Low Load Density Research Articles

A systematic approach for estimating colloidal particle adsorption model parameters

The estimation of ion-exchange chromatography model parameters is crucial to enable efficient model-assisted biopharmaceutical downstream process development. Model calibration methods can be hindered by model limitations combined with parameter correlations, leading to time-consuming repeated parameter estimations. While Steric Mass Action isotherm estimation methods exist, there is a need for a systematic approach to estimate model parameters for an emerging Colloidal Particle Adsorption (CPA) model proposed by Briskot et al. This study presents a novel strategy that addresses this challenge, offering significant improvements.Through a parameter sensitivity analysis, we identified key levers for improved CPA parameter estimation, enabling the prediction of elution behavior for low and high load densities in gradient and step elution mode. This analysis also revealed the correlation structure of parameters, allowing the establishment of a minimalized experimental data set for parameter estimation, by using one breakthrough, a high load and three low load density gradient elution experiments. Our workflow leverages a surrogate-assisted global-optimization tool, minimizing computationally expensive function evaluations during parameter fitting. Furthermore, we employed a customized objective function, specifically adapted to the model structure and sensitivity results, to enhance the solver's performance.Our strategy was tested on three model proteins with molecular weights of approximately 50, 150 and 200 kDa using a strong cation exchange Poros 50 HS resin. Our final approach enabled high throughput CPA model calibration for single components. The resulting CPA models were able to describe non-binding protein-pulses, low and high-loaded gradient elution, break through, as well as isocratic elution experiments.

Plasmon-Enhanced Photoelectrochemistry of Photosystem II on a Hierarchical Tin Oxide Electrode for Ultrasensitive Detection of 17β-Estradiol.

Despite its excellent efficiency in natural photosynthesis, the utilization of photosystem II (PSII)-based artificial photoelectrochemical (PEC) systems for analytical purposes is hindered due to the low enzyme loading density and ineffective electron transfer (ET) processes. Here, we present a straightforward and effective approach to prepare a PSII-based biohybrid photoanode with remarkable photoresponse, enabled by the use of a hierarchically structured inverse-opal tin oxide (IO-SnO2) electrode combined with gold nanoparticles (Au NPs). The porous, carbon-containing IO-SnO2 structure allows for a high density and photoactivity loading of PSII complexes, while also providing strong electrical coupling between the protein film and the electrode. A new electron transfer pathway mediated by Au NPs was identified at the protein-electrode interface, which efficiently shuttles the photogenerated electrons from the enzyme to the IO-SnO2 electrode. Furthermore, the PEC response of the electrode was significantly enhanced by the surface plasmon resonance (SPR) effect of Au NPs. Upon light irradiation, this PSII-based photoanode exhibited an impressively high and stable photocurrent output, which was utilized to fabricate an aptasensor for 17β-Estradiol (E2) detection. Under optimal conditions, a detection limit of 0.33 pM was obtained, along with a broad detection range from 15 pM to 30 nM. The applicability of the aptasensor was assessed by measuring E2 in water and urine samples, demonstrating its feasibility in environmental monitoring and clinical tests.

Influence of Loading Density and Gender on the Welfare and Meat Quality of Horses During Transport for Slaughter.

The aim of this study was to determine the effects of loading density and gender on blood welfare indicators, carcass bruises and horsemeat quality. Data were collected from twelve transports of 89 slaughter horses originating from the same collection centre. The transportation of slaughter horses at high loading densities (>200 kg/m2), especially stallions, resulted in increased levels of lactate (p = 0.021), glucose (p < 0.0001), ceruloplasmin (p < 0.0001) and AOPP (p < 0.0001), but lower GSH levels (p < 0.0001). Compared to stallions and mares, geldings subjected to high loading density (>200 kg/m2) during transport had lower levels of the aforementioned blood metabolites. In addition, stallions exposed to a high load density (>200 kg/m2) had the highest frequency of severe (p = 0.0002), large (p < 0.0001) and circular (p = 0.0001) carcass bruises, which were predominantly located on the abdominal (p = 0.0056) and thoracic (p = 0.0004) wall. In contrast, a higher percentage (p < 0.0001) of undamaged carcasses was found in slaughter horses exposed to a low loading density (≤200 kg/m2) during transport. Stallions exposed to high loading densities (>200 kg/m2) during transport had the highest initial pH (p < 0.0001) and ultimate pH (p = 0.005) in terms of m. longissimus lumborum and redness (p = 0.017), but the lowest drip loss (p = 0.050) and lightness (p < 0.0001), which, consequently, led to the highest (p = 0.0045) proportion of DFD-like defects in the meat quality. In conclusion, the results of this study showed that high loading densities (>200 kg/m2), regardless of gender, negatively affect horse welfare during transport. In addition, stallions were more sensitive to poor pre-slaughter conditions and produced the lowest meat quality, while geldings were the most resistant. To determine the optimal transport density, further research is needed to determine the effects of different loading densities on the behaviour, physiology, carcass and meat quality of slaughter horses.

Constructing LiF‐Enriched Solid Electrolyte Interface on Graphene Arrays with Abundant Edges on Microscale Si‐C Anodes Toward High‐Energy Lithium‐Ion Batteries

AbstractSilicon (Si) anodes offer excellent lithium storage capacity for lithium‐ion batteries but face practical limitations due to significant volume expansion and low intrinsic electrical conductivity. These issues lead to side reactions that consume the electrolyte and impede ion‐electron transport, resulting in low areal loading (&lt;2 mg cm⁻²) and restricted energy density. To address this, a scalable method is developed using spray drying of commercial graphite flakes (s‐Gr) and nanosilicon particles (n‐Si), followed by chemical vapor deposition to create microscale Si/C anodes (s‐Gr/n‐Si/VGs). Thin vertical graphene nanosheets (VGs) are grown on the surfaces and within the internal pores, forming a robust, micron‐sized Si/C spherical composite material. The VGs construct the conductive network, allowing the electrodes to operate at high areal loadings without pulverization and promoting LiF‐enriched solid electrolyte interphase for improved cycling stability. The s‐Gr/n‐Si/VGs maintain a capacity of 641.9 mAh g⁻¹ after 1000 cycles at 11.0 mg cm⁻², retaining 95.9% capacity. In pouch cells with NCM811 cathodes, the 5.0 Ah‐level cells achieved 80.0% capacity retention after 510 cycles at 1.0 C. This research provides a feasible pathway for manufacturing high‐performance, low‐cost, and scalable Si/C anodes suitable for high‐energy‐density lithium‐ion batteries.

Direct Anchoring of Molybdenum Sulfide Molecular Catalysts on Antimony Selenide Photocathodes for Solar Hydrogen Production.

Molybdenum sulfide serves as an effective nonprecious metal catalyst for hydrogen evolution, primarily active at edge sites with unsaturated molybdenum sites or terminal disulfides. To improve the activity at a low loading density, two molybdenum sulfide clusters, [Mo3S4]4+ and [Mo3S13]2-, were investigated. The Mo3S x molecular catalysts were heterogenized on Sb2Se3 with a simple soaking treatment, resulting in a thin catalyst layer of only a few nanometers that gave up to 20 mA cm-2 under one sun illumination. Both [Mo3S4]4+ and [Mo3S13]2- exhibit catalytic activities on Sb2Se3, with [Mo3S13]2- emerging as the superior catalyst, demonstrating enhanced photovoltage and an average faradaic efficiency of 100% for hydrogen evolution. This superiority is attributed to the effective loading and higher catalytic activity of [Mo3S13]2- on the Sb2Se3 surface, validated by X-ray photoelectron and Raman spectroscopy.

Investigating the performance of CoFe2O4@TiO2 nanomaterial as multifunctional cathode catalyst for simultaneous dye degradation and oxygen reduction in microbial desalination cell

Microbial desalination cell (MDC) is an emerging technology in which water is desalinated and energy is generated by the breakdown of organic compounds and catalyzed by microorganisms using the potential gradient created in the reactor. MDC efficacy is substantially influenced by the oxygen reduction reaction (ORR) occurring at the cathode, albeit potential hindrances include sluggish reaction rates and biofouling development. The present study involved synthesizing and utilizing CoFe2O4@TiO2 nanocomposite as a multifunctional catalyst on a cathode surface for efficient ORR and dye removal. XPS, HRTEM, and XRD techniques were used to ascertain the NPs' morphology, surface properties, dimensions, and XRD patterns. Physicochemical investigation showed that the nanoparticles had a consistent distribution, a mean diameter of 18.11 nm, and a spherical shape. Researchers tested MDCs with nanoparticles (NPs). The highest power density was 4.56 W/m3, 50 % more than the MDC with a lower loading density. The desalination efficiency was found to be 60 % at 1.5 mg/cm2 CoFe2O4@TiO2, however, it was 80 and 81 % at 2.5 and 2.0 mg/cm2 catalyst concentrations, respectively. The dye degradation efficiency of as high as 88 % was observed with CoFe2O4@TiO2 nanocomposite cathode MDC. The findings show that CoFe2O4@TiO2 nanocomposite might be an economically feasible ORR catalyst, improving MDC efficiency and cost.

Locally Ordered Single-Atom Catalysts for Electrocatalysis.

Single-atom catalysts manifest nearly 100 % atom utilization efficiency, well-defined active sites, and high selectivity. However, their practical applications are hindered by a low atom loading density, uncontrollable location, and ambiguous interaction with the support, thereby posing challenges to maximizing their electrocatalytic performance. To address these limitations, the ability to arrange randomly dispersed single atoms into locally ordered single-atom catalysts (LO-SACs) substantially influences the electronic effect between reactive sites and the support, the synergistic interaction among neighboring single atoms, the bonding energy of intermediates with reactive sites and the complexity of the mechanism. As such, it dramatically promotes reaction kinetics, reduces the energy barrier of the reaction, improves the performance of the catalyst and simplifies the reaction mechanism. In this review, firstly, we introduce a variety of compelling characteristics of LO-SACs as electrocatalysts. Subsequently, the synthetic strategies, characterization methods and applications of LO-SACs in electrocatalysis are discussed. Finally, the future opportunities and challenges are elaborated to encourage further exploration in this rapidly evolving field.

Locally Ordered Single‐Atom Catalysts for Electrocatalysis

AbstractSingle‐atom catalysts manifest nearly 100 % atom utilization efficiency, well‐defined active sites, and high selectivity. However, their practical applications are hindered by a low atom loading density, uncontrollable location, and ambiguous interaction with the support, thereby posing challenges to maximizing their electrocatalytic performance. To address these limitations, the ability to arrange randomly dispersed single atoms into locally ordered single‐atom catalysts (LO‐SACs) substantially influences the electronic effect between reactive sites and the support, the synergistic interaction among neighboring single atoms, the bonding energy of intermediates with reactive sites and the complexity of the mechanism. As such, it dramatically promotes reaction kinetics, reduces the energy barrier of the reaction, improves the performance of the catalyst and simplifies the reaction mechanism. In this review, firstly, we introduce a variety of compelling characteristics of LO‐SACs as electrocatalysts. Subsequently, the synthetic strategies, characterization methods and applications of LO‐SACs in electrocatalysis are discussed. Finally, the future opportunities and challenges are elaborated to encourage further exploration in this rapidly evolving field.

Mathematical Modelling of Resonant Electric Power Transmission System

The most significant feature of the power supply to agricultural consumers is the uneven distribution of the electrical load across three phases of the 0.4 kilovolt four-wire power grid and low load density (515 kilowatts per square kilometer) and coverage of large areas. Based on modern requirements for the quality of electricity and reliability of power supply to agricultural consumers, there is a need to modernize existing and create a new generation of rural electric grid. One of the variants of such grid is a resonant power transmission system. (Research purpose) The research object is a physical and mathematical model of a resonant system, taking into account a wide selection of the necessary physical parameters for the transfer function, the research purpose is determining the parameters of the resonant system. (Materials and methods) Used Mathematica version 4.2.0.0. It was established that all physical parameters can be set in accordance with the experiment. (Results and discussion) A new type of electrical systems for electric power transmission with the use of a resonant method of electric power transmission was considered. A mathematical model of a high-frequency line based on a single-wire line with a return through the ground was given. (Conclusions) We investigated the general principle of operation, the main components, and also calculated electrical characteristics, including power, frequency response, efficiency, features of operation, including wireless communication. The calculation results were compared with the existing equipment with a transmission power of up to 8000 watts. It was shown that on the basis of the obtained equations, it is possible to correctly select the parameters of the resonant power transmission line and voltage converters. It was determined that the obtained physical and mathematical model of a resonant single-conductor system, taking into account a wide selection of the necessary physical parameters, makes it possible to understand the operation of a resonant power transmission line and build optimized receiving and transmitting equipment complexes.

Ru-core Ir-shell electrocatalysts deposited on a surface-modified Ti-based porous transport layer for polymer electrolyte membrane water electrolysis

Novel Ru-core Ir-shell catalyst-integrated porous transport electrodes (PTEs) for polymer electrolyte membrane water electrolysis (PEMWE) cells are prepared, in which Ru-core Ir-shell catalyst nanostructures are directly deposited onto a porous transport layer (PTL) via arc plasma deposition (APD). The PTL has a nanostructured TiO2 surface prepared via NaOH etching, acting as a catalyst support. The performance and durability of these Ru-core Ir-shell catalysts depend strongly on the ratio of Ir and Ru. The current-voltage (I–V) characteristics of PEMWE cells were improved by applying these core-shell catalysts with a low Ir loading of around 0.1 mg cm−2. The core-shell catalyst-integrated PTEs can operate at current densities of up to 10 A cm−2 without exhibiting limiting current behavior. This unique combination of the core-shell catalyst and the PTE structure enables PEMWE cell operation with low iridium loading and high current density, potentially reducing the cost of green hydrogen.

Magnetic field induced the assembling of Photothermal evaporator for efficient solar‐driven desalination

AbstractSolar‐driven interfacial evaporation is a sustainable and economical technology for freshwater generation. Structural design of photothermal material is an effective strategy to improve the evaporation performance but is usually bothered by complicated processes and non‐adjustability. Herein, magnetic nanoparticles assembled photothermal evaporator was developed, which showed an adjustable spinal array surface under uniform magnetic field induction. By regulating position in the magnetic field, the desirable surface structures could be uniform at relatively low load density of magnetic nanoparticles to improve light absorption via multiple reflections. Magnetic field induced evaporator could accelerate evaporation to over 1.39 kg m−2 h−1 under 1‐sun illumination, which was 2.8 times that of natural evaporation. After coated by carbon layer, magnetic nanoparticles could overcome the oxidation to realize stable evaporation in long‐term desalination. The facile strategy to optimize the surface structure via magnetic field is appropriate for various fields with special requirements on surface structure.image

Antibody aggregation can lead to reduced Protein A binding capacity and low step yield

Protein A affinity chromatography has been widely used for antibody capture and initial purification. In general, the yield of this step is relatively high (i.e., >90%). However, recently while purifying a bispecific antibody (bsAb) in appended IgG format, the Protein A capture step experienced low yield (i.e., ∼80%). It was found that the target bsAb started appearing in flow-through at a relatively low load density, suggesting that a portion of the expressed bsAb has compromised Protein A binding capability. Further studies indicated that the bsAb in flow-through was mainly in aggregate form. In addition, normal Protein A step yield was restored when the column was loaded with bsAb monomer. Thus, all the evidence pointed to the fact that aggregate with compromised binding capacity was the cause of low Protein A step yield in this case.

Dense T-Nb2O5/Carbon Microspheres for Ultrafast-(Dis)charge and High-Loading Lithium-Ion Batteries.

Orthorhombic niobium pentoxide (T-Nb2O5) is regarded as a potential anode material for lithium-ion batteries (LIBs) due to ultrafast charge/discharge and high safety. However, the poor electronic conductivity and low mass loading of nanostructured T-Nb2O5 limit its practical application in LIBs. Herein, we design and construct dense microspheres consisting of nanostructured T-Nb2O5 embedded in amorphous N-doped carbon (Nb2O5@NC) via a facile method to achieve fast ionic and electronic transport as well as a high mass loading. The dense micro-sized particles with an interconnected carbon network avoid the low mass loading and volumetric energy density of conventional nanostructures. Interconnected pores in the range of a few nanometers are also formed in the Nb2O5@NC microspheres. Notably, at a high mass loading of 12.8 mg cm-2, Nb2O5@NC can achieve a high specific capacity of 171.5 mAh g-1 and an areal capacity of 2.05 mAh cm-2, showing its high lithium storage capacity. The intercalation reaction mechanism with a small volume change during cycling at both crystal lattice and microsphere levels is confirmed by in situ X-ray diffraction and in situ high-resolution transmission electron microscopy. The elegant structure and the electrochemical reaction mechanism disclosed in the work is important for designing ultrafast-(dis)charge electrode materials.

Boosted microwave absorption properties of CoFe2O4 with extraordinary 3D morphologies

Due to their strong magnetic dissipation and low cost, ferrites were one of the first generations of microwave absorbers. However, ferrites also have some drawbacks, such as a low natural resonance frequency (fr), a lack of dielectric loss, and high density. In order to overcome these drawbacks and improve the microwave dissipation features of ferrites, we successfully prepared CoFe2O4 samples with flower-like and crochet ball-like morphologies (named as M1 and M2 samples, respectively). Structural and optical properties were studied by XRD, FTIR, and UV–Vis light absorption. The microwave performance of CoFe2O4 was significantly improved with the reflection loss (RL) of M2 of −40 dB. Furthermore, M1 and M2 samples achieved an ultra-wide effective absorption bandwidth (EAB) of 13 and 12.5 GHz, respectively. It is worth noticing that the EAB of M1 was one of the largest EABs for CoFe2O4 that has been reported so far. The excellent microwave dissipation of M1 and M2 samples in the 2–18 GHz frequency range was due to the enhancement of ferrite fr to the high-frequency range and the introduction of dielectric loss to achieve impedance matching. The flower-like and crochet ball-like morphologies with many pores of M1 and M2 also resolved the high-density issue of CoFe2O4. With the relatively good values of RL and EAB combined with low filler loading, thin thickness, and low density, M1 and M2 samples could be expected to be promising microwave absorbers for practical applications.

Scaling-up electrohydrodynamic drying for energy-efficient food drying via physics-based simulations

Electrohydrodynamic (EHD) drying is a novel non-thermal drying method to dry heat-sensitive foods faster and with lower energy. Upscaling EHD drying to dry large amounts of food is the current challenge of this technology. In this regard, we quantify how successful a newly-proposed electrode configuration for EHD dryers is for drying commercial amounts of fruit for a wide range of operating conditions. To achieve this goal, we simulate an EHD dryer using physics-based modeling. The scalability was evaluated by quantifying the impact of fruit loading density in the dryer, applied voltage, and distance between electrodes on the drying time and energy consumption. Drying fruits in a commercial EHD dryer is more optimal when the dryer is densely loaded, compared to a low loading density. Loading the trays in the dryer to a capacity of 70% increased the drying time by 16%, compared to drying a few fruits widely spaced apart, but the energy consumption was 28% less. We identified the best strategy to dry a particular batch of fruit with EHD drying to achieve the fastest drying with the least energy possible. We found that it is most energy-efficient and quick to load the dryer close to its full capacity, instead of drying smaller batches in many different runs. By loading the trays in the dryer to 70% of their capacity, we could dry 7 times faster and with 11 times less energy in a single drying run instead of drying the same amount of fruit by many different runs. This study presents a key step towards upscaling EHD drying systems for the industry to dry large amounts of fruits in the shortest possible time and more energy-efficiently.

Combined effects of weather conditions, transportation time and loading density on carcass damages and meat quality of market-weight pigs.

This study investigated the interactive effects of weather conditions, transportation time and loading density on carcass damages and meat quality traits of market-weight pigs under commercial conditions. The following pork quality parameters were measured: pH and temperature; colour; drip, thawing and cooking loss; and textural traits. Pigs were assigned to one of eight groups arranged in a 2 2 2 factorial design according to the weather conditions (hot and cold), transportation time (short and long) and loading density (high and low). A three-way ANOVA and Tukey's post-test (multiple comparisons) were performed to assess the differences between groups in examined pork quality traits. Pigs exposed to short transportation ( 20 ) at high loading density (0.29 ) during hot weather conditions produced meat with the lowest initial and ultimate meat pH value and sensory colour scores, and the highest initial temperature and the occurrence of pale, soft and exudative pork. The occurrence of pale, soft and exudative pork was reduced 5-fold during hot weather conditions when pigs were exposed to longer transportation ( 210 ) and low loading density (0.53 ). Pigs exposed to short transportation ( 20 ) at high loading density (0.41 ) during cold weather conditions produced the highest quality pork (the highest percentage of red, firm and non-exudative pork) characterised by lowest drip loss and value and the highest sensory colour scores. The highest percentages of carcass damages were recorded in pigs exposed to both low (0.50 ) and high (0.33 ) loading density during long transportation ( 210 ) in cold weather conditions. In conclusion, weather conditions and loading density are of greater importance for the occurrence of carcass damages and pork quality variation than transportation time.

Loofah sponge as an environment-friendly biocarrier for intimately coupled photocatalysis and biodegradation (ICPB)

Intimately coupled photocatalysis and biodegradation (ICPB) provides novel insights into the removal of refractory pollutants. However, the practical application of ICPB was limited by low bacterial loading density and low microbial activity. Herein, an environment-friendly, low-cost and renewable biocarrier was fabricated using natural loofah sponge. In comparison to polyurethane carrier, after employing loofah sponge in ICPB, the tetracycline hydrochloride (TCH) removal rate was increased by ∼6.6 %, the mineralization was increased by ∼9.2 %, and almost all photocatalytic products were degraded. Compared with conventional catalyst coating strategies, our powder spraying method enables the exterior surface of biocarrier to be firmly coated by the photocatalyst, while the inside of biocarrier was extremely clean to accumulate bacteria. The biocarrier provided a higher loading density and a higher microbial activity of bacteria than that of polyurethane carrier. Besides, it can regulate the bacterial community to have more favorable members to biodegrade the photocatalysis intermediates. In summary, using loofah sponge as biocarrier could be an effective approach for enhancing the biodegradation performance of ICPB.

Investigation on initial atmospheric corrosion of copper and inhibition performance of 2-phenyl imidazoline based on electrical resistance sensors

The initial atmospheric corrosion of copper was investigated by electrical resistance sensor under different environmental factors (relative humidity, temperature, and NaCl loading density). The corrosion rate of Cu increases evidently with NaCl loading density, temperature and RH, but decreases with prolonged test. 2-phenyl imidazoline (2-PI) presents increasing inhibition efficiency on Cu with elevated temperatures at low NaCl loading density (≤40 μg/cm2), mainly due to that elevated temperature enhances the vapour pressure of 2-PI, however, 2-PI shows inferior performance on pre-corroded Cu at high NaCl loading density, possibly due to that the defective corrosion product layer formed at high RH retards the adsorption of 2-PI. Finally, molecular dynamic simulations were conducted to evidence the competitive adsorption advantage of 2-PI against H2O on Cu2O surface through chelation of 1-N of imidazoline with Cu(I).

Влияние потерь электроэнергии на надежность электрооборудования в сельской электросети 0,4 кВ

Rural networks are characterized by a large length and low load density. In the country, there are problems with the reliability of electricity supply to agricultural enterprises and the uninterrupted operation of electrical equipment. The average duration of consumer outages is up to 100 hours annually. In Western developed countries, it is statistically defined as an acceptable state of electricity supply, when for a medium- voltage network during the year, the total duration of interruptions is in the range of 15-60 minutes per year. The permanent functioning of the Russian agro-industrial complex is impossible without reliable and stable operation of rural electric distribution networks. (Research purpose) The research purpose is in studying the impact of electricity losses on the reliability of electrical equipment in the rural power grid of 0.4 kilovolt. (Materials and methods) The methods of ensuring reliability include: design features of the system, reliability of electrical equipment; provision of backup power, system management tools, organization of operation. The reasons for the decrease in reliability were: equipment failures, errors of operating personnel, excess of resource capabilities, deviation of the actual operating conditions of the equipment from the calculated ones. (Results and discussion) The article considers the issues of additional electricity losses in the low- voltage network of 0.4 kilovolts on the example of the power supply of an agricultural enterprise, caused by the flow of reactive energy. The article presents graphs of phase voltages and power consumption. Authors conducted experimental studies in a network of 0.4 kilovolts. The article presents the calculated reliability indicators of electrical equipment at an agricultural enterprise. The increase in reliability when installing a compensating device in the 0.4 kilovolt network are described. (Conclusions) The increase of reliability of electrical equipment is revealed, the probability of failure operation of the electric motors decreased by 3 percent; the probability of failure operation of the electric motors decreased by 19 percent; reducing the failure rate by 15 percent; decrease in failure rates by 14 percent.

High Durability and Electrocatalytic Activity Toward Hydrogen Evolution Reaction with Ultralow Rhodium Loading on Titania

Herein, we report the synthesis of titania supported Rh(0) nanoparticles (Rh0/TiO2) as electrocatalyst for hydrogen evolution reaction (HER) in acidic medium. Rhodium nanoparticles with an average particle size of 2.54 nm are found to be well-dispersed on TiO2 surface. Rh0/TiO2 with very low loading density (3.79 μg cm−2) was attached on the glassy carbon electrode (GCE) by drop-casting method. Electrocatalytic performance of modified GCE was investigated via linear sweep voltammetry (LSV) in 0.5 M aqueous H2SO4 solution after 2000 cycle treatment (Rh0/TiO2-2000) and it was found that Rh0/TiO2-2000 on GCE exhibits superior electrocatalytic activity (TOF: 11.45 s−1 at η = 100 mV, η0:−28 mV, η10 mA cm−2: −37 mV, j0: 0.686 mA cm−2 and Tafel slope: 32 Mv dec−1). More importantly, it provides outstanding long-term stability (10000 cycles) at room temperature for HER, which makes Rh0/TiO2-2000 a promising electrocatalyst for hydrogen generation.