Low Temperature Gasoline Combustion is a novel combustion strategy that employs the use of a fuel like gasoline whose autoignition characteristics are sensitive to varying concentrations of fuel. It has the potential to dramatically reduce nitrogen oxide (NOx) and soot emissions relative to conventional combustion modes with similar thermal efficiency and has the added production benefit of using a widely available fuel. To address efficiency at low loads, a single direct injection (S-DI) is used that stratifies the fuel enough to get sufficient combustion performance. But with the increased fuel stratification, there is the potential to create undesirable fuel distributions that could increase harmful NOx emissions. This work uses three-dimensional computational fluid dynamics (3D-CFD) to investigate the effects of injection parameters including injection pressure, start of injection timing, and intake valve closing temperature on this S-DI strategy using a medium-duty engine at two low load conditions. A computational model was developed and validated to experimental data collected on this engine using Large Eddy Simulations turbulence modeling to resolve the complex flow phenomena and turbulent mixing prevalent in this combustion strategy.Injection pressure was increased in six increments from 120 bar to 342 bar at constant start of injection (SOI) of 45 crank angle degrees before top dead center and the effects on performance and emissions are analyzed at two load operating conditions of 2 and 3 bar. Then, at each injection pressure, the start of injection timing was delayed to analyze its impact on combustion performance. The tradeoffs for performance and emissions with these injection parameters are explored along with suggestions for further investigations to improve this S-DI injection process.