In order to explore how the ubiquitous stochastic low-frequency (SLF) variability of O-type stars is related to various stellar characteristics, we compiled a sample of 150 O-type stars observed via ground-based spectroscopic surveys, alongside photometric data obtained from the Transiting Exoplanet Survey Satellite (TESS). We analyzed 298 light curves obtained from TESS Sectors 1–65 for the stars in our sample. Leveraging the spectroscopic parameters, we used Bonnsai to determine masses, radii, fractional main-sequence ages, and mass-loss rates for stars of our sample. Subsequently, we identified possible correlations between the fitted parameters of SLF variability and stellar properties. Our analysis unveiled four significant correlations between the amplitude and stellar parameters, including mass, radius, fractional main-sequence ages, and mass-loss rate. For stars with ≳30 M ⊙, we observed a decrease in characteristic frequency and steepness with increasing radius. Finally, we compared various physical processes that may account for the SLF variability with our results. The observed SLF variability may arise from the combined effects of the iron convection zone (FeCZ) and internal gravity waves (IGWs), with IGWs potentially more dominant in the early stages of stellar evolution, and the contribution of FeCZ becoming more significant as stars evolve. Meanwhile, our results indicate that the SLF variability of O-type stars bears certain signatures of the line-driven wind instability and granulation.