Introduction: This study includes synthesis, characterizations, antimicrobial, antiox-idant, and docking molecular study of novel Bis-Azetidinone compounds that combined two units of β-lactam rings .In the present investigation, the aromatic aldehydes with primary amine were condensed to create Schiff's base, which was then reacted with chloroacetylchloride to pro-duce bis-Azetidinone compounds. Methods: Melting points, FTIR, and NMR spectrum analyses were used to examine the mor-phological and topological characteristics of the Bis-Azetidinone compounds. The results indi-cate that the prepared Compounds synthesis has excellent antimicrobial activity against both Gram-negative (Escherichia coli,), Gram-positive bacteria (Staphylococcus aureus) and fungal (Candida albicans) and also indicated that the Compounds synthesis (A2) gave a higher antimi-crobial effect than the B2, C2. The synergistic activity was examined against the pathogenic microbial strains. It was observed that employing compound synthesis combined with antibiot-ics enhanced the synergistic efficacy compared to using compound synthesis alone or antibiotic alone on Gram-positive bacteria and fungi. Results: The antioxidant efficiency was assessed by DPPH, the results show that the compound synthesis has antioxidant activity, and also indicated that the synthesized compound (A2) gave a higher antioxidant effect than the B2, C2. Docking study confirmed via redocking of crystal-ized substrate or inhibitor within target binding pocket. The docking results reveal that the syn-thesized compounds, with a total binding affinity of less than -48 kcal/mol, could be clinically used for future therapeutic purposes. Conclusion: The present research demonstrates the advantageous effectiveness of a simpler pro-duction procedure, novel Bis-Azetidinone compounds, for producing high-purity with low haz-ard that may be utilized as future possible medical therapies.
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