Lymphoma Of Spleen Research Articles

Targeted Deletion of Glycoprotein B Gene by CRISPR/Cas9 Nuclease Inhibits Gallid herpesvirus Type 3 in Dually Infected Marek's Disease Virus-Transformed Lymphoblastoid Cell Line MSB-1.

ABSTRACTMarek’s disease virus (MDV) is a member of the genus Mardivirus in the subfamily Alphaherpesvirinae. There are three different serotypes of MDV designated as MDV-1 (Gallid herpesvirus type 2), MDV-2 (Gallid herpesvirus type 3), and MDV-3 (Meleagrid herpesvirus 1, herpesvirus of turkeys, HVT). MDV-1 is the only serotype that induces Marek’s disease (MD), a lymphoproliferative disorder resulting in aggressive T-cell lymphomas and paralytic symptoms. In the lymphomas and lymphoblastoid cell lines (LCL) derived from them, MDV establishes latent infection with limited viral gene expression. The latent viral genome in LCL can be activated by co-cultivation with chicken embryo fibroblast (CEF) monolayers. MSB-1, one of the first MDV-transformed LCL established from the splenic lymphoma, is distinct in harboring both the oncogenic MDV-1 and non-oncogenic MDV-2 viruses. Following the successful application of CRISPR/Cas9 editing approach for precise knockdown of the MDV-1 genes in LCL, we describe here the targeted deletion of MDV-2 glycoprotein B (gB) in MSB-1 cells. Due to the essential nature of gB for infectivity, the production of MDV-2 plaques on CEF was completely abolished in the MDV-2-gB-deleted MSB-1 cells. Our study has demonstrated that the CRISPR/Cas9 system can be used for targeted inactivation of the co-infecting MDV-2 without affecting the MDV-1 in the MSB-1 cell line. Successful inactivation of MDV-2 demonstrated here also points toward the possibility of using targeted gene editing as an antiviral strategy against pathogenic MDV-1 and other viruses infecting chickens.IMPORTANCE Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by rapid-onset lymphomas in multiple organs and by infiltration into peripheral nerves, causing paralysis. Lymphoblastoid cell lines (LCL) derived from MD lymphomas have served as valuable resources to improve understanding of distinct aspects of virus-host interactions in transformed cells including transformation, latency, and reactivation. MDV-transformed LCL MSB-1, derived from spleen lymphoma induced by the BC-1 strain of MDV, has a unique feature of harboring an additional non-pathogenic MDV-2 strain HPRS-24. By targeted deletion of essential gene glycoprotein B from the MDV-2 genome within the MSB-1 cells, we demonstrated the total inhibition of MDV-2 virus replication on co-cultivated CEF, with no effect on MDV-1 replication. The identified viral genes critical for reactivation/inhibition of viruses will be useful as targets for development of de novo disease resistance in chickens to avian pathogens.

Primitive Splenic Lymphoma: A Case Report and Review of the Literature

Introduction: Malignant lymphomas are defined as malignant proliferations developed from constituents of lymphoid organs, but also from lymphoid formations contained in most organs (liver, kidneys, digestive) without blood contamination. Lymphoma is a set of monoclonal tumor proliferations developed at the expense of lymphocytes. Lymphoma is the most common malignant tumor of the spleen, but primary lymphoma of the spleen remain an exceptional presentation. We report the case of a stercoral perforation of the colon that occurred in a 67-year-old patient whose diagnosis was made on ct and we try to recall the clinical and CT characteristics of primary aneurismal spleen lymphoma. Case Report: We expose the case of a 66-year-old patient, with a history of chronic smoking, consulted in the emergency department for a sub occlusive syndrome evolving for less than 24hours. An abdominal CT was conducted: The diagnosis of stercoral colonic perforation was retained. Due to a development of hypovolemic shock, the decision was to do a laparotomy with peritoneal lavage with colonic resection followed by an ostomy of the proximal segment and drainage of the abdominal cavity. The evolution was characterized by the discharge of the patient 7 days after. Conclusion: Stercoral perforation of the colon is a rare complication of abdominal trauma. The diagnosis, often difficult and delayed, must be known by all physicians caring working in trauma centers. Stercoral perforation of the colon is a surgical emergency, the prognosis of which, often poor, depends on the terrain and the speed of treatment.

The effect of surgery on primary splenic lymphoma: A study based on SEER database.

BackgroundAlthough primary splenic lymphoma (PSL) is rare, it ranks first among splenic primary malignant cancers, and the incidence of lymphoma of spleen has gradually increased in recent years. However, the efficacy of surgery for PSL has not been clinically verified by large sample data, which has affected the formulation of relevant guidelines.AimTo assess whether surgery can enhance the prognosis PSL patients.MethodsExtracted the data of patients with PSL from The Surveillance, Epidemiology, and End Results (SEER) database, and divided the patients into surgery and non‐surgery group. Kaplan–Meier curves and log‐rank tests were used to compare the overall survival (OS) and cancer‐specific survival (CSS). The propensity score matching (PSM) was used to match the data, then compared the OS and CSS again. The COX proportional hazard regression model was used for univariate and multivariate analysis. Finally, we performed subgroup analysis in different Ahmann stages.ResultsA sum of 2207 patients with PSL were enrolled, of which 1062 (48.1%) patients received surgery, and 1145 (51.9%) patients did not undergo surgery. Overall, patients in the surgery group had better OS and CSS. After the propensity scores matching, surgery was not statistically significant in OS and CSS. In the subgroup analysis, surgery was a protective factor for the OS and CSS in Ahmann I/II. However, surgery was no statistical significance in OS and CSS in Ahmann III. In patients with Ahmann Ⅰ/Ⅱ SMZL, surgery was a protective factor for OS and CSS. In patients with Ahmann Ⅲ SMZL, surgery was also statistically significant of OS and CSS.ConclusionsSurgery can significantly improve the prognosis of patients with Ahmann Ⅰ/Ⅱ primary splenic lymphoma, but there was no survival difference in the Ahmann Ⅲ patients with or without surgery. For patients with SMZL, surgery was effective for improving OS and CSS.

Spectrum of rare splenic lesions

Introduction: The human spleen is the largest component of the reticuloendothelial system, having unique anatomic and functional importance. Spleen has been considered as mysterious organ with World population incidence of focal splenic lesions ranging from 0.103 to 0.20%. Aims and Objective: The aim of the present study was to study the pathological spectrum of rare lesions of spleen. Materials and Methods: The present study was conducted in the department of Pathology in a tertiary care centre, over a period of three years from Aug 2014 to July 2017. Results: Total 10 rare cases of splenic lesions were studied. Out of which eight were splenectomies and two were from fetal autopsies. Primary lesions were 9 and secondary lesion was 1. We found one case each of hemangiomatosis of spleen, small B cell lymphoma of spleen, Sickling of spleen, hydatid cyst of spleen, traumatic spleen avulsion, metastasis to spleen from lung and two case were of asplenia with right isomerism and situsambigus and two cases were of extra lymphatic filariasis of spleen. Conclusion: Morphological changes in splenic lesions are less discussed due to fewer number of splenectomy cases. We hereby analyzed rare splenic lesions which has contributed to the knowledge of splenic pathology. Keywords: Pathological spectrum, Splenic lesions, Splenectomy.

B-cell neoplasms and Hodgkin lymphoma in the spleen

B-cell lymphoma of spleen may be primary (most commonly splenic diffuse large B-cell lymphoma) or secondary (typically low-grade non-Hodgkin lymphoma). Depending on the specific lymphoma subtype, there may be a predominantly white pulp pattern of involvement, a predominantly red pulp pattern or a focal nodular pattern. Splenectomy is the ideal specimen for a multiparametric integrative diagnosis of splenic lymphoma, as it allows for a combined study of morphology, immunohistology, flow cytometry, cytogenetics, and molecular genetic techniques. This review article describes the clinicopathologic characteristics of all the relevant B-cell neoplasms that may be encountered in a splenic biopsy or a splenectomy specimen.

Features of immunophenotypic finding B-cell lymphoproliferative diseases by flow cytometry

Aim. To assess the information content of conventional and additional immunophenotypic markers (CD200, CD305) in the differential diagnosis B-cell lymphoproliferative diseases by flow cytometry.
 Methods. An immunophenotypic study using 4-color flow cytometry was performed in 204 patients with different variants of B-cell non-Hodgkin's lymphomas. The study material included peripheral blood and bone marrow. The expression of CD45, CD19, CD20, CD22, CD79b, CD79a, CD5, CD10, CD23, FMC7, CD43, CD38, CD11c, CD103, CD25, CD 200, CD 305, light chains of immunoglobulins (kappa/lambda) using monoclonal antibodies (Becton Dickinson, USA) was evaluated. The intensity of antigen expression was assessed using mean fluorescence intensity (y. e.).
 Results. Conventional FMC7-positive expression revealed only half patients with different variants of leukemization of non-Hodgkin's lymphomas, whereas atypical positive expression of CD23 was observed in patients with marginal spleen lymphoma and follicular lymphoma in 27.3 and 28.6% of cases, respectively. In mantle cell lymphoma, expression of CD200 in B-cell was detected in a significantly smaller number of observations, accompanied by a significant decrease in the average intensity of CD200 fluorescence compared to B-cell chronic lymphocytic leukemia (B-CLL) cells. The mean fluorescence intensity (MFI) of CD305 in hairy cell leukemia is significantly higher than in splenic marginal zone lymphoma (SMZL) with villous lymphocytes.
 Conclusion. Different levels of the information content of some conventional markers were revealed in differential immunophenotypic diagnosis of B-cell lymphoproliferative diseases by flow cytometry; the use of additional markers CD200 and CD305 was highly informative in differential diagnostics between different variants of B-cell lymphoproliferative diseases with similar immunophenotypic and morphological characteristics of lymphoid elements.

Contributions in the Fields of Pathology Beginning With Spleen Lymphoma: A Review

Contributions in the Fields of Pathology Beginning With Spleen Lymphoma: A Review

Lymphoma of the Spleen in a Developing Community

From China, there was the report of a female patient with spleen lymphoma which involved the pancreas. In the USA case, the presentation was by way of rupture. Therefore, 4 Nigerian cases are deemed to be worthy of documentation.

Primary spleen lymphoma visualized with contrast enhanced ultrasound – Case report

Primary spleen lymphoma visualized with contrast enhanced ultrasound – Case report

T-Cell/Histiocyte-Rich B-Cell Lymphoma of Spleen: A Case Report

Splenomegaly is a frequent yet challenging condition that clinicians have to deal within their daily clinical practice. Although the spleen is commonly involved in disseminated lymphoma, primary lymphoma of the spleen is rare. A great majority of the reported cases are of B-cell lineage, with diffuse large B-cell lymphoma being the most common. A new variant of T-cell/histiocyte-rich B-cell was first described by Dogan et al. in 2003.1 The combined morphologic, immunohistochemical, and molecular genetic data are essential for a correct diagnosis of splenic T-cell-rich B-cell lymphoma. We report a classical case of T-cell-rich B-cell lymphoma of the spleen in a 45-year-old male patient with clinical diagnosis of splenomegaly.

Pathologic Splenic Rupture Related to Non-Hodgkin Lymphoma

Introduction: Pathologic splenic rupture (PSR) is described as the spontaneous rupture of a histologically diseased spleen in the absence of trauma. Although it is a complication that often occurs related to infectious diseases, it may rarely be observed in hematologic malignancies. A limited number of case reports about PSR exist in the current literature, even though the spleen is commonly involved in hematologic diseases. Hence, there is a lack of data in the current literature in terms of the clinical symptoms, treatment, and outcomes of the disease. In this study, we aimed to contribute to current data by discussing clinical outcomes of a case with PSR related to spleen lymphoma. Case Report: A 57-year-old woman with a 5-month history of colic left upper abdominal pain was referred to our clinic. In her imaging tests, a splenic mass with a measure of 6x4 cm was detected. The patient underwent splenectomy due to PSR while preparing for elective surgery. Conclusion: PSR should be considered a rare complication in hematologic malignancies. Early diagnosis and treatment are the major factors that determine prognosis. Splenectomy seems to be the most appropriate approach in the treatment of PSR, even though a conservative approach is suggested in some cases.

Inhalation carcinogenicity of 1,1,1-trichloroethane in rats and mice

Carcinogenicity of 1,1,1-trichloroethane (TCE) was examined by an inhalation exposure of F344 rats and BDF1 mice of both sexes to TCE at 0, 200, 800 or 3200 ppm for 6 h/d, 5 d/week for 104 weeks. In male rats, the incidences of bronchiolo-alveolar adenomas and peritoneal mesotheliomas were significantly increased in the 800 and 3200 ppm-exposed groups, respectively. The incidence of bronchiolo-alveolar adenomas in the 3200 ppm-exposed groups exceeded the range of historical control data in the Japan Bioassay Research Center. In female rats, the tumor incidences were not increased in any organs of the TCE-exposed groups. In male mice, a significant positive trend with dose was shown for incidences of bronchiolo-alveolar carcinomas, combined incidences of bronchiolo-alveolar adenomas/carcinomas and hepatocellular adenomas. The incidence of Harderian gland adenomas was significantly increased in the 3200 ppm-exposed group, and malignant lymphomas of spleen at this highest dose exceeded the range of historical control data. In female mice, the combined incidence of bronchiolo-alveolar adenomas/carcinomas was significantly increased in the 3200 ppm-exposed group, and the incidences of hepatocellular adenomas and combined incidences of hepatocellular adenomas/carcinomas were significantly increased in the 200, 800 and 3200 ppm-exposed groups with dose dependence except the combined incidence of hepatocellular adenomas/carcinomas in the 200 ppm-exposed group. The incidences of bronchiolo-alveolar adenomas in the 3200 ppm-exposed group and combined incidences of hepatocellular adenomas/carcinomas in the 200 ppm-exposed groups exceeded the ranges of historical control data. Thus, this study provided clear evidence of inhalation carcinogenicity for TCE in both rats and mice.

Primary diffuse large B cell lymphoma of spleen mimicking splenic abscess – a case report

Primary diffuse large B cell lymphoma of spleen mimicking splenic abscess – a case report

Laparoscopic splenectomy: a single center experience. Unusual cases and expanded inclusion criteria for laparoscopic approach

Laparoscopic splenectomy (LS) is nowadays considered as the gold standard for most hematological diseases where splenectomy is necessary, but many questions still remain. The aim of this study was to analyze our 5-years experiences consisting of 48 consecutive LS cases in order to assess the optimal approach and the feasibility of the procedure also in malignant diseases and unusual cases such as a primary spleen lymphoma, a big splenic artery aneurism, or a spleen infarct due to a huge pancreatic pseudo-cyst. Forty-eight consecutive patients underwent LS from January 2006 to January 2011 with at least 1-year follow-up. Clinical data and immediate outcome were retrospectively recorded; age, diagnosis, operation time, perioperative transfusion requirement, conversion rate, accessory incision, hospital stay, and complications were analyzed. We had 14 cases of malignant splenic disease, the most frequent malignant diagnosis was non-Hodgkin's lymphoma (12/14, 85.7%). Splenomegaly (interpole diameter (ID) >20cm) was observed in 12 cases (25%) and massive splenomegaly (ID >25cm) in 3 cases (6.25%). Conversion to laparotomy occurred in two patients (4.16%), both associated to uncontrollable bleeding in patients with splenomegaly. Mean operative time was 138±22min. Mean hospital stay was 4.5days. Postoperative morbidity rate was 8.8% for the benign group and 35.7% in the malignant group. Mortality occurred in 1/48 patients (2.08%), as a result of overwhelming post-splenectomy infection (OPSI). LS can be performed safely for malignant splenic disease and splenomegaly without any statistically significant increase of morbidity and mortality rate. Conversion rate is increased for massive splenomegaly. LS should be considered as the preferential approach even in patients with malignant disease, splenomegaly, or unusual cases. Massive splenomegaly should be considered as relative contraindication to LS even at experienced centers.

Primary diffuse large B cell lymphoma of spleen mimicking splenic abscess - a case report

Primary splenic lymphoma is defined as generalized lymphoma with splenic involvement as the dominant feature. Primary splenic lymphoma is an infrequent disease. It comprises less than 1% of the all malignant lymphoma. We present a case of 55 years old male with fever, pain and lump in abdomen of 1 month duration. The purpose of this paper is to report a rare occurrence of primary splenic lymphoma and to demonstrate the possibility of it misdiagnosed as splenic abscess clinically and on imaging.

Whole-genome sequencing identifies recurrent somatic NOTCH2 mutations in splenic marginal zone lymphoma

Splenic marginal zone lymphoma (SMZL), the most common primary lymphoma of spleen, is poorly understood at the genetic level. In this study, using whole-genome DNA sequencing (WGS) and confirmation by Sanger sequencing, we observed mutations identified in several genes not previously known to be recurrently altered in SMZL. In particular, we identified recurrent somatic gain-of-function mutations in NOTCH2, a gene encoding a protein required for marginal zone B cell development, in 25 of 99 (∼25%) cases of SMZL and in 1 of 19 (∼5%) cases of nonsplenic MZLs. These mutations clustered near the C-terminal proline/glutamate/serine/threonine (PEST)-rich domain, resulting in protein truncation or, rarely, were nonsynonymous substitutions affecting the extracellular heterodimerization domain (HD). NOTCH2 mutations were not present in other B cell lymphomas and leukemias, such as chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL; n = 15), mantle cell lymphoma (MCL; n = 15), low-grade follicular lymphoma (FL; n = 44), hairy cell leukemia (HCL; n = 15), and reactive lymphoid hyperplasia (n = 14). NOTCH2 mutations were associated with adverse clinical outcomes (relapse, histological transformation, and/or death) among SMZL patients (P = 0.002). These results suggest that NOTCH2 mutations play a role in the pathogenesis and progression of SMZL and are associated with a poor prognosis.

Pseudohypopyon as a Clinical Manifestation in Metastatic Lung Carcinoma

Pseudohypopyon is a rare clinical presentation of meta-static disease in the eye. The authors report a case of anterior chamber pseudohypopyon in a 74-year-old man with a history of primary central nervous system and spleen lymphoma, as well as lung, prostate, and bladder carcinoma. A diagnostic vitrectomy and anterior chamber paracentesis of the involved eye was performed that confirmed non-small-cell carcinoma of lung primary.

膿瘍と鑑別困難であった脾嚢胞を伴った脾原発Ki-1 Lymphomaの1例

膿瘍と鑑別困難であった脾嚢胞を伴った脾原発Ki-1 Lymphomaの1例

Hepatosplenic T‐cell lymphoma is a distinct rare entity: Diagnosis by fine‐needle aspiration cytology

In this brief report, we have described the fine-needle aspiration cytology (FNAC) of a hepatosplenic T-cell lymphoma (HSTCL) in a 60-year-old female patient. The patient complained of fever and heaviness of left upper abdomen. Ultrasound-guided FNAC of the spleen showed immature lymphoid cells, and a diagnosis of primary non-Hodgkin lymphoma of spleen was offered. Subsequently, the patient underwent splenectomy and wedge biopsy of liver that confirmed the cytological diagnosis. The lymphoma cells were positive for CD3 immunostain. Primary hepatosplenic T-cell lymphoma is a rare entity and may often pose diagnostic problems on FNAC.

Radiation-Associated Kidney Injury

The kidneys are the dose-limiting organs for radiotherapy to upper abdominal cancers and during total body irradiation. The incidence of radiotherapy-associated kidney injury is likely underreported owing to its long latency and because the toxicity is often attributed to more common causes of kidney injury. The pathophysiology of radiation injury is poorly understood. Its presentation can be acute and irreversible or subtle, with a gradual progressive dysfunction over years. A variety of dose and volume parameters have been associated with renal toxicity and are reviewed to provide treatment guidelines. The available predictive models are suboptimal and require validation. Mitigation of radiation nephropathy with angiotensin-converting enzyme inhibitors and other compounds has been shown in animal models and, more recently, in patients.