AbstractSubantarctic Mode Water (SAMW) is one of the most important water masses for the global ocean uptake and storage of heat and carbon. Based on Argo observations, this study focus on the Southeast Indian Subantarctic mode water (SEISAMW), and investigates the changes of SEISAMW spreading and associated mechanisms. SEISAMW is formed through air‐sea interaction as low potential vorticity water in late winter and subducts into permanent thermocline between outcrop lines of 26.6 σθ and 26.9 σθ in Southern Indian Ocean (SIO). After subduction, the SEISAMW spreads northwestward into subtropical gyre and southeastward into Antarctic Circumpolar Current (ACC) separately. During Argo period, the percentage of SEISAMW volume spreading into SIO subtropics decreases at light layer (26.6–26.7 σθ) while increases at medium (26.7–26.8 σθ) and dense (26.8–26.9 σθ) layer. These changes are attributed to the meridional shifts of outcrop lines. The outcrop lines of 26.6 σθ and 26.7 σθ in the central of SIO (75°–90°E), where light SEISAMW forms, shift poleward. The poleward shifts of outcrop lines result in less light SEISAMW spreading into subtropical gyre. In contrast, outcrop lines of 26.7 σθ and 26.8 σθ south of Australia shift equatorward and favor more medium and dense SEISAMWs spreading into subtropical gyre. The shifts of outcrop lines are closely related to the enhancement of Southern Annular Mode.