Low Germination Percentage Research Articles

Effect of temperature, pretreatments, gibberellin (GA3), salt and drought stress on germination of Thymus satureioides coss of Morocco

Thymus satureioides Cosson is an endemic medicinal and aromatic plant species of both Morocco and Algeria, holds significant cultural and economic importance. It is used in traditional medicine to treat various diseases and also as a culinary spice herb. T. satureioides has become a key export for Morocco. However, increasing market demand and the effects of climate change have led to a decline in its wild populations, raising concerns about the scarcity of this valuable natural resource. To address these challenges, a comprehensive understanding of the environmental requirements for the germination of T. satureioides is essential for planning and prioritizing conservation efforts in its native habitats. In this context, the aim of this study was to assess the combined effects of various pretreatments and temperature and of salinity, drought stress, and gibberellin on the germination parameters of T. satureioides. Seeds were collected from a wild plant population in the Anti-Atlas Mountains. The tests included five pretreatments (mechanical scarification, sulfuric acid (95%), dry heat, boiling water and hydrogen peroxide) in conjunction with five temperature conditions (10, 15, 20, 25 and 30 °C). Additionally, six concentrations of sodium chloride (0, 25, 50, 100, 150 and a 200 mM), six water potential levels of polyethylene glycol (PEG-6000) (0, −0.07, −0.14, −0.22, −0.32 and - 0.53 MPa) and six concentrations of gibberellin (0, 50, 100, 500, 1000 and 2000 ppm) were tested. Results showed that seeds can germinate without any treatment. However, seeds treated with dry heat followed by incubation at 20 °C improved all the germination parameters. In addition, germination parameters progressively decreased and delayed with increasing levels of both salt and water stress. Without stress, the germination percentage was 100%, with increasing salt stress to 200 mM germination was completely inhibited. Although, seeds showed a low germination percentage (5,67%) at the highest osmotic potential (−0.53 MPa).

Effect of Meteorological Parameter on Growth and Yield of Different Varieties of Field Pea (Pisum sativum L.) in the Bundi District, Rajasthan, India

A field experiment was conducted in a farmer field of Bundi district, Rajasthan, Department of Environmental Science and Natural Resources Management College of Forestry, Sam Higginbottom University of Agriculture, Technology and Sciences Prayagraj, during Rabi 2020-2021. The experiment used a factorial randomized block design with three different planting dates: D115 October, D2–5 November, and D3– 25 November, as well as three different varieties: V1- Annapurna, V2-Azad -111, and V3- SS- 10. The findings discovered The maximum germination percentage (78.51%) and lowest germination percentage (66.78%) of seeds per plot were found in plots D1 and V1 (15th October + Annapurna). The maximum number of branches (4.00) were found in D1 and V3 (15th October + SS-10), while the lowest number of branches were found in D3 and V1 (25th November + Annapurna) (2.33). D1 and V1 (15th November + Annapurna) reported the highest plant height of 26.00, 91.38, and 124.15cm at 30, 60, and 90 DAS, whereas D3 and V3 (25th November + SS-10) recorded the minimum plant height of 19.00, 25.54, and 67.95cm at 30, 60, and 90 DAS. D3 and V3 (05th November + SS-10) had the highest blooming percentage (50.41), whereas D3 and V2 (25th November + Aazad-111) had the lowest flowering percentage (46.18). D1 and V3 (15th October + SS-10) had the highest seeds/pods (7.0), whereas D3 had the fewest (4.0).D2 and V3 (05th November + SS-10) had the highest grain yield (12.68), whereas D3 and V1 (25th November + Annapurna) had the lowest grain yield (11.01). Variety-1 plant height was shown to have a substantial positive relationship (Annapurna).

Seed Dormancy Characteristics of Kadsura coccinea (Lem.) A. C. Smith, a Unique Medicinal Plant in Southeast Asia

Kadsura coccinea (Lem.) A. C. Smith is a significant tree species of non-timber forest. However, the low germination percentage and lengthy germination time of its seeds pose obstacles to industry development. Aiming at the problem of seed dormancy, this study first determined the seed dormancy period through seed germination test, and then comprehensively evaluated the dormancy characteristics by observing the seed coat structure, measuring endogenous inhibitors, and in vitro embryo culture. The results indicated the dormancy of K. coccinea seeds, and that the germination period was up to 80 days. Its seed coat is composed of exotesta and endotesta. The dense seed coat structure causes water absorption and air permeability obstacles to the seeds and causes mechanical restraint to the development of the embryo. Meanwhile, its seeds have substances that inhibit seed germination, and there are germination inhibitors in distilled water, methanol, petroleum ether, and ethyl acetate extracts of kernels and seed coats. The inhibitory activity of kernel petroleum ether extract was the highest, and the inhibitory activity of seed coat methanol extract was the highest. In addition, the embryo of K. coccinea developed completely and could germinate normally under in vitro conditions. This study has basically proved that the dormancy of K. coccinea seeds is caused by the seed coat (physical dormancy) and endogenous inhibitors (physiological dormancy), which provides a scientific theoretical basis to further explore the method of seed dormancy release of K. coccinea.

Studies on Seed Borne Mycoflora of Soybean Seeds by Incubation Methods

The present study was conducted to detect and identify seed-borne pathogenic fungi in some soybean varieties and their effect on seed germination. Six varieties of soybean seed samples viz.JS 95-60, JS 97-52, RSC 10-46, RSC 10-71, CG Soya-1 and local variety were selected for the experiment. Twelve seed-borne mycoflora viz. Aspergillus flavus, Aspergillus niger, Alternaria spp., Cladosporium spp., Curvularia spp., Rhizopus spp., Trichoderma spp., Rhizoctonia spp., Nigrospora spp., Macrophomina spp., Fusarium spp. and Penicillium spp. were detected and identified from the seeds of six soybean varieties by using the standard blotter method, agar plate method, 2,4-D blotter method, roll paper towel method and deep freeze method. The seed sample of local variety recorded the highest percentage of mycoflora with the lowest germination percentage, whereas CG Soya-1 variety found as the least percentage of mycoflora with the highest germination percentage compared to other varieties of soybean taken in the study. Among all of the incubation methods, the agar plate was found as the most effective in detecting seed-related mycoflora, on soybean seeds.

Germination and sustainable cultivation of succulent halophytes using resources from a degraded estuarine area through soil technologies approaches and saline irrigation water

AbstractThe degradation of estuarine areas has been a growing concern in recent years, as these ecosystems offer several services. The Arthrocnemum and Suaeda genera produce useful compounds with various applications, which have led to their overexploitation in their native habitats. The main goal of this study is to use green technologies for sustainable cultivation of two species bioindicators of Mediterranean saltmarshes, Arthrocnemum macrostachyum and Suaeda vera, as an alternative to their overutilization. A tailored soil (TAIL) was constructed with a Saline Fluvisol (FLU), from a marginal estuarine area, and organic/inorganic residues were used as amendments, for plant cultivation. Seeds collected in natural populations were pretreated with two dormancy‐breaking treatments (H2SO4, H2O2) and germinated in FLU or TAIL. A microcosm assay using FLU or TAIL irrigated with estuarine water (VF) or three aqueous saline solutions (0, 200, or 400 mmol/L NaCl) was established. The species obtained a low percentage of seeds' germination being the highest values under substrate control (water‐soaked filter paper). The A. macrostachyum reached the highest germination percentage when seeds were pretreated with H2SO4 (20%) while the germination of S. vera was favored by the pretreatment with H2O2 (49%). Both species had the highest growth in TAIL irrigated with 0 or VF, presenting the largest stems (13–32 cm), the largest primary branches (3.5–7.2 cm), and the highest production of aerial part biomass (28–36 g). This study contributes to the sustainable cultivation of these valuable halophytes and could play an important role in the sustainable management of marginal estuarine land.

SMAX1 interacts with DELLA protein to inhibit seed germination under weak light conditions via gibberellin biosynthesis in Arabidopsis

Karrikins (KARs) were first identified as a class of small-molecule chemicals derived from smoke that promote seed germination. However, the implied mechanism is still not well understood. Here, we find that KAR signaling mutants have a lower germination percentage than that of wild type under weak light conditions, and KARs promote seed germination through transcriptional activation of gibberellin (GA) biosynthesis via SMAX1. SMAX1 interacts with the DELLA proteins REPRESSOR of ga1-3-LIKE 1 (RGL1) and RGL3. The interaction enhances the transcriptional activity of SMAX1 and inhibits GIBBERELLIN 3-oxidase 2 (GA3ox2) gene expression. The KAR signaling mutant seed germination defect under weak light is partially rescued by exogenous application of GA3 or by GA3ox2 overexpression, and the rgl1 rgl3 smax1 triple mutant exhibits higher germination rates under weak light than the smax1 mutant. Thus, we show a crosstalk between KAR and GA signaling pathways via a SMAX1-DELLA module in regulating seed germination in Arabidopsis.

Influence of “El Niño Southern Oscillation” periods on plant density of Hoffmannseggia aphylla (Fabaceae) an endangered species

Backgrounds and aims: Studies on the natural regeneration of plant species under hyperaridity conditions have been scarce, mainly because of the low germination percentage of the species under these conditions. Presumably, sporadic phenomena such as the El Niño Southern Oscillation (ENSO) could have a positive effect on the natural germination of these species, although this effect has not yet been satisfactorily explored. M&M: To test the crucial assumption of our statement, a hyperarid region (average rainfall below 5 mm/year) was used as a model to determine the effect of ENSO years on the natural regeneration rate of adult individuals of the endemic legume Hoffmannseggia aphylla (retama) in the Atacama Desert, northern Chile. Thus, the vegetation density of an endemic leguminous species in a sector of the Pampa del Tamarugal, Tarapacá Region, northern Chile, was analyzed for six years. Results: The density of this species increased during ENSO years, mainly due to water flows from the highest sectors of the Tamarugal pampas, such as the town of Pica, in addition to the increase in humidity and summer rainfall. Conclusion: Our results highlight the transcendence of the ENSO in the regeneration of leguminous plants.

The role of micropyle for grass pea germination

PurposeThis study aims to identify the location of the micropyle, the role of the micropyle in seed germination and the association between the micropyle size and seed weight of grass peas.Design/methodology/approachFirst, the micropyle was identified by cutting the seed in half and observing the seeds under the electron microscope. Second, the micropyle was covered by lanolin to block water imbibition. The rate of imbibition and germination was then observed. Lastly, micropyle sizes of various grass pea genotypes were identified by capturing seed images under a light microscope and converting the sizes to mm2 using computer software (ImageJ).FindingsThe location of micropyle was located nearby the hilum, similar to soybean seeds. Seed imbibition was significantly lower in lanolin application (<87%) than in the control (>124%) after 24 hours of submergence. Germination was a day delay for lanolin application on the micropyle compared to lanolin application on the non-micropyle. The germination delay resulted in a significantly lower germination percentage at <57% on the micropyle lanolin application than at >79% on the non-micropyle lanolin application after 10 days of sowing. There is no correlation between the micropyle size and seed weight.Originality/valueThese findings add information on the location and the role of the micropyle for grass pea seed germination.

Improving the Quality of Lunar Regolith Simulant Soil for Future Food Security

This research explores the possibilities of using lunar soil resources in agriculture. The effectiveness of humic micro-encapsulated was investigated for physical and chemical quality improvement for lunar culture applications by using TLS-01 (Thailand artificial lunar regolith Simulant-01) as a test substance. Sunflower (Helianthus annuus L.) was used as the test plant. TLS-01 particles were evenly distributed, uncluttered, and consisted of 54.55% polygons and 45.45% rods, without having high water-holding capacity. The plants were grown in TLS-01, but their physical characteristics were unacceptable. Sunflower seedlings grown in TLS-01 soil showed a lower percentage of germination, and root and stem length compared to those grown in commercial planting material. After an improvement in physical properties and nutrients, from the study of the relationship between the soil used in the trial for planting and the humic acid concentration, no interaction between soil and humic acid was found. Therefore, while the soil types were significantly different, the humic was not. The results showed that a 1:1 mixture of TLS-01: coconut coir with the addition of 2 times the recommended dose of humic acid could enhance sunflower seedling relative to commercial planting material (p≤0.05). The germination percentage and growth indexes were 85.00% and 6.62, respectively. However, in order to stimulate actual usage for interplanetary application, coconut fiber-like materials and the humic substances were applied as in microcapsules. Additionally, the humic release test showed that after 6 h, the release of humic substance by Fickian diffusion was 73.45% and it then slowed down. The encapsulation efficiency was 90.37%.

Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds

BackgroundVanilla planifolia is the most widely cultivated species of vanilla with high economic importance. However, seed germination under artificial conditions is difficult and yields low germination percentages. The seeds are adapted to endozoochorous dispersal, and we therefore tried to simulate the conditions in the digestive tract by acid scarification of seeds.ResultsImmature seeds lacking dormancy, used as a control, showed the highest germination percentage. Among the treatments tested for mature seeds, the hydrochloric acid treatments were significantly the best in breaking dormancy and inducing germination, irrespective of the acid concentration and the presence of pepsin. Conventional treatment with a hypochlorite solution induced much lower germination percentage. Sulphuric acid at concentration 50% was too strong and caused damage to the seeds. Important factor is also high cultivation temperature 30 °C as there was nearly no germination at 25 °C.ConclusionsOur protocol significantly improves the efficiency of generative propagation of vanilla and allows for significantly higher germination percentages than previously described. The strongly positive effect of hydrochloric acid may be related to the adaptation of seeds to endozoochorous dispersal.

A test of local adaptation to drought in germination and seedling traits in populations of two alpine forbs across a 2000 mm/year precipitation gradient.

Seed regeneration is a critical stage in the life histories of plants, affecting species' abilities to maintain local populations, evolve, and disperse to new sites. In this study, we test for local adaptations to drought in germination and seedling growth of two alpine forbs with contrasting habitat preferences: the alpine generalist Veronica alpina and the snowbed specialist Sibbaldia procumbens. We sampled seeds of each species from four populations spanning a precipitation gradient from 1200 to 3400 mm/year in western Norway. In a growth chamber experiment, we germinated seeds from each population at 10 different water potentials under controlled light and temperature conditions. Drought led to lower germination percentage in both species, and additionally, slower germination, and more investment in roots for V.alpina. These responses varied along the precipitation gradient. Seeds from the driest populations had higher germination percentage, shorter time to germination, and higher investments in the roots under drought conditions than the seeds from the wettest populations - suggesting local adaption to drought. The snowbed specialist, S.procumbens, had lower germination percentages under drought, but otherwise did not respond to drought in ways that indicate physiological or morphological adaptions to drought. S.procumbens germination also did not vary systematically with precipitation of the source site, but heavier-seeded populations germinated to higher rates and tolerated drought better. Our study is the first to test drought effects on seed regeneration in alpine plants populations from high-precipitation regions. We found evidence that germination and seedling traits may show adaptation to drought even in populations from wet habitats. Our results also indicate that alpine generalists might be more adapted to drought and show more local adaptations in drought responses than snowbed specialists.

Morphological and anatomical characterization of colchicine-induced polyploids in watermelon

This study aimed to elucidate the effective colchicine concentration to induce polyploidization distinction based on morphological and anatomical features in watermelon. Watermelon seeds were soaked in different colchicine concentrations (0.05, 0.1 and 0.5%) for several durations (24, 48 and 72 h) to induce polyploidization. Putative polyploids were evaluated regarding their morphological and anatomical traits compared to diploids (control). A colchicine concentration of 0.5% for 72 h resulted in the lowest germination percentage with high mortality in putative polyploids compared to in diploids. Morphological traits revealed vigorous growth in putative tetraploids with slower germination speeds, whereas the putative octoploids had suppressed growth compared to tetraploids and diploids. Regarding reproductive biology, the petal number (6), pollen size and viability were remarkably higher in induced polyploids, which confirmed successful tetraploid induction by 0.5% colchicine treatment for 72 h. Similarly, a bigger stomatal size with a lower density was also noticed in induced tetraploids compared to in diploids using the same treatment after anatomical analyses. Meanwhile, a PCA and correlation matrix illustrated that, among the 20 variables, polyploid induction efficiency (PIE), leaf length (LL), guard cell distance (GCD), and pollen viability (PV) were recognized as the most effective morphological and anatomical indicators for successful polyploid induction confirmation with colchicine in watermelon. The present findings provide a basis for distinguishing colchicine-induced polyploids as improved genetic resources to enhance seedless triploid breeding in watermelon.

SEED PRIMING AND ITS FUTURE PROSPECT: A REVIEW

Production and productivity of crops is highly affected by uneven germination and poor crop establishment. The greater the germination percentage of the seed, the greater will be the productivity. Problems like low seed replacement rate, climate change, drought, low germination percentage, poor crop stand etc. are influencing the crop production. There might be the introduction of scientific tools and techniques in crop production like improved variety, resistance variety, etc. But each and every farmer is not capable and skilled enough to utilize advanced technology. In that case, seed priming can be a better alternative as well as a better solution to the problems. It is cost effective, ecofriendly and easily accessible to every farmer out there. It helps in better germination of seed, increases root vigor, improves crop establishment there by increasing yield. So, Seed priming has the capacity to close the gap between potential yield an actual yield.

Gibberellic acid in the emergence and early development of star fruit seedlings

Star fruit (Averrhoa carambola L.) is an exotic fruit originating in Southeast Asia and has seeds with low germination. The objective was to evaluate the effect of gibberellin on the emergence and initial development of star fruit seedlings. The seeds were removed from the fruits and washed in running water, and placed in gibberellin solution 1,000 mg.L-1, 2,000 mg.L-1, 3,000 mg.L-1, 4,000 mg.L-1 and pure water as a control. for 30 minutes. Sowing was carried out by placing 1 seeds per 280 mL tube in a substrate containing ravine soil + tanned manure (3:1) in a randomized block experimental design. Thirty days after the emergence of the first seedling, the percentage of emergence, emergence speed index and mean emergence time were evaluated. Ninety days after the emergence of the first seedling, the seedling height, collar diameter, number of leaves, root length were evaluated; green and dry mass of the leaves, green and dry mass of the roots. The star fruit seed has a low percentage of germination, with low emergence rates, and the use of gibberellin was efficient to increase and accelerate emergence, showing a positive response to the variables plant height, number of leaves, stem diameter and length of the seed. source. The treatment with gibberellic acid had a positive effect on the emergence and development of star fruit seedlings, and on the mass production of leaves and roots, with the concentration of 1,000 mg.L-1 showing the best results.

Effect of Leaf Extracts of Lantana Camara L. on Germination and Growth of Some Crops Species

Allelopathy is a biotic interaction between plants where one plant inhibits the germination and growth of other plants by releasing certain allelochemicals. In this research we evaluated the allelopathic effect of aqueous leaf extract of Lantana camara on the germination and growth of four crop plants. The experiment was conducted in sterilized petri dishes in Botany lab of Saptagandaki Multiple Campus, Bharatpur, Chitwan, Nepal. The effect of different concentrations of extracts was studied and compared with the control. The results revealed that the germination and seedling growth of all species were significantly inhibited at different concentrations as compared to the control. The lowest germination percentage and seedling growth was found in Brassica campestris and Oryza sativa plant respectively and effect was least in Triticum aestivum plant at 10% concentration. The germination percentages, seedling growth and, biomass in all species were observed to reduce, with the increasing concentration of aqueous extracts. The study concluded that Lantana camara leaf extract allelochemicals have adverse effects on germination, seedling growth and dry matter accumulation of all tested crops.

Allelopathic Effects of Some Fruit Plant Species with Weeds

Aqueous extracts of seven fruit plant species viz., banana (Musa paradisiaca), Indian gooseberry (Phyllanthus emblica), jack fruit (Artocarpus heterophyllus), guava (Psidium guajava), mango (Mangifera indica), litchi (Litchi chinensis), wood apple ((Aegle marmelos) were tested for germination, radical and plumule growth of barnyard grass and green amaranth. The lowest germination percentage (9.5 and 14.4%), plumule (3.94 and 0.92 cm) and radicle length (0.825 and 0.495 cm) of barnyard grass and green amaranth seedlings were obtained in seed treated with wood apple aqueous extract due to presence of some toxic compounds or other inhibitory materials in the species. One of these plant extract which performed the best i.e. wood apple leaves extract has the potential for use as an alternative crop as a herbicide (protectants) against barnyard grass and green amaranth weed species.

BIOCIDAL-EFFICACY OF GORGONEIA LATIFOLIUM, MYRISTICA FRAGANS AND ALPRON- PLUS ON SEED HEALTH, GERMINATION PERCENTAGE, SEEDLING VIGOUR OF SWEET MELON (CUCUMIS MELO L.) SEEDS AND ASSOCIATED FUNGI ORGANISMS

Laboratory experiment was conducted to test the effects of bio-medicinal plants (Gongronema latifolium (Utazi) leaves and Myristica fragans (Nutmeg) seeds) and synthetic fungicide (Alpron- Plus) on seed health, germination percentage and seedling vigour of sweet melon seeds. The experiment was conducted at the Crop Science and Horticulture Laboratory, Nnamdi Azikiwe University Awka, Anambra State. Bio-medicinal plants were used as crude powdery seed dressers as well as synthetic fungicide. The experiment was laid out in a simple completely randomized design (CRD) with ten replications which consisted of blotter paper germination/ seed health tests of sweet melon seeds and seedling vigour and seedling vigour index tests using soil medium. Ten petri dishes and ten polybags were used respectively for each of the plant extracts and synthetic fungicides simultaneously for both blotter paper and soil medium tests. Twenty seeds which were independently dressed with various plant extracts and synthetic fungicide were plated into one 9cm Petri dish containing three layers of blotter paper in circular form. For soil medium method, ten polybags with few small perforations underneath filled with sterilized top soil were used for each fungicide. The Petri dishes containing the plated seeds were incubated on the Laboratory benches for eight days while seedlings in polybags were kept in the screenhouse and observations were taken and recorded daily for germination count, fungal growth then germination index and seedling vigour index which were derived later with appropriate formulae and their values being expressed in percentages. The results of this investigation revealed that bio-medicinal plant crude extracts had significant effect on germination percentage and germination index of Sweet- melon seeds. Gongronema latifolium crude extract had the highest germination index. The synthetic fungicide was effective in protecting the seeds of sweet melon though had low germination percentage. Nutmeg was not effective when used in crude powdery form as dressers as it had the lowest germination percentage and the highest infestation percentage. It could therefore be recommended that the plant extracts tested in this experiment should not be used as powdery seed dressers but as essential oil using appropriate extracting solvents in order for the bioactive ingredients to act effectively in preventing micro-organisms that hinder germination of sweet melon seeds.

Germinação de sementes e substratos para mudas de Melocactus zehntneri

ABSTRACT The populations of Melocactus zehntneri 'chapéu-de-frade' have shown an alarming decline due to the constant withdrawal of individuals from their natural areas. The low percentage of germination of M. zehntneri seeds, added to the slow growth, requires a system for commercial propagation of this species. This study aimed to test the influence of water, hydrochloric acid or acetic acid pre-treatments on seed germination and the influence of different substrates on the initial development of M. zehntneri seedlings. The seeds were immersed in deionized water, hydrochloric acid (5N) or glacial acetic acid (100%) for 10, 20, 30, 40 and 50 minutes and placed to germinate in a Petri dish containing filter paper moistened with 5 mL deionized water. The seedlings obtained from this germination (≡ 0.4 cm diameter) were cultivated on Carolina Soil® and vermiculite [2:1] substrates; Carolina Soil® and vermiculite [2:1] + drainage; medium-grained sand; medium-grained sand + drainage; medium-grained sand and organic compost [1:1]; medium-grained sand and organic compost [1:1] + drainage; and Carolina Soil® and medium-grained sand [1:1]. The highest percentage of germination was obtained with the pre-treatment by immersion in water, in the times of 10 and 30 minutes, totaling 55% of germinated seeds. Treatments with hydrochloric acid did not increase the percentage of germinated seeds, which were also close to 50%. In the test with the substrates, in Carolina Soil® there were the best rates of seedling survival after transplanting (87.5%) and seedling development performance. Treatments containing organic compost resulted in lower survival (69%) and seedling development. Seed germination and seedling development in Carolina Soil® substrate and sand proved to be an excellent alternative for the production of M. zehntneri seedlings.

Influence of Seed Priming on Germination of Pea (Pisum sativum)

Purpose: The Seed priming is an efficient method for synchronizing germination and increasing seed vigor. The experiment was performed in Sindh Agriculture University, Tando Jam, Sindh, Pakistan, to study and quantify the effect of different types of seed priming on the seed germination percentage and shoot and root length of pea.
 Methodology: The experiment was laid down in randomized complete block design with split plot arrangements. Five different priming treatments we used; T1 Soaking in water for 12 hours, T2 Soaking in 50oC hot water for 10 minutes, T3 Soaking in 2% NaCl solution for 10 minutes, T4 Soaking in 50% H2SO4 solution for 10 minutes, T5 Control (no priming).
 Findings: The results indicated a significant/ high effect of the priming treatments on the germination percentage, length of shoot, and length of roots. The highest germination percentage about (98%), the highest length of shoot about (4.26 cm), and the highest length of root about (10.92 cm) were observed from T3. The lowest germination percentage about (76%), the lowest length of shoot about (2.24cm), or the lowest length of root about (1.12 cm), result was observed from the (T5). These treatments were followed by germination percentage of about (94%), shoot length about (3.54 cm), and the length of root about (9.74 cm) from T2. It was followed by germination percentage of (88%) shoot length about (3.34 cm), length of root about (8.28 cm) from T1. This was followed by the germination percentage about (86%), shoot length about (2.44 cm), and length of root about (8.4 cm) from T4.
 Conclusion: Result of this experiment show significant effect of the priming treatment on the majority of seed germination and growth of components in the pea grain, including seed germination percentage, shoot and root length of pea.
 Recommendations: The study recommended that farmers to adopt seed priming. These practices among smallholder’s farmers should focus on enhancement of farmer awareness and know about seed priming. When farmers see that seed does not germinate properly like they expect, they should apply seed priming treatments.

Extent of Adoption of Agricultural Practices in Sugarcane Production in Response to Climate Change in Coastal Odisha

The present research was conducted to assess the extent of adoption of different agricultural practices in sugarcane production in response to climate change. The research study was conducted in Jagatsinghpur district of Odisha . 2 blocks, 4 Gram panchayats and 8 villages were selected for the study 60 numbers of respondents by purposive sampling method. Ex-post facto research design was chosen in this research. The findings of the study showed that giving protective irrigation in response to late monsoon with a mean score 1.60 was the most adopted practice where resowing in case of low germination percentage with a mean score 1.17 was the least adopted practice in the research area.