ObjectiveTo examine the lower limb kinematics and muscle activities in diagonal single-leg rebound jump in soccer players with chronic ankle instability (CAI). DesignCross-sectional study. SettingLaboratory. ParticipantsThirty male collegiate soccer players participated: 15 with CAI were compared with 15 without CAI, matched by physical description. Intervention(s)In the diagonal single-leg rebound jump, participants stood on one leg on a 30-cm high box, hopped down diagonally (45°) onto a force plate, and jumped vertically as high as possible with hands on their hips. Main outcome measuresHip, knee, and ankle kinematics were acquired using a motion capture system. The activity of the gluteus medius, hip adductor, and lower leg muscles was recorded using electromyography. Jump performance was calculated using a force plate. ResultsThe CAI group had (i) decreased hip adduction, knee flexion, external rotation, and dorsiflexion angle; (ii) reduced hip adductor and peroneus muscle activations; and (iii) reduced jump height and short flight time. ConclusionsMale collegiate soccer players with CAI showed altered kinematics and muscle activities during a diagonal single-leg rebound jump; this may adversely affect rebound jump performance.