Lower Limit Research Articles

Nonlinear properties of uni-directional cellulose nanofiber-reinforced poly (lactic acid) composite filaments: a multiscale computational study

In the quest to elevate the mechanical properties of cellulose nanofiber-reinforced poly (lactic acid) composite materials for robust industrial applications, we conducted multiscale nonlinear finite element analysis rooted in homogenization theory. Our study delved into the intricate web of mechanical mechanisms and their pivotal influencers. This paper zeroed in on three paramount factors: the distribution of fibers, the fiber–matrix interface, and the aspect ratio of the fibers. For fiber distribution, we assumed fiber orientation control in fused filament fabrication and clarified the limit of material improvement by setting one-dimensional orientation as the upper limit. Our analysis of the fiber–matrix interface proposed a comprehensive three-scale structure, encompassing microstructures featuring intricate interfacial phases around the fibers, sub-microstructures with myriad dispersed fibers, and macrostructures subjected to external loads. The profound impact of the fiber–matrix interface on material properties was unveiled through a meticulous two-step homogenization process. Furthermore, we examined the substantial influence of the fiber aspect ratio on material properties. By unraveling the complex interplay among these critical factors, this research provided new design guidelines for fiber morphology to the performance of cellulose composites.

SERS biosensors based on catalytic hairpin self-assembly and hybridization chain reaction cascade signal amplification strategies for ultrasensitive microRNA-21 detection.

A highly sensitive surface-enhanced Raman scattering (SERS) biosensor has been developedfor the detection of microRNA-21 (miR-21) using an isothermal enzyme-free cascade amplification method involving catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR). The CHA reaction is triggered by the target miR-21, which causes hairpin DNA (C1 and C2) to self-assemble into CHA products. After AgNPs@Capture captures the resulting CHA product, the HCR reaction is started, forming long-stranded DNA on the surface of AgNPs. A strong SERS signal is generated due to the presence of a large amount of the Raman reporter methylene blue (MB) in the vicinity of the SERS "hot spot" on the surface of AgNPs. The monitoring of the SERS signal changes of MB allows for the highly sensitive and specific detection of miR-21. In optimal conditions, the biosensor exhibits a satisfactory linear range and a low detection limit for miR-21 of 42.3 fM. Additionally, this SERS biosensor shows outstanding selectivity and reproducibility. The application of this methodology to clinical blood samples allows for the differentiation of cancer patients from healthy controls. As a result, the CHA-HCR amplification strategy used in this SERS biosensor could be a useful tool for miRNA detection and early cancer screening.

Collaborative CRISPR-Cas System-Enabled Detection of Circulating Circular RNA for Reliable Monitoring of Acute Myocardial Infarction.

Acute myocardial infarction (AMI) is one of the major causes of death worldwide, posing significant global health challenges. Circular RNA (circRNA) has recently emerged as a potential diagnostic biomarker for AMI, providing valuable information for timely medical care. In this work, a new electrochemical method for circRNA detection by engineering a collaborative CRISPR-Cas system is developed. This system integrates the unique circRNA-targeting ability with cascade trans-cleavage activities of Cas effectors, using an isothermal primer exchange reaction as the bridge. Using cZNF292, a circulating circRNA biomarker for AMI is identified by this group; as a model, the collaborative CRISPR-Cas system-based method exhibits excellent accuracy and sensitivity with a low detection limit of 2.13 × 10-15 m. Moreover, the method demonstrates a good diagnostic performance for AMI when analyzing whole blood samples. Therefore, the method may provide new insight into the detection of circRNA biomarkers and is expected to have great potential in AMI diagnosis in the future.

The synergistic effect of MnSe2/pyridine diketopyrrolopyrrole-grafted graphene oxide composite for sensitivity electrochemical detection of 4-nitrochlorobenzene

In view of the hazards and degradation inertia of 4-nitrochlorobenzene (4-NCB), it is very important to detect the content of 4-NCB in water samples. Here, we developed a simple platform to accurately monitor the content of 4-NCB based on MnSe2/pyridine diketopyrrolopyrrole-grafted graphene oxide nanocomposite (MnSe2/PDPP-rGO) constructed glass carbon electrode (GCE) sensor. MnSe2/PDPP-rGO composited has been synthesized by two-step hydrothermal method, and MnSe2 nanoclusters composed of nanorods grows uniformly on the surface of PDPP-rGO lamella. Combined with electrochemical activity of MnSe2 to 4-NCB and the excellent conductivity of PDPP-rGO which have been confirmed by cyclic voltammetry and electrochemical impedance spectroscopy, the as-prepared MnSe2/PDPP-rGO/GCE displays outstanding electrochemical sensing comprehensive performance to 4-NCB with wide linear range of 0.05–280 μM, low limit of detection of 4.8 nM, long stability for up to 20 days, satisfactory interference and repeatability. These results provide sufficient basis for accurate and practical detection of 4-NCB based on MnSe2/PDPP-rGO/GCE platform.

Light axion-like particles and the pseudoscalar mesonic decays M⃗M′νν¯

The axion-like particle (ALP) is one kind of the best-motivated new particles. We consider its production from the pseudoscalar mesonic decays M→M′a , with M being a pseudoscalar meson B or K. The upper limits on the flavor-conserving ALP–quark coupling parameter g u are obtained by assuming the ALP to be an invisible particle. We find that the most severe constraint on g u comes from the decay K+→π+νν¯ for 0.05 GeV ≤ M a ≤ 0.35 GeV, while the decays B+,0→K+,0νν¯ and B+,0→π+,0νν¯ can also generate significant constraints.

A ~40-kb flavi-like virus does not encode a known error-correcting mechanism

It is commonly held that there is a fundamental relationship between genome size and error rate, manifest as a notional "error threshold" that sets an upper limit on genome sizes. The genome sizes of RNA viruses, which have intrinsically high mutation rates due to a lack of mechanisms for error correction, must therefore be small to avoid accumulating an excessive number of deleterious mutations that will ultimately lead to population extinction. The proposed exceptions to this evolutionary rule are RNA viruses from the order Nidovirales (such as coronaviruses) that encode error-correcting exonucleases, enabling them to reach genome lengths greater than 40 kb. The recent discovery of large-genome flavi-like viruses (Flaviviridae), which comprise genomes up to 27 kb in length yet seemingly do not encode exonuclease domains, has led to the proposal that a proofreading mechanism is required to facilitate the expansion of nonsegmented RNA virus genomes above 30 kb. Herein, we describe a ~40 kb flavi-like virus identified in a Haliclona sponge metatranscriptome that does not encode a known exonuclease. Structural analysis revealed that this virus may have instead captured cellular domains associated with nucleic acid metabolism that have not been previously found in RNA viruses. Phylogenetic inference placed this virus as a divergent pesti-like lineage, such that we have provisionally termed it "Maximus pesti-like virus." This virus represents an instance of a flavi-like virus achieving a genome size comparable to that of the Nidovirales and demonstrates that RNA viruses have evolved multiple solutions to overcome the error threshold.

Same-day discharge after laparoscopic hysterectomy for benign/premalignant disease: A multicentre randomised controlled trial.

To evaluate whether physical function and quality of life was influenced by discharge on the same-day after a total laparoscopic hysterectomy. Multicentre non-inferiority randomised controlled trial. Five teaching hospitals and two university hospitals in the Netherlands. Patients undergoing laparoscopic hysterectomy for benign or premalignant disease. Following informed consent, participants were allocated 1:1 either to same-day discharge (SDD) or next-day discharge (NDD). The primary outcome was physical function at 7 days after surgery measured by the Patient Reported Outcomes Measurement Information System (PROMIS) Physical Function short Form 10A. Secondary outcomes were physical function and quality of life at 1 and 3 days and 6 weeks after surgery measured with PROMIS short Form 10A and the EuroQol questionnaire (EQ-5D-5L). Two hundred and five patients were included of whom 105 were allocated to SDD and 100 to NDD. Physical function 7 days after surgery was 35.95 in the SDD group and 35.63 in the control group (mean difference 0.32; 95% CI [0.07-0.57]). As the upper limit of the 95% CI does not exceed the non-inferiority margin of 4 points, non-inferiority of SDD could be demonstrated. No difference in physical function nor quality of life on Days 1 and 3 and 6 weeks could be found. This research demonstrates same-day discharge after laparoscopic hysterectomy is non-inferior to next day discharge in physical function 7 days after surgery.

Lead-Free Bismuth-Based Perovskite X-ray Detector with High Sensitivity and Low Detection Limit.

Bismuth-based halide perovskites have shown great potential for direct X-ray detection, attributable to their nontoxicity and advantages in detection sensitivity and spatial resolution. However, the practical application of such materials still faces the critical challenge of combining both high sensitivity and low detection limits. Here, we report a new type of zero-dimensional (0D) perovskite (HIS)BiI5 (1, where HIS2+ = histamine) with high sensitivity and a low detection limit. Structurally, the strong N-H···I hydrogen bonds between HIS2+ cations and inorganic frameworks enhance the rigidity of the structure and diminish the intermolecular distance between adjacent inorganic [Bi2I10]4- dimers. By virtue of such structural merits, single crystal 1 exhibits excellent physical properties perpendicular to both the (001) and (010) faces. Perpendicular to the (010) face, 1 exhibited a high electrical resistivity (2.31 × 1011 Ω cm) and a large carrier mobility-lifetime product (μτ) (2.81 × 10-4 cm2 V-1) under X-ray illumination. Benefiting from these superior physical properties, it demonstrates an excellent X-ray detection capability with a sensitivity of approximately 103 μC Gyair-1 cm-2 and a detection limit of 36 nGyair s-1 in both directions perpendicular to the (001) and (010) crystal faces. These results provide a promising candidate material for the development of new, lead-free, high-performance X-ray detectors.

Search for high-mass exclusive diphoton production with tagged protons in proton-proton collisions at s=13 TeV

A search is presented for high-mass exclusive diphoton production via photon-photon fusion in proton-proton collisions at s=13 TeV in events where both protons survive the interaction. The analysis utilizes data corresponding to an integrated luminosity of 103 fb−1 collected in 2016–2018 with the central CMS detector and the CMS and TOTEM precision proton spectrometer (PPS). Events that have two photons with high transverse momenta (pTγ>100 GeV), back-to-back in azimuth, and with a large diphoton invariant mass (mγγ>350 GeV) are selected. To remove the dominant inclusive diphoton backgrounds, the kinematic properties of the protons detected in PPS are required to match those of the central diphoton system. Only events having opposite-side forward protons detected with a fractional momentum loss between 0.035 and 0.15 (0.18) for the detectors on the negative (positive) side of CMS are considered. One exclusive diphoton candidate is observed for an expected background of 1.1 events. Limits at 95% confidence level are derived for the four-photon anomalous coupling parameters |ζ1|<0.073 TeV−4 and |ζ2|<0.15 TeV−4, using an effective field theory. Additionally, upper limits are placed on the production of axionlike particles with coupling strength to photons f−1 that varies from 0.03 TeV−1 to 1 TeV−1 over the mass range from 500 to 2000 GeV. © 2024 CERN, for the CMS and TOTEMs Collaboration 2024 CERN

Cobalt-Doped Ceria Sensitizer Effects on Metal Oxide Nanofibers: Heightened Surface Reactivity for High-Performing Chemiresistive Sensors.

Chemiresistive gas sensors based on semiconducting metal oxides typically rely on noble metal catalysts to enhance their sensitivity and selectivity. However, noble metal catalysts have several drawbacks for practical utilization, including their high cost, their propensity for spontaneous agglomeration, and poisoning effects with certain types of gases. As such, in the interest of commercializing the chemiresistive gas sensor technology, we propose an alternative design for a noble-metal-free sensing material through the case study of Co-doped ceria (Co-CeO2) catalysts embedded in a SnO2 matrix. In this investigation, we utilized electrospinning and subsequent calcination to prepare Co-CeO2 catalyst nanoparticles integrated with SnO2 nanofibers (NFs) with uniform particle distribution and particle size regulation down to the sub-2 nm regime. The resulting Co-CeO2@SnO2 NFs exhibited superior gas sensing characteristics toward isoprene (C5H8) gas, a significant biomarker for monitoring the onset of various diseases through breath diagnostics. In particular, we identified that the Co-CeO2 catalysts, owing to the transition metal doping, facilitated the spillover of chemisorbed oxygen species to the SnO2 sensing body. This resulting in the sensor having a 27.4-fold higher response toward 5 ppm of C5H8 (compared to pristine SnO2), exceptionally high selectivity, and a low detection limit of 100 ppb. The sensor also exhibited high stability for prolonged response-recovery cycles, attesting to the strong anchoring of Co-CeO2 catalysts in the SnO2 matrix. Based on our findings, the transition metal-doped metal oxide catalysts, such as Co-CeO2, demonstrate strong potential to completely replace noble metal catalysts, thereby advancing the development of the commercially viable chemiresistive gas sensors free from noble metals, capable of detecting target gases at sub-ppm levels.

A novel biosensor based on Y2O3/BN composite for detection of lactate content in athlete training

A novel lactate biosensor based on a yttrium oxide/boron nitride (Y2O3/BN) nanocomposite modified electrode was developed. The BN nanosheets provided a supporting matrix for the Y2O3 nanoparticles, combining their synergistic properties for electrochemical biosensing. The Y2O3/BN nanocomposite provided high surface area and excellent electrocatalytic effects, promoting the oxidation of hydrogen peroxide generated from lactate oxidase catalyzed lactate oxidation. The Y2O3/BN modified biosensor showed wide linear range from 0.005 to 500 μM, low detection limit of 2 nM, high sensitivity of 1.23 μA/μM, good reproducibility with 3.2 % RSD, and long-term stability over 56 days. The biosensor could detect lactate levels in athlete sweat samples with high accuracy and correlation to standard methods, distinguishing changes in sweat lactate corresponding to different exercise intensities. The nanocomposite modified biosensor showed potential for non-invasive, real-time monitoring of exercise levels and fatigue status based on sweat lactate detection in athletic training applications.

A Piezoelectric Nanogenerator-Driven Dual-Mode Platform for Visualization and Impedance Sensing.

Traditional visual biosensing platforms rely on color to display detection results, which can be influenced by individual visual abilities, equipment, parameters, and lighting conditions during photo capture. This limitation significantly impedes the advancement of next-generation portable electrochemical biosensors. Therefore, we propose a visual biosensing device that utilizes distance as an indicator, enabling the facile determination of the length of discoloration, which is inversely proportional to the concentration of the target analyte. The separation of the Signal Generation (SG) and Signal Output (SO) regions effectively mitigates potential interference from the sample color. Additionally, the SG region can be disassembled to facilitate electrochemical impedance spectroscopy (EIS) detection in laboratory settings, enabling dual-mode detection. Meanwhile, the utilization of piezoelectric nanogenerators (PENG) empowers the entire point-of-care testing (POCT) sensing device, effectively addressing the issue of a limited battery life. The biosensing device exhibited a satisfactory linear range (EIS mode, 5 pg/L to 5 mg/L; visual mode, 0.5 ng/L to 5 mg/L) and a low limit of detection (EIS mode, 2.3 pg/L; visual mode, 0.14 ng/L) with S/N = 3 for ochratoxin A (OTA) under optimized conditions. The self-powered and cost-effective dual-mode biosensing platform developed for OTA detection offers clear and easily interpretable results, demonstrating a high accuracy in laboratory settings.

Erythropoietic protoporphyrias: Pathogenesis, diagnosis and management.

The erythropoietic protoporphyrias consist of three ultra-rare genetic disorders of the erythroid heme biosynthesis, including erythropoietic protoporphyria (EPP1), X-linked protoporphyria (XLEPP) and CLPX-protoporphyria (EPP2), which all lead to the accumulation of protoporphyrin IX (PPIX) in erythrocytes. Affected patients usually present from early childhood with episodes of severe phototoxic pain in the skin exposed to visible light. The quantification of PPIX in erythrocytes with a metal-free PPIX ≥3 times the upper limit of normal confirms the diagnosis. Protoporphyria-related complications include liver failure, gallstones, mild anaemia and vitamin D deficiency with reduced bone mineral density. The management is focused on preventing phototoxic reactions and treating the complications. Vitamin D should be supplemented, and DEXA scans in adults should be considered. In EPP1, even in cases of biochemically determined iron deficiency, supplementation of iron may stimulate PPIX production, resulting in an increase in photosensitivity and the risk of cholestatic liver disease. However, for patients with XLEPP, iron supplementation can reduce PPIX levels, phototoxicity and liver damage. Because of its rarity, there is little data on the management of EPP-related liver disease. As a first measure, any hepatotoxins should be eliminated. Depending on the severity of the liver disease, phlebotomies, exchange transfusions and ultimately liver transplantation with subsequent haematopoietic stem cell transplantation (HSCT) are therapeutic options, whereby multidisciplinary management including porphyria experts is mandatory. Afamelanotide, an alpha-melanocyte-stimulating hormone analogue, is currently the only approved specific treatment that increases pain-free sunlight exposure and quality of life.

Constraints on short gamma-ray burst physics and their host galaxies from systematic radio follow-up campaigns

Abstract Short gamma-ray bursts (GRBs) are explosive transients caused by binary mergers of compact objects containing at least one neutron star. Multi-wavelength afterglow observations provide constraints on the physical parameters of the jet, its surrounding medium, and the microphysics of the enhanced magnetic fields and accelerated electrons in the blast wave at the front of the jet. The synchrotron radio emission can be tracked for much longer than in other spectral regimes, and it can pin down the evolution of the spectral peak. We present the results of a systematic observing campaign of eight short GRBs with the MeerKAT radio telescope. Additionally, we present observations of four of these short GRBs using the ATCA radio telescope and two of these short GRBs with the e-MERLIN radio telescope. Using these results we report one possible detection of a short GRB afterglow from GRB 230217A and deep upper limits for the rest of our short GRB observations. We use these observations to place constraints on some of the physical parameters, in particular those related to electron acceleration, the circumburst density, and gamma-ray energy efficiency. We discuss how deeper observations with new and upgraded telescopes should be able to determine if the gamma-ray efficiency differs between long and short GRBs. We also report detections of the likely host galaxies for four of the eight GRBs and upper limits for another GRB, increasing the number of detected host galaxies in the radio with implications for the star formation rate in these galaxies.

Dark photon constraints from a 7.139 GHz cavity haloscope experiment

The dark photon is a promising candidate for the dark matter which comprises most of the matter in our visible Universe. Via kinetic mixing with the Standard Model it can also be resonantly converted to photons in an electromagnetic cavity, offering novel experimental possibilities for the discovery and study of dark matter. We report the results of a pathfinder dark photon dark matter cavity search experiment performed at Hunan Normal University and the Institute of Physics, Chinese Academy of Sciences, representing the first stage of the Axion and dark Photon EXperiment program. Finding no statistically significant excess, we place an upper limit on the kinetic mixing parameter |χ|<3.7×10−13 around mA≃29.5 μeV at 90% confidence level. This result exceeds other constraints on dark photon dark matter in this frequency range by roughly an order of magnitude. Published by the American Physical Society 2024

Lateral Globe Exposure Associated With Graves Upper Eyelid Retraction: The Influence of the Superior Complex Enlargement and Proptosis.

To investigate the association of the lateral exposure of the globe with proptosis and the enlargement of the superior complex (SC) (levator palpebrae superioris muscle + superior rectus muscle) in patients with Graves upper eyelid retraction. Twenty-eight patients with upper lid retraction secondary to Graves orbitopathy and 28 controls matched by age and gender participated in the study. The variables exophthalmometry, margin-reflex distance 1, and upper lid fissure temporal and nasal areas were assessed. Coronal CT scans were evaluated for the measurement of the SC area. A 5% level of significance was assumed for analysis. Abnormal temporal nasal area differences (T-N), defined as values above the superior limit of the control distribution (15.58mm2) were detected in 18 patients. Multiple regression analysis showed a significant correlation of abnormal temporo-nasal asymmetries with the SC area and the proptosis (T-N = -6.7 + 0.62* proptosis + 12.3 SC area, R2 = 0.37; p = 0.003). The etiology of the enhanced lateral globe exposure is not unifactorial. SC enlargement and, to a lesser extent, proptosis are important factors correlated to lateral globe exposure.

Noise analysis of six pulsars and a limit on the gravitational wave background

The direct detection of ultra-low frequency gravitational waves (GWs, 10−9 − 10−6 Hz) using pulsar timing arrays (PTAs) is a significant application of millisecond pulsars. The initial detection of GWs in the nano-Hertz range will probably be an isotropic stochastic GW background (GWB) emitted by the combination of merging super-massive black hole binaries (SMBHBs). In this study, we utilize data from six pulsars collected with four European PTA (EPTA) telescopes to place an upper limit on the GWB amplitude A=2.34−1.32+0.75×10−15 (95%) as a function of the spectral slope α = − 2/3, corresponding to a GWB generated by inspiraling SMBHBs. The posterior for the amplitude that we obtained from the six brightest EPTA pulsars is strongly consistent with previously published upper limits set by the three PTAs. We also compare the sensitivity of our reprocessed pulsar pulse times of arrival (ToAs) determined by the superior ToA creation method, which utilizes a combination of the best template generation algorithm and template-matching algorithm, with the ToAs determined by the EPTA groups. Our analysis shows that the reprocessed ToAs obtained with the superior ToA creation method yield narrower posteriors, indicating an overall increased sensitivity to GW.

All-Fiber Flexible Electrochemical Sensor for Wearable Glucose Monitoring

Wearable sensors, specifically microneedle sensors based on electrochemical methods, have expanded extensively with recent technological advances. Today’s wearable electrochemical sensors present specific challenges: they show significant modulus disparities with skin tissue, implying possible discomfort in vivo, especially over extended wear periods or on sensitive skin areas. The sensors, primarily based on polyethylene terephthalate (PET) or polyimide (PI) substrates, might also cause pressure or unease during insertion due to the skin’s irregular deformation. To address these constraints, we developed an innovative, wearable, all-fiber-structured electrochemical sensor. Our composite sensor incorporates polyurethane (PU) fibers prepared via electrospinning as electrode substrates to achieve excellent adaptability. Electrospun PU nanofiber films with gold layers shaped via thermal evaporation are used as base electrodes with exemplary conductivity and electrochemical catalytic attributes. To achieve glucose monitoring, gold nanofibers functionalized by gold nanoflakes (AuNFs) and glucose oxidase (GOx) serve as the working electrode, while Pt nanofibers and Ag/AgCl nanofibers serve as the counter and reference electrode. The acrylamide-sodium alginate double-network hydrogel synthesized on electrospun PU fibers serves as the adhesive and substance-transferring layer between the electrodes. The all-fiber electrochemical sensor is assembled layer-by-layer to form a robust structure. Given the stretchability of PU nanofibers coupled with a high specific surface area, the manufactured porous microneedle glucose sensor exhibits enhanced stretchability, superior sensitivity at 31.94 μA (lg(mM))−1 cm−2, a broad detection range (1–30 mM), and a significantly low detection limit (1 mM, S/N = 3), as well as satisfactory biocompatibility. Therefore, the novel electrochemical microneedle design is well-suited for wearable or even implantable continuous monitoring applications, thereby showing promising significant potential within the global arena of wearable medical technology.

An Ultrasensitive Biosensor for Probing Subcellular Distribution and Mitochondrial Transport of l-2-Hydroxyglutarate.

l-2-Hydroxyglutarate (l-2-HG) is a functionally compartmentalized metabolite involved in various physiological processes. However, its subcellular distribution and mitochondrial transport remain unclear owing to technical limitations. In the present study, an ultrasensitive l-2-HG biosensor, sfLHGFRH, composed of circularly permuted yellow fluorescent protein and l-2-HG-specific transcriptional regulator, is developed. The ability of sfLHGFRH to be used for analyzing l-2-HG metabolism is first determined in human embryonic kidney cells (HEK293FT) and macrophages. Then, the subcellular distribution of l-2-HG in HEK293FT cells and the lower abundance of mitochondrial l-2-HG are identified by the sfLHGFRH-supported spatiotemporal l-2-HG monitoring. Finally, the role of the l-glutamate transporter SLC1A1 in mitochondrial l-2-HG uptake is elucidated using sfLHGFRH. Based on the design of sfLHGFRH, another highly sensitive biosensor with a low limit of detection, sfLHGFRL, is developed for the point-of-care diagnosis of l-2-HG-related diseases. The accumulation of l-2-HG in the urine of patients with kidney cancer is determined using the sfLHGFRL biosensor.

Adrenalectomy for pheochromocytoma: Surgical outcomes and preoperative risk factors for hemodynamic instability.

Surgical resection for pheochromocytoma (PCC) is still challenging. This study assessed the perioperative outcomes of adrenalectomy for PCC and investigated the risk factors for intraoperative hemodynamic instability (HI). This retrospective study included 571 patients with adrenal tumors who underwent adrenalectomy at Kobe University Hospital and other related hospitals between April 2008 and October 2023. The perioperative outcomes of laparoscopic adrenalectomy were compared between PCC (n = 92) and non-PCC (n = 464) groups. In addition, we investigated several potential risk factors for intraoperative HI in patients with PCC (n = 107; open, n = 11; laparoscopic, n = 92; robot-assisted, n = 4). While patients with PCC had a significantly larger amount of blood loss in comparison to those with non-PCC (mean, 70 and 30 mL, respectively; p = 0.004), no significant difference was observed in the rate of perioperative grade ≥III complications (1.1% vs. 0.6%; p = 0.516), and no perioperative mortality was observed in either group. A tumor size of ≥40 mm, with preoperative hypertension and urinary metanephrines at a level ≥3 times the upper limit of the normal value, were found to be significant predictors of HI, with odds ratios of 2.74 (p = 0.025), 3.91 (p = 0.005), and 3.83 (p = 0.004), respectively. Our data suggest that laparoscopic adrenalectomy for PCC may be as safe as that for other types of adrenal tumors and that large tumors and hormonally active disease may be risk factors for intraoperative HI. The optimal perioperative management for PCC with these risk factors should be established.