Low Detection Limit Research Articles

A Lanthanide-Incorporated Phospho(III)tungstate Aggregate Constructed from [HPIIIW8O31]10- and [W11O39]12- Building Blocks and Its Nanocomposite with CdS for Ultrasensitive Photoelectrochemical Detection of Oxytetracycline.

A novel tartronic acid decorated hexa-CeIII-incorporated phospho(III)tungstate aggregate (C4H12NO)6Na18H2[(HPW8O31)2[W11O39]2(H2TAD)4(H2O)4W4Ce6H2P2O14]·84H2O (1, H3TAD = tartronic acid) was synthesized by a one-step assembly strategy. Its main skeleton is constructed from two [W11O39]12- fragments, two [HPIIIW8O31]10- segments and one H2TAD--ornamented dodecanuclear heterometallic [W4Ce6H2PIII2O14(H2TAD)4(H2O)4]18+ cluster. In the structure, the [HPIIIO3]2- groups not only work as the heteroatom template to induce the formation of lacunary [HPIIIW8O31]10- segments but also function as the connector to bridge Ce3+ cations. With the help of a reaction strategy of combining ultrasonication treatment with the continuous ion layer adsorption method, the 1/CdS composite was constructed and exhibits prominent photoelectrochemical activity. The 1/CdS composite was used as a photoelectrochemical sensor for oxytetracycline detection at 0 V (vs Ag/AgCl), which displays excellent properties with quick response and low limit of detection (0.042 nM). This work can provide some helpful references in the construction of novel PIII-induced polyoxometalates consisting of different building blocks and can extend the applications of polyoxometalate-based nanocomposites into photoelectrochemical detection for antibiotics as well as biomolecules.

HKUST‐1 Derived Cu2S‐Cu3P@C Nanomatrix Electrode Material for Non‐Enzymatic Electrochemical Glucose Sensing

AbstractRobust Cu2S‐Cu3P@C Nanomatrix electrode material has been synthesized via a two‐step process involving carbonization of Cu‐MOF, HKUST‐1, followed by simultaneous phosphorization and sulphurization of the carbonized product. This seamless approach leads to the efficient incorporation of highly active Cu2S‐Cu3P hybrid nanostructures within a conductive carbon matrix, exhibiting exceptional electrochemical glucose sensing performance. Synergistic interactions between Cu2S‐Cu3P and the carbon matrix boost glucose electro‐oxidation kinetics. The resulting Cu2S‐Cu3P@C displays a broad linear response (0.005–5 mM), low detection limit (2.3 μM), and high sensitivity (10,790 μA mM−1 cm−2), with excellent selectivity towards glucose even in the presence of interferents. This biomimetic Cu2S‐Cu3P@C nanomatrix catalyst holds promise for biosensor development in bioanalysis and clinical diagnosis.

Diagnosis with Metagenomic Next-Generation Sequencing (mNGS) technology and real-time PCR for SARS-CoV-2 Omicron detection using various nasopharyngeal swabs in SARS-CoV-2 Omicron.

The SARS-CoV-2 Omicron variant, with the main subtypes BA.5.2 and BF.7 in China, led to off-target effects on the S and N genes from December 1, 2022, to January 31, 2023. The kits used for studying and developing these agents were not adequately and independently evaluated. It is important to verify the performance of commercial Real-Time quantitative PCR (RT-qPCR) tests. We conducted a clinical evaluation of two Real Time SARS-CoV-2 Omicron assays to verify their performance using various detection reagents and clinical specimens. We performed clinical evaluations of two existing Chinese SARS-CoV-2 Omicron RT-qPCR kits 2019-nCoV nucleic acid diagnostic kits (Fosun Biotechnology, National instrument registration 20203400299, Shanghai, China) and COVID-19 nucleic acid detection kits (eDiagnosis Biomedicine, National instrument registration 20203400212, Wuhan, China) and using BSD (Bondson) (Guangzhou Bondson Biotechnology Co. Ltd, batch number 2022101), quality controls provided by the inspection center and a large number of clinically confirmed specimens. The concordance rates for the Fosun and eDiagnosis kits were 95% and 100%, respectively. The detection limit for the Fosun and eDiagnosis kits was verified to be 300 copies/mL and 500 copies/mL. The Fosun assay exhibited the largest coefficient of variation (CV) for ORF1ab and N gene at the detection limit concentration (4.80%, 3.49%), whereas eDiagnosis showed a smaller CV (0.93%, 1.10%). In the reference product from the Hangzhou Clinical Laboratory Center test, it was found that Fosun had the lowest sensitivity of 93.47% and a specificity of 100%, while eDiagnosis exhibited 100% for both sensitivity and specificity. The lowest single target gene detection rate of Fosun reagents was 68.7% for the ORF1ab gene and 87.5% for the N gene, while eDiagnosis detection rate was 100%. Among the clinical group S specimens, the missed detection rate of the Fosun reagent was 10.9%, which was higher than the 3.9% of eDiagnosis. However, there was no significant difference in the clinical diagnostic efficiency of the two reagents. The ORF1ab and N assays of SARS-CoV-2 Omicron on the eDiagnosis platform yielded higher values compared to those on the Fosun platform. Consequently, the eDiagnosis kit has also been used as standard detection reagents. Considering that the Fosun reagent has a relatively low detection limit and targets three single genes, it is more advantageous as a confirmatory reagent for the new museum.

Rapid nanomolar detection of cocaine in biofluids by electrochemical aptamer-based sensor with low-temperature effect for drugged driving screening.

Cocaine is one of the most abused illicit drugs, and its abuse damages the central nervous system and can even lead directly to death. Therefore, the development of simple, rapid and highly sensitive detection methods is crucial for the prevention and control of drug abuse, traffic accidents and crime. In this work, an electrochemical aptamer-based(EAB) sensor based on the low-temperature enhancement effect was developed for the direct determination of cocaine in bio-samples. The signal gain of the sensor at 10°C was greatly improved compared to room temperature, owing to the improved affinity between the aptamer and the target. Additionally, the electroactive area of the gold electrode used to fabricate the EAB sensor was increased 20 times by a simple electrochemical roughening method. The porous electrode possesses more efficient electron transfer and better antifouling properties after roughening. These improvements enabled the sensor to achieve rapid detection of cocaine in complex bio-samples. The low detection limits (LOD) of cocaine in undiluted urine, 50% serum and 50% saliva were 70nM, 30nM and 10nM, respectively, which are below the concentration threshold in drugged driving screening. The aptasensor was simple to construct and reusable, which offers potential fordrugged driving screening in the real world.

Employing conductive porous hydrogen-bonded organic framework for ultrasensitive detection of peanut allergen Ara h1

Peanut allergy has garnered worldwide attention due to its high incidence rate and severe symptoms, stimulating the demand for the ultrasensitive detection method of peanut allergen. Herein, we successfully developed a novel electrochemical aptasensor for ultrasensitive detection Ara h1, a major allergenic protein present in peanuts. A conductive nickel atoms Anchored Hydrogen-Bonded Organic Frameworks (PFC-73-Ni) were utilized as excellent electrocatalysts toward hydroquinone (HQ) oxidation to generate a readable current signal. The developed electrochemical aptasensor offers wide linear range (1–120 nM) and low detection limit (0.26 nM) for Ara h1. This method demonstrated a recovery rate ranging from 95.00% to 107.42% in standard addition detection of non-peanut food samples. Additionally, the developed electrochemical method was validated with actual samples and demonstrated good consistency with the results obtained from a commercial ELISA kit. This indicates that the established Ara h1 detection method is a promising tool for peanut allergy prevention.

WSe2 Negative Capacitance Field-Effect Transistor for Biosensing Applications.

Field-effect transistor (FET) biosensors based on two-dimensional (2D) materials are highly sought after for their high sensitivity, label-free detection, fast response, and ease of on-chip integration. However, the subthreshold swing (SS) of FETs is constrained by the Boltzmann limit and cannot fall below 60 mV/dec, hindering sensor sensitivity enhancement. Additionally, the gate-leakage current of 2D material biosensors in liquid environments significantly increases, adversely affecting the detection accuracy and stability. Based on the principle of negative capacitance, this paper presents for the first time a two-dimensional material WSe2 negative capacitance field-effect transistor (NCFET) with a minimum subthreshold swing of 56 mV/dec in aqueous solution. The NCFET shows a significantly improved biosensor function. The pH detection sensitivity of the NCFET biosensor reaches 994 pH-1, nearly an order of magnitude higher than that of the traditional two-dimensional WSe2 FET biosensor. The Al2O3/HfZrO (HZO) bilayer dielectric in the NCFET not only contributes to negative capacitance characteristics in solution but also significantly reduces the leakage in solution. Utilizing an enzyme catalysis method, the WSe2 NCFET biosensor demonstrates a specific detection of glucose molecules, achieving a high sensitivity of 4800 A/A in a 5 mM glucose solution and a low detection limit (10-9 M). Further experiments also exhibit the ability of the biosensor to detect glucose in sweat.

A minimally invasive sensing system based on hydrogel microneedle patches and Au/Cu2O nanospheres modified screen-printed carbon electrode for glucose monitoring in interstitial skin fluid

Glucose monitoring in interstitial skin fluid (ISF) has become a novel approach for diabetes management. In this paper, a minimally invasive sensing system consisted of methacrylated hyaluronic acid (MeHA) based microneedle patches and Au/Cu2O nanospheres modified screen-printed carbon electrode (SPCE) was designed for glucose monitoring in the ISF of living mice. The highly swellable and biocompatible MeHA microneedle patches can effectively extract ISF from the skin, barely cause pain to the living mice. The glucose level in the extracted ISF was detected via an enzyme-free electrochemical sensor using the Au/Cu2O nanospheres as the electrocatalyst. The doping of Au into Cu2O resulted in a highly conductive and active electrocatalyst for glucose oxidation, which improved the sensing performance of glucose sensor. A wide linear range (0.02 – 6 mM), low detection limit (0.018 mM), and high sensitivity (110.38 μA mM−1 cm−2) were obtained under the optimized condition. The obtained results exhibited a comparable trend to those obtained from commercial glucometers. We have combined successfully MeHA patches for ISF extraction with enzyme-free electrochemical glucose sensor. Therefore, this novel sensing system holds great potential for diabetes management in a minimally invasive manner.

In Situ Defect Engineering of Fe-MIL for Self-Enhanced Peroxidase-Like Activity.

Defect engineering is an effective strategy to enhance the enzyme-like activity of nanozymes. However, previous efforts have primarily focused on introducing defects via de novo synthesis and post-synthetic treatment, overlooking the dynamic evolution of defects during the catalytic process involving highly reactive oxygen species. Herein, a defect-engineered metal-organic framework (MOF) nanozyme with mixed linkers is reported. Over twofold peroxidase (POD)-like activity enhancement compared with unmodified nanozyme highlights the critical role of in situ defect formation in enhancing the catalytic performance of nanozyme. Experimental results reveal that highly active hydroxyl radical (•OH) generated in the catalytic process etches the 2,5-dihydroxyterephthalic acid ligands, contributing to electronic structure modulation of metal sites and enlarged pore sizes in the framework. The self-enhanced POD-like activity induced by in situ defect engineering promotes the generation of •OH, holding promise in colorimetric sensing for detecting dichlorvos. Utilizing smartphone photography for RGB value extraction, the resultant sensing platform achieves the detection for dichlorvos ranging from 5 to 300ngmL-1 with a low detection limit of 2.06ngmL-1. This pioneering work in creating in situ defects in MOFs to improve catalytic activity offers a novel perspective on traditional defect engineering.

Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle.

Detecting glucose accurately and sensitively from clinical samples like tears and saliva is still difficult. We have created a sensor that can detect glucose with high sensitivity and accuracy by combining the use of glucose oxidase (GOx) to catalyze glucose, a pistol-like DNAzyme (PLDz) to transform the signal, gold nanoparticles (AuNPs) to enhance the optical properties and the exonuclease-III (Exo-III) to amplify the signal. As a result, the proposed method exhibits a low detection limit of 7.5pM and a wide detection range covering seven orders of magnitude. The suggested dual-mode strategy provides a sensitive, precise and specific detection method for glucose. Another advantage is that the dual-mode technique significantly improves the precision and consistency of the measurements, demonstrating its immense potential for use in biomedical research and clinical diagnostics.

Transparent Perovskite Wafers via Nanocrystals Ordered Coalescence Toward Sensitive and Stable X‐Ray Detection and Imaging

AbstractMetal halide perovskite wafers have shown significant potential in large‐area X‐ray detection and imaging. However, a distinct difference in optical transparency between state‐of‐the‐art perovskite wafers and single crystals indicates the inferior crystal quality of perovskite wafers, which limits the performance and stability of wafer‐based X‐ray detectors. Here, nano‐sized MAPbBr3 powders are utilized to fabricate dense perovskite wafers by low‐temperature hot‐pressing with high transparency above 60% within the 552–800 nm wavelength range. Adjacent nanocrystals assemble following the ordered coalescence mechanism, resulting in the exclusion of nanoscopic pores and crystallographic reorientation. The transparent MAPbBr3 wafer‐based detectors achieve an impressively high X‐ray sensitivity of 1.14 × 105 µC Gyair−1 cm−2 and a low detection limit of 149 nGyair s−1, which is superior to opaque MAPbBr3 wafer detectors (5.64 × 104 µC Gyair−1 cm−2 and 316.7 nGyair s−1) and comparable to MAPbBr3 single‐crystal detectors. Moreover, the detectors demonstrate high uniformity and outstanding stability under continuous X‐ray irradiation of a total dose of up to 5.9 Gyair, equaling to 29 500 times posteroanterior chest examinations. The high sensitivity and low detection limit of the detectors lead to clear X‐ray imaging performance.

Disposable electrochemical sensors based on reduced graphene oxide/polyaniline/poly(alizarin red S)-modified integrated carbon electrodes for the detection of ciprofloxacin in milk.

Anelectrochemical sensor based on an electroactive nanocomposite was designedfor the first timeconsisting of electrochemically reduced graphene oxide (ERGO), polyaniline (PANI), and poly(alizarin red S) (PARS) for ciprofloxacin (CIPF) detection. The ERGO/PANI/PARS-modified screen-printed carbon electrode (SPCE) was constructed through a three-step electrochemical protocol and characterized using FTIR, UV-visible spectroscopy, FESEM, CV, LSV, and EIS. The new electrochemical CIPF sensor demonstrated a low detection limit of 0.0021μM, a broad linear range of 0.01 to 69.8μM, a high sensitivity of 5.09 μA/μM/cm2, and reasonable selectivity and reproducibility. Moreover, the ERGO/PANI/PARS/SPCE was successfully utilized to determine CIPF in milk with good recoveries and relative standard deviation (< 5%), which were close to those with HPLC analysis.

Electrochemical sensing technology based on a ligation-initiated LAMP-assisted CRISPR/Cas12a system for high-specificity detection of EGFR E746–A750 deletion mutation

Epidermal growth factor receptor (EGFR) mutation status is pivotal in predicting the efficacy of tyrosine kinase inhibitor treatments against tumors. Among EGFR mutations, the E746–A750 deletion is particularly common and accurately quantifying it can guide targeted therapies. This study introduces a novel visual sensing technology using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system guided by ligation-initiated loop-mediated isothermal amplification (LAMP) to detect the del E746–A750 mutation in EGFR. Conventional LAMP primers were simplified by designing a pair of target-specific stem-loop DNA probes, enabling selective amplification of the target DNA. The CRISPR/Cas12a system was employed to identify the target nucleic acid and activate Cas12a trans-cleavage activity, thereby enhancing the specificity of the assay. Furthermore, the biosensor utilized high-performance nanomaterials such as triangular gold nanoparticles and graphdiyne, known for their large specific surface area, to enhance sensitivity effectively as a sensing platform. The proposed biosensor demonstrated outstanding specificity, achieving a low detection limit of 17 fM (S/N = 3). Consequently, this innovative strategy not only expands the application scope of CRISPR/Cas12a technology but also introduces a promising approach for clinical diagnostics in modern medicine.

Ultrafast Scintillator Based on Zirconium‐Doped Cesium Zinc Chloride Single Crystals and Their Charge Carrier Dynamics

AbstractScintillators have been widely used for high‐energy radiation imaging. It is in great demand and challenge to develop scintillator materials with fast decay time, high scintillation light yield, and low detection limit for high‐resolution imaging applications such as time‐of‐flight positron emission tomography. However, it is still a challenge to develop the ultrafast carrier dynamics to achieve 100% ultrafast decay time with high scintillation light yield. To meet the demand, a series of component‐tunable Cs2ZnCl4: x%Zr single crystals as promising ultrafast scintillators is successfully developed. With 8% of Zr‐dopant (Cs2ZnCl4: 8%Zr), the single crystal exhibits high light yield (28000 photons MeV−1) and low detection limit (51 nGy s−1) under X‐ray excitation. Photo‐induced transient absorption signals on sub‐nanosecond and nanosecond scales ensure almost 100% fast decay time (≈3 ns) in nanoseconds under γ‐ray excitation. In particular, the different radiative transition processes are achieved under ultraviolet and high‐energy radiation due to the tunable carrier dynamics from the shallow trapped state to the self‐trapped exciton (STE) state.

Defect-Engineered WO3-x Architectures Coupled with Random Forest Algorithm Enables Real-Time Seafood Quality Assessment.

Reliable and real-time monitoring of seafood decay is attracting growing interest for food safety and human health, while it is still a great challenge to accurately identify the released triethylamine (TEA) from the complex volatilome. Herein, defect-engineered WO3-x architectures are presented to design advanced TEA sensors for seafood quality assessment. Benefiting from abundant oxygen vacancies, the obtained WO2.91 sensor exhibits remarkable TEA-sensing performance in terms of higher response (1.9 times), faster response time (2.1 times), lower detection limit (3.2 times), and higher TEA/NH3 selectivity (2.8 times) compared with the air-annealed WO2.96 sensor. Furthermore, the definite WO2.91 sensor demonstrates long-term stability and anti-interference in complex gases, enabling the accurate recognition of TEA during halibut decay (0-48 h). Coupled with the random forest algorithm with 70 estimators, the WO2.91 sensor enables accurate prediction of halibut storage with an accuracy of 95%. This work not only provides deep insights into improving gas-sensing performance by defect engineering but also offers a rational solution for reliably assessing seafood quality.

Colorimetric aptasensor based on self-screened aptamers and cascaded catalytic reaction for the detection of quarantine plant bacteria

Quarantine plant bacteria (QPB) are significant component of invasive alien species that result in substantial economic losses and serious environmental damage. Herein, a colorimetric aptasensor has been proposed based on the sandwich structure and the cascaded catalytic strategy for on-site detecting Xanthomonas hyacinthi, a type of QPB, in natural environments. The self-screened aptamer obtained through SELEX can bind to specific sites on the surface of viable organism with high affinity and specificity, which guarantees the selectivity of aptasensor. As an important part of the aptasensor, MIL-88-NH2(Fe) not only acts as a multifunctional carrier for both aptamers and glucose oxidase, but also catalyzes enzyme-like reaction because of specific surface area, amino and peroxidase-like activity. The present of Xanthomonas hyacinthi can trigger the formation of a sandwich structure and the occurrence of cascade catalytic reaction, enabling the detection with UV–Vis spectra and naked eyes. The proposed aptasensor presents a low detection limit of 2 cfu/mL and a wide linear range of 10 -107 cfu/mL. Compared to traditional detection methods for QPB, the reasonable design, high selectivity and convenience significantly improve the detection efficiency and contribute to environmental protection.

New method for rapid detection and simultaneous determination of prohibited adrenergic drugs in sports using thin layer chromatography and image processing

This study presents a new method for simultaneous determination of fenoterol, procaterol, clenbuterol, terbutaline and isoprenaline drugs using thin-layer chromatography assisted by image analysis (HPTLC-IA) combined with sensitive detection modes. Separation of the selected drugs was obtained using HPTLC Silica gel 60 F254 plates and mixture of isopropyl alcohol, ethyl acetate and ammonia (20:25:1.5 v/v/v) as mobile phase. UV detection mode combined with the use of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS+•) radicals were applied for the first time for sensible detection of the separated drugs. Grey, green, red and blue scales respectively were selected for image analysis and accurate quantification of spot area. According to the obtained result, the use of DPPH• and ABTS+• reagents revealed a higher sensitivity in detection and accurate quantification of the analyzed drugs. Lower limit of detection (LOD = 0.07 ± 0.03 µg/spot; 0.09 ± 0.03 µg/spot and 0.10 ± 0.04 µg/spot) was obtained for fenoterol, procaterol and terbutaline using the ABTS+• radical and red scale selection for image processing. With the exception of isoprenaline, the proposed HPTLC-IA method showed a good separation of fenoterol, procaterol, clenbuterol and terbutaline drugs from other compounds in urine samples. Good recovery was obtained for these drugs in spiked urine samples.

Magnetically-manipulatable and self-cleaning lab-on-a-bubble Ag@TiO2@Fe3O4 hollow spheres and their application in SERS detection and photocatalytic degradation

In this study, an innovative highly sensitive self-cleaning Lab-on-a-bubble SERS substrate: Ag NPs@TiO2@Fe3O4 hollow spheres, was developed using spray pyrolysis and following depositions. The reusable and magnetically controllable substrate consists of iron oxide hollow spheres (Fe3O4) covered by a TiO2 shell and silver nanoparticles (Ag NPs) deposited on the outer surface. Experimental results show that this new composite SERS substrate exhibited a low limit of detection (LOD) down to 10-10M, and the enhancement factor (EF) reached 1.01 X107. It suffered only 3 % loss after 10 repeatable recycle tests if the self-cleaning under UV was performed after each test. This novel substrate possessed good photocatalytic degradation ability for methylene blue (MB) dyes even under visible light. In that case, MB can be dissociated up to 99.8 % and the reaction rate constant (K) reached 0.0271/min. Taking advantage of this, the feasibility of reusable SERS detection through self-cleaning under visible light was also demonstrated.

A ratiometric fluorescent probe for selective detection of hypochlorite (ClO−) and gallium (III) (Ga3+) ions in environmental and food samples

Hypochlorite (ClO−) and gallium (Ⅲ) ions (Ga3+) have extensive applications in various human industries and daily activities. However, their inherent toxicity poses significant risks to environmental preservation and human well-being. Hence, the development of reliable and handy detection tools for ClO− and Ga3+ in the environment and food is crucial. In this study, a ratiometric fluorescent probe was prepared based on benzothiazolaldehyde and pyridine-2-carboxylic acid hydrazide, which exhibited exceptional performance characteristics for the selective detection of ClO− and Ga3+. These features include high specificity, low detection limits (0.28 μM for ClO−, 0.13 μM for Ga3+), mild pH conditions (pH 4–11 for ClO−, pH 6–11 for Ga3+), fast response time (within 30 s), as well as versatile applicability across different matrices such as water, soil, food, and plant samples. Additionally, this probe can be used with a smartphone color recognition app. The probe offers a convenient and effective tool for the detection of ClO− and Ga3+, demonstrating its potential application value in environmental monitoring and food safety.

Bismuth oxide nanoparticles enabled sensitive electrochemical determination of Vitamin B12

The intake and monitoring of vitamin B12 levels are highly essential to maintain a healthy body condition. Herein, we report bismuth oxide nanoparticles (Bi2O3 NPs) facilitated electrochemical sensing of vitamin B12 with admirable sensitivity, selectivity, and low detection limit. A facile hydrothermal method was adopted for synthesizing Bi2O3 NPs and their physico-chemical properties were analyzed using various techniques. The electrochemical sensing of vitamin B12 was validated through cyclic voltammetry and differential pulse voltammetry techniques. The sensing results illustrate that the modified electrode exhibits a wide linear range between 10 nM and 275 nM with a low detection limit value of 3.66 nM. In addition to the enhanced electrocatalytic behavior, the Bi2O3 NPs have also enabled the discrimination of vitamin B12 from various potential interfering molecules (even adding a 5-fold concentration). The developed sensor also exhibits better stability, selectivity, and reproducibility towards the target analyte. Finally, the fabricated electrode was applied for the determination of vitamin B12 in serum, and plasma samples, and the results were highly satisfactory with recovery values of 98.4 % to 99.8 %.

Prepared Sandwich structure WS2/ag@MIP composite for ultrasensitive SERS detection of trace 17β-estradiol in food

17β-E2 is used in animal growth regulation and agricultural fertilizer, and even ng L−1 mass concentration levels can show biological effects. In this work, Ag NPs was used as surface-enhanced Raman spectroscopy (SERS) source and WS2 was synthesized by a simple method to provide a uniform distribution platform for Ag NPs. The MIP was the shell, which can selectively enrich the target molecule, pull the distance between the target molecule and SERS source, and protect Ag NPs. A cyclable SERS substrate with high sensitivity for detecting 17β-E2 in food was constructed. The optimized WS2/Ag@MIP as SERS substrate has the advantages of high Enhanced Factor (EF = 2.78 × 109), low detection limit (LOD = 0. 0958 pM), strong anti-interference ability, and good recycling performance. Moreover, the detection of 17β-E2 in real samples still has good accuracy. This work provides a new possibility for the trace detection of 17β-E2 in food.