Lycopersicum Plants Research Articles

Cell-cell communication and initial population composition shape the structure of potato spindle tuber viroid quasispecies.

RNA viruses and viroids replicate with high mutation rates, forming quasispecies, population of variants centered around dominant sequences. The mechanisms governing quasispecies remain unclear. Plasmodesmata regulate viroid movement and were hypothesized to impact viroid quasispecies. Here, we sequenced the progeny of potato spindle tuber viroid intermediate (PSTVd-I) strain from mature guard cells lacking plasmodesmal connections and from in vitro-cultivated mesophyll cell protoplasts from systemic leaves of early-infected tomato (Solanum lycopersicum) plants. Remarkably, more variants accumulated in guard cells compared to whole leaves. Similarly, after extended cell culture, we observed more variants in cultivated mesophyll protoplasts. Coinfection and single-cell sequencing experiments demonstrated that the same plant cell can be infected multiple times by the same or different PSTVd sequences. To study the impact of initial population composition on PSTVd-I quasispecies, we conducted coinfections with PSTVd-I and variants. Two inoculum ratios (10:1 or 1:10) established quasispecies with or without PSTVd-I as the master sequence. In the absence of the master sequence, the percentage of novel variants initially increased. Moreover, a 1:1 PSTVd-I/variant RNA ratio resulted in PSTVd-I dominating (>50%), while the variants reached 20%. After PSTVd-I-only infection, the variants reached around 10%, while after variant-only infection, the variants were significantly more than 10%. These results emphasize the role of cell-to-cell communication and initial population composition in shaping PSTVd quasispecies.

Effect of Vermicompost and Organic Fertilizer on Improved Growth, Productivity and Quality of Tomato (Solanum lycopersicum ) Plant

Effect of Vermicompost and Organic Fertilizer on Improved Growth, Productivity and Quality of Tomato (Solanum lycopersicum ) Plant

Untargeted metabolomics reveals PTI-associated metabolites.

Plants employ a multilayered immune system to combat pathogens. In one layer, recognition of Pathogen- or Microbe-Associated Molecular Patterns or elicitors, triggers a cascade that leads to defence against the pathogen and Pattern Triggered Immunity. Secondary or specialised metabolites (SMs) are expected to play a role, because they are potentially anti-fungal compounds. Tomato (Solanum lycopersicum) plants inoculated with Alternaria solani s.l. show symptoms of infection after inoculation. Plants inoculated with Alternaria alternata remain symptomless. We hypothesised that pattern-triggered induction of resistance related metabolites in tomato contributes to the resistance against A. alternata. We compared the metabolomic profile (metabolome) of tomato after treatments with A. alternata, A. solani and the fungal elicitor chitin, and identified SMs involved in early defence of tomato plants. We revealed differential metabolome fingerprints. The composition of A. alternata and chitin induced metabolomes show larger overlap with each other than with the A. solani induced metabolome. We identify 65 metabolites possibly associated with PTI in tomato plants, including NAD and trigonelline. We confirm that trigonelline inhibits fungal growth in vitro at physiological concentrations. Thus, a true pattern-triggered, chemical defence is mounted against A. alternata, which contains anti-fungal compounds that could be interesting for crop protection strategies.

Effect of Farming Methods on Solanum Lycopersicum Plant

The cultivation of Solanum lycopersicum, commonly known as tomato, is a critical aspect of global agriculture, with various farming methods employed to enhance plant growth and yield. This project investigates the effect of different farming methods, including organic and conventional practices, on the growth, development, and yield of Solanum lycopersicum plants. The study utilizes a comparative approach, analyzing key parameters such as plant height, leaf area, fruit weight, and nutrient content. The results demonstrate that organic farming methods significantly influence the growth and yield of Solanum lycopersicum plants compared to conventional methods. Organic farming promotes sustainable agricultural practices, enhances soil health, and reduces environmental impact. The findings of this study contribute to the ongoing discourse on sustainable farming practices and their impact on crop productivity, highlighting the importance of organic farming in ensuring food security and environmental sustainability

Interaction with the entomopathogenic fungus Beauveria bassiana influences tomato phenome and promotes resistance to Botrytis cinerea infection.

Plants are central to complex networks of multitrophic interactions. Increasing evidence suggests that beneficial microorganisms (BMs) may be used as plant biostimulants and pest biocontrol agents. We investigated whether tomato (Solanum lycopersicum) plants are thoroughly colonized by the endophytic and entomopathogenic fungus Beauveria bassiana, and how such colonization affects physiological parameters and the phenotype of plants grown under unstressed conditions or exposed to the pathogenic fungus Botrytis cinerea. As a positive control, a strain of the well-known biocontrol agent and growth inducer Trichoderma afroharzianum was used. As multitrophic interactions are often driven by (or have consequences on) volatile organic compounds (VOCs) released by plants constitutively or after induction by abiotic or biotic stresses, VOC emissions were also studied. Both B. bassiana and T. afroharzianum induced a significant but transient (one to two-day-long) reduction of stomatal conductance, which may indicate rapid activation of defensive (rejection) responses, but also limited photosynthesis. At later stages, our results demonstrated a successful and complete plant colonization by B. bassiana, which induced higher photosynthesis and lower respiration rates, improved growth of roots, stems, leaves, earlier flowering, higher number of fruits and yield in tomato plants. Beauveria bassiana also helped tomato plants fight B. cinerea, whose symptoms in leaves were almost entirely relieved with respect to control plants. Less VOCs were emitted when plants were colonized by B. bassiana or infected by B. cinerea, alone or in combination, suggesting no activation of VOC-dependent defensive mechanisms in response to both fungi.

An Application of System Dynamics to Characterize Crop Production for Autonomous Indoor Farming Platforms (AIFP)

Smart farming using technology-monitored controlled environment agriculture (CEA) has recently evolved to optimize crop growth while minimizing land use and environmental impacts, especially for climate-threatened regions. This study focuses on characterizing crop production using system dynamics (SD) modeling, which is a relatively new approach in CEA settings. Using tomatoes in a hydroponic growing system, we explore an alternative food resource potentially accessible to underserved areas in rural and/or urban settings under abrupt climate variability. The designed autonomous indoor farming platforms (AIFP) are equipped with the Internet of Things (IoT) to monitor the physiological parameters, including electrical conductivity (EC), pH, and water temperature (WT) associated with plant growth. Two varieties of tomato (Solanum lycopersicum) plants were used in this study with two different nutrient inputs (N-P-K ratios of 2-1-6 and 5-5-5) to assess the nutrient application impact on yield, especially focusing on the early stages of tomato to conceptualize and parametrize SD modes. Repeated measure analysis was conducted to investigate the effects of the environmental factors (EC, pH, and WT) in response to changing plant nutrients. The results show that different nutrient compositions (N-P-K ratios) have a noticeable effect on both pH and WT (p < 0.001) as opposed to EC. The study indicates that the proposed AIFP would be a promising solution to produce other crops for indoor farming in a changing climate. We anticipate that the proposed AIFP along with SD tools will be widely adopted to promote indoor farming in changing climates, ultimately contributing to community resilience against food insecurity in disadvantaged areas for years to come.

Using Citizen Science to Evaluate Home Gardeners’ Experiences with Compact Tomato Plants

Understanding preferences and challenges of home gardeners is valuable to the consumer-horticulture industry. Citizen scientists in Florida were recruited to grow compact tomato (Solanum lycopersicum) plants started from seed, as transplants, or as flowering plants in a 16-week experiment. Participants, who had various gardening experience levels, were provided with a kit containing all materials needed to grow plants to maturity. Project engagement was encouraged with monthly online meetings and a social media page. A survey was delivered at the end of the project and completed by 117 participants. The survey aimed to evaluate participants’ preferences, challenges, and experiences with each plant product. Plants started as seed or as flowering plants were equally preferred among participants and were rated higher than transplants. However, participants were least satisfied with the yield, rate of plant growth, fruit taste, and care required to grow plants started from seed. Ninety-one percent of participants said they would be willing to pay more for flowering plants than for transplants. Across plant products, pests and flower/fruit drop were reported as challenges by up to 85% and 18% of participants, respectively. Results from this study highlight the potential of using citizen science to assess gardening experiences and preferences, which can support stakeholders who cater to the consumer-horticulture industry.

Detection and Classification of Plant Diseases in Crops (Solanum lycopersicum) due to Pests Using Deep Learning Techniques: A Review

All people have access to food thanks to agriculture, even in areas with rapid population expansion. In order to ensure that the entire population has access to food, early diagnosis of plant diseases is recommended. However, when crops are still maturing, it might be challenging to predict diseases. The goal of this paper is to inform farmers about the use of machine learning to recognize various pest species in various crop plants. Deep learning establishes a continuing, cutting-edge method for analysing images with significant potential and promising outcomes. DL has expanded into the field of agriculture after demonstrating its effectiveness in a number of applications. Here, we reviewed various research articles that used deep learning methods to address diverse research issues in tomato (Solanum lycopersicum) plants. We look at the study areas in tomato plants where deep learning, data preprocessing, transfer learning, and augmentation approaches are performed. studied dataset details, including the number of photos, classes, and train test to validation ratios that were employed. We also look at comparisons made across different deep learning architectures and analyse the results. The results demonstrated that deep learning techniques beat all other image processing methods, however how well DL works depended on the dataset employed.

Quantification of Virion-Sense and Complementary-Sense DNA Strands of Circular Single-Stranded DNA Viruses.

Circular ssDNA viruses are ubiquitous and can be found in both prokaryotes and eukaryotes. To understand the interaction of ssDNA viruses with their hosts, it is important to characterize the dynamics of viral sense (VS) and complementary-sense (CS) viral strands during the infection process. Here, we present a simple and rapid protocol that allows sensitive and accurate determination of the VS and CS strands generated during viral infection.The method consists of a two-step qPCR in which the first step uses a strand-specific (CS or VS) labeled primer and T4 DNA polymerase that lacks strand displacement activity and makes a single copy per VS or CS strand. Next, the T4 DNA polymerase and unincorporated oligonucleotides are removed by a silica membrane spin column. Finally, the purified VS or CS strands are quantified by qPCR in a second step in which amplification uses a tag primer and a specific primer. Absolute quantification of VS and CS strands is obtained by extrapolating the Cq data to a standard curve of ssDNA, which can be generated by phagemid expression. Quantification of VS and CS strands of two geminiviruses in infections of Solanum lycopersicum (tomato) and Nicotiana benthamiana plants using this method is shown.

Uncovering the Role of Hormones in Enhancing Antioxidant Defense Systems in Stressed Tomato (Solanum lycopersicum) Plants.

Tomato is one of the most important fruits worldwide. It is widely consumed due to its sensory and nutritional attributes. However, like many other industrial crops, it is affected by biotic and abiotic stress factors, reducing its metabolic and physiological processes. Tomato plants possess different mechanisms of stress responses in which hormones have a pivotal role. They are responsible for a complex signaling network, where the antioxidant system (enzymatic and non-enzymatic antioxidants) is crucial for avoiding the excessive damage caused by stress factors. In this sense, it seems that hormones such as ethylene, auxins, brassinosteroids, and salicylic, jasmonic, abscisic, and gibberellic acids, play important roles in increasing antioxidant system and reducing oxidative damage caused by different stressors. Although several studies have been conducted on the stress factors, hormones, and primary metabolites of tomato plants, the effect of endogenous and/or exogenous hormones on the secondary metabolism is still poorly studied, which is paramount for tomato growing management and secondary metabolites production. Thus, this review offers an updated overview of both endogenous biosynthesis and exogenous hormone application in the antioxidant system of tomato plants as a response to biotic and abiotic stress factors.

Use of marine minerals obtained through an innovative process with added silver for the cultivation and protection of Solanum lycopersicum

Research objective: The aim of this research was to evaluate the stimulating potential of (FertilTomix: a product based on minerals and organic molecules from the sea) obtained from an innovative extraction process and its ability to increase plant defence capabilities following the addition of silver to seeded Solanum lycopersicum plants. Materials and Methods: The experiments, which started in January 2023, were conducted in the CREA-OF greenhouses in Pescia (Pt), Tuscany, Italy (43°54′N 10°41′E) on Solanum lycopersicum cv “Siccagno”. The plants were placed in ø 24 cm pots; 30 plants per thesis, divided into 3 replicas of 10 plants each. The experimentation was divided into two parallel trials, one concerning the growth and biostimulation of tomato plants. The second experiment, instead, concerned the use of sprayed FertilTomix in the protection from pathogenic fungi (Phytophthora infestans, Leivellula taurica). On August 28, 2023, plant height (measured at 70 days after transplanting), plant nodes (measured at 70 days after transplanting), leaf area index, total dry biomass, fruit fresh weight and total fruit number have been evaluated. In addition, plants affected by diseases were counted. Results and Discussion: The experiment showed that the use of FertilTomix, irrespective of the extraction process, was able to significantly increase total plant growth, berry yield and weight, and reduce the mortality of Solanum lycopersicum plants. As found in the experiment in the treated theses, a significant increase in plant height and number of nodes per plant was noted, as well as a significant increase in total LAI. Differences were also noted between FertilTomix as such and FertilTomix enriched with silver; the former product worked better in the vegetative and root stimulation of the plants, while the product enriched with silver defended the plants better against attacks by Phytipthora infestans and Leivellula taurica. The use of this product is very interesting especially for those places where there is no water for irrigation and where fertilisers are scarce. The use of this product could obviate both problems. There are as yet no cost-effective technologies and processes in the world that can extract useful minerals from seawater for plant fertilisation and recycle the process water that can be used for irrigation. Conclusions: The test demonstrated a significant effect on plant growth after the application of certain extracts obtained from an innovative process of extracting minerals from seawater. In addition, it emerged that the product could also be used for plant protection when enriched with silver. This product would be very useful especially in countries where water resources and fertilisers are scarce, with the possibility of producing them on site through innovative facilities.

Study on the impact of exogenously applied methyl jasmonate concentrations on Solanum lycopersicum under low temperature stress

BackgroundTo decipher the capability of Methyl Jasmonate (MeJA) in resisting cold stress in Solanum lycopersicum assessment regarding various physiological parameters in response to diverse doses of MeJA was done. Low temperature (LT) were given to the plants with MeJA (J1C, J2C, J3C) or without MeJA (LT) application. MeJA in the form of foliar spray was given before stress, during stress and after stress. Three concentrations of MeJA were used under normal and LT stress conditions that includes of J1 (0.5 µM), J2 (10 µM), and J3 (15 µM).ResultsOxidative stress, growth characteristics, stress tolerance parameters, antioxidant response and photosynthetic parameters were investigated. In our current study we observed that oxidative stress markers declined by MeJA supplementation under cold stress conditions. MeJA boosted antioxidant enzyme activity along with photosynthetic parameters. The best concentration of MeJA was J2 based on results obtained. This is the first study related to MeJA best dose screening in Solanum lycopersicum under LT stress conditions.ConclusionThe LT stress in the Solanum lycopersicum plant was reduced by MeJA. The adverse consequences of LT stress can be significantly attenuated by the J2 concentration of MeJA. So, the optimal concentration of MeJA supplied exogenously to LT stressed Solanum lycopersicum can be a smart strategy to mitigate harmful impact of LT stress on detox system and overall growth of plant.

Characterization of three new plant growth-promoting microbes and effects of the interkingdom interactions on plant growth and disease prevention.

The novel interkingdom PGPM consortia enhanced the ability of plant growth promotion and disease resistance, which would be beneficial to improve plant growth in sustainable agriculture through engineering microbiome. Plant growth-promoting microbes (PGPMs) play important roles in promoting plant growth and bio-controlling of pathogens. Much information reveals that the plant growth-promoting ability of individual PGPM affects plant growth. However, the effects of the PGPM consortia properties on plant growth remain largely unexplored. Here, we characterized three new PGPM strains including Rhodotorula graminis JJ10.1 (termed as J), Pseudomonas psychrotolerans YY7 (termed as Y) and P. chlororaphis T8 (termed as T), and assessed their effects in combination with Bacillus amyloliquefaciens FZB42 (termed as F) on plant growth promotion and disease prevention in Arabidopsis thaliana and tomato (Solanum lycopersicum) plants by investigating morphological changes, whole-genome sequencing and plant growth promoting (PGP) characterization. Results revealed that the three new strains R. graminis JJ10.1, P. psychrotolerans YY7 and P. chlororaphis T8 had the potential for being combined with B. amyloliquefaciens FZB42 to form interkingdom PGPM consortia. The combinations of R. graminis JJ10.1, B. amyloliquefaciens FZB42, and P. psychrotolerans YY7, i. e. JF and JYF, exhibited the strongest ability of synergetic biofilm production. Furthermore, the growth-promotion abilities of the consortia were significantly enhanced compared with those of individual strains under both inoculation and volatile organic compounds (VOCs) treatment. Importantly, the consortia showed stronger abilities of in planta disease prevention than individual strains. Findings of our study may provide future guidance for engineering the minimal microbiome communities to improve plant growth and/or disease resistance in sustainable agriculture.

Physiological and metabolic changes in response to Boron levels are mediated by ethylene affecting tomato fruit yield

Boron (B) is an essential nutrient for the plant, and its stress (both deficiency and toxicity) are major problems that affect crop production. Ethylene metabolism (both signaling and production) is important to plants’ differently responding to nutrient availability. To better understand the connections between B and ethylene, here we investigate the function of ethylene in the responses of tomato (Solanum lycopersicum) plants to B stress (deficiency, 0 μM and toxicity, 640 μM), using ethylene related mutants, namely nonripening (nor), ripening-inhibitor (rin), never ripe (Nr), and epinastic (Epi). Our results show that B stress does not necessarily inhibit plant growth, but both B stress and ethylene signaling severely affected physiological parameters, such as photosynthesis, stomatal conductance, and chlorophyll a fluorescence. Under B toxicity, visible symptoms of toxicity appeared in the roots and margins of the older leaves through necrosis, caused by the accumulation of B which stimulated ethylene biosynthesis in the shoots. Both nor and rin (ethylene signaling) mutants presented similar responses, being these genotypes more sensitive and displaying several morphophysiological alterations, including fruit productivity reductions, in response to the B toxicity conditions. Therefore, our results suggest that physiological and metabolic changes in response to B fluctuations are likely mediated by ethylene signaling.

Modulation of ethylene and ROS-scavenging enzymes by multifarious plant growth-promoting endophytes in tomato (Solanum lycopersicum) plants to combat Xanthomonas -induced stress

The purpose of the current study was to explore root endophytes- Priestia megaterium T3 and Bacillus cereus T4 from Moringa olefiera for the suppression of leaf spot disease in tomato plants challenged with Xanthomonas vesicatoria. Both strains had plant growth-stimulating characteristics including auxin production, solubilization of inorganic phosphate and zinc complexes, and production of ammonia, siderophore, as well as hydrolytic enzymes. An agar well diffusion and fluorescence viability assay have validated the antibacterial effect of the cell-free culture supernatant of strains T3 and T4. Liquid chromatography-mass spectrometry (LC-MS) profiling has identified the secondary metabolites in the cell-free supernatant of strains T3 and T4. The bio-priming of tomato seeds with a consortium of T3 and T4 strains has significantly declined ethylene (by 0.61-fold) and hydrogen peroxide (H2O2, 0.64-fold) concentration thus, maintaining a lower content of ROS-induced malondialdehyde (MDA, 0.91-fold) as compared to control counterparts. Consequently, the leaf spot disease severity was reduced by ∼70% in consortium-treated tomato plants in contrast to their pathogen-challenged control. The consortia (T3+T4) treatment has facilitated induced systemic resistance by enhancing enzymatic activities of phenylalanine ammonia-lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), catalase (CAT), and ascorbate oxidase (AO) to detoxify the excessive Xanthomonas-induced ROS accumulation in tomato plants. Conclusively, bacterial endophytes modulate X. vesicatoria-induced ROS response and ethylene levels in tomato plants. The current findings indicate that plant growth-promoting endophytic bacterial strains hold the potential to sustainably enhance plant growth and suppress bacterial leaf spot disease in tomato plants.

Tomato Aphid (Aphis gossypii) Secreted Saliva Can Enhance Aphid Resistance by Upregulating Signaling Molecules in Tomato (Solanum lycopersicum).

This study investigated the impact of Aphis gossypii watery saliva on the induction of tomato (Solanum lycopersicum) plant resistance. To examine the role of A. gossypii saliva, we collected watery saliva from A. gossypii after a 48 h feeding period on an artificial diet. SDS-PAGE resolving gel 12% was used to separate the salivary proteins. Relative expression of gene analysis revealed that the intrusion of A. gossypii saliva dripping onto S. lycopersicum leaves triggered robust defense responses mediated by a signaling molecule, i.e., salicylic acid, while the signaling molecule's jasmonic acid-dependent defense responses were moderately activated. Aphid saliva infiltrated S. lycopersicum leaves slowed the intrinsic rate of population growth of A. gossypii and significantly reduced the number of nymphs produced daily, compared to untreated leaves. During a choice test with untreated S. lycopersicum, aphids showed a repellent response towards saliva-infiltrated S. lycopersicum. Moreover, the (EPG) electrical penetration graph analysis demonstrated that the eating pattern of A. gossypii compared to untreated S. lycopersicum, that had been exposed to saliva was negatively impacted. These results provide compelling evidence for the involvement of salivary components of A. gossypii in inducing resistance against aphids in S. lycopersicum plants. Furthermore, the study underscores the crucial role of watery saliva in the intricate interactions between aphids and plants. The activation of pathways was also part of the defensive response (jasmonic acid (JA), salicylic acid (SA) signaling molecules). The findings of this research deliver valuable insights into the potential of watery aphid saliva as a natural defense mechanism against aphid infestations in S. lycopersicum crops.

Deciphering the synergistic effect of Jasmonic acid and Spermine in mitigating root-knot nematode stress in tomato plants through enhancing growth and activity of antioxidant enzymes

Root-Knot nematodes (RKNs) are one of the major plant pathogens that can substantially reduce plant growth and development. Management of RKNs is one of the major concerns as chemical pesticides pose a potential risk to humans and other life forms and also have toxic effects to environment. There is a need to find out some alternative eco-friendly strategies for management of these parasites. Jasmonic acid (JA) and Spermine (Spm) are those plant growth regulators (PGRs) that have positive effects on plant growth and alleviates toxic effects caused by various stresses including RKNs. Keeping this in mind,present study accessed the potential of these (PGRs), JA and Spm on morphological and biochemical attributes in 45 days old Solanum lycopersicum plants under nematode stress. It was observed that nematode infection resulted in reduced plant growth and photosynthetic pigments. Application of JA and Spm improved growth parameters and photosynthetic pigments. Moreover, tomato plants after JA and Spm supplementation lowered the activities of H2O2, DPPH and MDA thus decreasing nuclear and membrane damage caused by nematode stress. Additionally, enzymatic CAT (Catalase), SOD (Superoxide dismutase), GuPOX (Guaiacol peroxidase), APOX (Ascorbate peroxidase), PPO (Polyphenol oxidase), GST (Glutathione-S-transferase) and non-enzymatic (ascorbic acid, glutathione, and tocopherol) antioxidant levels increased markedly under nematode stress, but application of JA and Spm further improved these attributes. Taken together, the current study demonstrated that JA and Spm are suitable agents for managing nematode stress resistance in tomato plants.

The transcription factor SPL13 mediates strigolactone suppression of shoot branching by inhibiting cytokinin synthesis in Solanum lycopersicum.

Plant architecture imposes a large impact on crop yield. IDEAL PLANT ARCHITECTURE 1 (IPA1), which encodes a SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor, is a target of molecular design for improving grain yield. However, the roles of SPL transcription factors in regulating tomato (Solanum lycopersicum) plant architecture are unclear. Here, we show that the expression of SPL13 is down-regulated in the lateral buds of strigolactone (SL)-deficient ccd mutants and is induced by GR24 (a synthetic analog of SL). Knockout of SPL13 by CRISPR/Cas9 resulted in higher levels of cytokinins (CKs) and transcripts of the CK synthesis gene ISOPENTENYL TRANSFERASES 1 (IPT1) in the stem nodes, and more growth of lateral buds. GR24 suppresses CK synthesis and lateral bud growth in ccd mutants, but is not effective in spl13 mutants. On the other hand, silencing of the IPT1 gene inhibited bud growth of spl13 mutants. Interestingly, SL levels in root extracts and exudates are significantly increased in spl13 mutants. Molecular studies indicated that SPL13 directly represses the transcription of IPT1 and the SL synthesis genes CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7) and MORE AXILLARY GROWTH 1 (MAX1). The results demonstrate that SPL13 acts downstream of SL to suppress lateral bud growth by inhibiting CK synthesis in tomato. Tuning the expression of SPL13 is a potential approach for decreasing the number of lateral shoots in tomato.

Significant impact on the growth of Solanum lycopersicum in Microbially augmented organic waste

The objectives of this study were to convert organic and tea waste to organic soil cond conditioners and achieve a 68% reduction in landfilling and illegal dumping of organic waste. The tea was mixed at an incremental ratio of 10% with the organic waste, and the groups were designated as T0-T100 groups, indicating 0% of tea waste and 100% of organic waste in T0 group and 100% of tea waste, and 0% of organic waste in T100 group. The impact of this soil conditioner was later studied on the growth of the Solanum lycopersicum (tomato) plant for twenty-one weeks along with the augmentation of waste sludge from the yeast manufacturing unit. Microbial examination, and other physicochemical parameters like pH, temperature, organic carbon, C/N ratio, moisture, cation exchange capacity (CEC), and humidity of the compost were studied. A visual color change indicated the compost maturity for further application. The quality of the soil was analyzed in both pre-compost and post-compost applications. The electrical conductivity of the soil was 3.0 mho with 8.3 pH, 0.84 % organic matter, phosphorous 1.0 mg/kg, and potassium 225 mg/kg with a saturation level of 40%. There was an inverse relation between organic waste and organic matter with the highest percentage of organic matter in the T0 group. Maximum nitrogen content (9.07%), the highest levels of phosphorous (0.7%), and potassium (2.3%) were recorded in the aerobic T0 group. The highest amount of organic carbon (78.23%), maximum CEC (109.09 meq/100g) was found in the aerobic T100 group. The best vegetative post-application growth of tomato plants, flowering, and fruiting was observed in the T60 group. Taken together with our findings, it can be concluded that the organic compost boosted the soil fertility by up to 70% which positively affected the growth of tomato plants.

Vanillin in Resistant Tomato Plant Root Exudate Suppresses Meloidogyne incognita Parasitism.

Tomato (Solanum lycopersicum) plants are susceptible to infection by root-knot nematodes, which cause severe economic losses. Planting resistant tomato plants can reduce nematode damage; however, the effects of resistant tomato root exudates in suppressing Meloidogyne incognita remain insufficiently understood. Here, we determined that the resistant tomato plant Lycopersicon esculentum cv. Xianke-8 (XK8) alleviates nematode damage by downregulating the expression of the essential parasitic nematode gene Mi-flp-18 to reduce the infection and reproduction of M. incognita. Using gas chromatography-mass spectrometry, we identified vanillin as a unique compound (compared to susceptible tomato cultivars) in XK8 root exudates that acts as a lethal trap and inhibitor of egg hatching. Moreover, the soil application of 0.4-4.0 mmol/kg vanillin significantly reduced galls and egg masses. The parasite gene Mi-flp-18 was downregulated upon treatment with vanillin, both in vitro and in pot experiments. Collectively, our results reveal an effective nematicidal compound that can use in feasible and economical strategies to control RKNs.