Rapid wear progression of cutting insert associated with attainment of excessive tool-tip temperature are indispensable causes which limit operational domain of cutting velocity during dry turning of Ti64 alloy. Again, to counteract demerits of flood cooling, jet of air-oil mist (MQL technology) is employed in which water-based coolants or vegetable oils are highly preferable. On the other hand, inclusion of nano-additives within base fluid, and supply the same through MQL system (NFMQL) is also a trendy area of research. Application potential of NFMQL is understood over conventional MQL in terms of better cooling, and lubrication effects due to improved thermo-physical, and tribological properties of the resultant cutting fluid. In this context, present study aims to assess performance of MQL jet containing biodegradable Jatropha oil (carried by pressurized air) when applied during longitudinal turning of Ti64 work alloy. In addition, advantages of 2D layered-structured graphene nanoplatelets (when dispersed into Jatropha oil), in purview of machining performance on difficult-to-cut Ti64 alloy under NFMQL, are studied in this work. Experimental data are compared on the basis of different lubrication conditions (dry, conventional MQL, and NFMQL). Morphology of tool wear is studied in detail. The work extends towards studying chip morphology and machined surface finish of the end product, as influenced by variation in lubrication conditions.