Luzhou Medical College Research Articles

Codelivery of thioridazine and doxorubicin using nanoparticles for effective breast cancer therapy.

Cancer chemotherapy can benefit from the combination of different anticancer drugs. Here, we prepared doxorubicin (Dox)- and thioridazine (Thio)-coloaded methoxy poly(ethylene glycol)-poly(l-lactic acid) (MPEG-PLA) nanoparticles (NPs) for breast cancer therapy. These NPs have an average particle size of 27 nm. The drug loading efficiencies of Thio and Dox are 4.71% and 1.98%, respectively. Compared to the treatment of Thio or Dox alone, the combination of Thio and Dox exhibited a synergistic effect in inhibiting the growth of 4T1 breast cancer cells in vitro. In addition, the Thio- and Dox-coloaded MPEG-PLA NPs could efficiently suppress the growth of breast cancer cells in vivo. This study suggests that Thio- and Dox-coloaded MPEG-PLA NPs might have potential applications in breast cancer treatment.

Construction of p66Shc gene interfering lentivirus vectors and its effects on alveolar epithelial cells apoptosis induced by hyperoxia.

BackgroundThe aim of this study is to observe the inhibitive effects of p66Shc gene interfering lentivirus vectors on the expression of p66Shc, and to explore its effects on alveolar epithelial cells apoptosis induced by hyperoxia.MethodsThe gene sequences were cloned into the pLenR-GPH-shRNA lentiviral vector, which was selected by Genebank searches. The pLenR-GPH-shRNA and lentiviral vector packaging plasmid mix were cotransfected into 293T cells to package lentiviral particles. Culture virus supernatant was harvested, and then the virus titer was determined by serial dilution assay. A549 cells were transduced with the constructed lentiviral vectors, and real-time polymerase chain reaction (RT-PCR) and Western blot were used to evaluate p66Shc expression. This study is divided into a control group, a hyperoxia group, an A549-p66ShcshRNA hyperoxia group, and a negative lentivirus group. Cell apoptosis was detected by flow cytometry after 24 hours; the expression of X-linked inhibitor of apoptosis protein (XIAP) and caspase-9 were detected by immunohistochemistry assay. The production of reactive oxygen species and cellular mitochondria membrane potential (ΔΨm) were determined by fluorescence microscopy.ResultsWe successfully established the p66Shc gene interfering lentivirus vectors, A549-p66ShcshRNA. The A549-p66ShcshRNA was transfected into alveolar epithelial cells, and the inhibitive effects on the expression of p66Shc were observed. Both RT-PCR and Western blot demonstrated downregulation of p66Shc expression in A549 cells. In the A549-p66ShcshRNA hyperoxia group, we found dampened oxidative stress. A549-p66ShcshRNA can cause p66Shc gene silencing, reduce mitochondrial reactive oxygen species generation, reduce membrane potential decrease, reduce the apoptosis of A549 cells, and reduce alveolar epithelial cell injury, while the lentiviral empty vector group had no such changes.Conclusionp66Shc gene interfering lentivirus vector can affect the alveolar epithelial cells apoptosis induced by hyperoxia.

Relation Between Severity of Injury and Neurological Deficit in Thoracolumbar Burst Fracture

A prospective, consecutive case series. To evaluate which variable has a strong association with neurological damage in thoracolumbar burst fractures (T12 or L1): severity of injury or narrowing of the spinal canal. Department of Spine Surgery, the Affiliated Hospital, Luzhou Medical College, Sichuan Province, China. This study included 42 patients with thoracolumbar junction burst fractures (T12 or L1), 24 patients with neurological deficit, and 18 patients without deficit. The severity of injury was determined using the Injury Severity Score (ISS) and the New Injury Severity Score (NISS), and the narrowing of the spinal canal was measured by axial computed tomography. The statistical comparison of the neurological deficit and the non-neurological deficit was carried out using nonparametric tests (Mann-Whitney test). The correlation of these scores and narrowing of the spinal canal to the neurological status was determined using a Spearman correlation test. Higher ISS (21 vs. 11), NISS (30 vs. 15), Injury Severity Score with modification (ISSM) (13 vs. 11), New Injury Severity Score with modification (NISSM) (21 vs. 15), and canal compromise (48 vs. 25) were found in patients with neurological deficit, which evidently yielded statistical differences between the patients with neurological deficit and those without (P<0.01), and they also had a positive correlation with the American Spine Injury Association (ASIA) score (r>0.5, P<0.001). Moreover, there was also a positive correlation (r=0.375, P<0.05) between ISSM and ASIA scores, although it seemed weak. The correlations between the NISS, NISSM, and neurological deficit (respectively r=0.868, P<0.001; r=0.676, P<0.001) were stronger than with the narrowing of the spinal canal (r=0.560, P<0.001). NISS have a closer correlation with neurological deficit in thoracolumbar burst fractures; thus, we can put forward a hypothesis that perhaps NISS is able to reflect the dynamic fracture process.

En bloc resection for treatment of refractory pre-auricular fistula

ObjectiveTo report a surgical method for the treatment of pre-auricular fistula to lower post-operative recurrence rate. MethodsClinical data of 187 patients with pre-auricular fistula who underwent en bloc resection at the Affiliated Hospital of Luzhou Medical College from August 2006 to November 2012 were retrospectively reviewed. Factors that might affect the prognosis following En bloc fistula resection bordered by the superficial temporalis fascia, helix perichondrium and auriculocephalic sulcus were investigated. ResultsOf the 187 patients, 181 achieved primary healing and 6 ended up with delayed healing. During the follow-up period (one to seven years), there were 4 cases of recurrence (2.1%). ConclusionsClear demarcation of surgical resection can facilitate easy and thorough resection of preauricular fistula and lead to low recurrence rate. Proper timing and careful search for potential fistula branches are the two crucial factors affecting prognosis.

Application value of sleeve pancreaticojejunostomy in digestive tract reconstruction

Objective To investigate the application value of sleeve pancreaticojejunostomy in digestive tract reconstruction. Methods The clinical data of 40 patients who underwent sleeve pancreaticojejunostomy at the Affiliated Hospital of Luzhou Medical College between July 2013 and February 2015 were retrospectively analyzed. The head of pancreas and duodenal descending part were drifted out through Kouch technique, sections were sutured in braid-like U shape and the small pancreatic ducts were closed. The posterior wall of pancreatic stump and jejunal wall were sutured routinely and main pancreatic tube was implanted by silica gel with the thrum retained. The reserved lines were gone through at 6 o'clock and 12 o'clock in the jejunal wall to be sutured and knotted, making the jejunal mucosa set on the surface of main pancreatic duct like sleeve, and then the anterior wall pancreatic stump was sutured. The intraoperative situation and amylase concentration of drainage at post-operative day 1, 3, 5, 7 were observed and monitored. The patients were followed up for liver function, tumor markers, abdominal color Doppler ultrasound every 3 months and enhanced abdominal computer tomography every 6 months till May 2015. Measurement data with skewed distribution were presented as M(Qn) and analyzed by rank-sum test. Measurement data with normal distribution data were presented as ±s. Repeated measurement data were analyzed by the repeated measures ANOVA. Results All the 40 patients underwent sleeve pancreaticojejunostomy successfully. The duration of operation and pancreaticojejunostomy were (6.2±1.3) hours and (15±6) minutes, respectively. The volume of intraoperative blood loss was (370±55)mL and diameter of tumor was (2.5±1.5)cm. The amylase concentrations of drainage at postoperative day 1, 3, 5, 7 were 46.300 U (35.575 U, 68.600 U), 34.900 U (21.050 U, 55.550 U), 26.750 U (0.000 U, 41.400 U) and 0.000 U(0.000 U, 30.500 U), showing decrease trend with the time gone (F=97.268, P<0.05). Among the 40 patients, 2 had increase of drainage amylase concentration at anastomotic stoma with the drain amount less than 50 mL/d, showing normal temperature, no signs of abdominal pain and peritonitis and potential pancreatic leakage, they were recovered after symptomatic and supportive treatments. The other patients had no complications such as anastomotic leakage and hemorrhage. The duration of hospital stay of 40 patients was (18±5)days. Thirty-nine patients were followed up for 3-15 months, showing no postoperative anastomotic stenosis, acute or chronic pancreatitis and pancreatolithiasis, 1 of whom with recurrence tumor. Conclusion Sleeve pancreaticojejunostomy is easy to master, safe and reliable, and it is suitable for soft pancreatic tissue. Key words: Pancreatic diseases; Pancreaticoduodenectomy; Sleeve pancreaticojejunostomy

Effects of total nutrient admixture on the recovery of patients with gastric cancer after radical gastrectomy

Objective To investigate the effects of total nutrient admixture (TNA) on the recovery of patients with gastric cancer after radical gastrectomy. Methods The clinical data of 50 patients with gastric cancer who were admitted to the Affiliated Hospital of Luzhou Medical College between March 2013 and March 2014 were retrospectively analyzed. Among 50 patients receiving radical gastrectomy, 26 patients receiving TNA were allocated to the experimental group and 24 patients receiving conventional fluid infusion were allocated to the control group. Patients in the experimental group received the nutritional support therapy using TNA at preoperative day 5 and at postoperative days 1- 5, and patients in the control group received the postoperative intravenous rehydration including water, glucose, electrolyte, vitamins and micro elements. The nutritional indexes [albumin (Alb) , prealbumin, transferrin and hemoglobin (Hb) ] , time to anal exsufflation, incidence of complications (wound infection, anastomotic leakage, blooding and intestinal obstruction) and duration of hospital stay were observed before nutritional support therapy and at postoperative day 8. The count data were analyzed using the chi-square test. The chi-square value of correction for continuity was used when 1≤minimum theoretical frequency≤5. The measurement data with normal distribution were presented as ±s and analyzed using thet test or repeated measures ANOVA. The ordinal data were analyzed by the analysis of variance. Results The Alb, prealbumin, transferrin and Hb in the experimental group were (38. 6 ±2. 0) g/ L, (281 ±33) mg/ L, (2. 5 ±0. 9) g/ L and (111 ±20) g/ L before nutritional support therapy and (38.2 ±1. 9) g/ L, (277 ± 16) mg/ L, (2.3 ± 1.1) g/ L and (112 ± 37) g/ L at postoperative day 8, respectivley. The Alb, prealbumin, transferrin and Hb in the control group were (38.3 ± 2. 4) g/ L, (287 ±34) mg/ L, (2. 4 ±1.1) g/ L and (107 ±21) g/ L before nutritional support therapy and (30.3 ± 2.3) g/ L, (190 ± 41) mg/ L, (1.6 ± 0.3) g/ L and (93 ± 22) g/ L at postoperative day 8, respectivley. There were significant differences in the nutritional indexes at postoperative day 8 between the 2 groups (F =174.042, 95.637, 9.529, 4.919, P <0.05) . The time to anal exsufflation in the experimental group were (52 ± 11) hours, which was significantly different from (70 ±12) hours in the control group (t =-5.176, P <0.05) . The incidence of complications was 15.4% (4/ 26) in the experimental group, which was significantly different from 58. 3% (14/ 24) in the control group (χ2 =6.460, P <0.05) . Patients with complications in the 2 groups were cured by anti-infective or symptomatic treatment. The duration of hospital stay was (9 ±3) days in the experimental group and (12 ±4) days in the control group, with a significant difference between the 2 groups (t =-2.912, P <0.05) . Conclusion TNA can improve the nutritional status of patients after radical gastrectomy in a short time. It could help patients to get through the perioperative period smoothly, and enhance the postoperative recovery. Key words: Gastric neoplasms; Nutritional support therapy, parenteral; Perioperative period

Laminoplasty using the Posterior Midline Approach in the Treatment of C1-2 Spinal Tumors.

This study aimed to investigate the method and efficacy of vertebral reconstruction using the posterior midline approach (PMA) in the treatment of C1-2 spinal tumors. Twenty-seven patients with C1-2 spinal tumors from the Affiliated Hospital of Luzhou Medical College, who underwent microsurgical tumor resection through an occipitocervical PMA and spinal reset-reconstruction from January 2007 to December 2013, were enrolled in the study. The clinical data and results of these patients were analyzed and summarized. All patients underwent a successful complete tumor resection, with no operative deaths. The postoperative pathological diagnoses were schwannoma, neurofibroma, and meningioma in 21, 1, and 5 cases, respectively. The follow-up period was 4-48 months. Postoperatively, 1 patient was independent in daily activities, and 26 patients were able to live and work normally. No significant change was found between preoperative and postoperative MRI sequences of the cervical spine, and no cervical instability and tumor recurrence had occurred. PMA is suitable as the preferred approach for resection of C1-2 spinal tumors, and the vertebral reconstruction maintains spinal stability.

Application of improved flushing method in anastomotic leakage after low anterior resection for rectal cancer

To investigate clinical effect of improved flushing method applied in anastomotic leakage (AL) aft er low anterior resection for rectal cancer. We performed a retrospective study to analyze the therapeutic eff ect for 39 AL from 438 patients who received Dixon surgery in the Affiliated Hospital of Luzhou Medical College. Among them, 23 patients received the traditional flushing method (the traditional group) and 16 received the improved flushing method (the improved group). Th e rate of AL was 8.9% (39/438). All patients were cured with non-operative treatment. Th e cleanliness degree of the surrounding skin of drainage tube in the improved group was higher than that in the traditional group. Th e duration of average hospitalization and extubation of drainage tube was shorter in the improved group than that in the traditional group (P<0.05). Compared with the traditional methods, the improved one is better for the AL patients. Both the patients themselves or their family members can chose it. The improved flushing method is worth to be spread in clinic.

泸州医学院附属医院2009-2012年口服抗糖尿病药物应用分析

泸州医学院附属医院2009-2012年口服抗糖尿病药物应用分析

Protein kinase C-beta/the 66-kDa isoform of the growth factor adaptor Shc oxidative stress pathway in mediating the production of the reactive oxygen species in premature infants exposed to hyperoxia

Objective To study the possible mitochondria oxidative stress signal transduction pathway, which probably mediates the production of reactive oxygen species in premature infants exposed to hyperoxia. Methods Hyperoxia group: 20 cases of premature infants whose gestational age was below 34 weeks were diagnosed as respiratory failure(treated with exposed to over 400 mL/L oxygen 48-69 h) in Department of Neonatology, the Affiliated Hospital of Luzhou Medical College, from Jul.to Dec.2012.Control group: 20 cases of premature infants with the same gestatio-nal age were diagnosed as no respiratory failure(exposed to air over 48 h in the same room simultaneously). The expression of protein kinase C-beta(PKCβ)/the 66-kDa isoform of the growth factor adaptor Shc(p66Shc)/prolyl isomerase1(Pin1)/phosphorylated the 66-kDa isoform of the growth factor adaptor Shc-Ser36(p66Shc-Ser36) were detected by immunohistochemistry and the production of mitochondrial reactive oxygen was detected by fluorescence microscope in the peripheral blood mononuclear cell of hyperoxia group and control group. Results Compared with the control group, the expressions of PKCβ, p66Shc, Pin1, phosphorylated p66Shc-Ser36 and the production of reactive oxygen in hype-roxia group were significantly increased(t=21.139, 5.592, 7.476, 16.895, 10.586, all P<0.001). There were positive correlations between the production of reactive oxygen and the expressions of PKCβ, p66Shc, Pin1, phosphorylated p66Shc-Ser36 in hyperoxia group(r=0.893, 0.795, 0.681, 0.663, all P<0.001). Conclusion PKCβ/p66Shc oxidative stress pathway might mediate the production of reactive oxygen species in premature infants exposed to hyperoxia. Key words: Protein kinase C-beta/the 66-kDa isoform of the growth factor adaptor Shc; Hyperoxia; Reactive oxygen species; Infant, premature

GW24-e1858 The expression of SKCa channel in human mesenteric artery from Chinese patients with hypertension

ObjectivesThe small conductance calcium-activated potassium (SKCa) channel is a key factor in the regulation of vessel tone and blood pressure. It has been proposed that activation of endothelial SK3 channel...

Expression of serum connective tissue growth factor in children with congenital heart disease and pulmonary artery hypertension and its significance

Objective To investigate the expression of serum connective tissue growth factor(CTGF) in congenital heart disease(CHD) with pulmonary artery hypertension(PAH) and its significance. Methods Fifty children with CHD in the Affiliated Hospital of Luzhou Medical College from May 2009 to Feb.2012 were selected in the study.Pulmonary artery systolic pressure(PASP) were measured by right heart catheterization.They were divided into 3 groups according to PASP levels as follows: normal PAH group(PASP<3.99 kPa, n=21), mild PAH group(3.99 kPa≤PASP<6.65 kPa, n=17), moderate or severe PAH group(PASP≥6.65 kPa, n=12); serum CTGF and transforming growth factor-β1(TGF-β1) were detected by using double-antibody sandwich enzyme linked immunosorbent assay.The relationship among the 3 groups and their PASP were analyzed. Results In comparison to normal PAH group, serum CTGF and TGF-β1 were significantly elevated in mild PAH group and moderate or severe PAH group(all P<0.01); compared with mild PAH group, serum CTGF and TGF-β1 were higher in moderate or severe PAH group(all P<0.01); CTGF levels were positive correlated with PASP both in mild PAH group and moderate or severe PAH group(r=0.828, 0.849, all P<0.01); there were positive correlations between CTGF and TGF-β1 level in mild PAH group and moderate or severe PAH group(r=0.714, 0.713, all P<0.01); there were positive correlations between TGF-β1 and PASP in mild PAH group and moderate or severe PAH group(r=0.623, 0.639, all P<0.05). Conclusions Serum CTGF levels are elevated in children with CHD and PAH, which may be an important factor for the formation and development of CHD with PAH. Key words: Connective tissue growth factor; Heart defect, congenital; Transforming growth factor-β; Hypertension, pulmonary

Application of oral psychotropic drugs in the Affiliated Hospital of Luzhou Medical College from 2009 to 2011

Application of oral psychotropic drugs in the Affiliated Hospital of Luzhou Medical College from 2009 to 2011

Antidepressant Effect of the First Electroconvulsive Therapy With Ketamine and/or Propofol

Luzhou Medical College Luzhou, China [email protected] The authors declare no conflict of interest.

Medical colleges summary of biomedical engineering profession&amp;lt;br&amp;gt;—Luzhou Medical College in the perspective of biomedical engineering profession&amp;lt;br&amp;gt;

This paper expounds professional characteristics of biomedical engineering in our school, and analyses some problems lying in it, emphatically discusses advantages and the problems combining biomedical engineering with the medical courses in order to offer targeted solutions. It summarizes the results and problems so as to provide reference value to a new major.

Assumption of Modular Courses in the Surface Electromyography Courses

 Abstract—The construction of courses should be highlighted for application oriented universities, while the traditional professional training system should be less concentrated. Based on the quality requirements of different professions, universities should establish a corresponding curriculum system and the market to satisfy students training to the maximum, and realize further the matching between graduates and their future jobs. Meanwhile, effective protection and incentive measures should be adopted for universities to ensure its openness and smooth operation of the courses market. In this paper, taking the basic content in Surface EMG of rehabilitation of muscle function for an example, it shows the modular courses between social sports (major) and rehabilitation medicine (major) of Luzhou Medical College.

关系型自我构念量表中文版的信效度检验&lt;br&gt;The Reliability and Validity of the Chinese Version of the Relational-Interdependent Self-Construal Scale

本文修订并检验了中文版关系型自我构念量表的信效度。参与本研究的有效被试包括来自泸州医学院的192名大学生和来自西南大学的237名大学生。研究发现新模型适配；内部一致性系数是0.80；关系型自我构念与独立我、互依我、短版独立我、短版互依我相关显著；与自信的相关是0.33；与16PF中的恃强性，兴奋性，有恒性，实验性，感情用事与安详机警性，心理健康人格因素，内向与外向性相关显著；性别差异显著(t = 5.47, p < 0.001)。结果表明该量表具有良好的信效度。 This study investigated the reliability and validity of the Chinese version of the Relational-Inter- dependent Self-Construal Scale (RISCS). The effective participants of this study included 192 University students from Lu Zhou Medical College and 237 University students from SouthwesternUniversity. New model were fit. Internal consistency reliability coefficient of the scale was 0.80. The relationship between RISCS and independence self, interdependent self, short version of independent self, interdependent self were related significantly. And then, the relationship between RISCS and self-confidence was calculated as 0.33. RISCS and dominance, liveliness, persistence, openness to change, emotional reasoning, mental health, in- troverted and outgoing of 16PF were significantly correlated. The difference of sex was significant (t = 5.47, p < 0.001). The results show that this scale is a reliable and valid instrument.

Methylprednisolone enhances the expression of Toll-like receptor 2 on macrophages in induced sputum from patients with chronic cough

To explore the relationship between airway inflammation and Toll-like receptor 2 (TLR2) expression on macrophages in induced sputum from chronic cough patients, and to observe the effect of methylprednisolone on this receptor. Eighty-six outpatients with chronic cough were enrolled in this study from the respiratory department in the Affiliated Hospital of Luzhou Medical College from September 2008 to March 2010. These diseases were diagnosed according to the guideline of Chinese Society of Respiratory Diseases in 2005, excluding severe liver, heart and kidney pathology and pregnant women. Among 86 cases, 31 were cough variant asthma (CVA), 14 upper airway cough syndrome (UACS), 16 eosinophilic bronchitis (EB) and 25 gastroesophageal reflux (GERC). Twenty healthy volunteers were included as controls. Induced sputum was collected and cell counts were analyzed. TLR2 expression on macrophages was determined by immunofluorescence staining. Cytokine levels were measured with enzyme linked immunosorbent assay (ELISA). (1) The total inflammatory cells [(3.4 ± 1.1) × 10(6)/L, (2.6 ± 0.6) × 10(6)/L, (2.6 ± 0.6) × 10(6)/L, (2.7 ± 0.5) × 10(6)/L] and neutrophils (0.52 ± 0.06, 0.58 ± 0.06, 0.52 ± 0.04, 0.54 ± 0.06) in induced sputum from CVA, UACS, EB and GERC were significantly increased (t value = 5.27, 4.09, 3.16, 3.92 and 3.62, 3.49, 4.82, 6.17 respectively, all P < 0.01), with decreased macrophages (0.35 ± 0.04, 0.40 ± 0.06, 0.38 ± 0.04, 0.43 ± 0.05) in comparison with the control group (t value = 4.76, 5.24, 4.57, 3.18, all P < 0.01). There was a significant increase in eosinophils (0.118 ± 0.054, 0.099 ± 0.034) in CVA and EB group (t value = 4.46, 3.87, 5.19 and 3.51, 4.06, 4.38 respectively, all P < 0.01). (2) The concentration of IL-8 [(7.0 ± 1.2) µg/L, (10.2 ± 3.1) µg/L, (6.3 ± 1.9) µg/L, (7.7 ± 2.5) µg/L] and TNF-α [(30 ± 11) ng/L, (46 ± 16) ng/L, (21 ± 5) ng/L, (33 ± 15) ng/L] from induced sputum in CVA, UACS, EB and GERC groups were significantly increased, compared with the control group(t value = 4.58, 3.67, 4.26, 5.39 and 5.16, 3.97, 4.59, 3.34 respectively, all P < 0.01). (3) The concentration of IL-8 was positively correlated with neutrophils in each group (R value = 0.628, 0.816, 0.769 and 0.733 respectively, all P < 0.05). The concentration of TNF-α was also positively correlated with neutrophils in each group (R value = 0.579, 0.865, 0.730 and 0.755 respectively, all P < 0.05). (4) The expression of TLR2 (56 ± 15, 38 ± 7, 65 ± 17, 27 ± 4) on macrophages in CVA, UACS, EB and GERC was significantly decreased. The expression of TLR2 (75 ± 17, 53 ± 16, 94 ± 30, 41 ± 5) on macrophages was enhanced by methylprednisolone. The expression of TLR2 on macrophages from induced sputum in CVA, UACS, EB and GERC was decreased, but could be enhanced by methylprednisolone, suggesting that TLR2 might play a regulatory role in airway inflammatory response.

Analysis of Factors Influencing Current Practice of Clinical Medicine Education

This study aims to analyze the factors that influence current practice of clinical medicine education through questionnaire among the undergraduates of 2003rd year (of graduation) in Luzhou Medical College who have just finished practice education. The sample comprises of 206 students. Then we get the first-hand data based on the personal experience of interns by analyzing the questionnaire, make clear the inherent reasons, thus take measures to achieve an etiological treatment and provide evidence for the direct formulation of educational reform measures so as to scientifically impelling the practice education reform.

Study on the Effects of Multimedia Monitoring System in Medical Teachers’ Microteaching Training

The study, investigated in Luzhou Medical College, aims to find out whether the use of Multimedia Monitoring System is more effective for medical teachers’ training on teaching ability compared to full-time teaching. Two groups of medical teachers have been involved in for the study. The first group has been allowed to practice the skills through micro-teaching with the aid of Multimedia Monitoring System. The second group practices their own skills through micro-teaching but without the aid of Multimedia Monitoring System. At the end of the study, we discover that the groups which have used the Multimedia Monitoring System have made more significant progress in the mastery of teaching skills.