Lucilia Sericata Research Articles

Anti-inflammatory and wound healing potential of medicinal maggot excretions/secretions at the ocular surface

PurposeIn the skin, Lucilia sericata maggot excretions/secretions (ES) accelerate wound healing and limit inflammation. This study aimed to determine whether ES have similar beneficial effects at the ocular surface. MethodsHuman corneal epithelial cells (HCEC) were cultured with ES and cell viability was determined by the MTT assay. Additionally, mRNA expression of growth factors, antimicrobial peptides (AMPs) and cytokines was assessed by qPCR. ES ability to modulate TLR-induced IL-6 and IL-8 expression was determined by qPCR and ELISA. ES potential to promote corneal healing was evaluated in vitro by a migration assay in HCEC, and in vivo using a mouse model. ResultsES did not impair HCEC viability up to 25 μg/ml. Among the factors evaluated, only hBD-2 was upregulated (2.5-fold) by 1.5 μg/ml ES after 6 hrs (P = 0.04). In HCEC, ES reduced Poly I:C-induced IL-6 and IL-8 mRNA (P ≤ 0.001) and protein (P ≤ 0.0001) expression. A similar effect was observed with Flagellin (TLR5 agonist) but it was less robust for FSL-1 (TLR2/6 agonist) and Pam3CSK4 (TLR1/2 agonist). The greatest in vitro migration effect was observed with 6.2 μg/ml ES after 44 hrs where gap area compared to vehicle was 53.3 ± 3.7% vs. 72.6 ± 5.4% (P = 0.001). In the mouse model, the maximum healing effect was present with 1.5 μg/ml ES after 12 hrs with a wound area of 19.0 ± 2.7% vs. 60.1 ± 21.6% (P = 0.003) or 77% reduction of the wound area compared to the negative control. ConclusionsES significantly reduce in vitro TLR-induced production of inflammatory cytokines and promote corneal wound healing.

Chasing Flies: The Use of Wingbeat Frequency as a Communication Cue in Calyptrate Flies (Diptera: Calyptratae).

Simple SummarySome of the fastest and most agile flying insects are the calyptrate flies which include blow flies, flesh flies, and house flies. The current study compared the wingbeat frequency of four species, representing four genera of these flies, recorded in free flight and analyzed using sound editor and analysis software. Wingbeat frequency was found to differ between the four species and between sexes. Such findings provide insight into how flies are communicating and show promise for the use of wingbeat frequency as a novel methodology to identify fly specimens.The incidental sound produced by the oscillation of insect wings during flight provides an opportunity for species identification. Calyptrate flies include some of the fastest and most agile flying insects, capable of rapid changes in direction and the fast pursuit of conspecifics. This flight pattern makes the continuous and close recording of their wingbeat frequency difficult and limited to confined specimens. Advances in sound editor and analysis software, however, have made it possible to isolate low amplitude sounds using noise reduction and pitch detection algorithms. To explore differences in wingbeat frequency between genera and sex, 40 specimens of three-day old Sarcophaga crassipalpis, Lucilia sericata, Calliphora dubia, and Musca vetustissima were individually recorded in free flight in a temperature-controlled room. Results showed significant differences in wingbeat frequency between the four species and intersexual differences for each species. Discriminant analysis classifying the three carrion flies resulted in 77.5% classified correctly overall, with the correct classification of 82.5% of S. crassipalpis, 60% of C. dubia, and 90% of L. sericata, when both mean wingbeat frequency and sex were included. Intersexual differences were further demonstrated by male flies showing significantly higher variability than females in three of the species. These observed intergeneric and intersexual differences in wingbeat frequency start the discussion on the use of the metric as a communication signal by this taxon. The success of the methodology demonstrated differences at the genus level and encourages the recording of additional species and the use of wingbeat frequency as an identification tool for these flies.

Ovicidal Aroma Shields for Prevention of Blow Fly Strikes Caused by Lucilia sericata (Meigen), Diptera: Calliphoridae.

The blow fly, Lucilia sericata (Meigen) (Diptera: Culicidae) is a primary facultative ectoparasite controlled by insecticides that have environmental and safety concerns; therefore, its natural and safe control is crucial. L. sericata eggs were subjected to 400 μL of 5% of 24 materials of plant-borne origin. The number of hatched and unhatched eggs were counted 24 h postexposure. Stopmyasis® expressed the highest ovicidal effect, followed by cedarwood, orange, and tea tree oils. The mean ovicidal results were 46.09-95.24% grouped as Class A, which provided the lowest hatchability rates. Even though benzoin, vanillin, citronella, and camphor oils grouped as Class B provided moderate ovicidal effects (33.69-43.92%), their efficacy differed significantly from those of the treatments in Classes A and C containing the control group and vetiver, eucalyptus, Olbas®, neem, sunflower, oil blends, patchouli, frankincense, p-menthane-3,8 diol (PMD), lavender, peppermint, cinnamon, calry sag, myrtle, and silicone oil. According to our knowledge, most (19 out of 24) applied materials were used as ovicides against L. serricata for the first time, except orange, eucalyptus, patchouli, cinnamon oils, and six of them were applied as ovicides against pests for the first time. Stopmyasis containing Géraniol and PMD is the drug of choice as an ecofriendly product to prevent blow fly strikes through correct diagnosis and prompt treatment preventing a disastrous and destructive course of the disease and improving quality of life.

New Record of Lucilia sericata (Wiedemann) From Kargil Ladakh

Lucilia spp. also known as green bottle fly is well distributed, and it has forensic, medical and veterinary importance. In the present study, Lucilia sericata (Wiedemann, 1830) has been recorded from the Trans-Himalayan region of the cold arid desert Kargil Ladakh. Distribution over a period from April 2018 to March 2021 revealed that it is fairly distributed with maximum abundance being in midsummer July and August, with no activities during November to March.

A human tissue-based assay identifies a novel carrion blowfly strain for maggot debridement therapy

Maggot debridement therapy (MDT) is a form of therapeutic wound treatment in which live fly larvae are used intentionally to debride necrotic tissues. MDT has been widely used to treat chronic wounds in humans or animals, such as diabetic foot ulcers. Larvae of a carrion blowfly, Lucilia sericata (green bottle fly), debride wounds by consuming necrotic tissue and removing pathogenic bacteria, promoting effective wound healing. Most medical L. sericata strains were initially collected from natural environments using animal meat as bait and reared on artificial protein-rich media or ground meat. It remains to be examined which strain would be more appropriate for MDT, whereas any method for evaluating the fly’s therapeutic potential in humans has not been available. A feeding assay was developed using minced human tissues obtained from surgical waste. To establish L. sericata strains highly eligible for MDT, carrion fly larvae were collected from 45 corpses subjected to forensic autopsy (such as decomposed bodies). Four corpse-derived L. sericata strains were obtained and evaluated using the feeding assay. One strain showed that its feeding activity was 1.4 times higher than the control strain used in conventional MDT. The body length of the adult fly of the corpse-derived strain was longer than the control, which was consistent with the observation that its cell size was enlarged. The human tissue-based assay developed in this study accurately evaluated the ability of fly larvae to debride necrotic wounds. The L. sericata strain newly established from human corpses harboring high feeding activity may offer a clinically significant improvement in MDT.

Diversity and Abundance of Insects Pollinator of Chayote (Sechium edule (Jacq.) Swartz

Chayote plants (Sechium edule) with monoecious characters require a pollination process. The pollination process requires pollinating agents to increase its productivity, one of which is insects. This research aimed to determine the diversity and abundance of insects pollinator on chayote plants. Observation of diversity and abundance used a scan sampling method. Pollinator insects observations were carried out in 3 time periods, morning, afternoon, and evening on male and female flowers. We measured environmental parameters such as temperature, humidity, wind speed, and light intensity. Eight species of wild insects pollinated chayote flowers, Apis cerana, Apis dorsata, Lasioglossum leucozonium, Polistes sagittarius, Phimenes flavopictus, Campsomeriella annulata, Lucilia sericata, and Musca domestica. The insect pollinators community had moderate diversity (1.23), a relatively dynamic community (0.59), and moderate dominance (0.62), with A. cerana, which had been the dominant insect pollinator with a relative abundance of 61.63%. Musca domestica and L. sericata were (0,58%) the least dominant insect pollinator with a relative abundance of 0.58%. This research concludes that the insects pollinator of chayote has a moderate level of diversity, relatively dynamic community, and average dominance.

Reduced oviposition period promotes blowfly population extinction in Nicholson’s model

ABSTRACT Blowflies use open wounds or the accumulation of feces or urine in wool to lay their eggs. The larvae that emerge cause lesions in the host sheep, which can lead to death. They are found in Australia, New Zealand, and the United Kingdom. Nicholson’s model describes the population dynamics of the Australian blowfly (Lucilia Cuprina). It incorporates environmental variation. The extinction of these flies depends on the time to oviposition and the time between generations. The Lyapunov function, which is positive with a negative derivative, provides the condition for the stability of the equilibrium point: the oviposition period must be sufficiently short, because the shorter it is, the more it favors the extinction of the species. The zero solution is the only equilibrium point, synonymous with the extinction of the population. Another species of blowfly, Lucilia Sericata, also attacks sheep in Australia. Both blowflies are ectoparasites of warm-blooded vertebrates, particularly domestic sheep. These two blowflies are related to share same mitochondrial DNA sequences, although the two species are distinct. Presumably to avoid competition between them. the egg-laying time of each species does not occur at the same time of year: L. Sericata prefers warmer months, thus in summer, while L. Cuprina is mainly active in autumn. Laying of eggs in different months allows avoiding competition between these species. This also binds them together. A sufficiently small egg-laying delay then leads to the rapid extinction of both blowfly populations, provided they do not adapt.

Determination of the important insect fauna of forensic entomology in Kayseri province

The aim of this thesis is the determinations of insects species of forensic importance in Kayserı province. The experimental part of our study was carried out between September 2018 – May 2019; during the periods of winter, spring, autumn seasons in four different regions. In this study, fresh cow liver and chicken muscle were used as the experimental materials. The traps which allow the insects to enter easily but which prevent exit were used to collect insect species. As a result, 21 species were identified in four different study areas. Calliphora vicina (Robineau-Desvoidy, 1830) (Diptera: Calliphoridae), Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae), Chrysomya albiceps (Wiedemann, 1819) (Diptera: Calliphoridae), Muscina stabulans (Fallen, 1817) (Diptera: Muscidae), Musca autumnalis (De Geer,1776) (Diptera: Muscidae), were identified in fall season at all study areas. C. vicina was identified in winter season. C. vicina, Thricops sp. (Rondani, 1856) (Diptera: Muscidae), Fannia canicularis (Linnaeus, 1761) (Diptera: Fanniade), L. sericata, Dermestes frischii (Kugelann, 1792) (Coleoptera: Dermestidae), were identified in spring season at all study areas. C. vicina was seen from September to the end of May at all study areas. L. sericata was seen from September to the end of November and April to end of May at all study areas. Therefore, C. vicina and L. sericata can be used as an indicator species for determining the Postmortem Interval in Kayseri.

Diversity of flies (Diptera: Brachycera) in breeding farms in the Kabylia region (North-central Algeria), and identification of some myiaseginic species

Abstract. Ourrad O, Ahmed DSA, Sadou SA, Bouzrarf K, Belqat B. 2022. Diversity of flies (Diptera: Brachycera) in breeding farms in the Kabylia region (North-central Algeria), and identification of some myiaseginic species. Biodiversitas 23: 2276-2284. This work provides the first checklist of the flies (Insecta: Diptera) present in breeding farms located in the south Mediterranean region of Kabylia, Algeria. A new inventory of 26 species (631 individuals) of flies belonging to 15 genera and 8 families was presented from five sites with different altitudes (245 m - 1041 m) in the Kabylia region. Of them, eight species (Calliphora vicina, C. vomitoria, Lucilia sericata, L. ampullacea, Sarcophaga africa, S. carnaria, Musca domestica, and Fannia canicularis) are epidemiological, pathogenic agents of various animal and human myiasis. The fly fauna was found at varying frequencies in the five sites located at different altitudes. Besides, a negative correlation was observed between the altitude of the farms and fly distribution. The results of the PCA and FCA analysis offered better discrimination of the fly species according to the variation of the environmental parameters.

Cuticular hydrocarbons for the identification and geographic assignment of empty puparia of forensically important flies

Research in social insects has shown that hydrocarbons on their cuticle are species-specific. This has also been proven for Diptera and is a promising tool for identifying important fly taxa in Forensic Entomology. Sometimes the empty puparia, in which the metamorphosis to the adult fly has taken place, can be the most useful entomological evidence at the crime scene. However, so far, they are used with little profit in criminal investigations due to the difficulties of reliably discriminate among different species. We analysed the CHC chemical profiles of empty puparia from seven forensically important blow flies Calliphora vicina, Chrysomya albiceps, Lucilia caesar, Lucilia sericata, Lucilia silvarum, Protophormia terraenovae, Phormia regina and the flesh fly Sarcophaga caerulescens. The aim was to use their profiles for identification but also investigate geographical differences by comparing profiles of the same species (here: C. vicina and L. sericata) from different regions. The cuticular hydrocarbons were extracted with hexane and analysed using gas chromatography-mass spectrometry. Our results reveal distinguishing differences within the cuticular hydrocarbon profiles allowing for identification of all analysed species. There were also differences shown in the profiles of C. vicina from Germany, Spain, Norway and England, indicating that geographical locations can be determined from this chemical analysis. Differences in L. sericata, sampled from England and two locations in Germany, were less pronounced, but there was even some indication that it may be possible to distinguish populations within Germany that are about 70 km apart from one another.

Sterilization of Lucilia sericata (Diptera: Calliphoridae) Eggs for Maggot Debridement Therapy.

Maggot debridement therapy (MDT) is a therapy with the medical use of sterile fly larvae of certain species, particularly those within the Calliphoridae family including green bottle fly, Lucilia sericata (Meigan, Diptera: Calliphoridae), for treating chronically infected wounds and ulcers. Lucilia sericata flies were maintained under insectary conditions, and the eggs were sterilized using three treatments: hydrogen peroxide solutions, used as a hand disinfectant (Treatment 1-T1), hydrogen peroxide, surface disinfectant (Treatment 2-T2), and SaniHigene (Treatment 3-T3) and the control (without treatment). All three treatment caused the complete sterilization of eggs, and no bacterial colonies were found on the blood agar culture. The egg hatching rate after 72 h was much higher than after 24 h. Egg mortality in hydrogen peroxide solutions, T1 and T2, was 3-4% and less than in solution T3 (13%). Owing to less mortality and more sterility of the eggs, the aforementioned solutions are suggested to be appropriate for sterility in maggot therapy.

KERAGAMAN JENIS LALAT DAN EKTOPARASIT (JAMUR) PADA KAKI LALAT DI PASAR PETERONGAN KOTA SEMARANG

Flies are one of a mechanical vector that could spread a lot of disease, they also often get associated as a carriers of diseases such as cholera, typhoid, dysentery, diarrhea, and etc. The presence of flies itself in one place can be used as an indicator that the area was less hygienic. The purpose of this research was to find out the diversity of fungi and flies at the Peterongan Market, Semarang. This research was an explanatory study that was using a survey method and laboratory examination with cross sectional approach. The population of this study was all the flies that caught at the Peterongan Market, Semarang. The sampling was done by using accidental sampling at three different stalls on the ground floor of Peterongan Market. There was 25 flies that caught in this research, including of Musca domestica, Phaenicia sericata, and Sarcophaga. There was also several fungal spores that accidently carried by the flies on their feet just like Mucor, Rhizopus arrhizus (Rhizopus oryzae), Aspergillus fumigatus, Aspergillus flavus. Most common flies and fungal spores that found in this research was Musca domestica and Aspergillus.

Reviving hope by using of maggot debridement therapy in patients with diabetic foot ulcer: A case report study.

Introduction and importanceRefractory diabetic foot ulcers (DFUs) do not easily respond to standard therapeutic approaches and the prevention of DFU-related amputation is one of the most important aspects of treatment in patients with DFU.Case presentationThe present case report is 51-year old male patient with a history of 5-years type 2 diabetes who has had DFU on the first distal phalanx foot of his right foot with size of 2 × 2 cm. The patient was repeatedly hospitalized for receiving DFU treatment, he did not recover using routine wound treatment. The patient was transferred to our wound care team. After ten sessions (one session every 48 h) of maggot debridement therapy (MDT) using sterile Lucilia sericata, the patients' DFU had completely healed.Clinical discussionDFUs can change patient's quality of life and lead to infection, amputation, sepsis, and finally death. Thus, efficient therapeutic methods are necessary for management of DFUs.ConclusionThis case report was revealed that the maggot therapy is an affordable and highly efficacious treatment method to enhance the recuperation of DFUs. Therefore, it is recommended that wound care teams use this approach to speed up the healing process.

Spatio-temporal distribution and habitat preference of necrophagous Calliphoridae based on 160 real cases from Switzerland

Necrophagous blowflies (Diptera: Calliphoridae) are of great importance particularly during investigations of suspicious deaths. Many studies have analyzed the distribution of blowflies based on pig experiments and baited trapping; however, data from real case scenarios are rarely used. In this article, the distribution of blowflies found during investigations of 160 real cases during 1993–2007 in Switzerland is evaluated based on habitat, altitude, and season. Ten species of blowflies were present in 145 out of the 160 cases. The most common species was Calliphora vicina, which occurs throughout the year and was present in 69 % of all cases. Lucilia sericata, Calliphora vomitoria, and L. caesar were identified among the rest of the flies as species of great forensic importance mainly due to their distributional patterns. After a comparison with a similar dataset from Frankfurt, Germany, some surprising differences were determined and discussed. The biggest discrepancies between our dataset and the German dataset were in the occurrences of L. sericata (30 % vs. 86 %, respectively), Phormia regina (5 % vs. 43 %), and L. ampullacea (1 % vs. 45 %). The life-history strategies and intraspecific behavioral variability of blowflies remain understudied, although they can be essential for an unbiased approach during a death investigation. Further research and comparison of occurrence patterns across the area of distribution of blowflies are therefore needed and recommended.

Developmental niche construction in necrophagous larval societies: Feeding facilitation can offset the costs of low ambient temperature

Abstract This study explored the trade‐offs between thermal regulation and aggregation, two key factors impacting blow fly (Diptera: Calliphoridae) larvae development. Recent works have demonstrated that necrophagous maggots engage in developmental niche construction, which provides adaptive benefits. First, each species has a preferential temperature, at which larvae grow fast and efficiently. Second, larvae are attracted by each other and aggregate in large maggot masses. These groups modify the local environment and facilitate the exodigestion process (niche construction by perturbation). However, aggregation and relocation towards thermal preferendum are not always compatible under field conditions, forcing larvae to make choices. To test the developmental consequences of such trade‐offs, 40 or 80 Lucilia sericata larvae were placed on a thermal gradient (from 22 to 48°C) with or without a captive aggregate of 40 larvae located at 22°C, and their development speed, size and survival were measured. A previous study showed that in such situation the free larvae alone relocated at 33°C, while in the presence of captive larvae they gathered with the captive group at 22°C. In the present developmental study, we observed that such 22°C aggregated larvae actually grew as fast as if they were at 33°C. This result shows that niche construction, here resulting from larval gregarism and feeding facilitation, can compensate for the physiological costs of low ambient temperature. This finding confirms that aggregation of necrophagous Diptera larvae is an efficient adaptation to the carrion environmental constraints and highlights the adaptive value of developmental niche construction.

Flame impingement affects survival and development of Lucilia sericata (Diptera: Calliphoridae) on securely wrapped remains

Wrapping and burning are techniques to hide or destroy evidence on human remains. Both may influence the ability for forensically important arthropods, such as blow flies (Diptera: Calliphoridae), to colonize and develop to adulthood on remains. The combined effects of wrapping and burning a body on blow flies has not been examined in the literature, therefore this was examined using two experiments: (1) measuring the potential delays in oviposition due to wrapped remains and (2) the survival of blow fly larvae on wrapped bodies exposed to flame impingement. First, three domestic pig carcasses were wrapped in 100% polyester blankets and sealed at the end with duct tape, while three remained un-wrapped. After three days of exposure to natural colonization, blow flies delayed oviposition by at least 21 h on the wrapped remains. Second, six pig carcasses were colonized with 250 first instar Lucilia sericata (Meigen). Three of these carcasses were wrapped as described in the first experiment. Larvae were allowed three days of feeding and development, then the pigs were transferred to a rural, two-storey farmhouse, which was set ablaze. The local fire department suppressed the fire and removed the pigs. Compared to un-wrapped carcasses, wrapping increased survival of live larvae and recoverability of live and dead larvae. The blanket acted as an extra layer of protection, increasing maggot survival, however, live insect evidence collected and reared post-burn provided a less accurate minimum post-mortem interval estimate than maggots killed by the fire.

Mitochondrial and nuclear DNA-based identification of some forensically important calliphoridae (diptera) in Luoyang of China

Introduction: Calliphoridae plays a key role in forensic entomology research, which is one of the first insects to decompose animal carcasses.The mitochondrial cytochrome c oxidase subunit I and the ribosomal internal transcribed spacer 2 (ITS2) are among the most widely used molecular markers for insect taxonomic characterization. Aim: The aim of the study was to test the suitability of two genetic markers based on conducting the molecular identification of six necrophagous Calliphorid flies. Materials and Methods: Fourteen Calliphoridae flies were collected and classified with traditional morphological characteristics. The DNA of flies was extracted and the fragments of COI and ITS2 were amplified and sequenced. All the sequences were aligned and analyzed by MEGA 7 software for NCBI BLAST, nucleotide composition, intra- and inter-specific divergence calculation, and phylogenetic tree inference successively. Results: The results indicated that COI and ITS2 genes were robust in the identification of Calliphoridae at the species level and ITS2 gene sequence possessed a strong resolution power as it showed higher variation values between Lucilia sericata and Lucilia cuprina, Calliphora vomitoria and Triceratopyga calliphoroides, C.vomitoria and Aldrichina grahami, but inferior to COI for T. calliphoroides and A. grahami. Conclusions: Our results showed that combination of COI + ITS2 genes yields more accurate identification and diagnoses and better agreement with morphological data than the mitochondrial barcodes alone. As a supplementary method for morphological identification, we advocated for the combination of nuclear and mitochondrial gene approaches to address the taxonomy and phylogeny of forensic relevant flies, especially of closely related species and populations.

Comparison of common green bottle flies (Lucilia sericata Meigen) and stingless bees (Tetragonula laeviceps Smith) as pollinating agents for imported true shallot (Allium cepa L.) seed production

Comparison of common green bottle flies (Lucilia sericata Meigen) and stingless bees (Tetragonula laeviceps Smith) as pollinating agents for imported true shallot (Allium cepa L.) seed production

Assessment of nursing and midwifery students’ knowledge of treatment of diabetic foot syndrome using Lucilia servicata larvae

Background. Diabetes mellitus is one of the most common chronic diseases, and in recent years its prevalence has been systematically increasing. Untreated or ineffectively treated diabetes leads to the development of many complications. Among these that can significantly affect the quality of life is diabetic foot syndrome (DFS). Larvotherapy is a therapeutic method of treating wounds arising during the course of DFS. Aim of the study. The main objective of this study was to assess the level of knowledge of nursing and midwifery students on the treatment of DFS using Lucilia sericata larvae. Material and Methods. This study is a cross-sectional, observational study in which 202 nursing and midwifery students of Opole University were surveyed. The study was conducted online between June and November 2020, using a questionnaire developed by the authors. Results. The analysis showed considerable variation in the level of students' knowledge of treatment techniques for DFS. Those with the highest level of knowledge represented 39.15% (n=79), the average level of knowledge was represented by 28.2% (n=57), and the lowest level of knowledge was represented by 32.7% (n=66) of students. Regarding biosurgery in general, the highest level of knowledge about biosurgery was represented by 48.0% (n=97) of students. Nursing students had a higher level of knowledge than midwifery students regarding DFS treatment methods (p=0.001). There was no difference in the level of knowledge about biosurgery between nursing and midwifery students (p=0.503). Conclusions. The research presented in this study indicates that nursing and midwifery students have insufficient knowledge of DFS treatment methods and biosurgery. Nursing students are more likely than midwifery students to derive knowledge of DFS treatment methods from their studies and the literature. Education on treatment methods and techniques for DFS should be increased among students. Students' knowledge can be increased by classes in the course of their education and meetings with professionals.

Application of <em>Lucilia sericata</em> Larvae in Debridement of Pressure Wounds in Outpatient Settings

Biological therapy using Lucilia sericata larvae has numerous advocates worldwide, yet it is still fairly unknown and not commonly applied in daily practice because of the limited awareness and insufficient experience of medical and nursing personnel. There are case reports suggesting that maggot therapy can be applied and supported by lay caregivers, provided they are supervised and informed by physicians/nurses. The foregoing observation suggests that the method should be considered for implementation by a wider group of caregivers if accepted and meticulously supervised by trained and experienced medical staff. The concerns related to the therapeutic use of maggots in certain regions seem understandable, but are not supported by scientific facts. It should be noted that many therapeutic agents (including brood) used in medicine are of natural origin, and are associated with low production costs and high possibilities of implementation in the course of therapy. By analyzing the literature and using our own clinical and research experience, we have come to conclusions related to using larvae therapy, as a quick and safe method providing cleaning and revitalization in the process of treating wounds of various etiologies, especially pressure ulcers. In the current study, medical-grade Lucilia sericata maggots were applied to remove necrotic tissue from deep pressure sores. The treatment is mostly accepted by both caregivers and patients. In most cases, it is conducted by trained and experienced medical personnel in home and outpatient settings. Over the course of the conducted analyzes involving the collected specimens, there were no statistically significant relationships (p> 0.05) confirmed between the wound surface successfully cleared by brood and variables, such as time from wound formation, location, surface size, and the depth of damage to the tissue structure. The lack of statistical dependence may result from the small size of the studied group. Based on the current findings, we have formulated the following conclusions: Maggot Debridement Therapy (MDT) is a fast and effective method enabling the preparation of the wound bed. The use of MDT in outpatient settings is safe and acceptable for patients and their caregivers.