Lupin Seeds Research Articles

The effects of dry heat and steam on germination of dry and imbibed seeds of the invasive garden lupine (Lupinus polyphyllus Lindl.)

Abstract Regularly mown road verges are an important habitat for conservation of grassland vegetation. Disturbance and movement of seed-contaminated soil during road construction and maintenance makes road verges susceptible to the establishment of invasive alien plants such as garden lupine (Lupinus polyphyllus Lindl.). To combat spread of L. polyphyllus via seeds, we tested methods for seed destruction using heat. This study aimed at developing heat eradication methods for dry and imbibed L. polyphyllus seeds applying dry heat (88, 93, 98, 103 C at 1, 3, 5, 10 min) in a laboratory, steam (85, 90, 95 C at 3, 5, 10 min) in a test-box steaming device and (97 C at 10-17 min; dry seeds only) in a stationary soil-steaming machine (S30). In order to speed up water absorption and post-treatment germination, the imbibed seeds were manually scarified before the heat treatment and the dry seeds afterwards. Additionally, germination of two different age seed batches was tested applying dry heat (88, 98 C at 3, 5 min). The results showed that steam treatments inhibited seed germination more than dry heat in both dry and imbibed seeds. Germination dropped to < 5% when steamed at ≥ 90 C or dry-heated at > 100 C. Seed germination decreased with higher temperatures and longer exposure times. Imbibed seeds exhibited lower germination compared to dry seeds for dry and steam heat. Approximately 0.5% of dry seeds germinated when steamed using S30. 2022-collected seeds were less sensitive to dry heat than seeds from 2020. In conclusion, hot steam is more effective in reducing L. polyphyllus seed germination than dry heat. Thus, to successfully eradicate L. polyphyllus seeds in soil masses, we recommend steaming them at 97 C for at least 10 minutes.

Rapid assessment of the main economic value indicators in lupine flour samples using infrared spectroscopy

Background. A calibration model has been developed for rapid assessment of economic value indicators (protein, oil, and quinolizidine alkaloid contents) in the seeds of narrowleaf lupine accessions from VIR using near-infrared spectroscopy, with the help of which it is possible to decide on the further use of the accessions.Materials and methods. Biochemical quality indicators (protein, oil, and quinolizidine alkaloid content) were studied in the seeds of narrowleaf lupine (Lupinus angustifolius L.) grown in 2019 in the northwest of Russia. Calibration models for measuring protein, oil and alkaloids in lupine seeds (62 accessions) were developed using a MATRIX-I IR analyzer (Bruker Optics, Germany). To construct calibration models, we used the values obtained by chemical analysis methods accepted at VIR. The oil content in lupine seeds was assessed by the defatted dry residue technique in Soxhlet extractors, protein by the Kjeldahl method, and quinolizidine alkaloids by gas chromatography coupled with mass spectrometry. All indicators were recalculated on the dry-weight basis.Results and conclusion. Statistical significance of the developed models was verified according to the results of measuring the content of protein, oil and alkaloids in the seeds of the test batch. The protein and oil content data obtained using a calibration curve did not differ significantly from the results of chemical studies, in contrast to alkaloid indicators. Consequently, the developed calibration model for the MATRIX-I IR analyzer can be used for rapid assessment of protein and oil content in narrowleaf lupine flour samples, thus accelerating the process of obtaining data on the main economic value indicators. The analysis does not require reagents and is safe.

Proteome reorganization and amino acid metabolism during germination and seedling establishment in Lupinus albus.

During germination plants rely entirely on their seed storage compounds to provide energy and precursors for the synthesis of macromolecular structures until the seedling has emerged from the soil and photosynthesis can be established. Lupin seeds use proteins as their major storage compounds, accounting for up to 40% of the seed dry weight. Lupins are therefore a valuable complement to soy as a source of plant protein for human and animal nutrition. The aim of this study was to elucidate how storage protein metabolism is coordinated with other metabolic processes to meet the requirements of the growing seedling. In a quantitative approach, we analysed seedling growth, as well as alterations in biomass composition, the proteome, and metabolite profiles during germination and seedling establishment in Lupinus albus. The reallocation of nitrogen resources from seed storage proteins to functional seed proteins was mapped based on a manually curated functional protein annotation database. Although classified as a protein crop, Lupinus albus does not use amino acids as a primary substrate for energy metabolism during germination. However, fatty acid and amino acid metabolism may be integrated at the level of malate synthase to combine stored carbon from lipids and proteins into gluconeogenesis.

Development and Characterization of High-Fiber, Gluten-Free Pasta for Celiac Disease Patients

Celiac disease (CD) is a multi-organ complex autoimmune disorder triggered by a gluten-containing diet in genetically predisposed individuals. The only effective treatment for people with CD is strict, lifelong adherence to a gluten-free diet to reduce severe disease outcomes. Therefore, this study aimed to produce high-nutrition gluten-free pasta by substituting wheat flour with lupin flour, flaxseed flour, rice flour, and corn starch. For this purpose, six gluten-free pasta treatments (T1–T6) were produced with different flour compositions. In addition, inulin, xanthan gum, beta-glucan, and Moringa leaf powder in fixed amounts were added to all treatments. For the proximate analysis, color measurements and sensory evaluation were determined for all treatments. Proximate analysis of our results showed that substituting wheat flour with composite flour blends was satisfactory for producing nutritious pasta products without affecting their quality. Compared to the control group, T6 had a significant increase in fiber (4.68 ± 0.25 vs. 1.24 ± 0.28), lipid (21.99 ± 0.38 vs. 9.32 ± 0.25), protein (13.84 ± 0.30 vs. 13.45 ± 0.51), and ash content (1.65 ± 0.07 vs. 1.28 ± 0.06) of gluten-free pasta. However, the carbohydrate content decreased compared to the control treatment (46.10 ± 0.69 vs. 60.84 ± 0.75). The color measurement evaluation found a significant difference in the lightness (L*), redness (a*), and yellowness (b*) values between the control and all gluten-free pasta treatments. The sensory evaluation of the finished gluten-free pasta treatments and control sample indicated that the quality score for overall acceptability varied widely for different treatments due to individual preferences. Our study concluded that gluten-free pasta with high nutritional value from gluten-free flour is a good alternative product for celiac patients.

The Positive Impact of Lupine Meal in the Diets Intended for Poultry on Product Quality

Summary In a series of experiments conducted on broiler chickens, broiler ducks and laying hens, we showed that replacing soybean extract meal in nutrition with the Zulika variety of lupine seed meal had no negative impact on performance, improved health, especially in fattening chickens (natality) and above all, increased the quality of the acquired products (meat, egg yolk). The results indicated that increasing the nutrition quality of the products is given by a change in the composition of muscle fat or egg yolk fat. These changes are characterised by decreasing the content of saturated organic acids and on the contrary, by increasing the content of non‐saturated organic acids, which are a part of the fats. Nutrition based on lupine meal significantly increases the fat content, especially the content of n‐3 organic acids in the given products and this can be assessed as having a very positive impact. The stated changes in the quality of fat have to do with the unique content of oil contained in the lupine seeds. High‐quality protein product (seed) can be obtained by dehusking lupine seeds. This product is comparable to extracted soybean meal of average quality in terms of crude protein content. From a nutritional point of view, the very low content of fiber and fiber fractions, the higher fat content, and also higher brutto energy content can be positively evaluated in the lupine seed. When using dehusked lupine seeds and other lupine products in the feed mixtures prepared for monogastric animals, it is necessary to take into account the specific variety and its nutrient composition.

Evaluating the effect of replacing wheat flour with legume flour on ileal amino acid digestibility in healthy adults with an ileostomy

Protein-rich animal foods are highly digestible, high-quality sources or protein, whereas the protein quality of plant-based foods can vary considerably. Given the growing interest in alternative non-animal-based sources of protein, it is important to establish the protein digestibility of these new foods and protein concentrates which have important health implications especially for vulnerable groups who don’t consume sufficient dietary protein. The human ileostomy model is ideal for measuring protein digestibility as it enables protein digestion to be quantified independent of protein degradation in the large intestine. The aim of this study was to determine the protein digestibility and quality of a wheat-based food containing legume flours. This randomised, double-blinded, controlled cross-over intervention was conducted in 4 proctocolectomised adults with conventional and well-functioning permanent ileostomies. The study was conducted over 2 weeks and on each testing day, the participant consumed 2 test muffins (125 g each) or 2 protein-free cookies in the morning (breakfast and morning tea) followed by a standardised low-protein lunch and afternoon tea. Test muffins were made using a standard muffin recipe using wheat flour and for 2 of the test muffins 50% of the flour was substituted with soy or lupin flour. An indigestible marker, titanium dioxide was added to the muffins so that the completeness of muffin recovered in ileal digesta could be calculated. The digestible indispensable amino acid score (DIAAS) was determined by comparing concentrations of true ileal digestible indispensable amino acids to recommended amino acid requirements(1). Data was reported as mean ± SD and repeated measures ANOVA was used to compare means between treatment groups with significance reported at P < 0.05. Substituting 50% of wheat flour in muffins with soy or lupin flour doubled the protein content of muffins (soy 11.8 g/100g and lupin 10.6 g/100g) compared to muffins that only contained wheat flour (wheat 5.1 g/100g). However, substituting wheat with legume flour did not affect protein digestibility which was similar for all muffin types; wheat (76.8 ± 7.0%), soy (77.9 ± 7.4%) and lupin (81.6 ± 6.9%) (P = 0.181). The DIAAS values for all muffins were below 75% which is classified as the cut off for a good quality protein food. In conclusion, substitution of wheat-based muffins with soy and lupin flour increased the protein content of wheat-based muffins but protein digestibility and overall protein quality was similar.

Response of broiler chickens to raw, heat-treated and heat-treated dehulled sweet lupin seed (Lupins angustifolius) meals on feed intake, growth and carcass performances

The experiment was conducted to evaluate the effect of the partial substitution of soybean meal with raw and processed lupin seed (Lupinus angustifolius) meals on growth and carcass performances of Cobb 500 broiler chickens. Two hundred unsexed day-old chicks with similar body weight were randomly assigned to four treatment diets in completely randomized design with 5 replicates. The four dietary treatments diets were 0 % lupin seed meal (T1), 15 % raw lupin seed meal (RLS, T2), 15 % heat-treated dehulled lupin seed meal (HDLS, T3) and 15 % heat-treated lupin seed meal (HLS, T4). The CP content of RLS, HDLS, and HLS were 33.5, 37.1, and 35.4 %, respectively. The highest total and daily feed intake was observed in chickens reared in control diet (T1) while the lowest from T2 and T4 diets and differed (p<0.05) with the T1 diet. However, no significance effect was observed in feed conversion ratio among the treatment diets. Chickens fed with T1 diet had higher (p<0.05) final body weight, total and daily weight gains than those reared in T2 and T4 diets while these values were comparable to those fed on T3 diet. Similarly, higher (p<0.05) values were observed for slaughter weight, dressed carcass weight, drumsticks, keel bone meat, wings, neck and back and thorax in chickens fed with T1 diet than those of T2 and T4 diets; but these values did not differ with chickens fed on the T3 diet. The gizzard weight was similar between chickens fed with T1 and T3 diets while those on T2 and T4 diets had lower (p<0.05) values than those of T1 diet. The highest liver weight was observed in T1 diet while the lowest in T2 and differed (p<0.05). In conclusion it would be economically beneficial to replace 15 % of the soybean meal with heat-treated dehulled blue lupin seed meal in broilers ration under tropical environment

Perspective herbicides for post-sowing use in narrow-leafed lupin crops

Technical and economic efficiency of Pilot herbicide when applied on green leaf in narrow-leafed lupine crops was studied. The research was conducted in field and plot experiments according to the generally accepted methods in the southwestern part of the Non-Chernozem zone of Russia. The soil of the experimental field was gray forest light loamy in mechanical composition. The following application rates of the preparation were used: 1.5, 2.0 and 2.5 l/ha in the phase of appearance of two true leaves in lupine. The following plants showed high sensitivity even at the minimum application rate: cleavers (Galium aparine L.), wild buckwheat (Fallopia convolvulus L.), lamb's-quarters (Chenopodium album L.), one of the types of hemp nettle (Galeopsis sp. L.), shepherd's purse (Capsella bursa pastoris L. Medik), field violet (Viola arvensis Murr.), chickweed (Stellaria media L.). Application of GalactAlt graminicide (application rate 1.0 l/ha) after treatment of narrow-leafed lupine crops with Pilot herbicide promoted almost complete destruction of annual grass weeds. Application of Pilot herbicide had a favorable effect on nitrogen content in both dry aboveground mass and root mass of lupine. Reduction of weediness of crops when using Pilot contributed to the increase in lupine yield. In the variant with the application of 1.5 l/ha of the herbicide, the average yield of narrow-leafed lupine seeds exceeded the control by 0.53 t/ha in three years of the trials. The highest increase in seed yield of narrow-leafed lupine (0.96 t/ha) was observed in the variant that involved the application of herbicide Pilot at a concentration of 1.5 l/ha in the phase of one or two true leaves in lupine and herbicide GalactAlt at a concentration of 1.0 l/ha in the phase of lupine budding. Chemical analyses showed an increase in crude protein content in narrow-leafed lupine seeds by 2.3–4.2% when the crops were treated with Pilot herbicide compared to the control variant, where the treatment was not carried out.

The influence of different fertilizer standards on the quality of agricultural products in short rotation crop rotation

Goal. Study of the influence of different rates of fertilization of agricultural crops in short-rotational crop rotation on the quality of agricultural products and their yield on radioactively contaminated lands.
 Methods. Scientifically recommended agricultural crops for this region were selected for research in grain-row crop rotation: Oats — variety Chernihivskyi 27; Triticale — Aist Kharkiv variety; Corn — variety Kharkivska 195 MV; Fodder beets — Eckendorf yellow variety; Lupine is an Industrial variety. The chemical composition of feed was determined according to generally accepted methods. In plant products, the following were determined: crude protein — by total nitrogen using the classic Kjeldahl method; raw fiber — by acid-alkaline hydrolysis; raw ash — by burning samples in a muffle furnace at a temperature of 500—600°C; raw fat — by the Ruzhkosky method in a Soxhlet apparatus.
 Results. As a result of research in 2015—2017, it was established that the studied agricultural products in 2, 3 versions of fertilizer have a higher content of crude protein, crude fat, and crude fiber. The content of crude protein in lupine seeds was in the range of 25.54—31.01%, in oat grain — 10.37—12.21%, in triticale grain — 10.08—12.25%, in corn grain — 9.40—11.78%, in fodder beets — 2.03—2.65%. The raw ash content varied over the years of research from 2.68 to 2.86% in oat grain; from 2.74 to 3.01% in lupine seeds; from 2.19 to 2.41% in triticale grain; from 1.37 to 1.68% in beets and from 1.57 to 1.72% in corn grains. The crude fat content for the studied period was from 3.72 to 3.97% in oat grains; from 4.46 to 5.55% in lupine seeds; from 1.40 to 1.74% in triticale grain; from 0.12 to 0.16% in fodder beet and from 4.40 to 4.81% in corn grain. The content of crude fiber during the years of research was from 10.21 to 10.60% in oat grain; from 13.29 to 14.38% in lupine seeds; from 1.69 to 2.74% in triticale grain; from 0.93 to 1.05% in fodder beets and from 2.20 to 2.75% in corn grain. The average yield of oats over the years of research on fertilizer options was 27.12—35.01 t/ha, while it is monitored increase in yield by fertilizer options compared to the control by 15—46%. The yield of triticale was in the range of 39.75—48.97 t/ha, subject to an increase in yield in 2 and 3 fertilizer options by 3—7%. The yield of lupine over the years of research was 17.80—26.69 c/ha, while the yield of lupine in 2 and 3 fertilizer options was 4.8—17.2 higher than in the control. The grain yield of corn and fodder beet in 2 and 3 fertilizer variants also exceeded the control index. The yield of fodder beet was 297.09—394.74 t/ha. The yield of corn was in the range of 48.85—87.15 c/ha.
 Conclusions. It was established that the studied agricultural products in 2, 3 versions of fertilizer have a higher content of crude protein, crude fat, and crude fiber. The average yield of oats over the years of research on fertilizer options was 27.12—35.01 t/ha, the yield of triticale was in the range of 39.75—48.97 t/ha, the yield of lupine over the years of research was 17.80—26.69 t/ha, fodder beet yield was 297.09—394.74 t/ha, and corn yield was 48.85—87.15 t/ha.

Lupin as Ingredient in Durum Wheat Breadmaking: Physicochemical Properties of Flour Blends and Bread Quality.

The popularity of adding pulse flours to baked goods is growing rapidly due to their recognised health benefits. In this study, increasing amounts (3, 7, 10, and 15%) of white lupin flour (Lupinus albus L.) and of protein concentrate from narrow-leaved lupin (Lupinus angustifolius L.) were used as replacements for durum wheat semolina to prepare bread, and their effects on the physicochemical properties of the flour blends, as well as the technological and sensory qualities of bread, were evaluated. The addition of protein concentrate from narrow-leaved lupin and white lupin flour increased the water binding capacity and the leavening rate compared to pure semolina. A farinograph test indicated that the dough development time had a slight but significant tendency to increase with the addition of lupin flour and protein concentrate of narrow-leaved lupin, while had a negative effect on the stability of dough. The alveograph strength decreased (225, 108, and 76 × 10-4 J for dough made with semolina, 15% of protein concentrate from narrow-leaved lupin, and 15% of white lupin flour, respectively), whereas there was an upward trend in the P/L ratio. Compared to re-milled semolina, the samples with lupin flour and protein concentrate from narrow-leaved lupin had low amylase activity, with falling number values ranging from 439 s to 566 s. The addition of the two different lupin flours lowered the specific volumes of the breads (2.85, 2.39, and 1.93 cm3/g for bread made from semolina, from 15% of protein concentrate from narrow-leaved lupin, and from 15% of white lupin flour, respectively) and increased their hardness values (up to 21.34 N in the bread with 15% of protein concentrate from narrow-leaved lupin). The porosity of the loaves was diminished with the addition of the two lupin flours (range of 5-8). The sensory analysis showed that the addition of white lupin flour or protein concentrate from narrow-leaved lupin did not impart any unpleasant flavours or odours to the bread. To conclude, the use of lupin in breadmaking requires adjustments to strengthen the gluten network but does not require a deflavouring process.

Nutritional Properties of High Protein Pancakes Using Sorghum and Sweet Lupine

Nutritional Properties of High Protein Pancakes Using Sorghum and Sweet Lupine

Agrobiological evaluation of the varieties and cultivars of narrow-leafed fodder lupine

The reserve for increasing the production of high-protein fodder is the cultivation of leguminous crops, including narrow-leafed lupine (L. angustifolius L.). Protein content in the narrow-leafed lupine seeds is 33–35%, in the dry matter of green mass – 17–19%. It is one of the best forecrops for field crops, as it is able to accumulate 150-300 kg/ha of symbiotic nitrogen in its biomass. The research was conducted in the southwestern zone of the Central Region (Bryansk region). The soil of the fields is sod-podzolic loamy with an average fertility level, pH 5.1–5.6. The weather conditions did not quite meet the biological requirements of the crop. The results of the study of the varieties and cultivars of narrow-leafed lupin in the competitive variety trial 2020–2022 on grain and green mass yield, adaptability, alkaloid con tent and duration of the growing season are presented. The Vityaz variety included in the State Variety Register of the Russian Federation in six regions is taken as a standard. The varieties Bryansky kormovoy and Belorozovy 144 are released in all the regions of the country. Grain yield of different varieties and cultivars varied in the range of 1.59–3.74 t/ha during the years of study. The maximum value was obtained for Hybrid 1246 in 2020. SN 39-20 and Hybrid 1246 (2.86 and 2.76 t/ha) stood out in terms of average grain yield over the 3 years of the trial. The adaptability coefficient was 121 and 117%. The alkaloid content in the seeds of these varieties ranged from 0.010 to 0.046%, with an annual average of 0.028%. Less than 0.04% of alkaloids in the seeds were observed in the varieties SBS 56-15-1, Narrow-leafed 53-02 and Hybrid 1314. The duration of the growing season varied in the range of 78–95 days by year of the trial, the calendar maturity date was the end of July – the 2nd ten-day period of August. All of them are in the "very early" group (71–100 days). The minimum maturity date was observed in 2021 and the maximum in 2020. The varieties Belorozovy 144 and Hybrid 1314 have a longer (6–9 days) vegetation period compared to the standard variety Vityaz. They are characterized by high stature and intense lateral branching. The term of technological readiness for harvesting on green mass came in the first ten days of July. All lupine varieties and cultivars studied in the experiment exceeded the standard in green mass yield by 3.1-8.8 t/ha.

Liver Antioxidant Capacity and Steatosis in Laying Hens Exposed to Various Quantities of Lupin (Lupinus angustifolius) Seeds in the Diet.

Despite the many beneficial properties of legume plants, their use in diets for poultry is limited by the presence of antinutritional factors. The aim of the study was to determine the activity of DT-diaphorase, ethoxycoumarin O-deethylase, and catalase, and the concentration of malondialdehyde in liver tissue, as well as the activity of SOD and CAT in the serum of Hy-line Brown hens fed a diet supplemented with various doses of Lupinus angustifolius seeds. The results indicate that the use of large amounts of lupin in the diet resulted in an increase in MDA concentration in the liver and the lipid vacuolization of hepatocytes. A significant increase in DTD activity was observed in chickens receiving 15% lupin. Regardless of lupin dose, no increase in SOD activity was observed in chicken serum after 33 days of the experiment. From the 66th day of the experiment, an increase in catalase activity in the serum of laying hens was observed, while low activity of this enzyme was found in the liver. It can be concluded that the short-term use of lupin in the diet of laying hens does not affect the activity of antioxidant enzymes and, therefore, does not affect the oxidative-antioxidant balance of their body.

Evaluation of lupine seeds (Lupinus albus L.) neutral extract as a texture improver in low-fat yogurt production

Aqueous lupine seeds (Lupinus albus L.) extracts were evaluated as a natural fat substitute in low-fat yogurt production. Thus, the chemical composition, particle size, molecular weight, total phenolic (TPC), and total flavonoids (TFC) of the selected extract were estimated. Also, the antimicrobial activity and antioxidant capacity of selected extract were investigated. Yogurt with neutral lupine extract (NeLP) had the highest all sensorial attributes compared to other extracts. Also, the incorporation of NeLP during low-fat yogurt processing increased the solid content, and viscosity, as well as improved the textural profile and sensorial attributes without any negative effect on the yogurt's color. SEM micrographs of NeLP-yogurt microstructure showed a matrix characterized by large fused casein micelles clusters with comparatively lower porosity compared to control yogurt (without NeLP). The chemical composition of NeLP indicated that the major sugar constituents are glucose and galactose with different molar fractions. The molecular weight of NeLP is 460.5 kDa with a particle size of 1519.9 nm. Also, IC50 of NeLP is 0.589 mg/ml, while TPC and TFC are 7.17, and 0.0137 g/100 g sample, respectively. Hence, lupine neutral extract (0.25%) could be used as a fat replacer or texture improver ingredient in such low-fat yogurt which led to improved its characteristics without any negative defect during 7 days at 5 °C.

Performance Evaluation of a Commercial Real-Time PCR Method for the Detection of Lupin Traces in Food.

In accordance with U.S. FDA Foods Program Regulatory Science Steering Committee guidelines, with this study, we optimized and validated a commercial real-time PCR method for the detection of low amounts of lupin in four classes of food matrices: chocolate cookies, ragù, Olivier salad, and barley and rice flour. DNA extracted from blank (true negative) samples artificially contaminated with lupin (Lupinus albus) flour at 1000 ppm underwent dilutions with the DNA extracted from the true negative samples up to 0.5 ppm. The limit of detection for real-time PCR was 0.5 ppm in the complex matrices (range, Ct 26-34), making this a specific, robust, and rapid method for lupin allergen detection and labeling. Our validation data support the suitability of this commercially available real-time PCR method for this purpose.

Quantitation of Lupinus spp. Quinolizidine Alkaloids by qNMR and Accelerated Debittering with a Resin-Based Protocol.

Quinolizidine alkaloids (QAs) are toxic secondary metabolites of the Lupinus species, the presence of which limits the expansion of lupin beans consumption, despite their high protein content. Evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. However, quantitation of QAs is complicated by the fact that not all important alkaloids used for quantitation are commercially available. In this context, we developed a method for the simultaneous quantitation of eight major lupin alkaloids using quantitative NMR spectroscopy (qNMR). Quantitation and analysis were performed in 15 different seed extracts of 11 Lupinus spp. some of which belonged to the same species, with different geographical origins and time of harvest, as well as in all aerial parts of L. pilosus. The mature seeds of L. pilosus were found to be a uniquely rich source of multiflorine. Additionally, we developed a protocol using adsorption or ionic resins for easy, fast, and efficient debittering of the lupine seeds. The protocol was applied to L. albus, leading to a decrease of the time required for alkaloids removal as well as water consumption and to a method for QA isolation from the debittering wastewater.

Variability within L. albus and L. angustifolius Seeds in Dietary Fiber Components.

Lupin seeds have received increased attention due to their applications in the nutrition of humans and livestock. One of their special features is their high content of dietary fiber, which is influenced by the lupin species. No previous studies have focused on the variability in dietary fiber and its fractions within species so far. The aim of this study was to investigate the variability within L. albus and L. angustifolius (eight cultivars each) in the dietary fiber composition expressed as low-molecular-weight soluble dietary fiber (LMWSDF), soluble and insoluble non-cellulosic polysaccharides, cellulose, and Klason lignin. Additionally, we analyzed the proximate composition and the composition of amino acids and fatty acids. The results showed noticeable variability within both species not only in the total dietary fiber but also in all its fractions, especially in LMWSDF, cellulose, non-starch polysaccharides, and Klason lignin within L. angustifolius. This indicates that the cultivar choice should be based on the application for which it is used. Even though important nutrients, such as the most indispensable amino acids, are not highly variable within L. albus, dietary fiber variations can still have a marked influence on the nutritional value because of their influence on the digestibility of other nutrients.

Development and Characterization of High-Energy Protein Bars with Enhanced Antioxidant, Chemical, Nutritional, Physical, and Sensory Properties.

Protein-rich energy bars are known as an excellent nutritional supplement for athletes that help to build and repair connective tissues. The study is, therefore, aimed at developing high-protein bars using lupine seeds, wheat germ, and selected dried fruits including raisins, dates, apricots, and cranberries. Different formulations (F1, F2, F3, and F4) were performed at different ratios of ingredients to produce high-protein bars and compared them with a control bar made of whey-protein concentrate and oat flakes. For this purpose, a proximate analysis, total phenol content, total flavonoid content, DPPH radical scavenging activity, water content, nutritional, and sensory analysis was performed to evaluate the results. The proximate analysis of the produced protein bars showed a significantly higher protein content (22 ± 2) and total phenolic activity (57 ± 33) in formulation group 4 as compared to the other groups. Furthermore, the least water activity content was found in formulation group 1 (1 ± 0.0) when compared with the control group (1 ± 0.0). The results from the sensory evaluation revealed that T3 had the highest average scores in overall consumer acceptability. Our study found that total phenolic, flavonoid, and fiber content were significantly higher in the prepared protein bars indicating prospective health benefits when compared to the control group. Overall, the study demonstrates that high-protein bars using functional ingredients like dried fruit can provide enriched nutritionally valuable food options for consumers.

Evaluation of Phytase Impact on In Vitro Protein and Phosphorus Bioaccessibility of Two Lupin Species for Rainbow Trout (Oncorhynchus mykiss).

Legumes are an important source of protein, lipids, and other essential nutrients. As the demand for protein and lipids continues to surge on a global scale, there is a growing interest in incorporating legumes into aquafeeds. This shift is driven not only by the escalating growth of the aquaculture sector in recent years but also by the imperative to diminish the dependency on traditional resources like fishmeal (FM) and fish oil. Amongst legumes, different lupin species had been identified as a potential protein source to partially reduce the inclusion of FM in countries such as Australia, Chile, and the European Union. A comprehensive evaluation of their nutritional profiles, overall characteristics, and potential antinutritional factors is essential for informed utilization and the implementation of nutritional enhancement strategies. In pursuit of this goal, an in vitro gastrointestinal simulation system was devised to replicate the digestive conditions of rainbow trout (Oncorhynchus mykiss). The study focused on determining the bioaccessibility of protein and phosphorus within two sweet lupin varieties (alkaloids < 0.05) with high (Lupinus mutabilis) and low (Lupinus angustifolius) native phytic acid content evaluated as whole (W) or dehulled (D) seeds meals and the effect of a single dose of phytase (2,500 FTU/kg DM). Additionally, regular soybean meal (SBM) served as reference (10 treatments with 3 replicates). A 2,500 FTU/kg DM phytase dose increased the levels of PO4-3 released throughout the intestinal phase by 122.6% for L. mutatabilis W, 116.3% for L. mutatabilis D, 65.2% for L. angustifolius W, 59.0% for L. angustifolius D, and 91.8% for SBM compared to controls without phytase. The bioaccessibility of amino acids in varieties treated with phytase increased with respect to the control without phytase. L. mutabilis was found to be a potentially viable alternative as a good quality protein source for the manufacture of environmentally friendly aquafeeds.

Anticholinergic toxidrome: a rare consequence of lupin ingestion - a case report and literature review

Background: Lupin beans, a yellow legume seed from the Lupinus plant, are commonly used in Southern Europe, Latin America, and the Middle East. Despite their bitter taste, lupin beans are highly nutritious and rich in protein, making them popular snacks. The bitter taste is due to the presence of over 150 quinolizidine alkaloids, with Lupanine being the predominant alkaloid. If not properly debittered before ingestion, lupin toxicity and anticholinergic symptoms can occur. Case Presentation: A 49-year-old healthy Egyptian man presented with symptoms such as dry mouth, unsteadiness, constipation, and urinary retention for 6 hours. After investigation, it was discovered that he had consumed 400 g of bitter lupin beans within a 1-hour period, around 4-6 hours before the symptom onset. The patient was oriented, conscious, and alert, showing no signs of pain or distress. His vital signs were normal, and he had normal cardiovascular, pulmonary, gastrointestinal, and neurological examinations. His eye examination revealed fixed, dilated pupils bilaterally, blurry vision, and normal eye movement bilaterally. Laboratory tests were normal. The patient received Ringer&amp;apos;s lactate intravenously and was closely monitored in the emergency department. Over a 3-hour period, all symptoms and signs resolved with fluid management alone, and the patient was discharged home following his improvement. Conclusion: This report highlights the importance of history taking in diagnosing food-related diseases and emphasizes that physicians should consider lupin toxicity as a differential diagnosis in cases presenting with anticholinergic symptoms.