large mammal populations.

Habitat selection in a peri-urban area by a large mammal indicates a low potential for human–wildlife conflict

Abstract ContextIn Australia, various species of macropods (family Macropodidae) are known to occur within peri-urban areas, where they can be a source of human–wildlife conflict. Some species, such as the eastern grey kangaroo (Macropus giganteus), have received considerable research attention over the past few years following demands from land managers for evidence-based management guidelines; however, the ecology of other macropod species found in peri-urban areas, such as the eastern wallaroo (Osphranter robustus robustus), remains poorly understood. AimsThe aims were to determine the home range of male and female eastern wallaroos and assess habitat selection in order to define whether wallaroos in a peri-urban environment should be viewed as thriving (‘matrix-occupying’), persisting (‘matrix-sensitive’) or struggling (‘urban-sensitive’). MethodsHome range and habitat use of six adult male, five adult female and one subadult male eastern wallaroo were investigated using GPS telemetry between October 2017 and May 2018 in the south-west of Sydney. Key resultsHome ranges (mean±s.e.) of males (63.1±10.2ha) were significantly larger than those of females (31.1±3.3ha). Every adult wallaroo had highly overlapping monthly home ranges, indicating strong site fidelity in all individuals. Eastern wallaroos selected habitats based on vegetation composition during the night and canopy cover during the day. Grassland and open native woodlands were preferred during foraging activities at night. By contrast, human-modified habitats, including hard surfaces and lawns, were avoided at all times by all individuals. ConclusionThe results indicate that eastern wallaroos avoid human-modified features in the landscape, so they could be viewed as persisting (‘matrix-sensitive’) in peri-urban areas. ImplicationsCompared with matrix-occupying macropods, such as the eastern grey kangaroo, the eastern wallaroo is less likely to cause human–wildlife conflicts – a result of its avoidance of human-modified habitat. Land-use planning, involving green corridors linking remnant vegetation, should be implemented as part of urban planning to enable the persistence of diverse mammal populations in urban areas, particularly matrix-sensitive species.

Acute Death of Kangaroos in a Zoo Due to Highly Pathogenic Corynebacterium pseudotuberculosis and Yersinia pseudotuberculosis

Background: “Lumpy jaw” is disease effecting wallabies and kangaroos, particularly in Macropus rufus and Macropus giganteus. In the most serious situations, additional tooth loss and fistulas follow, accompanied by a stench, weight loss, and eventually death due to sepsis or blood poisoning. “Lumpy jaw” disease has seriously affected the normal display and health of kangaroos, and cause a huge economic loss. There was an outbreak of jaw infection in kangaroos at the Nanjing Forest Zoo. Two Macropus giganteus and two Macropus rufus died of “lumpy jaw”. The main objective of the describing case was to isolate pathogens, provide a basis for follow-up treatment, and serve to establish a disease prevention protocol.Case: Four grown-up kangaroos (two Macropus giganteus and two Macropus rufus) were raised in Nanjing Forest Zoo, who had obviously clinical symptoms, such as oral lesions of pus, necrotic tissue, rotting teeth, then died of “lumpy jaw”. Oral swab samples were collected from the lesion sites of the dying kangaroos. Mice experiments were conducted to examine the pathogenicity of the strains. Tests of antimicrobial susceptibity were performed to prescribe with better drug treatments for kangaroos. Corynebacterium pseudotuberculosis and Yersinia pseudotuberculosis were identified based on morphology, culture characteristics and biochemical tests. Corynebacterium pseudotuberculosis (G+) in Sucrose, Mannitol, Lactose, Maltose, Glucose tubes were positive, that acids and gases both production, in Gelatin liquefaction, Indol test, MR were positive, that only acids production, others were negative; Yersinia pseudotuberculosis (G-) in Urea, MR were positive, that only acids production, others were negative.The infected mice presented with gum erosion or ulcers when the two pathogens were injected subcutaneous at the oral regional by 2-3 point at 0.2 mL of individual strains（1.0×109 CFU/mouse）. Drug sensitivity tests showed that Corynebacterium pseudotuberculosis is highly sensitive to Erythromycin (E) and Sulfamethoxazole (SMZ); meanwhile, Yersinia pseudotuberculosis is highly sensitive to Sulfamethoxazole (SMZ), Nitrofurantoin (N), Penicillin-G (P-G) and Erythromycin (E). The other “lumpy jaw” kangaroos in the zoo now receive oral screening with antibiotics (SMZ, E).Discussion: Yersinia pseudotuberculosis is one of three pathogenic bacteria of the gram-negative genus Yersinia; it can be found in birdsand other mammals. It also spreads through soil, plants, and insects in the environment.Yersinia pseudotuberculosis can also lead to fatal systemic symptoms in human. Corynebacterium pseudotuberculosis belongs to the Actinobacteria family, which are associated with Caseous lymphadenitis in breeding animals, especially in goats and sheep. The isolates affected the lymph nodes and visceral organs of the kangaroos in this case, who presented with gingivitis or stomatitis. It is the first report of Corynebacterium pseudotuberculosis and Yersinia pseudotuberculosis co-infecting kangaroo and causing a fatal case of “lumpy jaw” in China. In this case, isolation and identification of pathogenic bacteria were carried out on the sick kangaroos, and animal test and drug susceptibity test were conducted. The study results could provide theoretical basis for the follow-up treatment and prevention method of this disease.

Sequence analyses at mitochondrial and nuclear loci reveal a novel Theileria sp. and aid in the phylogenetic resolution of piroplasms from Australian marsupials and ticks.

The order Piroplasmida encompasses two main families: Babesiidae and Theileriidae, containing tick-borne pathogens of veterinary and medical importance worldwide. While only three genera (Babesia, Cytauxzoon and Theileria) comprising piroplasm parasites are currently recognised, phylogenetic studies at the 18S rRNA (18S) gene suggest that these organisms represent at least ten lineages, one of which comprises the relatively unique and highly diverse Theileria spp. from Australian marsupials and ticks. As an alternative to analysing 18S sequences alone, sequencing of mitochondrial genes has proven to be useful for the elucidation of evolutionary relationships amongst some groups of piroplasms. This research aimed to characterise piroplasms from Australian native mammals and ticks using multiple genetic markers (18S, cytochrome c, oxidase subunit III (cox3) and cytochrome B (cytB)) and microscopy. For this, nearly complete piroplasm-18S sequences were obtained from 32 animals belonging to six marsupial species: eastern bettong (Bettongia gaimardi), eastern quoll (Dasyurus viverrinus), eastern grey kangaroo (Macropus giganteus), swamp wallaby (Wallabia bicolor), quokka (Setonix brachyurus) and Gilbert’s potoroo (Potorous gilbertii). The organisms detected represented eight novel Theileria genotypes, which formed five sub-clades within the main marsupial clade containing previously reported Australian marsupial and tick-derived Theileria spp. A selection of both novel and previously described Australian piroplasms at the 18S were also successfully characterised, for the first time, at the cox3 and cytB loci, and corroborated the position of Australian native theilerias in a separate, well-supported clade. Analyses of the cox3 and cytB genes also aided in the taxonomic resolution within the clade of Australian Piroplasmida. Importantly, microscopy and molecular analysis at multiple loci led to the discovery of a unique piroplasm species that clustered with the Australian marsupial theilerias, for which we propose the name Theileria lupei n. sp.

What influences road mortality rates of eastern grey kangaroos in a semi-rural area?

BackgroundRoads have major ecological impacts on wildlife. Vehicle collisions most frequently impact large herbivores due to their larger home range compared to smaller animals, and higher population density compared to carnivores. Kangaroos (Macropus spp.) account for a large proportion of reported wildlife vehicle collisions that occur in N S W, Australia. We aimed to evaluate what influenced road mortality of eastern grey kangaroos (Macropus giganteus) in a temperate rural/suburban region. The location of roadkilled kangaroos found on or near two 1 km stretches of road in Richmond NSW was recorded throughout 2014 and 2015. Weather and moon phase data were recorded for the date of each roadkilled kangaroo. Transects were setup on both roads, and multiple road and landscape features, including the width of roadside, fence construction, habitat type, and distance from street lights measured at 50 m intervals. Data were analyzed to explore which landscape features and temporal factors influenced the occurrence of a roadkill hotspot.ResultsMore kangaroo road mortalities occurred during periods of low temperature and low rainfall, and these factors are likely to affect forage quality. Fewer mortalities occurred when rain was falling. A greater number of mortalities occurred during the waning gibbous phase of the lunar cycle. Significantly more road mortalities occurred a short distance from the end of a section of street lights.ConclusionsThe findings suggest that illumination influences the likelihood of kangaroo road mortalities. Large herbivores are particularly sensitive to habitat fragmentation because they need unrestricted access to large continuous habitat. Knowledge of factors that influence where and when kangaroos are most likely to cross roads can be used to inform more targeted management strategies and improve future road design and habitat connectivity to reduce the incidence of wildlife vehicle collisions.

Flight responses of eastern gray kangaroos to benign or harmful human behavior.

Globally, wilderness is being converted for rural and agricultural land use. In countryside landscapes, many habitat structures remain intact, providing suitable habitat for wildlife species that can accurately assess novel risks and develop tolerance to benign disturbances. Associative learning that promotes avoidance and also facilitates desensitization to benign disturbance is key to persisting in these landscapes. Conversely, learning to distinguish and avoid negative interactions with humans, like hunting, is vital. To determine if eastern gray kangaroos are capable of learning from previous interactions with humans, we tested the flight responses of wild kangaroos which have previously experienced either low or high frequencies of harmful and benign encounters with humans. We found that eastern gray kangaroos rapidly habituated to benign disturbance as there was no significant difference in assessment distance between groups that previously experienced low or high frequencies of disturbance. The threat of harmful disturbances was not as quickly learnt, as groups that experienced low frequencies of harmful disturbance delayed flight longer than those experiencing frequent harm. We found that the influence of environmental and group parameters on a kangaroo's decision to flee depended on the intent and frequency of previous interactions with humans. Our study indicates that kangaroos are learning from previous encounters with humans, correctly assessing novel risks which may be contributing to their persistence in countryside landscapes.

The large-scale capture of eastern grey kangaroos (Macropus giganteus) and red kangaroos (Osphranter rufus) and its application to a population management project.

A large-scale capture method was developed to enable sterilisation of a macropod population in western Sydney from 2005 to 2018. Until March 2007, free ranging eastern grey kangaroos and red kangaroos were herded into purpose-built 15 m diameter capture yards (CYs) for darting with a projectile syringe. From March 2007 onwards, animals were free-range darted in large areas without herding. Kangaroos were darted with 1.33-5.10 mg/kg tiletamine/zolazepam and 0.01-0.02 mg/kg medetomidine, ± 0.03 mg/kg acepromazine. Deaths were monitored. Population counts were performed annually. There were 5825 capture events involving 3963 kangaroos. Over 85% of all captures occurred from 2005 to 2008. Of all reported deaths (n = 523), 135 were attributed to ill health. Musculoskeletal injuries incurred during capture were the main project-related cause of death (n = 116). Post capture myopathy was uncommonly diagnosed following capture (n = 19). The herding and capture method enabled a large number of kangaroos to be mobilised and captured with low mortality rates, and the use of CYs resulted in fewer capture-related injuries and deaths than free-range capture. The drug doses and combinations used for darting were safe and effective, and the capture technique was successfully applied to a population management project.

Commensal bacterial sharing does not predict host social associations in kangaroos.

Social network analysis has been postulated as a tool to study potential pathogen transmission in wildlife but is resource-intensive to quantify. Networks based on bacterial genotypes have been proposed as a cost-effective method for estimating social or transmission network based on the assumption that individuals in close contact will share commensal bacteria. However, the use of network analysis to study wild populations requires critical evaluation of the assumptions and parameters these models are founded on. We test (a) whether networks of commensal bacterial sharing are related to hosts' social associations and hence could act as a proxy for estimating transmission networks, (b) how the parameters chosen to define host associations and delineate bacterial genotypes impact inference and (c) whether these relationships change across time. We use stochastic simulations to evaluate how uncertainty in parameter choice affects network structure. We focused on a well-studied population of eastern grey kangaroos (Macropus giganteus), from Sundown National Park, Australia. Using natural markings, each individual was identified and its associations with other kangaroos recorded through direct field observations over 2years to construct social networks. Faecal samples were collected, Escherichia coli was cultured and genotyped using BOX-PCR, and bacterial networks were constructed. Two individuals were connected in the bacterial network if they shared at least one E.coli genotype. We determined the capacity of bacterial networks to predict the observed social network structure in each year. We found little support for a relationship between social association and dyadic commensal bacterial similarity. Thresholds to determine host associations and similarity cut-off values used to define E.coli genotypes had important ramifications for inferring links between individuals. In fact, we found that inferences can show opposite patterns based on the chosen thresholds. Moreover, no similarity in overall bacterial network structure was detected between years. Although empirical disease transmission data are often unavailable in wildlife populations, both bacterial networks and social networks have limitations in representing the mode of transmission of a pathogen. Our results suggest that caution is needed when designing such studies and interpreting results.

Eastern Grey Kangaroo (Macropus giganteus) Vigilance Behaviour Varies between Human-Modified and Natural Environments.

Simple SummaryUrban landscapes are increasing across the globe, causing wildlife to face new challenges and driving behavioural change. Wildlife in these urban landscapes must adapt their behaviour to survive. We investigated vigilance behaviour in urban and non-urban populations of eastern grey kangaroos. We found that the difference in land use alone did not affect their vigilance behaviour, but kangaroos spent more time vigilant in areas of high human population density. Season and sex also influenced the amount of time spent vigilant: more time was spent vigilant in winter and in female kangaroos. This is the first study to compare the vigilance behaviour between urban and non-urban populations of a large mammal across regions, giving the first insight into how kangaroos adapt their behaviour in urban environments.Rapid increases in urban land use extent across the globe are creating challenges for many wildlife species. Urban landscapes present a novel environment for many species, yet our understanding of wildlife behavioural adaptations to urban environments is still poor. This study compared the vigilance behaviour of a large mammal in response to urbanisation at a landscape level. Here, we investigate urban (n = 12) and non-urban (n = 12) populations of kangaroos in two regions of Australia, and the relationship between kangaroo vigilance and urbanisation. We used a linear modelling approach to determine whether anti-predator vigilance and the number of vigilant acts performed were influenced by land use type (i.e., urban or non-urban), human population densities, kangaroo demographics, and environmental factors. Kangaroo behaviour differed between the two study regions; kangaroo vigilance was higher in urban than non-urban sites in the southern region, which also had the highest human population densities, however no effect of land use was found in the northern region. Season and sex influenced the vigilance levels across both regions, with higher levels seen in winter and female kangaroos. This study is the first to compare urban and non-urban vigilance of large mammals at a landscape level and provide novel insights into behavioural adaptations of large mammals to urban environments.

Towards a Dynamic Model of the Kangaroo Knee for Clinical Insights into Human Knee Pathology and Treatment: Establishing a Static Biomechanical Profile

There is limited understanding of how patella realignment or patellectomy to surgically manage patellofemoral pain (PFP) affects knee biomechanics. By analysing marsupials like kangaroos that lack an ossified patella, actionable biomimetic insight for the management of end-stage PFP could be gained. This study aimed to provide the foundation of a multi-stage approach, by establishing a static biomechanical profile of the kangaroo stifle that informs the inputs and factors requiring consideration for future dynamic analyses. Volumetric CT and MRI sequences were obtained for four hindlimbs from two Macropus giganteus specimens, from which three-dimensional models of the stifles were created. Two limbs were dissected to visualise the insertion points, origins and lines of action of the quadriceps muscles and the knee extensor mechanism. Static measurements were obtained from the three-dimensional models to establish the biomechanical profile. The results confirmed structural differences in the kangaroo stifle with lack of an ossified patella, a prominent tuberosity and a shorter femur, which functionally affect the mechanical advantage and the torque-generating capability of the joint. The data reported in this study can be used to inform the inputs and constraints of future comparative analyses from which important lessons can be learned for the human knee.

Morphological characteristics of the kangaroo lumbar intervertebral discs and comparison with other animal models used in spine research.

Animal models are frequently used to elucidate pathomechanism and pathophysiology of various disorders of the human intervertebral disc (IVD) and also to develop therapeutic approaches. Here we report morphological characteristics of the kangaroo lumbar IVDs and compare them with other animal models used in spine research. Twenty-five fresh-frozen cadaveric lumbar spines (T12-S1) derived from kangaroo carcases (Macropus giganteus) of undetermined age were first scanned in a C-Arm X-ray machine. A photograph of the axial section of the disc including a calibrated metric scale was also acquired. The digital radiographs and photographs were processed in ImageJ to determine the axial and sagittal plane dimensions for the whole disc (WD) and the nucleus pulposus (NP) and the mid-sagittal disc height for all the lumbar levels. Our results suggest that the L6-S1 IVD in kangaroos is distinctly large compared with the upper lumbar IVDs. Based on previously published data, human lumbar IVDs are the largest of all the animal IVDs used in spine research, with camelid cervical IVDs being the closest relative in absolute dimensions (llamas: 78% in disc height, 40% in WD volume, and 38% in NP volume). Kangaroo L6-S1 IVD was approximately 51% in height, 20% in WD volume, and 20% in NP volume of the human lumbar IVD. We conclude that morphological similarities exist between a kangaroo and human lumbar IVD, especially with the lima bean shape in the axial plane, wedge shape in the sagittal plane, convexity at the cephalad endplates, and percentage volume occupied by the NP in the IVD. These slides can be retrieved under Electronic Supplementary Material.

Behavioural Plasticity by Eastern Grey Kangaroos inResponse to Human Behaviour.

Simple SummaryMany species of wildlife live in landscapes they share with people. Some exploit resources and protection provided by close proximity to people, while others learn to avoid people all together. In this study, we sought to test whether individuals from a population of eastern grey kangaroos altered grouping and spacing behaviour in response to human presence, depending upon whether the intent and actions of those people were benign or harmful. Under harmful conditions, kangaroos failed to form larger groups when far from cover, however, this typical antipredator grouping behaviour persisted when human disturbances were benign. These differences in grouping and spacing behaviour suggest that kangaroos can exhibit bidirectional behavioural plasticity at fine scales, a trait that may confer adaptive advantages when sharing landscapes with humans.Sharing landscapes with humans is an increasingly fraught challenge for wildlife across the globe. While some species benefit from humans by exploiting novel opportunities (e.g., provision of resources or removal of competitors or predators), many wildlife experience harmful effects, either directly through persecution or indirectly through loss of habitat. Consequently, some species have been shown to be attracted to human presence while others avoid us. For any given population of a single species, though, the question of whether they can recognise and change their response to human presence depending on the type of human actions (i.e., either positive or negative) has received little attention to date. In this study, we chose to examine the behavioural plasticity within a single population of eastern grey kangaroos (Macropus giganteus) to both positive and negative human activity. Within a relatively small and contiguous landscape, we identified areas where kangaroos experience a combination of either low and high frequencies of benign and harmful human disturbances. From six sampling sessions over five months, we found that density and group sizes were higher where humans acted benignly towards them, and that these groups had higher representations of sub-adults and juveniles than where humans had harmful intentions. Importantly, we found that the vital antipredator strategy of increasing group size with distance from cover was not detectable at sites with low and high levels of harm. Our findings suggest that these kangaroos are recognising and adjusting their behavioural response to humans at fine spatial scales, a plasticity trait that may be key to the survival of these species in human dominated landscapes.

Fright or Flight? Behavioural Responses of Kangaroos to Drone-Based Monitoring

Drones are often considered an unobtrusive method of monitoring terrestrial wildlife; however research into whether drones disturb wildlife is in its early stages. This research investigated the potential impacts of drone monitoring on a large terrestrial mammal, the eastern grey kangaroo (Macropus giganteus), in urban and peri-urban environments. We assessed the response of kangaroos to drone monitoring by analysing kangaroo behaviour prior to and during drone deployments using a linear modelling approach. We also explored factors that influenced kangaroo responses including drone altitude, site characteristics and kangaroo population dynamics and demographics. We showed that drones elicit a vigilance response, but that kangaroos rarely fled from the drone. However, kangaroos were most likely to flee from a drone flown at an altitude of 30 m. This study suggests that drone altitude is a key consideration for minimising disturbance of large terrestrial mammals and that drone flights at an altitude of 60–100 m above ground level will minimise behavioural impacts. It also highlights the need for more research to assess the level of intrusion and other impacts that drone surveys have on the behaviour of wildlife and the accuracy of the data produced.

Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus).

Marsupials are highly susceptible to Toxoplasma gondii infection. Here, we report T. gondii infection in four kangaroos from a zoo in China. Kangaroos were imported into China in 2000 and were since bred in zoo. In 2017–2018, four kangaroos died due to respiratory system disease or injury. The bodies were submitted to the laboratory to test for T. gondii infection. Antibodies to T. gondii were found in 75% (3/4) of the kangaroos via the modified agglutination test with the cut-off 1:25. Cysts were observed in the histopathological sections of tongue and diaphragm or squashes of fresh myocardium in two kangaroos. These cysts were confirmed as T. gondii by immunohistochemical staining and molecular biological analysis. One viable T. gondii strain was isolated from one kangaroo and designated as TgRooCHn1. DNA from T. gondii tachyzoites obtained from cell culture was characterized by 10 PCR-RFLP markers and the virulence genes ROP5 and ROP18. The genotype of this isolate did not match with any known genotypes; it was designated as ToxoDB#292. The virulence of TgRooCHn1 (104 tachyzoites) was non-lethal to mice, and it formed tissue cysts. To our knowledge, the present study is the first isolation of ToxoDB#292 strain from kangaroo. Improvemets for captive settings were initiated, including greater attention being paied to birds and stray cats, fed frozen meat for carnivores.

In vitro binding of cefovecin to plasma proteins in Australian marsupials and plasma concentrations of cefovecin following single subcutaneous administration to koalas (Phascolarctos cinereus).

Cefovecin has a long duration of antibiotic activity in cats and dogs, somewhat attributable to its high plasma protein binding. To determine the cefovecin binding to plasma proteins in vitro in selected Australian marsupials and to quantify the change in cetovecin concentration over time following subcutaneous injection in koalas. Various cefovecin concentrations were incubated with plasma and quantified using HPLC. The median (range) bound percentages when 10 μg/mL of cefovecin was incubated with plasma were 11.1 (4.1-20.4) in the plasma of the Tasmanian devil, 12.7 (5.8-17.3) in the koala, 18.9 (14.6-38.0) in the eastern grey kangaroo, 16.9 (15.7-30.2) in the common brush-tailed possum, 37.6 (25.3-42.3) in the eastern ring-tailed possum and 36.4 (35.0-38.3) in the red kangaroo, suggesting that cefovecin may have a shorter duration of action in these species than in cats and dogs. Cefovecin binding to plasma proteins in thawed, frozen equine plasma was also undertaken for assay quality control and the median (range) plasma protein binding (at 10 μg/mL) was 95.6% (94.9-96.6%). Cefovecin was also administered to six koalas at 8 mg/kg subcutaneously and serial blood samples were collected at 3, 6, 24, 48, 72, 96 h thereafter. Cefovecin plasma concentrations were not quantifiable in four koalas and in the other two, the mean plasma concentration at t = 3 h was 1.04 ± 0.01 μg/mL. Because of the limited pharmacokinetic data generated, no further pharmacokinetic analysis was performed; however, a single injected bolus of cefovecin is likely to have a short duration of action in koalas (hours, rather than days).

Shifting faunal baselines through the Quaternary revealed by cave fossils of eastern Australia.

Fossils from caves in the Manning Karst Region, New South Wales, Australia have long been known, but until now have never been assessed for their palaeontological significance. Here, we report on late Quaternary faunal records from eight caves in the region. Extinct Pleistocene megafaunal taxa are recognised in two systems and include giant echidnas (Tachyglossidae gen. et sp. indet.), devils (Sarcophilus laniarius), koalas (Phascolarctos stirtoni), marsupial ‘lions’ (Thylacoleo carnifex), and kangaroos (Macropus giganteus titan). Some caves contain skeletal remains of introduced exotics such as sheep and dogs, but also provide a rich record of small-bodied native species including Eastern Bettongs (Bettongia gaimardi), Eastern Chestnut Mice (Pseudomys gracilicaudatus), and White-footed Rabbit Rats (Conilurus albipes). These endemics are either locally extirpated or have suffered total extinction in the historic period. Their skeletal and dental remains were recorded as unmineralised surface specimens in the caves, indicating that they are recent in age. Extant populations have never been recorded locally, thus, their probable loss from the region in historic times had gone unnoticed in the absence of palaeo-evidence. Our findings suggest that the supposed habitat tolerances of such species have been substantially underestimated. It is highly likely that modern populations have suffered niche contraction since the time of European colonisation of the continent. The local extirpations of several species of digging mammal has likely led to decreased functionality of the current ecosystem.

Macropods, feral goats, sheep and cattle. 2. Equivalency in what and where they eat

The extent to which sheep, cattle, feral goats, red kangaroos, western grey kangaroos, euros and eastern grey kangaroos are equivalent in their use of the Australian southern rangelands is partly dependent on the extent to which their diets and foraging areas overlap. These herbivores all eat large amounts of green annual grasses, ephemeral forbs and the green leaf of perennial grasses when they are available. Overlap in use of these forages by all seven herbivores is concurrent and high. As the abundance of these preferred forages declines, sheep, cattle and feral goats consume increasing amounts of mature perennial grasses and chenopod and non-chenopod perennial forbs. Red kangaroos and western grey kangaroos continue to graze mature perennial grasses longer than sheep, cattle and feral goats, and only switch to perennial forbs when the quantity and quality of perennial grasses are poor. Consequently, overlap in use of perennial forbs by sheep, cattle, feral goats, red kangaroos and western grey kangaroos is sequential and moderately high. When palatable perennial forbs are eaten out, the diets of all herbivores except feral goats comprise predominantly dry perennial grass, and overlap is again concurrent and high. In comparison, feral goats have higher preferences for the browse of a wide range of shrubs and trees, and switch to these much earlier than the other herbivores. When perennial grasses and perennial forbs become scarce, sheep, feral goats and cattle browse large shrubs and trees, and overlap is sequential and high. If climatic conditions remain dry, then red and western grey kangaroos will also browse large shrubs and trees, but overlap between them, sheep, cattle and goats is sequential and low. In contrast to the other herbivores, the diets of euros and eastern grey kangaroos are comprised predominantly of perennial grasses, regardless of climatic conditions. As for diet composition, concurrent overlap in foraging distributions of sheep, cattle, feral goats and the four species of macropods is often low. However, over periods of several months to two or three years, as climatic conditions change, overlap in foraging distributions is sequential and high. While equivalency in what and where these herbivores eat is not quantifiable, it appears to be high overall. This is particularly so for perennial grass, which is the dominant forage for herbivores in the southern rangelands.

The Eastern Grey Kangaroo: A Modern Conservation Dilemma

ABSTRACT The interaction between Eastern Grey Kangaroos (Macropus giganteus) and humans in peri-urban locations is one that excites great passion between conflicting views. Are the kangaroos a prob...