The benefits of moderate-to-vigorous physical activity (MVPA) are well known, however objective measurement of MVPA still poses challenges on a large-scale level, especially in terms of cost. Validation of low-cost accelerometers for MVPA assessment is required prior to population surveillance projects. Examples of these recently available accelerometers include the New Lifestyles NL-1000 (NL; New Lifestyles Inc., Lee's Summit, Missouri, USA, $48), an accelerometer with a modifiable MVPA intensity threshold, and the Omron HJ-151 (OM; OMRON Healthcare, Inc., Bannockburn, IL, $13), a piezoelectric pedometer which outputs MVPA based on estimated walking speed (i.e., modeled from steps/min and participant height). The ActiGraph (AG; ActiGraph LLC, Pensacola, FL, $325), is an accepted research grade accelerometer with validated cut points used to determine time spent in MVPA. PURPOSE: This purpose of this study was to compare the outputs of time spent in MVPA from NL and OM with that of the AG in free-living situations. METHODS: Participants (n=26; females=17; Age=27.3 ± 7.1, range=18-44; BMI=23.8 ± 2.8) wore the three instruments concurrently during waking hours (except for water activities) for one day (11.4 ± 2.0 hours). The NL MVPA intensity range was set to the manufacturers' default setting. Time in MVPA from the NL and OM were recorded by participants at day end. AG minute-by-minute activity counts were downloaded and converted using a validated cut point (MVPA=1952 counts/min). Descriptive statistics, percent difference, repeated measures analysis of variance, and effect size (ES) were used to compare instrument outputs. Bland-Altman plots were used to assess systematic bias between the instruments. RESULTS: Detected time in MVPA for NL, OM, and AG were 44.6 ± 22.4, 38.0 ± 19.7, and 48.0 ± 20.6 min, respectively. The difference between OM vs. AG MVPA (Ä=10.0 min) was significant (p<.001, ES=0.49). All other comparisons were not significant. Bland-Altman plots displayed no evidence of systematic bias. CONCLUSIONS: In comparison to the AG, the NL detected similar time in MVPA while the OM detected significantly lower time in MVPA. These data support the NL as a low cost alternative to the more costly AG for detection of MVPA in free-living adults, especially for population monitoring.