Low Nitrogen Content Research Articles

Current Status and Promotional Strategies of Millets: A Review

Millets or nutri-cereals are high-energy foods, its domestication and cultivation date back at least 10,000 years. Millets are typically grown on degraded, marginal terrain that receives little rainfall and has low soil nitrogen concentration. Finger millet, Pearl millet, Foxtail millet, Barnyard millet, Proso millet, Kodo millet, and Tiny millet are the seven major millets grown around the world. In the case of India, millet output peaked in the 1980s and then steadily fell as a result of a substantial fall in the area that was being grown. The abundance of dietary fibres, antioxidants, minerals, phytochemicals, polyphenols, and proteins in millets makes them special in the fight against diseases. Millet is currently addressing an essential field of research for food scientists because to their considerable engagement in nutritional security and potential growing health consequences. Millets' nutritional value can be further enhanced by using appropriate and efficient processing techniques. Given the numerous health advantages and environmental benefits of millets, it is urgently necessary to create millet-based government policies that acknowledge their contributions to achieving nutritional security and bring them back into agricultural production in order to create cropping systems that are climate resilient.

Effect of magnetic fields on simultaneous nitrification and denitrification microbial systems.

Magnetic fields positively influence the nitrogen removal efficiency in activated sludge systems. However, the structural succession pattern of microorganisms by magnetic fields still remains further explored. In this paper, a magnetic simultaneous nitrification and denitrification (MSND) reactor was constructed, and the influence of optimized magnetic field intensity (0, 10, 20 and 30 mT) on the nitrogen removal efficiency was investigated at HRT 6 h, 28.0-30.0 °C, and pH 7.0-8.0. Molecular biology was used to investigate the succession process of the dominant microbial flora and the functional gene structure of MSND systems. The results showed that the denitrification effects of the MSND system were significantly enhanced, which contributed to the lower concentration of total nitrogen in the effluent of the magnetic reactor than that of the nonmagnetic group reactor. The magnetic fields induced the succession of microbial community structure and improved the stability of microbial communities, thereby the relative abundances of nitrifying and denitrifying bacteria, and the functional genes were improved. In particular, the abundance of functional genes related to gene proliferation and transmembrane transport was increased. Therefore, the efficient nitrogen removal was achieved, which gives inspiration in the enhanced wastewater treatment by magnetic fields.

Impact of Nitridation on Bias Temperature Instability and Hard Breakdown Characteristics of SiON MOSFETs.

We study how nitridation, applied to SiON gate layers, impacts the reliability of planar metal-oxide-semiconductor field effect transistors (MOSFETs) subjected to negative and positive bias temperature instability (N/PBTI) as well as hard breakdown (HBD) characteristics of these devices. Experimental data demonstrate that p-channel transistors with SiON layers characterized by a higher nitrogen concentration have poorer NBTI reliability compared to their counterparts with a lower nitrogen content, while PBTI in n-channel devices is negligibly weak in all samples independently of the nitrogen concentration. The Weibull distribution of HBD fields extracted from experimental data in devices with a higher N density are shifted towards lower values with respect to that measured in MOSFETs, and SiON films have a lower nitrogen concentration. Based on these findings, we conclude that a higher nitrogen concentration results in the aggravation of BTI robustness and HBD characteristics.

FAME properties, bio-oil productivity and carbon yield coefficient of Chlorella sorokiniana grown with low and high initial nitrogen concentrations

In this work the effect of the initial nitrogen concentration on the fatty acid methyl ester (FAME) properties namely the saponification number (SN), the iodine value (IV), the cetane number (CN) and the higher heating value (HHV) of bio-oil produced by Chlorella sorokiniana was examined. Also, the lipid and protein content of the biomass of C. sorokiniana and the biomass yield were measured. Anaerobic digestate and glycerol were used to formulate the culture media. The initial concentration of atomic nitrogen varied in the four cultivations and it was equal to 108.2 mg/l, 300 mg/l, 800 mg/l and 2000 mg/l while the initial concentration of carbon was held constant and equal to 13.99 ± 0.1 g/l. As the initial nitrogen concentration was increased, the lipid content decreased while the protein content and the biomass yield increased. Fatty acids (FA) from C10:0 to C26:1 were produced, however, the fatty acids that were found in the highest percentage were C16:0, C16:1, C18:1 and C18: 2. With respect to saturation, monosaturated FA were predominant in all treatments constituting from 55.9% to 59.4% of the total FA, while significant percentages of saturated FA were produced ranging from 24.5% to about 31.1%. With respect to the chain length, medium chain FA predominate constituting from 59.5% to 81.3% of the total FA. The four measured FAME properties, with the exception of the cetane number (CN) for the No = 2000 mg, were within acceptable limits. It was shown that when the nitrogen concentration in the medium increases, while the carbon concentration remains constant, the biomass carbon yield coefficient increases substantially.

Camellia japonica Flowers as a Source of Nutritional and Bioactive Compounds.

In recent decades, plants have strengthened their relevance as sources of molecules potentially beneficial for health. This underpinning effect also arises from the extensive research that has been conducted on plants that are typically undervalued, besides being scarcely used. This is the case with Camellia japonica in Galicia (NW Spain), where, despite its abundance, it is exclusively used for ornamental purposes and has been studied only for its proximate composition. Thus, the present study was conducted on several additional parameters in the flowers of eight C. japonica varieties. Our results show that camellia has a high nutritional value, with carbohydrates as the most abundant macronutrients followed by a moderate protein content (4.4-6.3 g/100 g dry weight) and high levels of polyunsaturated fatty acids (especially ω-3 fatty acids, which represent 12.9-22.7% of the total fatty acids), raising its potential for use for nutritional purposes. According to the thermochemical characterization and elemental composition of camellia, the raw material has poor mineralization and low nitrogen content, but high percentages of volatile matter and high carbon-fixation rates, making it a promising alternative for biofuel production. Furthermore, preliminary analysis reveals a high concentration of different bioactive compounds. As a result of these findings, camellias can be used as food or functional ingredients to improve the nutritional quality of food formulations.

The contribution of epiphytic microbiota in oat and Italian ryegrass to silage fermentation products and bacterial community structure of whole-crop maize

BackgroundThe purpose of this research is to study the contribution of epiphytic microbiota in fresh oat (OT), Italian ryegrass (IR) and whole-crop maize (MZ) to silage fermentation products and bacterial community structure of MZ. After γ-ray irradiation, the sterile MZ was treated via microbiota transplantation method: (1) sterile deionized water (STMZ); (2) microbiota epiphytic on MZ (MZMZ); (3) microbiota epiphytic on OT (MZOT); (4) microbiota epiphytic on IR (MZIR). Triplicate silos of each treatment were tested after 1, 3, 7, 15, 30 and 60 days of ensiling.ResultsMZMZ had higher (P < 0.05) lactic acid contents, and lower (P < 0.05) ammonia nitrogen and ethanol contents than MZIR and MZOT on day 60. The relative abundance of Lactobacillus in MZMZ decreased from 84.0% on day 3 to 44.7% on day 60. MZMZ had higher (P < 0.05) abundances of ‘Nucleotide metabolism’, ‘Replication and repair’ and ‘Membrane transport’, and lower (P < 0.05) abundance of ‘Amino acid metabolism’ than MZOT and MZIR on day 3.ConclusionsThe silage fermentation products of MZ were highly affected by the activity and compositions of epiphytic microbiota. The Enterobacteriaceae, Hafnia-Obesumbacterium, hetero-fermentative and acid-resistant Lactobacillus took primary responsibility for the high dry matter loss and ethanol contents and low lactic acid contents in MZ silage.Graphical

VARIABILITAS KANDUNGAN C-ORGANIK PADA TANAH ULTISOL YANG DIBERI BERBAGAI JENIS BAHAN ORGANIK UNTUK PERTUMBUHAN TANAMAN SAWI

Ultisol is a potential acid mineral soil for the development of agricultural crops with an area of 45.8 million hectares, but has problems of soil acidity, low content of C-Organic, nitrogen, phosphate and potassium, but this can be overcome by applying fertilizers. This study aims to determine the effect of various types of organic matter on C-Organic and the growth of mustard greens. This research was conducted on Field II of the Faculty of Agriculture, and sample analysis was carried out at UPT. Halu Oleo University Integrated Laboratory.The study used a randomized block design (RBD) consisting of 9 treatments, namely TB = without organic matter, SI = cow manure 75 g polybag-1, SII = cow manure 150 g polybag-1, AI = chicken manure 75 g polybag -1, AII = chicken manure 150 g polybag-1 , KI = goat manure 75 g polybag-1 , KII = goat manure 150 g polybag-1 , HI = compost vegetation komba-komba 75 g polybag-1 , HII = komba-komba vegetation compost 150 g polybag-1.The results showed that the provision of various types of organic matter was 0.62%. As for the results after administration of various types of organic matter with various concentrations continued to decrease successively in the treatment TB, SI, SII, AI, AII, KI, KII, HI, namely 0.26%, 0.28%, 0, 32%, 0.34%, 0.40%, 0.52%, 0.54%, 0.60%, and after that there was an increase in the HII treatment, 0.78%, with a dose of 150 grams. Application of cow manure, chicken coop, goat coop, and komba-komba vegetation compost had an effect on the height of the mustard plants. Keywords : C-Organic, Mustard Plant, Ultisol.

Genetic analysis of acyl-CoA carboxylases involved in lipid accumulation in Rhodococcus jostii RHA1.

In actinomycetes, the acyl-CoA carboxylases, including the so-called acetyl-CoA carboxylases (ACCs), are biotin-dependent enzymes that exhibit broad substrate specificity and diverse domain and subunit arrangements. Bioinformatic analyses of the Rhodococcus jostii RHA1 genome found that this microorganism contains a vast arrange of putative acyl-CoA carboxylases domains and subunits. From the thirteen putative carboxyltransferase domains, only the carboxyltransferase subunit RO01202 and the carboxyltransferase domain present in the multidomain protein RO04222 are highly similar to well-known essential ACC subunits from other actinobacteria. Mutant strains in each of these genes showed that none of these enzymes is essential for R. jostii growth in rich or in minimal media with high nitrogen concentration, presumably because of their partial overlapping activities. A mutant strain in the ro04222 gene showed a decrease in triacylglycerol and mycolic acids accumulation in rich and minimal medium, highlighting the relevance of this multidomain ACC in the biosynthesis of these lipids. On the other hand, RO01202, a carboxyltransferase domain of a putative ACC complex, whose biotin carboxylase and biotin carboxyl carrier protein domain were not yet identified, was found to be essential for R. jostii growth only in minimal medium with low nitrogen concentration. The results of this study have identified a new component of the TAG-accumulating machinery in the oleaginous R. jostii RHA1. While non-essential for growth and TAG biosynthesis in RHA1, the activity of RO04222 significantly contributes to lipogenesis during single-cell oil production. Furthermore, this study highlights the high functional diversity of ACCs in actinobacteria, particularly regarding their essentiality under different environmental conditions. KEY POINTS: • R. jostii possess a remarkable heterogeneity in their acyl-carboxylase complexes. • RO04222 is a multidomain acetyl-CoA carboxylase involved in lipid accumulation. • RO01202 is an essential carboxyltransferase only at low nitrogen conditions.

The relationship of nitrogen nutrition conditions with the productivity of Tamasha potatoes

The article presents the results of three-year (2015-2017) studies research on the influence of nitrogen nutrition conditions on the productivity and quality of potatoes variety Tamasha. The studies were carried out on dark brown heavy loamy carbonate soils of Central Kazakhstan with a humus content of 2.73-2.79 %, total nitrogen - 0.147-0.172 %, total phosphorus - 0.20-0.25 %, with a high level of availability of mobile phosphorus and potassium, low content of nitrate nitrogen, slightly alkaline reaction of soil solution. Studies have shown a high responsiveness of potatoes to nitrogen fertilizers. According to the results of the research, a high correlation between potato productivity and the nitrate nitrogen content in the soil (R=0.85-0.96) was revealed, the optimal level of nitrate nitrogen in the 0-40 cm soil layer was determined for Tamasha potatoes - 17-19 mg/kg. The increased nitrogen content of nitrates in the soil slightly reduced the starchiness of potatoes.

Nitrogen functionalities in mesoporous carbons and their impact on the sulfur infiltration efficiency and in the electrochemical performance of Li[sbnd]S cells

The cyclability of LiS batteries depends strongly on electrochemical cell stability, which is affected by the dilution of lithium polysulfides in the electrolyte. One of the proposed solutions is the use of porous carbonaceous materials in the cathode that trap lithium polysulfide species and prevent them from reaching the electrolyte. This is possible by using dopant heteroatoms in the carbon framework such as nitrogen to generate anchoring sites reducing the shuttle effect. To understand how the sulfur infiltration process in carbonaceous materials is affected by the inclusion of nitrogen in the carbon structure and its effect on the electrochemical performance of LiS batteries, in this study N-doped mesoporous carbons were prepared by varying the carbonization temperature, focusing on the structural and compositional variations. At the highest carbonization temperature (850 °C), a carbonaceous material with the lowest nitrogen content was obtained mainly prevailing the N-quaternary functionality over N-pyrrolic and N-pyridinic. The sulfur infiltration into the pores of the material was improved due to the moderate polarity obtained under the carbonization temperature used (850 °C). Given the distribution of nitrogen functionalities, it was possible to obtain a material with a high degree of graphitization reflected in the electrochemical performance where after 100 cycles a discharge capacity of 422.3 mA h g−1 was reached with a Coulombic efficiency of 99%.

Utilization of hemp stalk as a potential resource for bioenergy

Recent concerns regarding climate change and rising energy costs have dramatically increased interest in using alternative energies, especially biomass energy which is carbon neutral. Hemp is among the fastest-growing plants with unique fiber characteristics. The objective of this study was to investigate the physical and chemical properties of hemp stalks of seven different clones and to assess their feasibility as a sustainable bioenergy resource. Seven clones (KU03, KU18, KU27, KU45, KU49, RPF1, and RPF2) of four-month-old hemp (Cannabis sativa) were used in this work. Physical properties, volatile content, fixed carbon, ash content, calorific value, chemical composition, ash composition, and metal element of the samples were investigated. The results revealed that hemp stalk had desirable fuel characteristics with high volatile substance, high heating value, low ash content, very low nitrogen content, and non-detectable sulfur. Selecting well-adapted clones and appropriate technology which can convert the hemp stalks to suitable bioenergy forms are important aspects of bioresource management. Based on our findings, some selected hemp clone biomass possessed excellent characteristics and great potential to be used as raw material for bioenergy production.

PRODUCTION ENVIRONMENTALLY FRIENDLY DIESEL FUEL BASED ON DEAROMATIZED DIESEL FRACTION AND BUTYL ESTERS OF FATTY ACIDS OF SUNFLOWER OIL

Currently, special attention is paid to the environmental performance of fuels, because, the ever-increasing quantity of transport leads to an increase in the emission of harmful substances into the atmosphere. In particular, for the introduction of environmentally friendly technologies for the production of diesel fuels, first of all, it is necessary to obtain diesel fuel with a low content of sulfur, nitrogen and polycyclic aromatic compounds, which requires the processes of hydrotreating, hydrocracking and hydrodearomatization are carried out, which require significant economic costs. The presented article considers an alternative process for reducing the content of harmful substances, in particular aromatic compounds in the composition of diesel fuel. The process of dearomatization of the diesel fraction was carried out using N-methylpyrrolidone as an extractant at a ratio of extractant : diesel fraction 2:1 for 6 hours. The physicochemical properties of the dearomatized diesel fraction have been studied. In the resulting dearomatized diesel fraction, the sulfur content decreased to 0.0220%, and the content of aromatic compounds to 0%, in addition, the fractional composition is facilitated, the density and viscosity decrease. In order to replenish the resource of diesel fuel, oxygen-containing additives were added to the dearomatized diesel fraction - butyl esters of fatty acids of sunflower oil in an quantity of 5-10%, and the physicochemical properties of the resulting compounds were studied. It was revealed that the addition of butyl esters of fatty acids of sunflower oil to the composition of the dearomatized diesel fraction favorably affects the quality indicators of the obtained compounds. So, there is an increase in the flash point by 6°C and the cetane number by 4-5 points, and the sulfur content decreases to 0.0198%. In addition, the diameter of the wear scar decreases by about 2 times and for a 5% compound it is 0.430 mm, and for a 10% compound it is 0.370 mm, which meets the modern requirements of the standard. Thus, obtaining environmentally friendly diesel fuel is possible by dearomatization of the diesel fraction with the subsequent addition of butyl esters of fatty acids of sunflower oil, which can be recommended as a multifunctional additive to diesel fuels.

Structural Evolution of 10% Cr–3% Co Steel Microalloyed with Re and Cu during Creep Au 923 К

Structural evolution of the tempered lath martensite of the 10% Cr--3% Co steel microalloyed with rhenium and copper with a low nitrogen content and a high boron content during creep at 923 K was investigated for the purpose of establishment of the rison of decrease in creep resistance of this steel under the low applied stress. The tempered martensite lath structure of 10%Cr-3%Co steel with an average lath size of 370 nm and a high dislocation number density of 2 ×1014 m–2 was observed after normalizing at 1323 K with the following tempering at 1043 K for 3 h. The structure was stabilized by M23C6 carbides, M6C carbides, and NbX carbonitrides. During long-term creep, the lath structure strongly experienced an evolution: the width of the martensitic laths increased significantly, dislocation density decreased, the Laves phase and Cu-enriched particles remarkably coarsen. Such structural evolution correlates with an appearance of creep strength breakdown on curves “Applied stress vs. Time to failure” and “Minimum creep rate vs. Applied stress”. Significant coarsening of the Laves phase particles and Cuenriched particles via formation of the large particles with sizes more than 250 nm along high-angle boundaries and full dissolution of the fine particles with sizes less than 50 nm along low-angle boundaries of martensite laths is considered to be the main cause of degradation of the creep resistance of the steel studied.

Antibiotics Removal via Novel N-Doped Carbon Derived from Carbonization of Different Forms of Polyaniline

N-doped carbon (CP) was synthesized through carbonization of a polyaniline (PANI) prepared using interfacial polymerization. Different forms of PANI, such as emeraldine salt (ES), emeraldine base (EB), and aniline oligomers, were carbonized at different temperatures (700°C–1,000°C) in the presence of the N2 atmosphere to form CPs (CP7–CP10). CP prepared under different conditions was applied as adsorbent for antibiotics. It was observed that ES-PANI carbonized at 1,000°C formed graphitic N-doped carbon (CP10) and showed better adsorption of metronidazole (MET) compared with other CPs. The adsorption results showed that CP10 derived from the ES-form of PANI (PA1) achieved ∼97% MET removal within 5 min following pseudo-second-order kinetics (k = 0.347 g mg−1 min−1). CP10 has a high specific surface area (Sa) of 800.65 m2 g−1 and high graphitic nitrogen content (52.26% of total N) responsible for the rapid adsorption of the antibiotics through π-π electron acceptor-donor interaction, hydrogen bonding, and electrostatic attraction. In addition, CP10 has an adsorption capacity of 200.8 mg g−1 for MET. The highest activity of CP10 was due to high graphitic nitrogen content and high Sa. However, CPs formed from EB-PANI and aniline oligomers did not perform efficiently due to low Sa and graphitic nitrogen content. Similarly, CP10 applied for the adsorption of the ciprofloxacin (CIP) and tetracycline (TET) achieved maximum adsorption capacity of 344.8 and 263.2 mg g−1, respectively. Therefore, CP10 is a promising adsorbent for removing emerging pollutants from an aqueous solution.

Organic amendment effects on cropland soil organic carbon and its implications: A global synthesis

Increasing soil organic carbon (SOC) content can promote soil health and crop yield. Organic amendment application is a common practice to accelerate soil carbon sequestration in cropland. The effects of different organic amendments (e.g., compost, slurry) on SOC are uncertain. Previous studies have investigated SOC response to environmental factors but not well addressed the effect of organic amendment characteristics (e.g., source, liquid/solid form, pH). Depending on 1,972 comparisons from 424 articles, we undertook a meta-analysis approach to explore organic amendment effects on SOC content and the related influence factors. The results show that organic amendment addition increased SOC by an average of 26.9% (or 5.1 Mg C ha−1). Specifically, organic amendments can enhance SOC over the long term (>20 years) or within 1.0 m depth. Organic-residue inputs increased more SOC in regions with an arid climate, alkaline soils, or no-mineral nitrogen application soils than the humid climate regions, acidic soils, and nitrogen application soils, respectively. Sewage sludge and municipal solid waste as organic amendments led to high SOC. The organic amendments' pH and liquid/solid form caused significant differences in SOC accumulation. Amendments with a relatively low carbon content or rich nitrogen content have great potential to increase SOC storage. Our results illustrate the importance of amendment characteristics when estimating the potential of organic amendment addition to soil carbon accumulation. This study highlights that amendment application might be a sustainable strategy to increase soil carbon storage while promoting organic waste utilization.

Influence of Epigallocatechin Gallate on Quality of Cooked Harpiosquillid Mantis Shrimp (Harpiosquilla raphidea) Subjected to Multiple Freeze-Thaw Cycles

Effects of epigallocatechin gallate (EGCG) at 200 or 400 ppm on quality changes of cooked Harpiosquillid mantis shrimp (Harpiosquilla raphidea) (CHMS) meat subjected to varying freeze-thaw cycles (F-T-C; 0, 1, 3, and 5) were studied. With augmenting F-T-C, the CHMS meat underwent deterioration to higher extent as indicated by upsurges in exudate loss, tougher texture along with increased microbial counts. The addition of EGCG could retard those changes in a dose-dependent manner. Furthermore, EGCG prevented microbial growth as ascertained by lower counts and total volatile basic nitrogen (TVB) content. Lipid oxidation was also impeded in the presence of EGCG, especially at a high concentration as evidenced by lowered malondialdehyde (MDA), while maintaining polyunsaturated fatty acids, particularly EPA and DHA when F-T-C raised. Total disulfide bond content and surface hydrophobicity of CHMS added with EGCG were lower than those of the control. Those alterations were more retarded as EGCG concentration increased. Therefore EGCG at 400 ppm effectively prevented quality loss of CHMS meat during repeated F-T process.

Responses of Worowo [Senecio biafrae (Oliv. &amp; Hiern.) S. Moore] to Composts enriched with Organic Nitrogen Sources

Worowo (Senecio biafrae) is a semi-wild, indigenous and nutritious vegetable. Demand for worowo vegetable is increasing due to its nutritional values, thus necessitating its domestication for increased production. However, there is limited information on soil fertility requirements of worowo and the use of inorganic fertilizers could be expensive and detrimental to environment. Composts, on the other hand are environment-friendly and effective, but their use is constrained by low nitrogen contents, thereby necessitating enrichment with various nitrogen sources. Responses of worowo to organically enriched composts were therefore investigated. Composted Cattle Dung+Sawdust (CDS) and Poultry Droppings+Sawdust (PDS), at 1:1 (w/w) were enriched to 60 g N/ kg with meals from dried bone-BnM, blood-BM, hoof-HM, and horn-HnM, neem-NM and Tithonia leaves-TM. Compost treatments obtained were: CDS and PDS (no enrichment added), CDSBnM, CDSBM, CDSHM, CDSHnM, CDSNM and CDSTM; PDSBnM, PDSBM, PDSHM, PDSHnM, PDSNM and PDSTM. Worowo was raised in pots with CDS, PDS and 60 g N/kg enriched composts at 30 t/ha and control (soil alone), compared with 60 kg N/ha NPK 15-15-15 in a completely randomized design with three replicates. Data generated from the growth indices and edible Shoot Yield (ESY) of worowo (t/ ha), which were measured at 60, 120 and 180 Days After Planting (DAP) were analyzed using ANOVA at α0.05. ¬¬¬¬¬¬The ESY (t/ha) of 60 g N/kg CDSNM (54.93) was significantly higher than NPK (36.53) and others but similar to CDS (50.93) while pots treated with PDS were lowest (8.00). The CDSDNM at 60 g N/kg applied at 30 t/ha could hence be adopted for worowo production.

HYDROCHEMISTRY OF THE ZEYA RESERVOIR IN HIGH-WATER YEARS

The results of studying the chemical composition of the waters of the Zeya reservoir in high-water years (2013, 2021) are presented. It is shown that a signifi cant infl ow of water into the reservoir during these years caused in its waters a uniform distribution of concentrations of dissolved substances in the water area and depth in the summer- autumn period and extremely uneven in winter. The maximum values of mineralization, concentrations of calcium, magnesium, bicarbonate and sulfate ions, ammonium nitrogen, organic substances and dissolved iron were established in winter in the estuarine areas of the Argi and Zey regions as a result of the infl uence of cryogenic processes and fl ooding of the marshes of the Upper Zey plain. In the dam and canyon parts of the reservoir in winter, there were no large diff erences in mineralization values (28,4–41,9 mg/dm3), low content of ammonium nitrogen (&lt; 0,03 mg N/dm3), increased values of water color (62–87°), concentrations of dissolved iron (0,35–0,74 mg/dm3) and organic matter (10,0–13,3 mg O/dm3). An assumption is made about the receipt of large amounts of dissolved substances in the Amur River in winter due to increased water consumption of the Zeyskaya HPP

Effect of Marinating Temperature of Atlantic Herring on Meat Ripening, Peptide Fractions Proportion, and Antioxidant Activity of Meat and Brine

The temperature has a significant effect on cathepsin activity, but the effect of temperature on the ripening of marinades, and the formation of protein hydrolysis products, is less studied than other technological factors. The results of this study showed that herring marinated at 2 °C showed a higher mass yield, but lower non-protein nitrogen (NPN), peptides, and free amino acid fraction content, than after marinating at 7 and 12 °C. The higher temperature increased the free amino acid content the most, and decreased the hardness, as measured via sensory assessment, of the marinated meat. This was confirmed by the hardness measurement in the texture profile analysis. The highest activity of cathepsins D and B in the meat was found at 7 °C, while cathepsin L was found at 2 °C. Increasing the temperature by 10 °C increased the diffusion/loss of nitrogenous substances from the meat to the brine by 36%. The meat and brine showed high antioxidant activity, which depended on the marinating temperature, and originated mainly from the 5–10 or &lt;5 kDa fraction. The meat had a higher ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonate) activity than the brine, opposite to the DPPH (2,2-diphenyl-1-picrylhydrazyl radical) activity, while the FRAP (Ferric Reducing Antioxidant Power) capacity was similar for meat and brine. The fractionation of the meat and brine extracts increased the antioxidant potential of FRAP and ABTS only for the brine. The most hydrophobic peptides were released during marinating at 7 °C. The meat and brine were dominated by 2–4 kDa peptides, followed by 4–6 and 0.5–2 kDa. The higher temperature favored a higher proportion of &lt;4 kDa than &gt;4 kDa peptides in the brine.

The landscape position of the Roman period cemetery and pedological meaning of its cultural layer at the Malbork-Wielbark archaeological site (northern Poland)

Cultural layers are soil-sedimentary formations transformed by human activity. Unlike other anthropogenic soils/horizons, the cultural layers representing ancient cemeteries at archaeological sites are rather rarely studied. This research characterises the cultural layer of the eponymous cemetery of the Wielbark culture related to the Goths, dated to the Roman period (BC/AD) and located in northern Poland. The cemetery was founded in the edge zone of a morainic plateau and the delta of the Vistula river, on a small inland dune hillock, forming the least fertile habitat in the area. The necropolis covers more than 2200 burials. It was used continuously for about 600 years and preceded by a pre-Roman settlement phase. In the stratigraphy, human activity is recorded as a cultural layer, covering relics of the primary soil, which is a Podzol developed from quartz sand. The cultural layer is buried under younger colluvial and aeolian sediments reaching a thickness of 2.2 m. Radiocarbon and dendrochronological datings confirm the layer was formed between 2555 ± 35 BP and 1590 ± 30 BP. The pedological features of the Wielbark cultural layer are unlike those of other types of anthropogenic soil/horizon. It is characterised by an extremely sandy texture, dark grey to black colour, and low organic carbon, nitrogen and phosphorus contents. The black colouration relates to accumulation of pyrogenic black carbon originating from specific burial rituals of the Wielbark people. Classification of the Wielbark cultural layer according to the WRB soil classification seems problematic: it does not fit the criteria of any anthropogenic or natural surface diagnostic horizon. Its specificity may be marked only by the use of the Pyric supplementary qualifier. In terms of traditional pedological nomenclature, the described Wielbark-type cultural layer may be considered as ‘archaeo-necrosol’.