SummarySoil texture influences the transformation of soil organic matter (SOM), and depletion and reduction in the number of macroaggregates. However, the extent of changes in SOM in response to its quality and mechanisms of stabilization in different types of soil texture is unclear. We evaluated changes in SOM during a 30‐week incubation experiment using seven soils mixed with two sources of litter; Vachellia karroo (Hayne) Banfi & Galasso leaf and Zea mays L. stover. We hypothesized that changes in intraparticulate organic matter (iPOM) are influenced by litter quality • soil type • time interactions, which affect rates of turnover of macroaggregates. A completely randomized design was used in arranging soil jars in the incubator. Free light fractions and coarse and fine iPOM within macroaggregates (>250 μm) decreased exponentially from week 1 to 30 and were significantly (P < 0.05) affected by litter source • soil horizon • time interactions. Trends of changes in fine iPOM fractions were the same in macroaggregates and microaggregates (P < 0.05). The greatest changes in POM fractions occurred 8 weeks after litter incorporation. Both V. karroo leaf and Z. mays stover stabilized macroaggregates during the first 8 weeks after incubation; thereafter they lost their influence. Therefore, we needed to reapply fresh litter after this period. Changes in iPOM fractions and macroaggregate turnover were specific to a soil type, suggesting that different sources of litter have different stabilizing effects in macroaggregates. Soil macroaggregate stabilization and changes in iPOM could be used as indicators to apply more appropriate management practices for soil protection or productivity.HighlightsDoes the source of organic matter affect transformation rates of different intraparticulate organic matter in the soil? Degradation of organic matter into very fine particulate matter also increases the rate of turnover of macroaggregates. Soil type • litter source interactions greatly influenced the rate of transformation from coarse to fine intraparticulate fractions. Effects of litter quality on dynamics of POM fractions in aggregates and macroaggregate turnover were horizon specific.