AbstractIdentification of water flow through non‐root soil macropores and along roots using X‐ray computed tomography (CT) is still challenging because of limited methods to quantify roots and pore structures. This study selected shrub‐encroached grassland consisting of a mosaic of shrub patches and interspace grass patches and the original grassland as a control treatment. Twelve intact soil columns (0–30 cm deep) were excavated, which included four replicates each from the soils under the original grassland, the shrub patches and interspace grass patches. Three‐dimensional networks of soil macropores and roots were quantified using CT scanning and image analysis techniques, and active flow pathways were identified through KI tracing experiment using CT scanning. The results showed that the tracer depths of soils under the shrub patches and interspace grass patches were deeper than those of the original grassland, which was attributed to the well‐developed root system. The tracer volume density was significantly and positively correlated with soil macropore node density and root volume density (p < 0.05), and significantly and negatively correlated with root mean angle (p < 0.05). Soils under the shrub patches had lower tracer volume density than that under the interspace grass patches, which was attributed to the horizontal‐oriented roots under the shrub patches. The relative contribution of water flow along roots was larger than that through non‐root soil macropores 35 min after dye application. This provided direct evidence that shrub roots were the main pathway of macropore flow resulting in water flow into deep soil layers in the shrub‐encroached grassland.Highlights The tracer depths of shrub‐encroached grassland were deep due to well‐developed root system. Shrub patches had low tracer volume density due to the horizontal‐oriented roots. The contribution of water flow along roots was larger than that through non‐root soil macropores. Shrub roots were the main pathway of water flow.
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