Longer Segments Research Articles

Introduction to the Third Generation Simulation Dataset: Data Collection and Trajectory Extraction

This study aims to provide accurate trajectory datasets capable of characterizing human–automated vehicle interactions under a diverse set of scenarios in diverse highway environments. Distinct methods were utilized to collect data from Level 1, Level 2, and Level 3 automated vehicles: (1) fixed location aerial videography (a helicopter hovers over a segment of interest); (2) moving aerial videography (a helicopter follows the automated vehicles as they move in a much longer segment than in the first method); and (3) infrastructure-based videography (multiple overlapping cameras located on overpasses creating a comprehensive image of the study area). Utilizing the fixed location aerial videography approach, trajectories were extracted on I-90/I-94 in Chicago, IL. The moving aerial videography approach was adopted to extract four datasets on I-90/I-94 and I-294 in Chicago, IL. Finally, two datasets were collected on I-395 and George Washington University Campus in Washington, D.C., using the infrastructure-based videography approach. Extracting multiple complete and accurate vehicle trajectories raises a set of methodological and practical challenges that vary across the three data measurement approaches. The methodological details to extract these trajectories are presented in this paper along with the lessons learned with respect to data collection setup, instrumentation, and experimental design efforts.

LST-EMG-Net: Long short-term transformer feature fusion network for sEMG gesture recognition.

With the development of signal analysis technology and artificial intelligence, surface electromyography (sEMG) signal gesture recognition is widely used in rehabilitation therapy, human-computer interaction, and other fields. Deep learning has gradually become the mainstream technology for gesture recognition. It is necessary to consider the characteristics of the surface EMG signal when constructing the deep learning model. The surface electromyography signal is an information carrier that can reflect neuromuscular activity. Under the same circumstances, a longer signal segment contains more information about muscle activity, and a shorter segment contains less information about muscle activity. Thus, signals with longer segments are suitable for recognizing gestures that mobilize complex muscle activity, and signals with shorter segments are suitable for recognizing gestures that mobilize simple muscle activity. However, current deep learning models usually extract features from single-length signal segments. This can easily cause a mismatch between the amount of information in the features and the information needed to recognize gestures, which is not conducive to improving the accuracy and stability of recognition. Therefore, in this article, we develop a long short-term transformer feature fusion network (referred to as LST-EMG-Net) that considers the differences in the timing lengths of EMG segments required for the recognition of different gestures. LST-EMG-Net imports multichannel sEMG datasets into a long short-term encoder. The encoder extracts the sEMG signals' long short-term features. Finally, we successfully fuse the features using a feature cross-attention module and output the gesture category. We evaluated LST-EMG-Net on multiple datasets based on sparse channels and high density. It reached 81.47, 88.24, and 98.95% accuracy on Ninapro DB2E2, DB5E3 partial gesture, and CapgMyo DB-c, respectively. Following the experiment, we demonstrated that LST-EMG-Net could increase the accuracy and stability of various gesture identification and recognition tasks better than existing networks.

Genetic characterization of Mozambican Nguni cattle and their relationship with indigenous populations of South Africa

Knowledge of genetic variability among cattle populations is essential to gain insight into the adaptation mechanisms to different environments and to support the conservation of genetic resources. Individuals from Mozambican Nguni (MZ Nguni; n = 119), South African Nguni (SA Nguni; n = 150), South African Tuli (SA Tuli; n = 150), and South African Boran (SA Boran; n = 150) cattle populations were genotyped using the International Dairy and Beef SNP BeadChip version three (IDB) and the GeneSeek Genomic Profiler (GGP 80k) assays, to investigate their levels of genetic diversity and the relationships between these indigenous breeds. Levels of genetic diversity, assessed by expected heterozygosity (He), varied from 0.284 (SA Boran) to 0.324 (SA Tuli). Population structure, as well as principal component analysis (PCA), revealed tight clustering of the two Nguni populations, while the SA Tuli and SA Boran populations diverged, as expected, into two distinct clusters. Little genetic distance (0.031) was observed among MZ Nguni and SA Nguni, while SA Boran (a Zebu breed) was further removed from SA Tuli than from the other Sanga cattle populations. Runs of homozygosity (ROH) analysis revealed low inbreeding rates (with the average FROH per population ranging from 0.003 to 0.006). Short ROH segments (ROH ≤ 5 Mb) were more frequent in all four populations than longer segments, suggesting more ancient inbreeding in these populations. The highest number of ROH (303) was observed in SA Tuli, while the lowest (56) was detected in SA Nguni. Analysis of both Wright's fixation index (FST) and ratio of extended haplotype homozygosity (Rsb) identified a total of 229 differentiated single nucleotide polymorphisms (SNP) to be under selection, in a comparison between the MZ Nguni cattle and South African cattle populations. Highly differentiated SNP (FST ≥ 0.26 or pRsb ≥ 3) indicated genes including KLHL29, ZEB2, LAMC1, MYLK3, and KCNK5 that are implicated in several metabolic processes essential for adaptation and production traits.

Potentialities and limitations of machine learning to solve cut-and-shuffle mixing problems: A case study

Cut-and-shuffle mixing is an instructive candidate system with which to assess the potential of machine learning (ML) as an approach to solve difficult mixing problems. We focus on a specific subset of cut-and-shuffle systems, the one-dimensional interval exchange transform. This class of mixing operations is well studied, and a simple mixing methodology, which we refer to as the longest segment (LS) method, works well under a broad range of situations. We use supervised learning to train a neural network (NN) to emulate the LS mixing algorithm for mixing a one-dimensional domain of two species. We find that a generic deep NN can emulate the LS method with good accuracy but cannot generalize to conditions significantly outside its training repertoire. The challenges in defining the mixing problem and generalizing a ML mixing approach are indicative of those expected for more complex systems where optimal or near optimal mixing methods remain unknown.

Restoration of vertebral body height in traumatic thoracolumbar fractures: open versus minimal invasive surgery – which is better?

Background Percutaneous minimally invasive surgery (MIS) has been increasingly implemented to treat thoracolumbar (TL) fracture against a conventional open pedicle screw fixation (OPSF) with desirable radiological and clinical outcomes. Studies in the Indian context are required to determine the efficiency of MIS over OPSF. Objective To compare restoration and maintenance of vertebral body height (VBH) following MIS versus OPSF. Methods A prospective comparative study was conducted in a tertiary care hospital. Patients (n= 36) aged 18–65 years (males = 23, females = 13) with traumatic TL fractures were identified. Eighteen of them underwent OPSF and other eighteen underwent MIS. The radiological outcomes like anterior and posterior vertebral body height percentage (AVBH % and PVBH %) restoration and maintenance were evaluated. Quantitative variables were analyzed and described using mean ± standard deviation and qualitative variables were presented using frequency and percentage. Student t-test was used to analyse continuous data and Chi-square/Fisher Exact test was used to analyse categorical data. Results The mean age of the patients was 38.8 years. The majority of fractures were seen in the T12-LI segment (52.7 %). The AVBH % restoration and maintenance in OPSF was significantly higher compared to that of MIS at immediate post-operative (IPO) period (p = 0.01), 6 weeks (p = 0.02) and 12 weeks (p = 0.006) post-surgery. Long segment stabilization provided statistically significant AVBH and PVBH values for OPSF compared to MIS (p < 0.05). The presence or absence of pedicle screw at fractured vertebral level did not restore AVBH and PVBH to a statistically significant level in both the groups (p > 0.05). Superficial surgical site infection (SSSI) was seen in both the groups without any statistical significance between them (p > 0.05). Conclusion OPSF is superior over MIS in the restoration of AVBH. Restoration of PVBH was also better with OPSF although not statistically significant. OPSF with longer segment fixation had better restoration of both AVBH and PVBH. The presence or absence of pedicle screw at fractured vertebral level did not seem to have any significance in both the groups. However, the MIS approach can be a reasonable alternative to open surgery with potential advantages like better clinical and functional outcome. A selected population of patients treated with MIS will show better surgical outcomes.

ELSED: Enhanced line SEgment drawing

Detecting local features, such as corners, segments or blobs, is the first step in the pipeline of many Computer Vision applications. Its speed is crucial for real-time applications. In this paper we present ELSED, the fastest line segment detector in the literature. The key for its efficiency is a local segment growing algorithm that connects gradient-aligned pixels in presence of small discontinuities. The proposed algorithm not only runs in devices with very low end hardware, but may also be parametrized to foster the detection of short or longer segments, depending on the task at hand. We also introduce new metrics to evaluate the accuracy and repeatability of segment detectors. In our experiments with different public benchmarks we prove that our method accounts the highest repeatability and it is the most efficient in the literature.11Source code: https://github.com/iago-suarez/ELSED In the experiments we quantify the accuracy traded for such gain.

Leftward Spatial Bias in Dialyzed Patients With End-Stage Renal Disease: A Sign of Right Hemispheric Attentional Activation or Impaired Left-Sided Disengagement?

In the horizontal line bisection task, patients with end-stage renal disease (ESRD) treated with dialysis typically have an increase in the normal leftward attentional bias. It is not clear, however, whether this increased bias is related to an increase of attention toward left hemispace or impaired disengagement from left-sided stimuli. Non-demented patients with ESRD who were being treated with dialysis and demographically matched healthy controls attempted to bisect series of horizontal lines composed of two segments of unequal length and width, with the longer segment placed to the right or left. To test for the presence of a global spatial attentional bias, participants attempted to find the middle of the whole line. To test for focal attentional engagement and attentional disengagement, participants were asked to bisect only the longer segment of these compound lines. For the global condition, both patients and healthy controls were biased toward the longer segment and more so to the left. In the focal condition, when the longer segment was on the right side, both groups deviated their bisections toward the shorter segment. However, when in the focal condition the longer segment was on the left healthy controls deviated their bisection significantly more rightwards than did the patients, whereas with the longer segment on the right, the patients and healthy controls did not differ in their bias. These results suggest that the previously recognized dysfunction of the right fronto-subcortical attentional network in dialyzed patients might account for the results of the current study.

Correspondence Between Genomic- and Genealogical/Coalescent-Based Inference of Homozygosity by Descent in Large French-Canadian Genealogies.

Research on the genetics of complex traits overwhelmingly focuses on the additive effects of genes. Yet, animal studies have shown that non-additive effects, in particular homozygosity effects, can shape complex traits. Recent investigations in human studies found some significant homozygosity effects. However, most human populations display restricted ranges of homozygosity by descent (HBD), making the identification of homozygosity effects challenging. Founder populations give rise to higher HBD levels. When deep genealogical data are available in a founder population, it is possible to gain information on the time to the most recent common ancestor (MRCA) from whom a chromosomal segment has been transmitted to both parents of an individual and in turn to that individual. This information on the time to MRCA can be combined with the time to MRCA inferred from coalescent models of gene genealogies. HBD can also be estimated from genomic data. The extent to which the genomic HBD measures correspond to the genealogical/coalescent measures has not been documented in founder populations with extensive genealogical data. In this study, we used simulations to relate genomic and genealogical/coalescent HBD measures. We based our simulations on genealogical data from two ongoing studies from the French-Canadian founder population displaying different levels of inbreeding. We simulated single-nucleotide polymorphisms (SNPs) in a 1-Mb genomic segment from a coalescent model in conjunction with the observed genealogical data. We compared genealogical/coalescent HBD to two genomic methods of HBD estimation based on hidden Markov models (HMMs). We found that genomic estimates of HBD correlated well with genealogical/coalescent HBD measures in both study genealogies. We described generation time to coalescence in terms of genomic HBD estimates and found a large variability in generation time captured by genomic HBD when considering each SNP. However, SNPs in longer segments were more likely to capture recent time to coalescence, as expected. Our study suggests that estimating the coalescent gene genealogy from the genomic data to use in conjunction with observed genealogical data could provide valuable information on HBD.

A retrospective anatomical study of the cerebral dural venous sinus outflow pathways utilizing three-dimensional rotational venography

OBJECTIVE:Proper blood flow is essential for the maintenance of homeostasis for the human cerebrum. The dural venous sinuses comprise the dominant cerebral venous outflow path. Understanding the spatial configuration of the dural venous sinuses can provide valuable insight into several pathological conditions. Previously, only two-dimensional or cadaveric data have been used to understand cerebral outflow. For the first time, we applied three-dimensional rotational venography (3D-RV) to study and provide detailed quantitative morphological measurements of the terminal cerebral venous sinus system in several pathological states.SUBJECTS AND METHODS:Patients who underwent a 3D-RV procedure were identified by reviewing our local institution's endovascular database. Patients with high-quality angiographic images were selected. Eighteen patients were included (37.1 ± 3.8 years). Sinuses were divided into four segments, starting at the torcula and ending at the internal jugular vein. Segment length, 3D displacement, and cross-sectional area were measured.RESULTS:The transverse sinus (60.2 mm) was the longest segment, followed by the sigmoid sinus (55.1 mm). Cross-sectional areas were smallest at the middle of the transverse sinus (21.3 mm2) but increased at the sigmoid sinus (33.5 mm2) and at the jugular bulb (49.7 mm2). The only variation in displacements of venous flow was at the sigmoid-jugular junction, where 55% of cases had lateral displacements versus 45% medial, and 78% superior versus 22% inferior.CONCLUSIONS:We describe the terminal venous sinus system of patients with a variety of diagnoses, detailing segment length, cross-sectional area, and 3D path.

Co-existing inflammatory bowel disease and Barrett's esophagus is associated with esophageal dysplasia: a propensity score-matched cohort.

Background and study aims Barrettʼs esophagus (BE) and inflammatory bowel disease (IBD) predispose to the development of dysplasia and cancer. It is unclear if the inflammatory cascade seen in IBD affects disease progression in BE. We aimed to determine if patients with BE who have co-existing IBD had a higher risk of dysplasia, nodular disease, or longer segments than BE patients without IBD. Patients and methods This was a multicenter, retrospective propensity score-matched cohort study. We compared rates of dysplasia, nodular disease, and segment length in patients with BE and IBD (cases) to patients with BE who did not have IBD (controls). Controls were 1:1 propensity score matched with controls for age, sex, body mass index (BMI), smoking, and hiatal hernia. Results A total of 132 patients were included in the IBD + BE group and 132 patients in the BE group. Patients with IBD + BE had higher rates of esophageal dysplasia compared to controls (15.9 % vs. 6.1 % [adjusted odds ratio [OR]: 2.9, 95 % CI: 1.2–6.9]) and more nodules (9.8 % vs. 3.0 % [adjusted OR: 3.5, 95 % CI: 1.1–11.0]). IBD + BE group was also associated with longer BE segments (43.9 % vs. 12.1 % [OR: 5.7, 95 % CI: 3.0–10.6]). Conclusions Co-existing IBD may increase the risk of dysplasia and esophageal nodules in patients with BE. Our findings may have implications for BE surveillance intervals in IBD patients. Prospective studies are needed to confirm our findings.

Automated Detection of Arm-Level Alterations for Individual Cancer Patients in the Clinical Setting

Copy number alterations are genetic events that promote tumor initiation and progression and are used in clinical care as diagnostic, prognostic, and predictive biomarkers. Based on the length of the alteration, they are roughly classified as focal and arm-level alterations. Although genome-wide techniques to detect arm-level alterations are gaining momentum in hospital laboratories, the high precision and novelty of these techniques pose new challenges: there is no consensus on the definition of an arm-level alteration and there is a lack of tools to compute them for individual patients. Based on 376 clinical samples analyzed with the OncoScan formalin-fixed, paraffin-embedded assay, a bimodal distribution of the percentage of bases with copy number alterations within a chromosomal arm was observed, with the second peak starting at 90% of arm length. Two approaches were tested for the definition of arm-level alterations: sum of altered segments (SoS) >90%, or the longest segment (LS) >90%. These approaches were validated against expert annotation of 25 clinical cases. The SoS method outperformed the LS method as indicated by a higher concordance (SoS, 95.2%; LS, 79.9%). Some of the discordances ultimately were attributed to human error, highlighting the advantages of automation. The increase in reliability led to the development of publicly available software and its inclusion into routine clinical practice at Geneva University Hospitals.

Racial Disparities in Adherence to Quality Indicators in Barrett's Esophagus: An Analysis Using the GIQuIC National Benchmarking Registry.

Racial disparities in outcomes in esophageal adenocarcinoma are well established. Using a nationwide registry, we aimed to compare clinical and endoscopic characteristics of blacks and whites with Barrett's esophagus (BE) and adherence to defined quality indicators. We analyzed data from the Gastrointestinal Quality Improvement Consortium Registry between January 2012 and December 2019. Patients who underwent esophagogastroduodenoscopy with an indication of BE screening or surveillance, or an endoscopic finding of BE, were included. Adherence to recommended endoscopic surveillance intervals of 3-5 years for nondysplastic BE and adherence to Seattle biopsy protocol were assessed. Multivariate logistic regression was conducted to assess variables associated with adherence. A total of 100,848 esophagogastroduodenoscopies in 84,789 patients met inclusion criteria (blacks-3,957 and whites-96,891). Blacks were less likely to have histologically confirmed BE (34.3% vs 51.7%, P < 0.01), had shorter BE lengths (1.61 vs 2.35 cm, P < 0.01), and were less likely to have any dysplasia (4.3% vs 7.1%, P < 0.01). Although whites were predominantly male (62.2%), about half of blacks with BE were female (53.0%). Blacks with nondysplastic BE were less likely to be recommended appropriate surveillance intervals (OR 0.78; 95% CI 0.68-0.89). Adherence rates to the Seattle protocol were modestly higher among blacks overall (OR 1.12, 95% CI 1.04-1.20), although significantly lower among blacks with BE segments >6 cm. The use of sex as a risk factor for BE screening may be inappropriate among blacks. Fewer blacks were recommended appropriate surveillance intervals, and blacks with longer segment BE were less likely to undergo Seattle biopsy protocol.

Correlation between great saphenous length of treatment zone and diameter with improvement in symptoms after ablation

ObjectiveThe aim of the present study was to examine the relationship between the great saphenous vein (GSV) length of segment ablated and diameter with symptom improvement. MethodsData from a multicenter, randomized, controlled prospective study of 242 patients undergoing radiofrequency ablation (RFA) or cyanoacrylate closure (CAC) of the GSV were analyzed. The venous clinical severity score (VCSS) was measured at baseline and at 1, 3, 6, 12, 24, and 36 months after ablation. The GSV diameter was evaluated for a correlation with VCSS at each time point. Using the median treatment length of 34 cm, the patients were divided into group I (treatment length ≥34 cm) vs group II (<34 cm) for analysis. The pretreatment VCSS and VCSS improvement (ΔVCSS) after treatment were evaluated with respect to the length of the ablated GSV segment. The postablation VCSS was compared between the pretreatment GSV diameters of <5.5 mm vs ≥5.5 mm. ResultsThe mean GSV length ablated by RFA was 35.3 ± 14 cm vs 32.6 ± 11 cm with CAC (P = NS). No significant difference was found in the VCSS (at baseline or follow-up) between RFA and CAC. The pooled data for all participants (n = 242) demonstrated a correlation between the treated GSV length and pretreatment VCSS and the ΔVCSS at 36 months (R = 0.23; P < .001). Greater VCSS improvement was seen in group I (≥34 cm ablated) than in group II (<34 cm ablated) at ≤36 months of follow-up (P = .003). At baseline, group I had had higher VCSSs (6.21 ± 2.75 vs 4.88 ± 2.33; P < .00001) and CEAP (clinical, etiologic, anatomic, pathophysiologic) and reflux scores. Of the 242 patients, 101 had had a GSV diameter <5.5 mm and 141 had had a GSV diameter of ≥5.5 mm. No significant differences were noted in the VCSSs, either at baseline or at follow-up, between the two groups. Within the study population, a poor correlation was found between the GSV diameter and baseline VCSS (R = −0.004; P = .95) and between the GSV diameter and ΔVCSS for ≤36 months (R = 0.04; P = .55). ConclusionsLonger segments of GSV reflux appeared to correlate with symptom severity. We found a small to moderate correlation between the length of GSV segment ablated and symptom improvement. This might reflect the greater severity of symptoms at baseline in patients with longer segments with reflux. We found a poor correlation between the proximal GSV diameter and symptom improvement after ablation.

Genetic Background and Inbreeding Depression in Romosinuano Cattle Breed in Mexico.

Simple SummaryThe objective of this study was to evaluate the genetic background and inbreeding depression in the Mexican Romosinuano cattle using pedigree and genomic information. Inbreeding was estimated using pedigree () and genomic information based on the genomic relationship matrix () and runs of homozygosity (). Linkage disequilibrium () was evaluated using the correlation between pairs of loci, and the effective population size () was calculated based on and pedigree information. The pedigree file consisted of 4875 animals; 71 had genotypes. decreased with the increase in distance between markers, and estimated using genomic information decreased from 610 to 72 animals (from 109 to 1 generation ago), the estimated using pedigree information was 86.44. The number of runs of homozygosity per animal ranged between 18 and 102 segments with an average of 55. The average inbreeding was 2.98 ± 2.81, 2.98 ± 4.01, and 7.28 ± 3.68% for , , and , respectively. A 1% increase in inbreeding decreased birth weight by 0.103 kg and weaning weight by 0.685 kg. A strategy such as optimum genetic contributions to maximize selection response and manage the long-term genetic variability and inbreeding could lead to sustainable breeding programs for the Mexican Romosinuano cattle breed.The ultimate goal of genetic selection is to improve genetic progress by increasing favorable alleles in the population. However, with selection, homozygosity, and potentially harmful recessive alleles can accumulate, deteriorating genetic variability and hampering continued genetic progress. Such potential adverse side effects of selection are of particular interest in populations with a small effective population size like the Romosinuano beef cattle in Mexico. The objective of this study was to evaluate the genetic background and inbreeding depression in Mexican Romosinuano cattle using pedigree and genomic information. Inbreeding was estimated using pedigree () and genomic information based on the genomic relationship matrix () and runs of homozygosity () of different length classes. Linkage disequilibrium () was evaluated using the correlation between pairs of loci, and the effective population size () was calculated based on and pedigree information. The pedigree file consisted of 4875 animals born between 1950 and 2019, of which 71 had genotypes. decreased with the increase in distance between markers, and estimated using genomic information decreased from 610 to 72 animals (from 109 to 1 generation ago), the estimated using pedigree information was 86.44. The reduction in effective population size implies the existence of genetic bottlenecks and the decline of genetic diversity due to the intensive use of few individuals as parents of the next generations. The number of runs of homozygosity per animal ranged between 18 and 102 segments with an average of 55. The shortest and longest segments were 1.0 and 36.0 Mb long, respectively, reflecting ancient and recent inbreeding. The average inbreeding was 2.98 ± 2.81, 2.98 ± 4.01, and 7.28 ± 3.68% for , , and , respectively. The correlation between and was −0.25, and the correlations among and of different length classes were low (from 0.16 to 0.31). The correlations between and of different length classes were moderate (from 0.44 to 0.58), indicating better agreement. A 1% increase in population inbreeding decreased birth weight by 0.103 kg and weaning weight by 0.685 kg. A strategy such as optimum genetic contributions to maximize selection response and manage the long-term genetic variability and inbreeding could lead to more sustainable breeding programs for the Mexican Romosinuano beef cattle breed.

Numerical investigation of third-order resonant interactions between two gravity wave trains in deep water

Intense resonant interactions between two deep-water gravity waves can lead to the bending and then splitting of crests and troughs. The curvatures of the crests and troughs increase with an increase of the primary wave's steepness and the resonant wave's propagation distance. For primary waves with small steepness, the crest and trough lines will eventually split, as long as the propagation distance is sufficiently large. The crest line is split into a longer segment with a larger wave crest and a shorter one with a smaller wave crest.

Longest segment of balanced parentheses: an exercise in program inversion in a segment problem

Abstract Given a string of parentheses, the task is to find the longest consecutive segment that is balanced, in linear time. We find this problem interesting because it involves a combination of techniques: the usual approach for solving segment problems and a theorem for constructing the inverse of a function—through which we derive an instance of shift-reduce parsing.

The Prevalence and Predictive Factors of Painful Tonic Spasm in Patients with Neuromyelitis Optica Spectrum Disorder (NMOSD)

Background: Painful tonic spasm (PTS) is a complication frequently observed in patients with neuromyelitis optica spectrum disorder (NMOSD). Objective: To identify the prevalence of PTS, compare the factors associated with the occurrence of PTS, define the characteristics of PTS, and correlate the medication and prognostic factors with good recovery from PTS in patients with NMOSD. Materials and Methods: A retrospective study was performed in patients with definite NMOSD in the Prasat Neurological Institute between January 1, 2014 and December 31, 2018. The prevalence and characteristics of PTS were explored. The characteristics and factors associated with the occurrence of PTS were investigated. Moreover, the factors associated with PTS recovery and pain medications were further analyzed in the present study. Results: The prevalence of PTS in patients with NMOSD was 37.81%. The factors associated with the occurrence of PTS were the presence of acute myelitis (p=0.002, OR 39.00, 95% CI 3.89 to 391.23), and tobacco use (p=0.048, OR 13.38, 95% CI 1.02 to 175.52). In the subgroup analyses of the factors associated with PTS recovery, plasma exchange (p=0.007, OR 24.70, 95% CI 2.43 to 251.57), and Expanded Disability Status Scale range 1.0 to 4.5 (p=0.008, OR 6.92, 95% CI 1.67 to 28.65) were related to the recovery from PTS. While non-recovery was correlated with older age at last visit (p=0.013, OR 1.09, 95% CI 1.02 to 1.17) and longer segments of cord lesions (p=0.016, OR 1.21, 95% CI 1.04 to 1.42). Conclusion: The present study supports that PTS is one of the common complications in patients with NMOSD in Thailand. The presence of acute myelitis and the tobacco use are associated with the presence of PTS. Plasma exchange treatment in the acute phase of NMOSD may be associated with good recovery from PTS, and longer segments of spinal cord lesions is correlated with poor recovery outcomes. The control of these factors may prevent the occurrence of PTS or at least facilitate the recovery from PTS in these patients. Keywords: Neuromyelitis optica spectrum disorder (NMOSD), Painful tonic spasm (PTS)

A Two-State Model Describes the Temperature-Dependent Conformational Equilibrium in the Alanine-Rich Domains in Elastin.

Elastin is the insoluble elastomeric protein that provides extensibility and resilience to vertebrate tissues. Limited high-resolution structural data for elastin are notably complex. To access this information, this protein is considered in the simplified context of its two general domain types, that is, hydrophobic (HP) and crosslinking (CL). The question of elastin's structure-function has directed the focus of nearly all previous studies in the literature to the unique repeating sequences characteristic of this protein, found primarily in the HP domains. The CL domains were assumed to play a very limited role in biological elasticity due in part to the significant α-helical character that was (incorrectly) predicted for these regions. In this study, the conformational heterogeneity of alanines in native elastin's CL domains is examined in the context of helix-coil transition theory (HCTT) using solid-state nuclear magnetic resonance (SSNMR) spectroscopy in tandem with strategic isotopic labeling. Helix and coil populations are observed at all temperatures, but the former increases significantly at lower temperatures. Below the glass transition temperature (Tg), two major populations of alanines in the CL regions are resolved by two-dimensional SSNMR; one-dimensional methods are used for characterization in nativelike conditions. The spectra of 13CO-Ala in the CL regions are simulated using an HCTT-based statistical mechanical representation. Below Tg, longer segments with significant helical probabilities are consistent with the experimental data. At higher temperatures, the SSNMR lineshapes are best fit with a distribution of shorter (Ala)n segments, most in random coil. These results are used to refine a structure-function model for elastin in the context of HCTT, redirecting attention to the CL domains and their role in elasticity.

Intraluminal manipulator-assisted laparoscopic surgery for Hirschsprung disease

Abstract Background Total colonic aganglionosis (TCA) and long segment aganglionosis (LSA) are rare forms of Hirschsprung disease (HD). Laparoscopic resection of total or the longer segment of the colon has not been unestablished for these forms. Methods We retrospectively reviewed five infants with TCA or LSA who underwent the blunt tip dissector (BD) assisted laparoscopic Soave pull-through procedure, and patients’ characteristics and procedure outcomes were analyzed. Results One infant with TCA had Mowat–Wilson syndrome. Ileostomy for TCA and transanal catheter placement for LSA allowed full enteral feeding with good weight gain before operation. The BD manipulator used in the laparoscopic surgery was simple to use and powerful enough to create tension, expand operating space, and improve access to specific parts of the mesentery or pelvis. No intraoperative complications such as perforation or bleeding were noted; thus, the operation was completed uneventfully, without conversion to open surgery in all cases. All the patients had excellent cosmetic results, without late complications. Conclusions The BD manipulator is safe and effective to facilitate laparoscopy-assisted Soave pull-through procedure in children with TCA and LSA. This approach may be applied to the other pediatric diseases, which require resection of the longer segment of the colon.

Configuration of active site segments in lytic polysaccharide monooxygenases steers oxidative xyloglucan degradation

BackgroundLytic polysaccharide monooxygenases (LPMOs) are powerful enzymes that oxidatively cleave plant cell wall polysaccharides. LPMOs classified as fungal Auxiliary Activities family 9 (AA9) have been mainly studied for their activity towards cellulose; however, various members of this AA9 family have been also shown to oxidatively cleave hemicelluloses, in particularly xyloglucan (XG). So far, it has not been studied in detail how various AA9 LPMOs act in XG degradation, and in particular, how the mode-of-action relates to the structural configuration of these LPMOs.ResultsTwo Neurospora crassa (Nc) LPMOs were found to represent different mode-of-action towards XG. Interestingly, the configuration of active site segments of these LPMOs differed as well, with a shorter Segment 1 (−Seg1) and a longer Segment 2 (+Seg2) present in NcLPMO9C and the opposite for NcLPMO9M (+Seg1−Seg2). We confirmed that NcLPMO9C cleaved the non-reducing end of unbranched glucosyl residues within XG via the oxidation of the C4-carbon. In contrast, we found that the oxidative cleavage of the XG backbone by NcLPMO9M occurred next to both unbranched and substituted glucosyl residues. The latter are decorated with xylosyl, xylosyl–galactosyl and xylosyl–galactosyl–fucosyl units. The relationship between active site segments and the mode-of-action of these NcLPMOs was rationalized by a structure-based phylogenetic analysis of fungal AA9 LPMOs. LPMOs with a −Seg1+Seg2 configuration clustered together and appear to have a similar XG substitution-intolerant cleavage pattern. LPMOs with the +Seg1−Seg2 configuration also clustered together and are reported to display a XG substitution-tolerant cleavage pattern. A third cluster contained LPMOs with a −Seg1−Seg2 configuration and no oxidative XG activity.ConclusionsThe detailed characterization of XG degradation products released by LPMOs reveal a correlation between the configuration of active site segments and mode-of-action of LPMOs. In particular, oxidative XG-active LPMOs, which are tolerant and intolerant to XG substitutions are structurally and phylogenetically distinguished from XG-inactive LPMOs. This study contributes to a better understanding of the structure–function relationship of AA9 LPMOs.