This paper examines negation and types of tense negation in Urhobo. It also identifies negation marker(s) and the manner in which these negation marker(s) are used in sentences. Transformational generative grammar theory of analysis was used in the work. The aim of this study is to determine the syntactic characteristics of negation in Urhobo. The study shows that negative construction in the Urhobo language involves the doubling of the last vowel of the last word in sentences; or what may be called the lengthening of the last vowel of the lexical item in the sentence. Also, the low-high tone can do the same function as the lexical or grammatical tone. It was observed that negation is a natural phenomenon that cuts across Urhobo, and that the orthographic representation of the low tone, which is the copying of the final vowel, is written contiguously while other negative markers are written separately. It was also observed that Urhobo operates suffixation. Finally, the study work reveals ejo, je, odie and and oyen as negative markers in Urhobo.