Production Losses Research Articles

Changes in phycospheric and environmental microbes in Neoporphyra haitanensis during the cultivation cycle

Neoporphyra haitanensis is an economic seaweed widely cultivated along the coast of China, allowing 4–5 times harvests during a cultivation season. The composition and functions of algal-microbial communities are intricate, dynamic, and closely linked to the health of algae. This study utilized metagenomic and 16S ribosomal RNA (rRNA) sequencing to investigate the dynamic changes in the phycospheric microbial communities of Neoporphyra haitanensis (PMC-Nh) and bacterial communities of surrounding seawater (BC-SW) during the cultivation cycle, providing valuable reference data for monitoring and preventing diseases of cultivated seaweed. The results indicated that N. haitanensis might possess the ability to “recruit” and “select” surrounding microbial communities. Beneficial bacteria contributing to carbon, nitrogen, sulfur or phosphorus cycles, such as Proteobacteria, Firmicutes, Cyanobacteria at the class level, and Rhodobacteraceae, Saprospiraceae, Flavobacteriaceae at the family level, exhibited increased abundance. A symbiotic relationship based on resource supply formed between algae and PMC-Nh. However, potential pathogenic bacteria capable of degrading algae, such as Aquimana, Nonlabens, Alteromonas, Loktanella, also successfully colonized. The yield and quality of N. haitanensis in the 2nd and 3rd harvests are crucial for annual production and income. However, potential pathogenic bacteria and factors peaked in abundance during the 2nd harvest. At this point, the symbiotic body formed by Neoporphyra and the PMC-Nh was in a state of equilibrium on the “tip of the needle”. Biomarkers in the 2nd harvest, such as Nonlabens and Alteromonas, have been reported as algal pathogens. Moreover, pathways or factors associated with bacterial pathogenesis were significantly enriched in the algal-associated microbial community in the 2nd harvest, such as biofilm formation, flagellar assembly, bacterial secretion system pathways, and virulence factors of the Virulence Factors of Pathogenic Bacteria (VFDB) database. This may be attributed to the variable and frequently intense temperature increases in the coastal areas of the East China Sea during the 2nd harvest period in mid to late October, leading to significant shifts in microbial balance. The PMC-Nh remained relatively stable during the 3rd harvest. Until the end of cultivation, pathways related to glycometabolism and the content of glycoside hydrolases in the PMC-Nh increased, possibly due to heightened polysaccharide content and the maturation or senescence of the final stage of N. haitanensis, favoring polysaccharide exudation. Our results suggest that the 2nd harvest stage is a high-risk period for N. haitanensis cultivation, and effective cultivation management should be conducted to prevent widespread algal decay and reduce production losses.

Gender disparities in lost productivity resulting from non-communicable diseases in Mexico, 2005-2021.

Non-communicable diseases (NCDs) cause long-term impacts on health and can substantially affect people's ability to work. Little is known about how such impacts vary by gender, particularly in low- and middle-income countries (LMICs), where productivity losses may affect economic development. This study assessed the long-term productivity loss caused by major NCDs among adult women and men (20-76 years) in Mexico because of premature death and hospitalisations, between 2005 and 2021. We conducted an economic valuation based on the Human Capital Approach. We obtained population-based data from the National Employment Survey from 2005 to 2021 to estimate the expected productivity according to age and gender using a two-part model. We utilised expected productivity based on wage rates to calculate the productivity loss, employing Mexican official mortality registries and hospital discharge microdata for the same period. To assess the variability in our estimations, we performed sensitivity analyses under two different scenarios. Premature mortality by cancers, diabetes, chronic cardiovascular diseases (CVD), chronic respiratory diseases (CRD) and chronic kidney disease (CKD) caused a productivity loss of 102.6 billion international US dollars (Intl. USD) from 2.8 million premature deaths. Seventy-three percent of this productivity loss was observed among men. Cancers caused 38.3% of the productivity loss (mainly among women), diabetes 38.1, CVD 15.1, CRD 3.2, and CKD 5.3%. Regarding hospitalisations, the estimated productivity loss was 729.7 million Intl. USD from 54.2 million days of hospitalisation. Men faced 65.4 and women 34.6% of these costs. Cancers caused 41.3% of the productivity loss mainly by women, followed by diabetes (22.1%), CKD (20.4%), CVD (13.6%) and CRD (2.6%). Major NCDs impose substantial costs from lost productivity in Mexico and these tend to be higher amongst men, while for some diseases the economic burden is higher for women. This should be considered to inform policymakers to design effective gender-sensitive health and social protection interventions to tackle the burden of NCDs.

Leber hereditary optic neuropathy in Czechia and Slovakia: Quality of life and costs from patient perspective

IntroductionLeber hereditary optic neuropathy (LHON) is the most frequent mitochondrial disease causing dyschromatopsia and progressive central visual loss that is subacute in progression and painless. Several studies have been published assessing QoL in patients with LHON, but no estimate of the economic burden has been reported to date. This study aims to quantify direct non-medical and indirect costs (productivity loss) incurred by LHON patients and their informal caregivers in Czechia and Slovakia, as well as to assess their quality of life. MethodsThe study was performed in 27 adults and children with LHON. To determine the socioeconomic burden of LHON, separate questionnaires for adults, children, and their parents were developed, including demographic and socioeconomic data. The following data were collected: age, education, family size, severity of LHON, non-medical direct and indirect costs of LHON. ResultsThe mean age of adult respondents was 36.1 years (SD 13.1; n = 21). The total cost of absenteeism was EUR 1003 per person/year in adult employees, and EUR 2711 per person/year in children's parents. The productivity loss as a consequence of LHON due to combined relative absenteeism and relative presenteeism was estimated at EUR 9840 per an adult patient/year, and EUR 6298 per a parent/year, respectively. The mean cost of informal care was estimated at EUR 4502 (SD 4772; n = 6) per person/year. The mean VFQ-25 score for adult patients with LHON was 43.47 (SD 15.86). ConclusionThe results of this study clearly show that patients with LHON and their families face an extensive socioeconomic burden related to this rare disease. Early, timely and appropriate access to diagnosis, treatment, and reimbursement decisions, but also to psychological counselling and services may help the patients and their relatives adapt and cope with the challenging aspects of vision loss and life with the disease.

Detection of bacterial spot disease on tomato leaves using a Convolutional Neural Network (CNN)

Tomato diseases have become a major concern to the tomato production sector around the world. A huge proportion of tomato crops deteriorate yearly during growth or after harvesting due to the infections caused by fungus, viruses and bacteria. Early detection of these diseases plays a crucial role in alleviating overall production loss. Over the past decades, farmers have been using visual observation to identify a crop disease in a field. However, the visual observation method is labour intensive, time consuming, and prone to human error. Currently, intelligent approaches have been widely used to detect and classify these diseases. The objective of this study is to design a convolutional neural network VGG16 net architecture that is able to detect tomato diseases on tomato leaves. A model that can successfully detect 5 tomato leaf diseases was developed: (1) Bacterial spot, (2) Early blight, (3) Late blight, (4) Septoria leaf and (5) Yellow leaf curl with a training accuracy of 0.9328.

A Chelex-100-based rapid DNA extraction method and its application in the detection of shrimp pathogens

BackgroundThe Pacific white shrimp is one of the world’s most economically significant aquatic species, being one of the top three species cultured globally. However, the increasing incidence of diseases such as acute hepatopancreatic necrosis disease and hepatopancreatic microsporidia has led to a serious decline in shrimp production and severe economic losses. With the increasing demand for pathogen detection in shrimp farms, rapid DNA extraction technology has become more sophisticated. In this study, a rapid and crude method of extracting genomic DNA from shrimp muscle and hepatopancreas using Chelex-100 was established. ResultsDNA was successfully extracted from muscle and hepatopancreatic tissues using both the Chelex-100 method and commercial kits. The internal reference genes of shrimp were successfully amplified via PCR and real-time PCR using the obtained DNA samples. Moreover, a field assay was successfully conducted using real-time PCR and real-time enzymatic recombinase amplification (real-time ERA), indicating that the quality of the DNA extracted using Chelex-100 is sufficient for use in conjunction with nucleic acid amplification to detect pathogens in shrimps. ConclusionsChelex-100 is an efficient method for extracting DNA from shrimp muscle or hepatopancreas tissues, with a short extraction time, high extraction efficiency, and simple operation, making it appropriate for use in the detection of pathogens in shrimp.How to cite: Yang H, Zhou Q, Hu J, et al. A Chelex-100-based rapid DNA extraction method and its application in the detection of shrimp pathogens. Electron J Biotechnol 2024;70. https://doi.org/10.1016/j.ejbt.2024.04.004.

African food system and biodiversity mainly affected by urbanization via dietary shifts

AbstractThe rapid urbanization in Africa profoundly affects local food and ecological systems. According to earlier research, urbanization may cause food production and biodiversity losses as agricultural or natural lands are absorbed by expanding cities. Land-use displacement effects may buffer agricultural production losses or may lead to additional biodiversity losses but are often overlooked. Moreover, impacts of dietary changes associated with urbanization are rarely considered. To address this, we combined spatially explicit projections of African urban area expansion with observed rice consumption shifts to inform a partial equilibrium model (the Global Biosphere Management Model). We demonstrate the importance of displacement effects to identify potential food production or biodiversity issues until 2050 and argue for their integration in land-use planning and policymaking across spatial scales. We identify that because of agricultural displacement, the impact of urban area expansion on food production losses is probably limited (<1%)—at the cost of additional losses of natural lands by 2050 (up to 2 Mt). We also show that considering dietary shifts associated with urbanization increases rice consumption, production (+8.0%), trade (up to +2 Mt of required import) and agricultural methane emissions (up to +12 MtCO2-equivalent yr–1), thereby underscoring the need for a systems approach in future sustainability studies.

Annual Accumulation of CymMV May Lead to Loss in Production of Asymptomatic Vanilla Propagated by Cuttings.

Vanilla (Vanilla planifolia Andrews) is a valuable orchid spice cultivated for its highly priced beans. Vanilla has been planted in Hainan province of China via cutting propagation for about 40 years. The yield has been decreasing annually for the past ten years due to pod numbers declining significantly even though it seems to grow normally without disease symptoms, while the reason is still unknown. In this study, we found that Cymbidium mosaic virus (CymMV), one of the most devastating viruses causing losses in the vanilla industry, massively presented within the pods and leaves of vanilla plants, so the virus infecting the vanilla seems to be a highly probable hypothesis of the main contributions to low yield via decreasing the number of pods. This represents the first speculation of CymMV possibly affecting the yield of vanilla in China, indicating the important role of virus elimination in restoring high yield in vanilla. This research can also serve as a warning to important economic crops that rely on cuttings for propagation, demonstrating that regular virus elimination is very important for these economically propagated crops through cuttings.

Emergence of Lasiodiplodia theobromae induced leaf necrosis in tea (Camellia sinensis [L.] O. Kuntze) from India.

The tea plant, Camellia sinensis [L.] O. Kuntze, is a vital global agricultural commodity, yet faces challenges from fungal infections, which affects its production. To reduce the loss in the tea production, the fungal infections must be removed which is managed with fungicides, which are harmful to the environment. Leaf necrosis, which decreases tea quality and quantity, was investigated across Assam, revealing Lasiodiplodia theobromae as the causative agent. Pathogenicity tests, alongside morphological and molecular analyses, confirmed its role in leaf necrosis. Genome and gene analysis of L. theobromae showed multiple genes related to its pathogenicity. The study also assessed the impact of chemical pesticides on this pathogen. Additionally, the findings in this study highlight the significance of re-assessing management approaches in considering the fungal infection in tea.

Complex Forest Tree Diseases – Diagnostics Beyond Koch’s Postulates

Abstract Purpose of Review Forest tree diseases are a major contributor to forest degradation and loss of productivity. They are often quite complex in their causation (etiology), especially in the case of forest syndromes, i.e. diseases with multiple causes and concurrent symptoms. Traditionally, to prove pathogenicity of a microbial agent, and thus correctly diagnose the etiology of a disease, plant pathologists must satisfy all of the so-called Koch’s postulates, as mandated by their deontological code. This review examines whether this approach is still current. Recent Findings Koch’s postulates state that a pathogen is a microorganism that, after being isolated in pure culture, can reproduce the disease when it is inoculated into a healthy plant. Over the decades, plant pathologists as well as medical scientists have discovered that these postulates are not always applicable in their entirety and that, furthermore, novel approaches based on molecular biology can be very helpful in uncovering relationships between microbes and diseases that are not easily proven using Koch’s postulates. Summary I conclude that Koch’s postulates are not a viable approach for many forest tree diseases and propose a set of new guidelines, based on the preponderance of the evidence principle, to integrate this proven approach and bring it into the twenty-first century.

The Burden of Work Productivity and Activity Impairment in Patients with Psoriasis, Psoriatic Arthritis, Ulcerative Colitis, and Crohn's Disease.

Comparisons among autoimmune diseases enable understanding of the burden and factors associated with work productivity loss and impairment. The objective was to compare work productivity and activity and associated factors among patients with inflammatory bowel diseases and other autoimmune conditions. This cross-sectional study included employed, adult patients (age 20-64 years) in the CorEvitas Inflammatory Bowel Disease, Psoriasis, and Psoriatic Arthritis/Spondyloarthritis Registries between 5/2017 and 6/2020. Any patient-reported impairment on four domains of the Work Productivity and Activity Index (WPAI) was collected across registries. Prevalence for each autoimmune disease was reported and stratified by disease activity using direct age-sex-standardization. Factors associated with the presence of any WPAI were identified in logistic regression models. A total of 7,169 patients with psoriasis (n = 4,768, 67%), psoriatic arthritis (n = 1,208, 17%), Crohn's disease (CD, n = 621, 9%), and ulcerative colitis (UC, n = 572, 8%) met inclusion criteria. Among patients not in remission across all disease cohorts, the age-sex-standardized prevalence of any presenteeism, work productivity loss, and activity impairment ranged from 54 to 97%. Patients with CD in remission had higher standardized prevalence of presenteeism (53% [48-57%]) and work productivity loss (54% [49-59%]), compared to those from other cohorts (presenteeism [range: 33-39%] and work productivity loss [range: 37-41%]). For all WPAI domains, the strongest adjusted associations were for moderate to severe disease activity and psychosocial symptoms. Patients with moderate to severe disease activity reported the highest WPAI burden. However, patients in remission or mild disease activity also report some WPAI burden, emphasizing a multidisciplinary treatment approach to improve work productivity loss and impairment.

PDHL-MARCOS method based on cumulative prospect theory for MAGDM and its application in network security evaluation

The increasingly large and complex network brings convenience to our life and production, but it also brings potential dangers. Any minor vulnerability may lead to failure of application software configuration, software maintenance, user management and system management. Eventually, our network is interrupted, and even life and production data are leaked. Therefore, establishing a scientific and effective network security evaluation model is an effective way to supervise, manage and improve network security; It is also an effective measure to reduce the loss of life and production. Therefore, we first selected six factors closely related to network security to form a complete network security evaluation index system. Then, the utility function in the traditional measurement of alternatives and ranking according to compromise solution (MARCOS) method is improved by using the value function in cumulative prospect theory (CPT), and a PDHL-MARCOS evaluation model that can reflect the decision-makers’ (DMs’) attitudes towards gains and losses is obtained in the probabilistic double hierarchy linguistic term set (PDHLTS). In addition, the relative entropy (RE) method is also used to obtain expert weights. The combination weights of attributes are obtained using the correlation coefficient and the standard deviation (CCSD) method and the weight function in CPT.

Modelling error chains in offshore wind energy systems: Examining the interplay of human performance and machine state

A dynamic model of the mutual interdependency between humans and machines in offshore wind farms (OWFs) is developed in this paper. The model emphasises the importance of having early indicators to dynamically moderate human behaviour and the need to account for both human and physical subsystems and their mutual interactions for optimal asset management practices. This work simulates three distinct scenarios for: (1) examining the interconnectedness of technical and human dynamics and their implications on error and failure; (2) assessing the impact of production loss on human and organisational behaviour; and (3) evaluating human error probability as an early warning sign of production loss. The findings suggest that adopting an appropriate maintenance culture and considering human error likelihood and production rate in decision-making leads to optimising production and mitigating the risks associated with human error. The research highlights the significance of comprehending the complex interactions between human and machine factors in the operation and maintenance (O&M) of offshore wind turbines (OWTs). The proposed dynamic model helps organisations identify the underlying causes of errors, allowing them to improve their maintenance strategies.

Removal of empty capsids from high-dose adeno-associated virus 9 gene therapies.

Recombinant adeno-associated virus, serotype 9 (rAAV9) has shown promise as a gene therapy vector for muscle and central nervous diseases. High-dose requirements of these therapies present critical safety considerations and biomanufacturing challenges. Notably, the reduction of empty capsids (ECs), which lack therapeutic transgene, from rAAV9 products is critical to maximize efficacy. Removal of rAAV ECs from full capsids is a major downstream challenge because of their highly similar biophysical characteristics. Ultracentrifugation (UC) reduces ECs but is laborious and difficult to scale. In this paper, to replace a poorly scalable UC process, we developed an anion exchange (AEX) chromatography for rAAV9 EC reduction from full capsids. AEX load preparation by dilution incurred major product loss. The addition of histidine and surfactants to dilution buffers increased yield and reduced aggregation. Elution salts were screened and sodium acetate was found to maximize yield and EC reduction. The most promising load dilution buffer and elution salt were used in combination to form an optimized AEX method. The process reduced ECs three-fold, demonstrated robustness to a broad range of EC load challenges, and was scaled for large-scale manufacture. Compared to UC, the AEX method simplified scale-up, reduced ECs to comparable levels (20%), afforded similar purity and product quality, and increased yield by 14%.

Direct and indirect costs of paediatric asthma in the UK: a cost analysis

ObjectiveTo estimate the cost of paediatric asthma from a UK National Health Service (NHS) and societal perspective and explore determinants of these costs.DesignCost analysis based on data from a large...

Optimal design of hydrogen production processing coupling alkaline and proton exchange membrane electrolyzers

This study considered complementation of the different electrolyzers and designed a new hydrogen production system coupling alkaline (ALK) electrolyzers and the proton exchange membrane (PEM) electrolyzers. A collaborative optimization method is proposed to design both the capacities and the annual energy scheduling of the system. Due to the different operation characteristics and parameters of the two electrolyzers, the operation states of them are described separately in the model. Besides, several practical factors are also considered, including electrolyzer grouping, hydrogen production loss, adjustment response efficiency and switch-on times. According to the calculation results, the annual income of the coupling system is 6.0 % and 28.9 % higher than that of the ALK only and PEM only system. The installed load rates of PEM electrolyzers to the coupling systems are between 10 and 20 %. Furthermore, the influences of multiple factors on the coupling system are also analyzed. After analysis and comparison, hydrogen production and system economy rise with increased wind power installed proportion is rising. Besides, the increase of the allowed proportions of electricity purchase can enhance the stability of system power supply, and improve the hydrogen production and economy.

Resilient Response to Combined Heat and Drought Stress Conditions of a Tomato Germplasm Collection, Including Natural and Ethyl Methanesulfonate-Induced Variants

Agricultural systems are currently facing significant issues, primarily due to population growth rates in the context of global climate change. Rising temperatures cause plant heat stress and impact crop yield, which in turn compromises global food production and safety. Climate change is also having a significant impact on water availability around the world, and droughts are becoming more frequent and severe in many regions. The combined effect of both heat and drought stresses increases plant damage, resulting in reduced plant development and productivity loss. Therefore, developing heat–drought-tolerant crop varieties is crucial for enhancing yield under these challenging conditions. Tomato (Solanum lycopersicum L.), a major vegetable crop highly appreciated for its nutritional qualities, is particularly sensitive to extreme temperatures, which have a significant negative impact on tomato fruit setting and cause male gametophyte abortion. In this work, a classical genetic approach was employed to identify tomato genotypes showing a resilient response to combined heat and drought stress conditions. A phenotype screening of a natural germplasm collection and an ethyl methanesulfonate (EMS) mutagenized population resulted in the identification of a significant number of tomato lines tolerant to combined heat and drought conditions, specifically 161 EMS lines and 24 natural accessions as tolerant. In addition, TILLING and Eco-TILLING analyses were used as proof-of-concept to isolate new genetic variants of genes previously reported as key regulators of abiotic stress responses in different species. The identification of these variants holds the potential to provide suitable plant material for breeding programs focused on enhancing tomato resilience to adverse climate conditions.

Instrumental and sensory tools to evaluate the production potential of a new type of sparkling wine from umbu (Spondias tuberosa)

Umbu is a native fruit from the Brazilian Caatinga Biome, which is a source of income for families involved in extractivism. This fruit is highly perishable and production losses have been experienced. The production of sparkling wine can be a strategy for better use and to add value to this fruit. Furthermore, consumers have been attracted to innovative beverages, produced with alternative raw materials and with an exotic and pleasant aroma. The potential for producing sparkling wine from umbu was evaluated through physical-chemical, instrumental (gas chromatography with detection by mass spectrometry and olfactometry) and sensory analyses (acceptance test, Just-about-right (JAR) scale, and penalty analysis). Both the base wine and the sparkling wine meet all regulatory physicochemical parameters. The volatile profile of umbu pulp contains mainly terpenes (55%), while esters predominated in the base and sparkling wines (32 and 33%, respectively). In sparkling wines, hexanoic acid (3114 mg L−1) and linalool (1266 mg L−1) were the major compounds. Among the 26 odor-active compounds of sparkling wines are mainly ethyl esters with fruity aroma (acetate, decanoate, and 2-phenylacetate; OSME area: 4.8, 4.8, and 5.0%, respectively), and terpenes with floral notes (p-cymene, geranic oxide and linalool; OSME area: 8.5, 7.4, and 6.9%, respectively). The acceptance and purchase intention tests endorsed the commercialization expectation, although JAR scale and penalty analysis showed that the umbu aroma and sweetness may be improved. The potential of umbu for the production of sparkling wines has been proven, which satisfies the current market demand for innovative beverages.

Farmers’ Adoption, Knowledge, and Perceptions of Tick Control Measures on Dairy Farms in Subtropical Areas of Continental Ecuador

The application of tick control strategies on tropical dairy cattle strongly relies on farmers’ uptake, knowledge, and perceptions of the efficacy of control measures. This study aims to identify common and uncommon tick control practices employed by dairy farmers in subtropical areas of Ecuador and associate them with the presence of infestation and acaricide resistance. Data were collected through a cross-sectional survey and participatory meetings. Multiple correspondence analysis was used to explore the association between management variables and the level of tick infestation and resistance. It was determined that the main method of acaricide control is still chemical, mainly using spray baths. Generally, when this form of application is used, acaricides are overdosed, in contrast to the pour-on method with underdosage. Among the measures farmers adopt when chemical treatment has failed is to use overdoses of products, mix different acaricides, and use focused treatments (wipe cloth) with irritant substances. The absence of a high level of infestation was related to acaricide dips every 3–4 weeks and the use of intensive grazing. On the other hand, the high infestation was related to the use of organophosphates, wipe cloth application, and the report of tick-borne diseases (TBDs). A small group of farmers have good knowledge and seek alternatives to chemical control, experimenting with biological controls, herbal extracts, manual tick removal, and paddock control. Additionally, farmers reported the presence of TBDs (47%) and the presence of animals poisoned by acaricides (6%), which died in 75% of those cases. Farmers frequently mentioned that tick infestation induces milk drop production and weight loss and is associated with the presence of TBDs. This information is crucial to improve tick control management in Ecuador, particularly through implementing practices that mitigate resistance to acaricides and ensure long-term solutions that help maintain the efficacy of tick control treatments.

Cross-Inoculation of Commercial Forestry, Amenity, and Horticulture Tree Species with Ceratocystis Isolates Collected from Different Host Species.

Ceratocystis manginecans has caused significant losses in forestry productivity in Indonesia and neighboring nations. It also infects horticultural trees, but the host range of individual isolates of C. manginecans is poorly studied. So, this study aimed to better understand the potential host range and evaluate aggressiveness against forestry and fruit tree species of C. manginecans isolated from various tree species in Indonesia. Five C. manginecans isolates, four from different tree species and one from the shot-hole borer Euwallacea perbrevis, were used to inoculate seven fruit and six forest tree species, including E. pellita and Acacia mangium. Many of the inoculated trees produced typical canker disease symptoms, such as rough, swollen, and cracked lesions on the bark, but some trees did not have any external symptoms. Mortality in the most susceptible clone of A. mangium was 40% within 8 weeks. Forest tree species were more susceptible than fruit trees, with the length of xylem discoloration ranging from 0.4 to 101 cm. In fruit trees, the average extent of xylem discoloration was lower, ranging from 0.4 to 20.5 cm; however, mortalities were recorded in two fruit tree species, Citrus microcarpa and Durio zibethinus. Host-isolate interaction was evident; isolate Ep106C from Eucalyptus pellita caused the greatest xylem discoloration in Citrus sp., whereas Hy163C from Hymenaea courbaril was the most damaging in D. zibethinus, Artocarpus heterophyllus, and Mangifera indica. Increasingly globalized food and fiber systems increase risk of disease spread, and the serious threat of C. manginecans incursions into countries where it is not present must be evaluated more thoroughly.

Fluorescently Tagged Verticillium dahliae to Understand the Infection Process on Cotton (Gossypium hirsutum) and Weed Plant Species.

Verticillium wilt is a soil-borne disease caused by distinct vegetative compatibility groups (VCG) of the fungus Verticillium dahliae. Defoliating (VCG 1A) and non-defoliating (VCG 2A) pathotypes of V. dahliae have contributed to yield losses of cotton production in Australia. To study the virulence and the infection process of V. dahliae on cotton, two isolates, one representing each VCG, have been transformed with fluorescent protein genes. The transformants maintained their ability to infect the host, and both strains were observed to move through the plant vasculature to induce wilt symptoms. Furthermore, virulence testing suggests that the cotton V. dahliae strains can endophytically colonise common weed plant species found in the Australian landscape, and that is contrasted by their ability to infect and colonise native tobacco plants. The fluorescently labelled strains of V. dahliae not only allowed us to gain a thorough understanding of the infection process but also provided a method to rapidly identify recovered isolates from host colonisation studies.