Lupin Varieties Research Articles

Evaluation of the modern lupine varieties developed in the All-Russian Lupin Scientific Research Institute

The article presents the researches of the Institute within the frame of the breeding program for three cultivated lupine species: Lupinus albus L., Lupinus angustifolius L. and Lupinus luteus L. The authors analyze and compare the modern varieties of the cultivated lupine species included to the State Register of Breeding Achievements of Russia for the complex of economic-and-biological characters. The narrow-leafed lupine is the most early ripening; it allows to grow it in the most northern regions. The yellow lupine is the most adapted to light sandy loam soils. Its protein content in seeds is 45-50%. The white lupine has the highest grain productivity (5-6 t/ha) among grain legumes crops. Its grain quality is close to soya one (protein and oil content is 36-40% and 9-12% respectively).

Application of new forms of fertilizers to improve productivity and grain quality of white lupine

Substantiation and development of technology elements for obtaining a sustainable productivity of lupine grain is an important and urgent task for expanding the area under this grain crop. There have been considered the study results of the effect of fertilizers ‘Aquamix ST’, ‘Metaborat Kaliya’, ‘Tiaton’ on productivity and grain quality of white lupine. The study was carried out at the All-Russian Research Institute of Lupine – a branch of the FWRC FPA, in the soil and climatic conditions of the southwestern part of the Non-Chernozem zone in 2018-2019. The purpose of the current study was to estimate the efficiency of these innovative micro- and macrofertilizers and the methods of their application on a number of indicators that improve productivity and grain quality of white lupine. The application of fertilizers ‘Aquamix ST’ and ‘Metaborat Kaliya’ contributed to an increase of white lupine grain productivity on 37.5 and 35.4%. There has been determined protein increase from 5.0 to 5.8% in grain of the white lupine variety ‘Piligrim’ in all application variants of ‘Aquamix ST’. The use of ‘Aquamix ST’ and ‘Metaborat Kaliya’ in the vegetation period of plants reduced alkaloid content of lupine grain on 24.7-34.6%. Application of ‘Aquamix ST’ and ‘Metaborat Kaliya’ in white lupine budding stage increased carotene content in grain on 34.3 and 25.0%, respectively. The analysis of the data showed that the variant including pre-seeding treatment with ‘Aquamix ST’ and subsequent treatment in the budding stage of plants was characterized by the maximum amount of lysine and carotene. There was noted that the application of micro- and macrofertilizers resulted in an increase in feed units and digestible protein. Application of ‘Aquamix ST’, including pre-seeding treatment and two treatments in the vegetation period of plants provided the largest yield of digestible protein (1.26 t/ha), 6.0 t/h of feed units, and 54.8 GJ/ha of exchangeable energy. ‘Metaborat Kaliya’, used in the treatment of plants in their budding phase, showed almost the same results. The options that combined high productivity with high grain quality were more valuable according their feed value.

СЕМЕННАЯ ПРОДУКТИВНОСТЬ И АДАПТИВНОСТЬ СОРТОВ ЛЮПИНА БЕЛОГО В УСЛОВИЯХ ЦЕНТРАЛЬНО-ЧЕРНОЗЕМНОГО РЕГИОНА

In field experiments for four years (2019–2022) in the conditions of the northern part of the Central Chernozem region, the productivity components of different varieties of white lupin (Lupinus albus L.) were determined and presented in the article Dega, Delta, Start, Manovitsky, Timiryazevsky and Gana, the varieties of breeding of Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, were studied. Cultivation of high-yielding and high-protein varieties of white lupin increases the production of vegetable protein up to 15 c/ha (with a similar yield in grain crops, the protein harvest is four times lower). Thanks to nitrogen fixation, its production is possible without the cost of expensive nitrogen fertilizers, which is also beneficial in energy and environmental aspects. In addition, white lupin is able to assimilate phosphates that are difficult to dissolve. The influence of stress factors related to weather changes at different stages of vegetation on the variability of plant seed productivity parameters has been determined. The varieties of white lupine with determinant type of growth bred in Russian State Agrarian University – Moscow Timiryazev Agricultural Academy were characterized by a high level of adaptive potential to moisture deficiency (drought) and heat stress. The biological yield of the varieties was high, averaging 529, 573, 483, 564 g/m2 in 2019, 2020, 2021 and 2022, respectively. The share of the main stem in seed yield by year was 85, 74, 78, 88%. The coefficient of variation on the main shoot (V%) averaged 4.9% in varieties, 11.8% in years. On lateral shoots it was three times higher.

New sweet blue lupin, Lupinus angustifolius L. varieties (Sanabor and Vitabor) for Ethiopia

Though bitter white lupin is an ancient pulse crop to Ethiopia, sweet lupins are new to the country. Sanabor and Vitabor are recently introduced sweet blue lupin varieties to be used as multipurpose crop in the traditional lupin growing agro-ecologies of Ethiopia. These varieties were introduced from Germany and evaluated along with fourteen other varieties which have been tested in different locations of the country from 2009 to 2013. The varieties are registered at national level in 2014. The merits of the varieties are their low alkaloid content, high seed yield, resistance to anthracnose and fusarium, most importantly are palatable for livestock and can be used for human food. Thus, the varieties are recommended as multipurpose pulse crop for the traditional and new lupin growing areas.

Agrobiological evaluation of narrow-leaved lupin varieties in the conditions of the Middle Urals

Research and production introduction of the multipurpose use of narrow-leaved lupin varieties in fodder production in the region will increase the agricultural efficiency with developed dairy cattle breeding. The aim of the research was to study the adaptive properties of narrow-leaved lupin varieties in the Udmurt Republic, to analyze the efficiency of its cultivation. The studies were carried out in the southern agroclimatic region of the Udmurt Republic on gray forest soil. The arable soil layer was characterized by a humus content of 2.1-2.6%, mobile phosphorus - 100-101 mg/kg of soil, mobile potassium - 101-170 mg/kg of soil, and pH KCl from moderately acidic to almost neutral (5.0 -5.6). By the content of crude protein (32%) in grain, narrow-leaved lupin is the leader in the region among leguminous crops. During the research period, a relatively high yield of 1.36-4.95 t/ha was formed by Snezhet’ variety. Varieties Snezhet’ and Vector (S2d = 0.05…0.14) were distinguished by their high resistance to changes in agro-ecological conditions. The combination of ecological plasticity indicators (bi = 0.60) and phenotypic stability (S2d = 0.60) of Crystal variety indicates its high adaptive properties.

Использование люпиновой муки в хлебопечении

The results of a study of the possibility of using lupine flour produced from the seeds of the narrow-leaved lupine variety Deco 2 in the production of bakery products are presented. The possibility of using lupine flour in the amount of 5 % by weight of premium wheat flour has been established in order to improve the physicochemical and organoleptic indicators of bakery products. The possibility of adding 10–15 % lupine flour to enrich bread with biologically valuable nutrients without a noticeable deterioration in the quality of finished products is shown.

The first detection of Botryosphaeria sinensis on white lupine

Fungal strain MF KP-12.1, which is similar in morphological and cultural characteristics to Botryosphaeria fungi, was isolated from the seeds of white lupine variety Dega grown in Oryol region in 2018. Phylogenetic analysis of the large rRNA subunit (LSU), elongation factor-1α (TEF) and internal transcribed spacer (ITS) fragments was used to accurately identify the isolated strain as B. sinensis. When 2-week-old white lupine plants were treated under laboratory conditions with mycelial suspension of B. sinensis MF KP-12.1, plant damage reached 67 % on day 3, and 100 % plant death was observed on day 10. Pathogenicity of B. sinensis MF KP-12.1 was confirmed by re-isolation of the strain from damaged plant tissue. This is the first detection of B. sinensis on white lupine, which has not been previously reported as a host for this fungus. It is also the first detection of B. sinensis in Russia.

A multiplex PCR marker distinguishes between a series of four LanFTc1 alleles regulating flowering time in narrow‐leafed lupin (Lupinus angustifolius)

AbstractWe designed and validated a new multiplex PCR marker which discriminates between four insertion/deletion (INDEL) alleles in the 5′ regulatory region of a major flowering time gene in Lupinus angustifolius, LanFTc1. The four INDEL alleles were the wild‐type allele (ku) in variety ‘Geebung’ (G), a 1,208‐bp deletion allele in accession P22660 (P), a 1,423‐bp deletion allele (Ku) in variety ‘Tanjil’ (T) and a 5,162‐bp deletion allele (Jul) in variety ‘Krasnolistny’ (K). F2 PCR marker genotypes segregated as expected in populations K × G, K × T, P × G and P × T. Heritability of flowering times based on F2:3 parent:offspring correlation was high in K × G (0.81 ± 0.09), P × G (0.76 ± 0.10) and P × T (0.81 ± 0.11) but low in K × T (−0.04 ± 0.15) due to similar early‐flowering phenotypes produced by Ku and Jul. Progeny homozygous for the 1,208‐bp deletion allele resulted in a unique array of mid‐season phenology in the F2, F3 and F4 generations, which may improve agronomic adaptation. This multiplex PCR marker will improve the efficiency of introgression of the new INDELs into future lupin varieties.

Results of the study of the modern gene pool of narrow-leaved lupine

Narrow-leaved lupine (Lupinus angustifolius L.) is a valuable leguminous fodder crop adapted to a wide range of soil and climatic conditions. Modern fodder varieties of narrow-leaved lupine have a low content of alkaloids in the grain of 0.02-0.10%, a high protein content of 32.0-38.0% and a number of other nutrients valuable for fodder production. In the collection nursery 2018-2020 Under the conditions of a changed climate, forage varieties and specimens of two morphological groups were studied: branched with different degrees of blocking of lateral branching and spike-like. The results of the structural analysis of the gene pool for the main elements of productivity and its morphobiological features are presented. According to the productivity of plants in the indeterminate group, the varieties USN 53-236, Narrow-leaved 53-02 and Hybrid 1215 (7.7-6.1 g / plant) were distinguished; by weight 1000 pcs. seeds - varieties Belorozovy 144 and Crystal (136.4-120 g); by the number of seeds in a bean - Belorozovy 144 and Narrow-leaved 53-02 and the Belarusian cultivar Alliance (4.1-4.2 pcs.). In the group of spike-like forms, the cultivar Epigonal 1215 (3.2 g / plant) was distinguished by grain productivity, by the weight of 1000 seeds - the Belarusian cultivar Talent (102.2 g). As a result of the assessment of the collection material in terms of the height and weight of plants, a variety meter was identified that exceeded the control, variety Vityaz, by 15.0-27.7 cm: Belorozovy 144, SN 78-07 and Hybrid 1215. The growing season of the studied numbers varies in a wide range of 80- 101 days. The group of spike-shaped numbers is characterized by ultra-early maturity (71-75) days.

On the issue of protection of yellow lupine from anthracnose in the field

AnnotationOver the years of many years of research in the field, it was found that the creation of completely anthracnose-resistant varieties of yellow lupine (Lupinus Luteus L) is not possible. But at each stage of the pathological process, various defense mechanisms operate that determine the resistance or susceptibility of plants to diseases. There is a passive defense associated with morpho-biological features. A positive role in the control of anthracnose of early and mid-season forms with limited second-order shoots, fast-growing ones with anthocyanin coloration, xeromorphic leaf type, dark green color of vegetative organs, intensely colored seeds (2c, 4c, 2d), different corolla colors (numbers 52-87-2113 and 1477-1-17) and a flower (varieties Iputsky and Rodnik). Observations have shown that a waxy coating on a bean or a thick layer of hairs that helps water roll off, a more spreading bush that provides quick ventilation and moisture removal, reduces anthracnose infestation. It was found that the passive defense mechanism is largely due to the totality of morpho-biological characteristics and the nature of their interaction. Different infestation with anthracnose depending on the sowing density was shown: under conditions of sparse sowing (600 thousand seeds / ha), it averaged 4.6% during flowering and 8.0% at bean formation, and in a denser cenosis (1.2 mln. grain / ha), respectively, 18.7 and 62.0%. An active plant defense mechanism has been established and is being studied -hypersensitivity - this is an increased sensitivity of living cells of a plant - a host in places where a pathogen penetrates and its rapid death with the formation of necrosis. It was revealed that necrotic forms of yellow lupine are less affected by anthracnose during all phases of plant growth and development and, especially, during the formation of beans. At the site of penetration of the pathogen, an orange spot develops, around which a roller of dead tissue is formed, i.e., the pathogen’s access to living cells stops at the expense of which it feeds. The selected material with bean necrosis, without external sporulation, reduces the infectious load in the infected crops.

Dependence of productivity of white lupine seeds on the foliar dressing with macro- and micronutrient fertilizers

The paper presents the results of the studies on the determination of the effect of foliar dressings with macro- and micronutrient fertilizers on linear growth, the mass of air-dry matter of plants, leaf surface, the formation of a symbiotic apparatus and the yield of seeds of white lupine (Lupinualbus L.) in the conditions of black soils of the Central Black Earth region of the Russian Federation. The field experiments were carried out in 2018-2020 at the Department of Crop production, Breeding and Horticulture of Belgorod State Agrarian University. The object of the research is a high-intensity variety of white Degas lupine. The subject of the research is microfertilizer Aquamix-TV, potassium sulfate fertilizer (K2SO4), phosphate-potassium monophosphate fertilizer (KH2PO4). The weather during the years of the experiments was hot and dry. During the critical periods of the development of lupine plants, a moisture deficit was observed with the excess of heat. The soil cover of the experimental site was represented by a typical medium-thick low-humus heavy loamy black soil with a granulometric composition. The accounting area of the plot was 18 m2, the replication was fourfold and the placement was systematic. The experiment included six variants: control (without fertilizers), foliar dressing with Aquamix-TV micronutrient fertilizer, foliar dressing with potassium sulfate solution, foliar dressing with potassium monophosphate solution, foliar dressing with Aquamix-TV + potassium sulfate mixture and foliar dressing with Aquamix-TV + monophosphate mixture potassium. The analysis of the obtained data showed that the highest yield of seeds of white lupine variety Dega was obtained on variants with foliar dressing with micronutrient fertilizer Aquamix-TV in combination with potassium sulfate and monopotassium phosphate, which amounted to 3.52 and 3.51 t / ha, respectively, which is 0.52 and 0.51 t / ha or 17.3 and 17.1% more than the control variant.

The effect of pre-sowing lupin-oat mixture treatment with ozone and a biological product on the productivity formation and product quality

There have been presented the study results on the effect of pre-sowing seed ozonation of narrowleaf lupin (Lupinus angustifolius) and common oat on their germination energy, germination capacity, productivity and product quality. The study was carried out in the period from 2018 to 2020 in laboratory and field conditions of the Kostroma RIA, the branch of the FSBSI “Russian Potato Research Center named after A.G. Lorkh”. The purpose of the current study was to find out the efficiency of pre-sowing seed treatment with an ozone-air flow and treatment with a biological product for their germination energy, germination capacity, productivity and product quality. The objects of the study were the common oats variety ‘Yakov’ and the narrowleaf lupin variety ‘Belozerny 110’. The seeds of the lupin-oat mixture were ozonized at a dose of 5.0 mg/m3 for 15 minutes and 30 minutes using an RIOS-10(20)-0.5 ozonizer. For comparison, the seeds were treated with the biological product ‘Fitosporin-M’. Ultimately, there were identified the best parameters of the pre-sowing seed treatment of the lupin-oat mixture. On average, over the years of study, pre-sowing seed ozonation greatly improves the germination energy of agricultural crops on 4.8–8.3%, laboratory germination was improved on 3.0–5.0%. The largest green mass productivity of lupin-oat mixture during pre-sowing seed treatment for three years was 26.1 t/ha in the variant with an ozone dose of 5.0 mg/m3 for 15 minutes, which was more than control on 29.2%. In the variant with ozonation time increase to 30 minutes, the average productivity was 23.2 t/ha, which was 14.8% more than in the control. Pre-sowing seed ozonation contributed to dry matter increase on 14.2–19.0%; crude protein yield increased on 32.8–53.2%; crude protein percentage in grain increased on 14.1–16.8%; metabolizable energy increased on 3.7–5,1%; fodder units increased on 4.3–6.5% compared to control. When treating seeds with a biological product ‘Fitosporin-M’, the average productivity was 22.8 t/ha, which was on 12.9% more than control, an increase in dry matter was on 16.6%, an increase in crude protein yield was on 19.6%, an increase in crude protein percentage in grain was on 0.8%, an increase in metabolizable energy was on 1.9%, an increase in fodder units was on 1.0%.

Formation of seedlings of white lupin, Lupinus albus L. and blue lupin, Lupinus angustifolius L. in laboratory and field experiments

Modern varieties of white lupin can be an alternative to soy in overcoming vegetable protein deficiency: to produce or import? Unlike soybeans, lupin seeds do not contain trypsin inhibitors; they don’t need heat treatment to be used in feed. Lupin has a high attachment of beans. It is drought-resistant, adapted to environmental conditions with insufficient moisture. The formation of seedlings is a critical period in the ontogeny of lupin, preceding sprouting. At this time, parameters of seedlings such as the speed and vigor, density, distribution uniformity on the soil surface are determined. The objective is to determine the dynamic characteristics of seedlings of white and blue lupin seeds by alterations in the wet and dry weight of the seedling and its individual organs in laboratory and field experiments. In the laboratory experiments, seeds of white and blue lupins were germinated on sifted and calcined sand, moistened to 80% of the total moisture capacity at 20 oC. On the 4th and 7th days, the wet and dry weight per 100 seedlings, morphological alterations in the seedling bodies and their mass were determined, as well as the expenditure of seed reserve nutrients for the formation of seedlings was calculated. In field experiment, the same parameters of seedlings were determined, considering their laboratory germination (according to the number of viable seeds per 1 m row). The formation features of seedlings of white and blue lupins in laboratory and field experiments have been studied. The certain features influencing the parameters of seedlings in field during the critical sowing-seedlings” period have been determined.

Effects of bread making methods, lupin variety and gluten powder on the quality of bread enriched with high percentage of lupin flour

SummaryLupin is an economical source of protein, fibre and bioactive compounds, and to obtain these health and nutritional benefits lupin flour has been used in bread production. However, addition of more than 10% lupin flour markedly reduces bread quality mainly due to gluten dilution. The main aim of this research was to retain lupin bread quality enriched with higher percentages of lupin flour (20%) by addition of vital gluten powder (0%, 2%, 3.5% and 5%), investigating the effects of lupin variety (Lupinus albus and L. angustifolius) and two baking systems (rapid and sponge & dough). Impact on bread staling qualities was also determined through texture analysis of samples over a 72‐h storage period. Compared to lupin bread with nil gluten addition, significant improvements in loaf volume and crumb texture were observed with addition of gluten powder especially at 5% which increased loaf volume by an average of 20% across lupin sources and baking methods, and crumb softness by 30–50%. Differences were observed between the lupin flour sources. L. angustifolius had a reduced weakening effect when blended with the base flour compared with L. albus. The Sponge & Dough process was found to be more suitable to the inclusion of lupin flour than the rapid process.

Evaluation of protein extraction methods for in-depth proteome analysis of narrow-leafed lupin (Lupinus angustifolius) seeds

Lupin is slated as a potential contributor towards future food security. Lupin possesses several nutritional and nutraceutical attributes, many linked to seed proteins. For in-depth characterisation of the lupin proteome, liquid chromatography-tandem mass spectrometry was used to evaluate four protein extraction procedures. The proteomes of three narrow-leafed lupin were qualitatively evaluated using protein/peptide identifications and further quantitatively assessed by data-independent proteome measurement. Each extraction buffer led to unique protein identifications; altogether yielding 2,760 protein identifications from lupin varieties. The analysis of protein abundance data highlighted distinct differences between Tris-HCl and urea extracted proteomes, while also revealing variation amongst the cultivar proteomes with the wild accession (P27255) distinctly different from the domesticated cultivars (Tanjil, Unicrop). The extraction buffer used influenced the proteome coverage, downstream functional annotation results and consequently the biological interpretation demonstrating the need to optimise and understand the impact of protein extraction conditions.

The testing results of the new white lupine varieties and samples in the forest-steppe conditions of the Central Blackearth region

The variety study of white lupine plants in the Belgorod region was carried out in field trials at the collection nursery of department of plant production, breeding and olericulture of the Belgorod State Agrarian University named after V.Ya. Gorin in 2018–2019. In the trial there were studied 4 varieties and 26 samples of white lupine of grain-fodder selection developed by the All-Russian Research Institute of Lupine, the variety ‘Michurinsky’ was taken as a standard one. Most of the samples in the trial significantly exceeded the standard variety in the value of seed productivity. In 2018 the highest yield of 4.86 t/ha was obtained from the sample ‘CH 40-15’, which was on 1.15 t/ha higher than that of the standard variety. A high yield from 4.19 to 4.82 t/ha was produced by the samples ‘СН 78-16’, ‘СН 20-13’, ‘СН 15-13’ and ‘СН 816-09’, which significantly exceeded the standard variety on 0.48–0.61 t/ha. Under the conditions of the year of 2019, a significant productivity increase (from 0.24 to 2.1 t/ha) in comparison with the standard variety was produced by 19 samples and two varieties ‘Pilgrim’ and ‘Timiryazevsky’. The largest productivity in the trial (5.26 and 5.24 t / ha) was produced by the samples ‘CH 54-08’ and ‘CH 12-13’. The yield increase of the samples ‘СН 77-17’, ‘СН 816-09’, ‘СН 1735-10’, ‘СН 17-14’ was 1.35–1.57 t/ha in comparison with the standard variety. The highest mean yield in the trial for 2 years (4.40–4.47 t/ha) was formed by the samples ‘СН 17-14’ and ‘СН 816-09’, their increase was 0.96–1.03 t/ha or 27.9–29.9% in comparison with the standard variety. The samples ‘СН 12-13’ and ‘СН 54-08’ showed the highest coefficient of adaptability in the trial at the level of 1.36–1.38. The most of the studied samples and the variety ‘Alyi Parus’ possessed the coefficient of adaptability >1, which characterizes them as highly adaptable to the arid conditions of the region. The most varieties and samples exceeded the standard value of the trait ‘seed weight per plant’ which is 4 g. The value of the trait ‘1000-seed weight’ in the trial varied from 234.8 g (the sample ‘CH 35-13’) to 302.6 g (the sample ‘CH 25-11’) in comparison with 264 g (the standard variety).

Kinetics of solid-state fermentation of lupin with Rhizophus oligosporus based on nitrogen compounds balance

Solid-state fermentation could be a potentially effective method for improving the nutritional value of legumes. The objective of this study was to explore this technology in Lupinus mutabilis, a species of lupin, using a strain of Rhizopus oligosporus mold for solid-state fermentation. Three L. mutabilis varieties (INIAP-450, INIAP-451, and Criollo) were evaluated, including the effect of the grain status (whole or ground) and integument or husk (presence or absence). Kinetic parameters of the fermentation process were studied to define the specific speed of nitrogen formation and the digestibility and nutritional quality of the resulting protein. After incubation at 28 °C for 96 h, the titratable acidity increased, the pH decreased, and the total nitrogen of the whole grain with integument reached 110 g kg−1 for the Criollo variety. The ground INIAP-450 grain without integument had the highest soluble nitrogen content, and nitrogen formation occurred at a specific rate of 0.006 h−1. The apparent digestibility of the protein in this variety increased from 82 to 96% in debittered grain, the concentration of the first limiting amino acids (methionine + cysteine) increased from 20.8 to 27.9 mg g−1 crude protein, and the protein digestibility-corrected amino acid score increased from 68.3 to 89.3%. Overall, the results confirmed the benefits of fermentation in improving the content and quality of protein in the three lupin varieties.

Yield and Nutritional Quality of Sweet Lupine (Lupinus angustifolius) Grown in Midaltitudes of Lemo District, Hadiya Zone, Southern Ethiopia

The study was conducted to determine the yield and nutritional quality of sweet lupine (Lupinus angustifolius.) grown in midaltitude of Lemo District, Southern Ethiopia. The yield and nutritive value of sweet lupine in terms of quantity and quality was conducted using a factorial experiment arranged in a randomized complete block design (RCBD) with three replications. The treatments for the study were two sweet lupine varieties (Vitabore and Sanabore), two locations (Upper Gana and Jewe Kebeles, and six levels of planting spacing: 30 cm × 7 cm (S1), 40 cm × 7 cm (S2), 30 cm × 15 cm (S3), 40 cm × 15 cm (S4), 30 cm × 20 cm (S5), and 40 cm × 20 cm (S6)). The yield, chemical composition, and digestibility among parameters were studied. Sweet lupine varieties in Upper Gana Kebele gave the highest green forage yield (39.58 t/ha) and forage dry matter (4.84 t/ha) at 30 cm × 7 cm planting spacing, respectively. Seed yield (SYD) (t/ha) was highly affected ( P < 0.01 ) by location. The maximum seed (2.98 t/ha) yield was observed in Upper Gana Kebele with the minimum (2.15 t/ha) at Jewe Kebele. The forage in Jewe Kebele gave the highest organic matter (OM) (87.01%) and acid detergent fiber (ADF) (37.50%) content at a stage of 100% flowering. Sweet lupine forage in Upper Gana Kebele gave the highest crude protein (CP) content (23.11%) while the highest forage CP content was recorded at a planting space of 40 cm × 20 cm (23.67%). Sweet lupine forage gave the maximum in vitro organic matter digestibility (IVOMD) (69.10%) at a spacing of 40 cm × 20 cm in Upper Gana. The highest CP (29.11%) content and IVOMD (80.49%) of seed were recorded in Upper Gana Kebele. The overall result of this study suggested that green forage yield and forage dry matter yield are affected by location, planting spacing, and stage of flowering, whereas the chemical composition of sweet lupine forage was affected by location and variety interaction (dry matter and acid detergent fiber), location and stage of flowering interaction (OM, ADF and total ash), location (CP, metabolizable energy (ME), and IVOMD), planting spacing (CP and IVOMD), and stage of flowering (CP and ME). On the other hand, sweet lupine seed yield, seed CP, and IVOMD were affected by location. The large differences in yield and nutritive values observed among sweet lupine varieties, growth environment, planting spacing, and their interactions entail consideration of these factors for appropriate utilization of sweet lupine as a feed resource for livestock.

Effects of Blending Ratio and Lupine Variety on the Functional Properties of Composite Flours and Sensory Evaluations of Tef-Lupine Injera

This study was conducted to investigate the effect of lupine flour on functional properties and sensory acceptability of tef-lupine blended injera. Injera is a staple food for Ethiopian and it is fermented, sour leavened pancake-like bread made from blending of different cereals like tef, barley, sorghum, maize and wheat. Besides, there are limited studies on formulating of injera from composite flour with legumes (lupine). The effect of two factors two lupine varieties (Australian sweet lupine and Dibettered lupine seed) and blending ratios (0, 2.5, 5, 7.5, 10, 15, 17.5 and 20). Maximum and minimum levels of independent variables were first investigated by doing a preliminary analysis and founded that tef from up to80-100% and lupines from up to 0-20%. Response surface methodology was applied to find the formulations and predictive model. Sensory acceptance of tef-lupine injera was affected by interaction of varieties and blending ratios. Oil absorption capacity and swelling power properties of composite flour decrease as blending ratio of lupines increased and water absorption and foaming capacity increased as blending ratio of lupines increases for both varieties. As the sensory acceptability scores data indicated for both lupine varieties blended with tef for the production of injeras of up to 15% lupines almost all sensory attributes showed higher scores without significantly different among them but after 15% lupine addition there were observed drop of the sensory acceptability scores. In a 7 point hedonic scale, the composite sample tef injeras with 10% dibettered lupine seed variety addition had the highest scores of 6.09, 6.22, 6.09 and 6.18 in eye size, aroma, rollability and overall acceptability respectively. The L* value and number of eye by injera eye software were 72.77 to 79.84 and 14220.43 to 18929.33, respectively. The L values of blended injera increased as lupine proportion were increased, but the number of injeras eyes decreased. Keywords: Dibettered lupine seed, Composite injera, Response surface methodology DOI: 10.7176/FSQM/105-02 Publication date: February 28 th 2021

Status and prospects of breeding of cultivated species of Lupin in Russia

The article describes the types of white lupine (Lupinus albus L.), narrow-leaved lupine (Lupinus angustifolius L.) and yellow lupine (Lupinus luteus L.) cultivated in Russia. Easn of them has its own biological snaracteristics, differs in the length of the growing season, grain quality, yield, adapted to certain soil and climatic conditions and does not exclude one another. Among the cultivated species, white lupine has the highest yield potential (6-7 t / ha), the grain quality is close to soy and exceeds it in productivity and protein collection per unit area. Selection of these species is carried out in the all-Russian researsn Institute of Lupin. The directions of selection and the gene pool for their implementation are shown. The article describes some varieties included in the State register of breeding asnievements of the Russian Federation. The world collection of VIR, as well as its own hybrid and mutant material, is widely used as the source material for the selected traits. The introduction of new varieties of white, yellow and narrow-leaved lupine into the Russian feed production systems will increase the production of sneap, environmentally friendly feed protein, reduce the import of expensive genetically modified soy protein components, and increase the efficiency of domestic livestock production.